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The map label for this gene is pfkA

Identifier: 15673315

GI number: 15673315

Start: 1371915

End: 1372937

Strand: Reverse

Name: pfkA

Synonym: L0002

Alternate gene names: 15673315

Gene position: 1372937-1371915 (Counterclockwise)

Preceding gene: 15673316

Following gene: 15673314

Centisome position: 58.04

GC content: 42.72

Gene sequence:

>1023_bases
ATGAAACGCATTGCAGTTTTGACTTCTGGTGGTGATGCCCCAGGAATGAATGCGGCTATTCGTGCAGTTGTTCGCAAAGC
AATTTCTGAAGGTATCGAAGTTTACGGTATCAATCACGGATATGCGGGCATGGTTGCGGGAGATATTTTCCCGCTTACGT
CAGCTTCAGTTGGTGATAAAATCGGTCGGGGTGGTACATTCTTGTATTCAGCACGCTACCCAGAATTTGCTCAAGTAGAA
GGACAACTTGCTGGGATTGAGCAACTTAAAAAATTCGGTATCGAAGGTGTCGTTGTAATCGGTGGTGATGGTTCTTATCA
TGGAGCTATGCGTCTTACAGAACATGGTTTCCCAGCTGTTGGACTTCCAGGAACAATCGATAACGATATCGTAGGAACTG
ATTTTACAATTGGATTTGATACAGCTGTTTCAACAGTTGTAGATGCCTTGGATAAAATCCGTGATACTTCATCATCACAT
AACCGTACTTTCGTTGTAGAAGTAATGGGACGTAATGCTGGAGATATCGCTTTGAATGCTGGTATCGCTGCTGGTGCAGA
TGATATTTGTATTCCAGAAAAAGAATTTAAGTTTGAAAACGTAGTTAACAACATTAACAAAGGCTACGAAAAAGGTAAAA
ATCACCACATCATCGTTCTTGCTGAAGGTGTAATGACTGGTGAAGAATTTGCTACAAAACTTAAAGAAGCTGGTTATAAA
GGAGACCTTCGCGTTTCTGTCCTTGGACACATCCAACGTGGTGGTTCACCAACAGCTCGTGACCGTGTTCTTGCTTCACG
TATGGGTGCTCGTGCCGTTGAATTGCTTCGTGATGGAATCGGTGGCGTAGCCGTTGGTATCCGTAATGAAGAACTTGTAG
AAAGTCCAATTCTCGGAACAGCTGAAGAAGGAGCACTCTTTAGTTTGACTACTGAAGGCGGAATCAAAGTAAATAACCCG
CATAAAGCTGGTCTTGAACTTTACCGTCTTAACTCAGCACTCAACAATCTTAACCTTAACTAA

Upstream 100 bases:

>100_bases
TACGTGATATAATGAACTAGACAACAAATGAACATGGTAAAAATATTAATAACGTTTACGTATTTTTGCTATGTATCTCA
ATTTCGGAGGACAGATTTAA

Downstream 100 bases:

>100_bases
GTTACTGCAAATCTGTTTCTAGTTAAGTGTTAAACGCATAATTAGGGCAGAGATATATAATTAATCATTATAGGAGAAAA
ACACAAAATGAATAAACGTG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGIEVYGINHGYAGMVAGDIFPLTSASVGDKIGRGGTFLYSARYPEFAQVE
GQLAGIEQLKKFGIEGVVVIGGDGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDFTIGFDTAVSTVVDALDKIRDTSSSH
NRTFVVEVMGRNAGDIALNAGIAAGADDICIPEKEFKFENVVNNINKGYEKGKNHHIIVLAEGVMTGEEFATKLKEAGYK
GDLRVSVLGHIQRGGSPTARDRVLASRMGARAVELLRDGIGGVAVGIRNEELVESPILGTAEEGALFSLTTEGGIKVNNP
HKAGLELYRLNSALNNLNLN

Sequences:

>Translated_340_residues
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGIEVYGINHGYAGMVAGDIFPLTSASVGDKIGRGGTFLYSARYPEFAQVE
GQLAGIEQLKKFGIEGVVVIGGDGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDFTIGFDTAVSTVVDALDKIRDTSSSH
NRTFVVEVMGRNAGDIALNAGIAAGADDICIPEKEFKFENVVNNINKGYEKGKNHHIIVLAEGVMTGEEFATKLKEAGYK
GDLRVSVLGHIQRGGSPTARDRVLASRMGARAVELLRDGIGGVAVGIRNEELVESPILGTAEEGALFSLTTEGGIKVNNP
HKAGLELYRLNSALNNLNLN
>Mature_340_residues
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGIEVYGINHGYAGMVAGDIFPLTSASVGDKIGRGGTFLYSARYPEFAQVE
GQLAGIEQLKKFGIEGVVVIGGDGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDFTIGFDTAVSTVVDALDKIRDTSSSH
NRTFVVEVMGRNAGDIALNAGIAAGADDICIPEKEFKFENVVNNINKGYEKGKNHHIIVLAEGVMTGEEFATKLKEAGYK
GDLRVSVLGHIQRGGSPTARDRVLASRMGARAVELLRDGIGGVAVGIRNEELVESPILGTAEEGALFSLTTEGGIKVNNP
HKAGLELYRLNSALNNLNLN

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI266453768, Length=341, Percent_Identity=39.0029325513196, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI266453748, Length=341, Percent_Identity=39.0029325513196, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI4505749, Length=341, Percent_Identity=39.0029325513196, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI266453619, Length=341, Percent_Identity=39.0029325513196, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI11321601, Length=308, Percent_Identity=42.5324675324675, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI48762920, Length=312, Percent_Identity=41.025641025641, Blast_Score=191, Evalue=6e-49,
Organism=Escherichia coli, GI1790350, Length=303, Percent_Identity=58.4158415841584, Blast_Score=331, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI25147584, Length=343, Percent_Identity=38.1924198250729, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=35.7142857142857, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI72003601, Length=288, Percent_Identity=31.9444444444444, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321679, Length=337, Percent_Identity=37.3887240356083, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6323861, Length=340, Percent_Identity=35.5882352941176, Blast_Score=180, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24652337, Length=339, Percent_Identity=40.4129793510324, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17647809, Length=339, Percent_Identity=40.4129793510324, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI28573326, Length=339, Percent_Identity=39.8230088495575, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_LACLA (Q07636)

Other databases:

- EMBL:   L07920
- EMBL:   AE005176
- PIR:   A40620
- RefSeq:   NP_267489.1
- ProteinModelPortal:   Q07636
- SMR:   Q07636
- GeneID:   1114983
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0002
- NMPDR:   fig|272623.1.peg.1368
- HOGENOM:   HBG405044
- OMA:   QKENINA
- ProtClustDB:   PRK03202
- BioCyc:   LLAC272623:L0002-MONOMER
- BioCyc:   MetaCyc:MONOMER-13044
- BRENDA:   2.7.1.11
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 35806; Mature: 35806

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 127-127 BINDING 162-162 BINDING 244-244 BINDING 250-250 BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGIEVYGINHGYAGMVAGDIFPLTSASVGDK
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCEECCCCCHHCCC
IGRGGTFLYSARYPEFAQVEGQLAGIEQLKKFGIEGVVVIGGDGSYHGAMRLTEHGFPAV
CCCCCEEEEECCCCCHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GLPGTIDNDIVGTDFTIGFDTAVSTVVDALDKIRDTSSSHNRTFVVEVMGRNAGDIALNA
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEC
GIAAGADDICIPEKEFKFENVVNNINKGYEKGKNHHIIVLAEGVMTGEEFATKLKEAGYK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
GDLRVSVLGHIQRGGSPTARDRVLASRMGARAVELLRDGIGGVAVGIRNEELVESPILGT
CCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCC
AEEGALFSLTTEGGIKVNNPHKAGLELYRLNSALNNLNLN
CCCCCEEEEECCCCEEECCCCHHCCEEEEHHHHHHCCCCC
>Mature Secondary Structure
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGIEVYGINHGYAGMVAGDIFPLTSASVGDK
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCEECCCCCHHCCC
IGRGGTFLYSARYPEFAQVEGQLAGIEQLKKFGIEGVVVIGGDGSYHGAMRLTEHGFPAV
CCCCCEEEEECCCCCHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GLPGTIDNDIVGTDFTIGFDTAVSTVVDALDKIRDTSSSHNRTFVVEVMGRNAGDIALNA
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEC
GIAAGADDICIPEKEFKFENVVNNINKGYEKGKNHHIIVLAEGVMTGEEFATKLKEAGYK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
GDLRVSVLGHIQRGGSPTARDRVLASRMGARAVELLRDGIGGVAVGIRNEELVESPILGT
CCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCC
AEEGALFSLTTEGGIKVNNPHKAGLELYRLNSALNNLNLN
CCCCCEEEEECCCCEEECCCCHHCCEEEEHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8478320; 11337471