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The map label for this gene is gadC

Identifier: 15673273

GI number: 15673273

Start: 1325256

End: 1326767

Strand: Reverse

Name: gadC

Synonym: L125001

Alternate gene names: 15673273

Gene position: 1326767-1325256 (Counterclockwise)

Preceding gene: 15673274

Following gene: 15673272

Centisome position: 56.09

GC content: 34.72

Gene sequence:

>1512_bases
ATGAATCAAAAAAAAATATCATTATTCGGTTTTTTTGCATTAACCGCTTCAATGGTTTTGACTGTCTATGAGTATCCAAC
TTTTGCCACGTCAAAATTACATTTGGTGTTCTTTTTACTTCTCGGAGGACTACTCTGGTTTTTGCCCGTAGCGCTCTGTG
CAGCAGAAATGGCGACGGTTGAAGGTTGGAAAAATGGTGGAATTTTTAGTTGGGTGAGCCAAACTTTAGGGGAGCGCTTT
GGTTTTGCAGCCATATTTTTTCAGTGGTTTCAAATTACAGTTGGCTTTGTCACTATGATCTATTTCATTTTAGGGGCCCT
CTCTTATGTGTTAAATTTTCAGGCACTTAATACAGATCCATTGATTAAATTTATTGGTTTACTAATCATTTTTTGGGGAT
TGACATTTTCTCAGTTAGGTGGGACACAAAGAACTGCTAAATTGGTAAAAGCTGGTTTTGTAGTTGGGATAGTGATTCCA
TCGATTATCTTGTTTGGATTAGCTGCGGCATATTTTATCGGAGGTAATCCTATAGAGATACCAATTAATAAGCATGCTTT
TGTACCAGATTTTTCACAAGTATCAACTTTAGTAGTTTTTGTTTCTTTTATTCTTGCTTATATGGGGGTAGAAGCTTCAG
CTTCGCACATTAATGAACTTGAAAATCCAAAGAGAAATTATCCCTTAGCAATGATTTTATTAGTAATATTAGCTATTTCT
TTAGATGCCATAGGTGGATTTTCTGTAGCAGCGGTTATTCCTCAAAAAGACTTATCATTAAGTGCAGGGGTAATTCAAAC
TTTTCAAACGTTAATCTTACATTTTAATCATCATTTGGGATGGTTAGTTAAAGTAATTGCACTAATGATTGCCTTTGGGG
TTATGGGAGAAGTGAGTTCATGGGTTGTTGGTCCTTCTAGAGGGATGTTTGCAGCAGCACAAAGAGGCTTATTACCAAAA
TTTTTACGTAAAACAAATACGCATGAAGTCCCTGTTCCTTTAGTTATGATTCAAGGAATCATTGTTACACTTTGGGGCGC
TGTATTAACTTTTGGAGGAGGAGGAAATAATTTATCTTTCTTAGTTGCCATTTCACTGACAGTAGTGATTTATTTGGTAG
GTTATCTCTTATTCTTTATTGGTTACTTTGTTTTAATCTATAAAAAACAAAATTTAAAGCGCACTTATAATGTTCCAGGT
AAAAGAGTTGGAAAAACAATCATTGCGGGAATTGGATTTTTATTATCAATTTTTGCTCTATTTATTTCCTTTGTTCCTCC
AGCATCAATTGCGAAAAATGAGACTCATACTTACCAAATGATACTTCTTATAAGTTTTGTTGTGACTGCTATCTTGCCAT
TTATTGTTTATGAATTGCATGATAAAAGGGGACATGATACTATTGAAGAACCAAGGCACTTTAAAGCAAGAGATGTGAAC
CCCGCGATTTATCCAGCAGCTCGTGGTGAGCATCATATTATTAAAAAAGAAGAACATATCTTAAAACATTAA

Upstream 100 bases:

>100_bases
TTTTTTTATTCTGGAGCATATACTAGAAAATGAAGATGAGTTGTATGAGGCTATATAACATGTCTTTTAAAAAATTCAAT
CATAAATATAAGGAGGTATG

Downstream 100 bases:

>100_bases
AAAATTGGAGGATGTACCTATGTTATACGGAAAAGAAAATCGCGATGAAGCAGAGTTCTTGGAACCAATTTTTGGTTCAG
AAAGTGAACAAGTGGATTTG

Product: glutamate-gamma-aminobutyrate

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MNQKKISLFGFFALTASMVLTVYEYPTFATSKLHLVFFLLLGGLLWFLPVALCAAEMATVEGWKNGGIFSWVSQTLGERF
GFAAIFFQWFQITVGFVTMIYFILGALSYVLNFQALNTDPLIKFIGLLIIFWGLTFSQLGGTQRTAKLVKAGFVVGIVIP
SIILFGLAAAYFIGGNPIEIPINKHAFVPDFSQVSTLVVFVSFILAYMGVEASASHINELENPKRNYPLAMILLVILAIS
LDAIGGFSVAAVIPQKDLSLSAGVIQTFQTLILHFNHHLGWLVKVIALMIAFGVMGEVSSWVVGPSRGMFAAAQRGLLPK
FLRKTNTHEVPVPLVMIQGIIVTLWGAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFIGYFVLIYKKQNLKRTYNVPG
KRVGKTIIAGIGFLLSIFALFISFVPPASIAKNETHTYQMILLISFVVTAILPFIVYELHDKRGHDTIEEPRHFKARDVN
PAIYPAARGEHHIIKKEEHILKH

Sequences:

>Translated_503_residues
MNQKKISLFGFFALTASMVLTVYEYPTFATSKLHLVFFLLLGGLLWFLPVALCAAEMATVEGWKNGGIFSWVSQTLGERF
GFAAIFFQWFQITVGFVTMIYFILGALSYVLNFQALNTDPLIKFIGLLIIFWGLTFSQLGGTQRTAKLVKAGFVVGIVIP
SIILFGLAAAYFIGGNPIEIPINKHAFVPDFSQVSTLVVFVSFILAYMGVEASASHINELENPKRNYPLAMILLVILAIS
LDAIGGFSVAAVIPQKDLSLSAGVIQTFQTLILHFNHHLGWLVKVIALMIAFGVMGEVSSWVVGPSRGMFAAAQRGLLPK
FLRKTNTHEVPVPLVMIQGIIVTLWGAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFIGYFVLIYKKQNLKRTYNVPG
KRVGKTIIAGIGFLLSIFALFISFVPPASIAKNETHTYQMILLISFVVTAILPFIVYELHDKRGHDTIEEPRHFKARDVN
PAIYPAARGEHHIIKKEEHILKH
>Mature_503_residues
MNQKKISLFGFFALTASMVLTVYEYPTFATSKLHLVFFLLLGGLLWFLPVALCAAEMATVEGWKNGGIFSWVSQTLGERF
GFAAIFFQWFQITVGFVTMIYFILGALSYVLNFQALNTDPLIKFIGLLIIFWGLTFSQLGGTQRTAKLVKAGFVVGIVIP
SIILFGLAAAYFIGGNPIEIPINKHAFVPDFSQVSTLVVFVSFILAYMGVEASASHINELENPKRNYPLAMILLVILAIS
LDAIGGFSVAAVIPQKDLSLSAGVIQTFQTLILHFNHHLGWLVKVIALMIAFGVMGEVSSWVVGPSRGMFAAAQRGLLPK
FLRKTNTHEVPVPLVMIQGIIVTLWGAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFIGYFVLIYKKQNLKRTYNVPG
KRVGKTIIAGIGFLLSIFALFISFVPPASIAKNETHTYQMILLISFVVTAILPFIVYELHDKRGHDTIEEPRHFKARDVN
PAIYPAARGEHHIIKKEEHILKH

Specific function: Involved in glutamate-dependent acid resistance. May be involved in antiport of glutamate and glutamate-gamma- aminobutyrate (GABA)

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Glutamate:GABA antiporter (GGA) (TC 2.A.3.7) family

Homologues:

Organism=Escherichia coli, GI1787768, Length=502, Percent_Identity=52.191235059761, Blast_Score=530, Evalue=1e-151,
Organism=Escherichia coli, GI48994921, Length=427, Percent_Identity=20.6088992974239, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI87082380, Length=482, Percent_Identity=21.9917012448133, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI2367353, Length=280, Percent_Identity=23.2142857142857, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GADC_LACLA (Q9CG19)

Other databases:

- EMBL:   AE005176
- EMBL:   AB010789
- PIR:   C86786
- RefSeq:   NP_267447.1
- GeneID:   1114940
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L125001
- NMPDR:   fig|272623.1.peg.1326
- HOGENOM:   HBG514881
- OMA:   MIAGRCK
- ProtClustDB:   CLSK876865
- BioCyc:   LLAC272623:L125001-MONOMER
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004759
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060
- TIGRFAMs:   TIGR00910

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 55566; Mature: 55566

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe958368)-; HASH(0xe9e0104)-; HASH(0xe24d414)-; HASH(0xde78da4)-; HASH(0xe874468)-; HASH(0xe4efed4)-; HASH(0xe665108)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCHHHHHCH
>Mature Secondary Structure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CCCCCCCCCCCCCCCCHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11337471; 10411264