Click here to switch to the map view.

The map label for this gene is holB

Identifier: 15672381

GI number: 15672381

Start: 404636

End: 405496

Strand: Direct

Name: holB

Synonym: L0280

Alternate gene names: 15672381

Gene position: 404636-405496 (Clockwise)

Preceding gene: 15672380

Following gene: 15672382

Centisome position: 17.11

GC content: 32.75

Gene sequence:

>861_bases
GTGGAAATAAAAGATATTCAGCCTCAACTTTATAAGCAGTTCTCAACGATTTTGCAACATGGAAAATTAAGTCATGCTTA
TTTATTTTCAGGGGGATTTGGTTCTTTTGAATTAGCAATCTGGCTGAGCCAAGCCTTATTTTGTGAGAATCCTGAAAATG
CTTTGCCTTGTGGTCATTGTCGTTCCTGTCGTCTTGTTGCCCAACAAGAATTTACCGACTTACATATCGTTGAACCTGAT
GGACAAACGATTAAAACAGCACAAATTCGAGAACTGACACAAGTTTTCAATGAATCTGGTTATGAGGGAAATCAACAAGT
GCTTTTAATTAAGGAAGCTGAAAAAATGCATCCTAATGCAGCAAATGCTTTATTGAAATCAATTGAAGAACCAGAAACAG
AAATTGTTGTTTTTTTACTGACAGACAATGAAAATATGATTTTACAAACAATAAAATCACGGACACAAATTATTAATTTT
CCAAAAAATGTTCAATACTTACAAGATTTTTTGGAAAAGAATGGTATTTTAAAAACACAAGCAGAACTTCTTGCGGAAAT
CTGTAATTCGACTGACAAAGCATTAGAATTAGCTCAGCAGAGCTGGTTTAACGAAGGGCTTCAAAGACTTCAACAATTTG
TAAAACTTCTAAAAACATCTGCTGACGAGGCGTTCTTGTATTTGAAAGAATTAGTCGAAATTTTTGATGACAAAGAAAAA
CAAAATCAAGCGTTTGAACTTTTATTACAACTTTTTAATCAAGAAAGAATGAGTCAGGAACTTTTAAAAACATTTCAGGC
AATAAAAATGTGGAAAAGCAATGTCCGATTTGAGTCAAGTTTAACTTTTCTTGTTTTATGA

Upstream 100 bases:

>100_bases
GCTAAAAAATACTTACATTTTTCAAAAAATGAGGATGTTAAATAGTTTTTTTAATTTTGTTATTTAGACTAGATTTGGTA
AATATTTAGAAAGGTTTCTT

Downstream 100 bases:

>100_bases
AACTTGTCAAACTTATCCTTTGATTGAAAAATCTTATATCAGGAGAATATTATGATTTATGAAATAAAATTTGCACATGG
CGAATCAAATGTATTTGCAA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MEIKDIQPQLYKQFSTILQHGKLSHAYLFSGGFGSFELAIWLSQALFCENPENALPCGHCRSCRLVAQQEFTDLHIVEPD
GQTIKTAQIRELTQVFNESGYEGNQQVLLIKEAEKMHPNAANALLKSIEEPETEIVVFLLTDNENMILQTIKSRTQIINF
PKNVQYLQDFLEKNGILKTQAELLAEICNSTDKALELAQQSWFNEGLQRLQQFVKLLKTSADEAFLYLKELVEIFDDKEK
QNQAFELLLQLFNQERMSQELLKTFQAIKMWKSNVRFESSLTFLVL

Sequences:

>Translated_286_residues
MEIKDIQPQLYKQFSTILQHGKLSHAYLFSGGFGSFELAIWLSQALFCENPENALPCGHCRSCRLVAQQEFTDLHIVEPD
GQTIKTAQIRELTQVFNESGYEGNQQVLLIKEAEKMHPNAANALLKSIEEPETEIVVFLLTDNENMILQTIKSRTQIINF
PKNVQYLQDFLEKNGILKTQAELLAEICNSTDKALELAQQSWFNEGLQRLQQFVKLLKTSADEAFLYLKELVEIFDDKEK
QNQAFELLLQLFNQERMSQELLKTFQAIKMWKSNVRFESSLTFLVL
>Mature_286_residues
MEIKDIQPQLYKQFSTILQHGKLSHAYLFSGGFGSFELAIWLSQALFCENPENALPCGHCRSCRLVAQQEFTDLHIVEPD
GQTIKTAQIRELTQVFNESGYEGNQQVLLIKEAEKMHPNAANALLKSIEEPETEIVVFLLTDNENMILQTIKSRTQIINF
PKNVQYLQDFLEKNGILKTQAELLAEICNSTDKALELAQQSWFNEGLQRLQQFVKLLKTSADEAFLYLKELVEIFDDKEK
QNQAFELLLQLFNQERMSQELLKTFQAIKMWKSNVRFESSLTFLVL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=136, Percent_Identity=36.0294117647059, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787341, Length=174, Percent_Identity=32.183908045977, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 33002; Mature: 33002

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKDIQPQLYKQFSTILQHGKLSHAYLFSGGFGSFELAIWLSQALFCENPENALPCGHC
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
RSCRLVAQQEFTDLHIVEPDGQTIKTAQIRELTQVFNESGYEGNQQVLLIKEAEKMHPNA
CHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHCCCH
ANALLKSIEEPETEIVVFLLTDNENMILQTIKSRTQIINFPKNVQYLQDFLEKNGILKTQ
HHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHH
AELLAEICNSTDKALELAQQSWFNEGLQRLQQFVKLLKTSADEAFLYLKELVEIFDDKEK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHH
QNQAFELLLQLFNQERMSQELLKTFQAIKMWKSNVRFESSLTFLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEEEEC
>Mature Secondary Structure
MEIKDIQPQLYKQFSTILQHGKLSHAYLFSGGFGSFELAIWLSQALFCENPENALPCGHC
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
RSCRLVAQQEFTDLHIVEPDGQTIKTAQIRELTQVFNESGYEGNQQVLLIKEAEKMHPNA
CHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHCCCH
ANALLKSIEEPETEIVVFLLTDNENMILQTIKSRTQIINFPKNVQYLQDFLEKNGILKTQ
HHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHH
AELLAEICNSTDKALELAQQSWFNEGLQRLQQFVKLLKTSADEAFLYLKELVEIFDDKEK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHH
QNQAFELLLQLFNQERMSQELLKTFQAIKMWKSNVRFESSLTFLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]