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The map label for this gene is hemB

Identifier: 15668824

GI number: 15668824

Start: 572446

End: 573453

Strand: Direct

Name: hemB

Synonym: MJ0643

Alternate gene names: 15668824

Gene position: 572446-573453 (Clockwise)

Preceding gene: 15668823

Following gene: 15668825

Centisome position: 34.38

GC content: 36.31

Gene sequence:

>1008_bases
TTGTTTAAAATAAATTTGGGTGATTTTATGCTGATAAGGCCAAGAAGATTAAGAAAAAACCAAAAAATTAGAGATTTAGT
TAGAGAAACTATATTAACAAAAAATGACTTAATTATGCCAATTTTTGTAGATGAAAATTTAAAAGGGAATGAGAAGAAGG
AGATTAGCTCAATGCCTAATCAGTATAGGTTTAGTGTGGAAGGGGCTATAGAAGAAGCAAAAGAAATAGCTGATTTAGGC
ATTCCAGCTGTAATATTATTTGGTATTCCAAAGCATAAGGATGAGATAGCAAGCTCTGCCTACGATAAAAATGGAGTTGT
TCAAAGAACTATAAGGGGAATTAAAGAAGAGTTAGGGGATGAGCTTTTAGTTATTGCCGATTGTTGTTTATGCGAATACA
CAAGCCATGGACACTGTGGAATAGTTAAAGATGGAAAGATTTTGAACGATGCCACACTCCCAATATTGGCAAAGATAGCT
TTATCCTATGCAGATGCTGGTGTTGATATTGTCGCTCCTTCAGACATGATGGATGGAAGAGTTAGGGCTATAAGGGAGAT
TTTAGAAGAAAATGGATATGATGATGTTGCTATAATGAGTTACTCAGCTAAATATGCCTCATCATTTTACGGCCCGTTTA
GAGAAGCGGCTGAAAGTGCCCCTAAATTTGGAGATAGGAAGAGTTATCAGATGGACATAGGAAACGCAAGAGAGGCTTTA
AAAGAAATTGCATTGGATATAGAGGAGGGTGCTGATTTAATTTTGGTTAAGCCAGCTTTGCCTTATTTGGATATAATAAG
GATGGCTAAGGACAGGTTTGATGTGCCTATTGGTGGATACTGCGTTAGCGGAGAGTATGCAATGGTTGAAGCAGCAGCAA
GAAATGGATGGTTAGATAGAGAAAAAGTAATTTATGAAATACTGTTAAGTATAAAAAGGGCTGGAGCTGATTTTATTATA
ACATATTGGGCTAAGGAGGTTGCTGAAATAGGACTTTCACAGGAATAA

Upstream 100 bases:

>100_bases
GAAACACTAAAGTGCTCTGAAGTTATGGAGGAATATGTGAAAATTATCCACCCAAAAATTTATCAAAATGGAGATAATGA
TTTAAAATACTTTATAAAAG

Downstream 100 bases:

>100_bases
AATTTATAAAGGAAATTGATGCCTTTAGGCATCTAAGTACCTTATTAAAACCTACAAACTGTGAAAGTCCTATTAAAAAT
TATTGTAGGGAGGGCTATGA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MFKINLGDFMLIRPRRLRKNQKIRDLVRETILTKNDLIMPIFVDENLKGNEKKEISSMPNQYRFSVEGAIEEAKEIADLG
IPAVILFGIPKHKDEIASSAYDKNGVVQRTIRGIKEELGDELLVIADCCLCEYTSHGHCGIVKDGKILNDATLPILAKIA
LSYADAGVDIVAPSDMMDGRVRAIREILEENGYDDVAIMSYSAKYASSFYGPFREAAESAPKFGDRKSYQMDIGNAREAL
KEIALDIEEGADLILVKPALPYLDIIRMAKDRFDVPIGGYCVSGEYAMVEAAARNGWLDREKVIYEILLSIKRAGADFII
TYWAKEVAEIGLSQE

Sequences:

>Translated_335_residues
MFKINLGDFMLIRPRRLRKNQKIRDLVRETILTKNDLIMPIFVDENLKGNEKKEISSMPNQYRFSVEGAIEEAKEIADLG
IPAVILFGIPKHKDEIASSAYDKNGVVQRTIRGIKEELGDELLVIADCCLCEYTSHGHCGIVKDGKILNDATLPILAKIA
LSYADAGVDIVAPSDMMDGRVRAIREILEENGYDDVAIMSYSAKYASSFYGPFREAAESAPKFGDRKSYQMDIGNAREAL
KEIALDIEEGADLILVKPALPYLDIIRMAKDRFDVPIGGYCVSGEYAMVEAAARNGWLDREKVIYEILLSIKRAGADFII
TYWAKEVAEIGLSQE
>Mature_335_residues
MFKINLGDFMLIRPRRLRKNQKIRDLVRETILTKNDLIMPIFVDENLKGNEKKEISSMPNQYRFSVEGAIEEAKEIADLG
IPAVILFGIPKHKDEIASSAYDKNGVVQRTIRGIKEELGDELLVIADCCLCEYTSHGHCGIVKDGKILNDATLPILAKIA
LSYADAGVDIVAPSDMMDGRVRAIREILEENGYDDVAIMSYSAKYASSFYGPFREAAESAPKFGDRKSYQMDIGNAREAL
KEIALDIEEGADLILVKPALPYLDIIRMAKDRFDVPIGGYCVSGEYAMVEAAARNGWLDREKVIYEILLSIKRAGADFII
TYWAKEVAEIGLSQE

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family

Homologues:

Organism=Homo sapiens, GI189083849, Length=328, Percent_Identity=40.8536585365854, Blast_Score=248, Evalue=8e-66,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=51.25, Blast_Score=307, Evalue=7e-85,
Organism=Saccharomyces cerevisiae, GI6321398, Length=310, Percent_Identity=44.5161290322581, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI21358291, Length=302, Percent_Identity=41.7218543046358, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM2_METJA (Q60178)

Other databases:

- EMBL:   L77117
- PIR:   C64380
- RefSeq:   NP_247627.1
- ProteinModelPortal:   Q60178
- SMR:   Q60178
- GeneID:   1451509
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0643
- NMPDR:   fig|243232.1.peg.664
- TIGR:   MJ0643
- HOGENOM:   HBG285270
- OMA:   FTSHGHD
- ProtClustDB:   PRK09283
- BioCyc:   MJAN243232:MJ_0643-MONOMER
- BRENDA:   4.2.1.24
- InterPro:   IPR001731
- InterPro:   IPR013785
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11458
- PIRSF:   PIRSF001415
- PRINTS:   PR00144

Pfam domain/function: PF00490 ALAD

EC number: =4.2.1.24

Molecular weight: Translated: 37397; Mature: 37397

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: ACT_SITE 204-204 ACT_SITE 257-257 BINDING 214-214 BINDING 226-226 BINDING 283-283 BINDING 322-322

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKINLGDFMLIRPRRLRKNQKIRDLVRETILTKNDLIMPIFVDENLKGNEKKEISSMPN
CEEECCCCEEEECCHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCC
QYRFSVEGAIEEAKEIADLGIPAVILFGIPKHKDEIASSAYDKNGVVQRTIRGIKEELGD
CEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCC
ELLVIADCCLCEYTSHGHCGIVKDGKILNDATLPILAKIALSYADAGVDIVAPSDMMDGR
CEEEEHHHHHHHCCCCCCEEEEECCCEECCCHHHHHHHHHHHHHCCCEEEECCCHHHCHH
VRAIREILEENGYDDVAIMSYSAKYASSFYGPFREAAESAPKFGDRKSYQMDIGNAREAL
HHHHHHHHHHCCCCCEEEEEECHHHHHHHCCHHHHHHHHCCCCCCCCCEEECCCHHHHHH
KEIALDIEEGADLILVKPALPYLDIIRMAKDRFDVPIGGYCVSGEYAMVEAAARNGWLDR
HHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEECCCHHEEHHHHHCCCCCH
EKVIYEILLSIKRAGADFIITYWAKEVAEIGLSQE
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFKINLGDFMLIRPRRLRKNQKIRDLVRETILTKNDLIMPIFVDENLKGNEKKEISSMPN
CEEECCCCEEEECCHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCC
QYRFSVEGAIEEAKEIADLGIPAVILFGIPKHKDEIASSAYDKNGVVQRTIRGIKEELGD
CEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCC
ELLVIADCCLCEYTSHGHCGIVKDGKILNDATLPILAKIALSYADAGVDIVAPSDMMDGR
CEEEEHHHHHHHCCCCCCEEEEECCCEECCCHHHHHHHHHHHHHCCCEEEECCCHHHCHH
VRAIREILEENGYDDVAIMSYSAKYASSFYGPFREAAESAPKFGDRKSYQMDIGNAREAL
HHHHHHHHHHCCCCCEEEEEECHHHHHHHCCHHHHHHHHCCCCCCCCCEEECCCHHHHHH
KEIALDIEEGADLILVKPALPYLDIIRMAKDRFDVPIGGYCVSGEYAMVEAAARNGWLDR
HHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEECCCHHEEHHHHHCCCCCH
EKVIYEILLSIKRAGADFIITYWAKEVAEIGLSQE
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087