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The map label for this gene is pilU [H]

Identifier: 15607093

GI number: 15607093

Start: 1516861

End: 1517973

Strand: Direct

Name: pilU [H]

Synonym: aq_2151

Alternate gene names: 15607093

Gene position: 1516861-1517973 (Clockwise)

Preceding gene: 15607092

Following gene: 15607094

Centisome position: 97.78

GC content: 41.51

Gene sequence:

>1113_bases
TTGCTGGATCAAATATTGAGGAAAGCACTGGAGAAGAAGGCAAGTGATATACATCTAAAAGTTGGATCTAAACCTGTAAT
CAGAACACCTGATGGGCTAGACACTCTTGAAGAGTTCCCCCCTATTGACATTGATCTTTTTGAGGTATTTGTTAAAGAAA
TACTTGAACATCATCCTAAAAAGAAAAAAGAACTTGAAGAACAAGGGCAGGTTGACCTCTCCTACGCTATCCCTAAGGTT
TCCCGCTTCAGGGTAAACCTTTACAAACAAAGAGGAACCTACGCTATGGCCCTCAGGGTCATACCCTTCGACATTCCCGA
ATTCAAAAAATTAAACTTACCGCCGGTAATGCTCCAAACTGCTCTCAAACATTCAGCAGGAATGATACTCGTTACCGGAC
CCACGGGTTCAGGTAAGTCTACAACCATAGCTTCGATAATAAACGAAATAAACAAACGCTTGAGTAGAGTGATAATTACA
ATTGAAGATCCAATAGAGTACCTCTTTAAAGATATACAGTCTTTTATAGCACAAAGAGAAGTAGGGTGGGATACTGAAAG
TTTCTTCCTGGGTTTAAGGGCTGCTCTCAGAGAGGACCCGGACATAATATTTGTCGGAGAAATAAGAGACACGGAGACGG
CCAAAACTGCACTCCACGCTGCAGAAACAGGACACTTAGTATTCTCTACACTGCACACACTCGACGCAGTTGAAACTATA
AACAGGTTCATAGGTATGTTTCCATTGGAGCACAGAGATCAAATAAGACAGATGCTCGCCTCCACGCTTATAGCCGTGTA
CTCCCAGAGGTTAGTGTCCAAAAAAAATGGTGGAGGTAAAGTTCCTGTAGTAGAAATAATGATAATGACACAAACTATAA
GGGAAGCCATACTCGAAAACAGACCACAGGACATCCCTGAACTCATAGAAAAGGGAAGATTAGTGTACGGTTCTCAAACC
TTTGACCAGCATCTCGAAGAACTATACAGAAAAGGCTTAATAGATCTAGAAACCGCACTCCTTTACGCAAGAAGACCTGC
AGATCTCGAGCTAAGGCTGAAAGGTATATCCACCGATGAAGATATTATAGAAGGTGATAATTACTTAACATGA

Upstream 100 bases:

>100_bases
AAGAGACTTGGTCAAGGCAAGGAGCAAGTGAAAAAGTACCTTCAAGAACACCCCGAATTAATTGATGAAATAGATCAAAA
AGTAAGGGAGGCTGCAGGAC

Downstream 100 bases:

>100_bases
AGATAGCCATAGACGGACCTTCAGCGAGCGGAAAAAGTACCGTTGCCAAAATAATCTCACAAAAATTAAACATTCCTTAC
TTAGAAACTGGTTTAGTATA

Product: twitching mobility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MLDQILRKALEKKASDIHLKVGSKPVIRTPDGLDTLEEFPPIDIDLFEVFVKEILEHHPKKKKELEEQGQVDLSYAIPKV
SRFRVNLYKQRGTYAMALRVIPFDIPEFKKLNLPPVMLQTALKHSAGMILVTGPTGSGKSTTIASIINEINKRLSRVIIT
IEDPIEYLFKDIQSFIAQREVGWDTESFFLGLRAALREDPDIIFVGEIRDTETAKTALHAAETGHLVFSTLHTLDAVETI
NRFIGMFPLEHRDQIRQMLASTLIAVYSQRLVSKKNGGGKVPVVEIMIMTQTIREAILENRPQDIPELIEKGRLVYGSQT
FDQHLEELYRKGLIDLETALLYARRPADLELRLKGISTDEDIIEGDNYLT

Sequences:

>Translated_370_residues
MLDQILRKALEKKASDIHLKVGSKPVIRTPDGLDTLEEFPPIDIDLFEVFVKEILEHHPKKKKELEEQGQVDLSYAIPKV
SRFRVNLYKQRGTYAMALRVIPFDIPEFKKLNLPPVMLQTALKHSAGMILVTGPTGSGKSTTIASIINEINKRLSRVIIT
IEDPIEYLFKDIQSFIAQREVGWDTESFFLGLRAALREDPDIIFVGEIRDTETAKTALHAAETGHLVFSTLHTLDAVETI
NRFIGMFPLEHRDQIRQMLASTLIAVYSQRLVSKKNGGGKVPVVEIMIMTQTIREAILENRPQDIPELIEKGRLVYGSQT
FDQHLEELYRKGLIDLETALLYARRPADLELRLKGISTDEDIIEGDNYLT
>Mature_370_residues
MLDQILRKALEKKASDIHLKVGSKPVIRTPDGLDTLEEFPPIDIDLFEVFVKEILEHHPKKKKELEEQGQVDLSYAIPKV
SRFRVNLYKQRGTYAMALRVIPFDIPEFKKLNLPPVMLQTALKHSAGMILVTGPTGSGKSTTIASIINEINKRLSRVIIT
IEDPIEYLFKDIQSFIAQREVGWDTESFFLGLRAALREDPDIIFVGEIRDTETAKTALHAAETGHLVFSTLHTLDAVETI
NRFIGMFPLEHRDQIRQMLASTLIAVYSQRLVSKKNGGGKVPVVEIMIMTQTIREAILENRPQDIPELIEKGRLVYGSQT
FDQHLEELYRKGLIDLETALLYARRPADLELRLKGISTDEDIIEGDNYLT

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=332, Percent_Identity=37.9518072289157, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1786296, Length=285, Percent_Identity=35.0877192982456, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789723, Length=287, Percent_Identity=33.4494773519164, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41998; Mature: 41998

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQILRKALEKKASDIHLKVGSKPVIRTPDGLDTLEEFPPIDIDLFEVFVKEILEHHPK
CHHHHHHHHHHCCCCCEEEEECCCCCEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCC
KKKELEEQGQVDLSYAIPKVSRFRVNLYKQRGTYAMALRVIPFDIPEFKKLNLPPVMLQT
HHHHHHHHCCEEEEHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHHHHH
ALKHSAGMILVTGPTGSGKSTTIASIINEINKRLSRVIITIEDPIEYLFKDIQSFIAQRE
HHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHH
VGWDTESFFLGLRAALREDPDIIFVGEIRDTETAKTALHAAETGHLVFSTLHTLDAVETI
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
NRFIGMFPLEHRDQIRQMLASTLIAVYSQRLVSKKNGGGKVPVVEIMIMTQTIREAILEN
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
RPQDIPELIEKGRLVYGSQTFDQHLEELYRKGLIDLETALLYARRPADLELRLKGISTDE
CCCHHHHHHHCCCEEECCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCH
DIIEGDNYLT
HHHCCCCCCC
>Mature Secondary Structure
MLDQILRKALEKKASDIHLKVGSKPVIRTPDGLDTLEEFPPIDIDLFEVFVKEILEHHPK
CHHHHHHHHHHCCCCCEEEEECCCCCEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCC
KKKELEEQGQVDLSYAIPKVSRFRVNLYKQRGTYAMALRVIPFDIPEFKKLNLPPVMLQT
HHHHHHHHCCEEEEHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHHHHH
ALKHSAGMILVTGPTGSGKSTTIASIINEINKRLSRVIITIEDPIEYLFKDIQSFIAQRE
HHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHH
VGWDTESFFLGLRAALREDPDIIFVGEIRDTETAKTALHAAETGHLVFSTLHTLDAVETI
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
NRFIGMFPLEHRDQIRQMLASTLIAVYSQRLVSKKNGGGKVPVVEIMIMTQTIREAILEN
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
RPQDIPELIEKGRLVYGSQTFDQHLEELYRKGLIDLETALLYARRPADLELRLKGISTDE
CCCHHHHHHHCCCEEECCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCH
DIIEGDNYLT
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]