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The map label for this gene is ppsA

Identifier: 15607086

GI number: 15607086

Start: 1508458

End: 1511028

Strand: Direct

Name: ppsA

Synonym: aq_2142

Alternate gene names: 15607086

Gene position: 1508458-1511028 (Clockwise)

Preceding gene: 15607085

Following gene: 15607087

Centisome position: 97.24

GC content: 46.13

Gene sequence:

>2571_bases
ATGTCCGTTGATAAAAGCAAAGCTCTTGTTCTTTGGCTCGATGAAGTTACCATTGAAGATATCCCTATAGCTGGCGGTAA
GAACGCATCGTTAGGTGAGATGATAAGAAATCTCTCACCTCTTGGCGTGAAAATTCCCTATGGATACGTCGTTACCGCAA
ACGCTTACTACTACTTCCTCGATTACAACAACCTGAGGGACAAAATAAGGAAGATTCTGGAGGGGTTGAACACGGACGAC
CTGAAGGACCTTCAGAGGAGGGGACACGAGGTAAGGGAACTTATAAGAGGGGGAACGTTTCCACCGGATTTAGAAGAAGC
TATAAAGGATTATTATAATAAGCTATCGGAAAAGTACAAAACCCACGCCGTTGACGTTGCTGTTCGTTCTTCTGCAACCG
CTGAGGACCTTCCGGACGCTTCCTTTGCAGGACAGCAGGAAACTTACCTGAACGTTGTTGGTGCGGAAAATGTCCTCGTA
GCTATAAAGAACTGCTTTGCATCCCTCTTTACGGACAGGGCCATCGTTTACAGGGAAAGATTCGGGTTTGACCACTTTAA
GGTAGGTATAGCGGTAGGCGTTCAGAAGATGGTTCGCTCCGATATGGGAGCTTCAGGTGTTATGTTTACCCTTGATACGG
AAACAGGTTTTAAAGACGTAGTAGTAATAAACGCAGCCTACGGACTCGGTGAACTCCTCGTGAGGGGAGAAGTCACCCCG
GACGAGTACATAGTGTTCAAACCCACCCTAATGAAGGGATACTCAGCGATAATAGAAAAGAAACTAGGGAGAAAAGACAG
GAAGATGATTTACGGAACGGGGGACGAGAGGGTAAAGATAGTAAACGTTCCCAAGGAAGACCAGAAAAAGTTTGCACTAA
ACGACGACGAAATTCTACAGCTCGCAAAGTGGGGCGTCCTTATAGAAGAGCACTACTCCAAAAAGAACGGTAGATGGACA
CCTATGGATATTGAGTGGGCAAAAGACGGGATACTCAACGAGCTTTTTGTGGTTCAGGCGAGACCCGAAACTGTTCACTC
CAGAAAGAAGGAAAACGTAGTGAAGATTTACAAGATAAAAACCCCTGAAGAGGAAAGGAAGAACAGGGTAATAGTAAAGG
GGATAGCCGTAGGAGACAAGATAGCAACCGGAAAGGCGAGGGTTCTTTTTGACTTAAAAGAGGCGGACCAGTTCCAGGAA
GGTGAGATACTCGTCACGGACATCACGGACCCGGACTGGGAACCCGTAATGAAGAAAGCTGCGGCTATAGTAACTAACAG
AGGAGGAAGGACTTCTCACGCGGCGATAGTCGCAAGGGAACTGGGAATTCCTGCAGTTGTGGGAACGGGAAACGCAACCG
AAAAGATAAAAACGGGAGAAGAAATAACCGTATCCTGCGCAGAAGGTGAAACGGGATACGTCTACGAAGGTAAGATAGAC
TATGAAGTAGAAGAGATAAACCTTGAAAACATTCCAAAGCCAAAAACAAAGATAATGATGAACATAGGAAATCCCGAGTC
CGCCTTCAGGTACGCATCACTGCCAAACGACGGGGTGGGATTGGCAAGGGAAGAATTCATAATAGCAAACTACATAAAGA
TACACCCCCTTGCACTGCTCCACTACGAGGACCTCAAAGAACTCTACGAGAAACTGGAGAGGGAAAACCTAATAGACGAA
AAAGGCTTTGTCCAGTTTAAACTCATTTACCACTACGCAAACGGAAGGCTTGCAAATAAACTCGCAAAGGGCAAAGACAA
ACTCAGGGTTAACTTGAGGAAGATACTTCAGGACATAGAAAACCTGACCTTCGGTTACGAGGACAAGGCGACCTACTACA
TAAAGAAACTCTCTTACGGTATAGCGAAAATAGCGGCTGCTTTTTACCCAAATCCTGTAATAGTGAGATTTTCCGACTTC
AAGTCTAACGAGTACGCAAACCTTATCGGCGGAATTCTCTTTGAACCTGAAGAAGAGAACCCAATGCTCGGTTGGAGGGG
AGCGTCAAGGTATTACTCTGACGTCTTTAAGGAAGCCTTCGGAATGGAGTGCAAGGCGATTATCAGAGTTAGGAACAAGA
TGGGACTCACCAACACAAAAGTTATGATACCCTTCTGCAGGACACCCGAGGAAGGAGAAAAGGTCCTTCAGGTAATGGAA
GAGTATGGACTCAGAAAAGGGGAAAATGGACTGGAAGTTTACGTTATGGCAGAACTTCCCAGCAACATTGTCCTTGCTGA
CAGATACGCTCAGATATTTGACGGTTTCTCTATAGGTTCTAACGACCTCACTCAGCTCACTTTGGGACTGGACAGGGATT
CTGAACTCGTGGCACACCTCTACGACGAAAGGAACGAGGCTGTAAAAAGGCTCATCGCTCAACTCATAAAAACAGCAAAG
GAGTACGGCAGGAAAGTGGGAATATGCGGTCAGGCTCCTTCGGACTTTCCGGAATTCGCTCAGTTTCTCGTTGAACAGGG
AATAGACAGTATATCCCTCAACCCAGACTCCGTACTCAAGACCATGCTCGCGGTAGTAGAAATGGAGAAAAAACTCGGAG
TCCTTAAATGA

Upstream 100 bases:

>100_bases
GAAAAAATAAGAAACAACAGGATATCCCTCTTAAAGAGAGTAGAAAACCTCTTTAGAACGTTCGGAGACTTTAACGAAAT
TGTTATAAAGGAGGGCTGAT

Downstream 100 bases:

>100_bases
AACTCCTAGAGAAGATAAAGGAAAGGACAAAAGGTTTCCGTTTACCTTCCCTTTCTCTCTCCCCTAAAACTTCACTTTCC
GTTTTCCTAACTCCTGAATT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase

Number of amino acids: Translated: 856; Mature: 855

Protein sequence:

>856_residues
MSVDKSKALVLWLDEVTIEDIPIAGGKNASLGEMIRNLSPLGVKIPYGYVVTANAYYYFLDYNNLRDKIRKILEGLNTDD
LKDLQRRGHEVRELIRGGTFPPDLEEAIKDYYNKLSEKYKTHAVDVAVRSSATAEDLPDASFAGQQETYLNVVGAENVLV
AIKNCFASLFTDRAIVYRERFGFDHFKVGIAVGVQKMVRSDMGASGVMFTLDTETGFKDVVVINAAYGLGELLVRGEVTP
DEYIVFKPTLMKGYSAIIEKKLGRKDRKMIYGTGDERVKIVNVPKEDQKKFALNDDEILQLAKWGVLIEEHYSKKNGRWT
PMDIEWAKDGILNELFVVQARPETVHSRKKENVVKIYKIKTPEEERKNRVIVKGIAVGDKIATGKARVLFDLKEADQFQE
GEILVTDITDPDWEPVMKKAAAIVTNRGGRTSHAAIVARELGIPAVVGTGNATEKIKTGEEITVSCAEGETGYVYEGKID
YEVEEINLENIPKPKTKIMMNIGNPESAFRYASLPNDGVGLAREEFIIANYIKIHPLALLHYEDLKELYEKLERENLIDE
KGFVQFKLIYHYANGRLANKLAKGKDKLRVNLRKILQDIENLTFGYEDKATYYIKKLSYGIAKIAAAFYPNPVIVRFSDF
KSNEYANLIGGILFEPEEENPMLGWRGASRYYSDVFKEAFGMECKAIIRVRNKMGLTNTKVMIPFCRTPEEGEKVLQVME
EYGLRKGENGLEVYVMAELPSNIVLADRYAQIFDGFSIGSNDLTQLTLGLDRDSELVAHLYDERNEAVKRLIAQLIKTAK
EYGRKVGICGQAPSDFPEFAQFLVEQGIDSISLNPDSVLKTMLAVVEMEKKLGVLK

Sequences:

>Translated_856_residues
MSVDKSKALVLWLDEVTIEDIPIAGGKNASLGEMIRNLSPLGVKIPYGYVVTANAYYYFLDYNNLRDKIRKILEGLNTDD
LKDLQRRGHEVRELIRGGTFPPDLEEAIKDYYNKLSEKYKTHAVDVAVRSSATAEDLPDASFAGQQETYLNVVGAENVLV
AIKNCFASLFTDRAIVYRERFGFDHFKVGIAVGVQKMVRSDMGASGVMFTLDTETGFKDVVVINAAYGLGELLVRGEVTP
DEYIVFKPTLMKGYSAIIEKKLGRKDRKMIYGTGDERVKIVNVPKEDQKKFALNDDEILQLAKWGVLIEEHYSKKNGRWT
PMDIEWAKDGILNELFVVQARPETVHSRKKENVVKIYKIKTPEEERKNRVIVKGIAVGDKIATGKARVLFDLKEADQFQE
GEILVTDITDPDWEPVMKKAAAIVTNRGGRTSHAAIVARELGIPAVVGTGNATEKIKTGEEITVSCAEGETGYVYEGKID
YEVEEINLENIPKPKTKIMMNIGNPESAFRYASLPNDGVGLAREEFIIANYIKIHPLALLHYEDLKELYEKLERENLIDE
KGFVQFKLIYHYANGRLANKLAKGKDKLRVNLRKILQDIENLTFGYEDKATYYIKKLSYGIAKIAAAFYPNPVIVRFSDF
KSNEYANLIGGILFEPEEENPMLGWRGASRYYSDVFKEAFGMECKAIIRVRNKMGLTNTKVMIPFCRTPEEGEKVLQVME
EYGLRKGENGLEVYVMAELPSNIVLADRYAQIFDGFSIGSNDLTQLTLGLDRDSELVAHLYDERNEAVKRLIAQLIKTAK
EYGRKVGICGQAPSDFPEFAQFLVEQGIDSISLNPDSVLKTMLAVVEMEKKLGVLK
>Mature_855_residues
SVDKSKALVLWLDEVTIEDIPIAGGKNASLGEMIRNLSPLGVKIPYGYVVTANAYYYFLDYNNLRDKIRKILEGLNTDDL
KDLQRRGHEVRELIRGGTFPPDLEEAIKDYYNKLSEKYKTHAVDVAVRSSATAEDLPDASFAGQQETYLNVVGAENVLVA
IKNCFASLFTDRAIVYRERFGFDHFKVGIAVGVQKMVRSDMGASGVMFTLDTETGFKDVVVINAAYGLGELLVRGEVTPD
EYIVFKPTLMKGYSAIIEKKLGRKDRKMIYGTGDERVKIVNVPKEDQKKFALNDDEILQLAKWGVLIEEHYSKKNGRWTP
MDIEWAKDGILNELFVVQARPETVHSRKKENVVKIYKIKTPEEERKNRVIVKGIAVGDKIATGKARVLFDLKEADQFQEG
EILVTDITDPDWEPVMKKAAAIVTNRGGRTSHAAIVARELGIPAVVGTGNATEKIKTGEEITVSCAEGETGYVYEGKIDY
EVEEINLENIPKPKTKIMMNIGNPESAFRYASLPNDGVGLAREEFIIANYIKIHPLALLHYEDLKELYEKLERENLIDEK
GFVQFKLIYHYANGRLANKLAKGKDKLRVNLRKILQDIENLTFGYEDKATYYIKKLSYGIAKIAAAFYPNPVIVRFSDFK
SNEYANLIGGILFEPEEENPMLGWRGASRYYSDVFKEAFGMECKAIIRVRNKMGLTNTKVMIPFCRTPEEGEKVLQVMEE
YGLRKGENGLEVYVMAELPSNIVLADRYAQIFDGFSIGSNDLTQLTLGLDRDSELVAHLYDERNEAVKRLIAQLIKTAKE
YGRKVGICGQAPSDFPEFAQFLVEQGIDSISLNPDSVLKTMLAVVEMEKKLGVLK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1787994, Length=853, Percent_Identity=49.5896834701055, Blast_Score=861, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=224, Percent_Identity=31.6964285714286, Blast_Score=99, Evalue=2e-21,
Organism=Escherichia coli, GI1788726, Length=190, Percent_Identity=33.6842105263158, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1789193, Length=221, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI48994992, Length=191, Percent_Identity=31.413612565445, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17564524, Length=237, Percent_Identity=32.0675105485232, Blast_Score=85, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPSA_AQUAE (O67899)

Other databases:

- EMBL:   AE000657
- PIR:   G70483
- RefSeq:   NP_214468.1
- ProteinModelPortal:   O67899
- SMR:   O67899
- GeneID:   1193888
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_2142
- NMPDR:   fig|224324.1.peg.1483
- HOGENOM:   HBG284863
- OMA:   ALIHFDE
- PhylomeDB:   O67899
- ProtClustDB:   PRK06464
- BioCyc:   AAEO224324:AQ_2142-MONOMER
- BRENDA:   2.7.9.2
- GO:   GO:0006094
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:3.20.20.60
- PRINTS:   PR01736
- TIGRFAMs:   TIGR01418

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N; SSF52009 PEP_mobile; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.9.2

Molecular weight: Translated: 96408; Mature: 96277

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 433-433 ACT_SITE 809-809 BINDING 523-523 BINDING 636-636 BINDING 738-738 BINDING 759-759 BINDING 760-760 BINDING 761-761 BINDING 762-762

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDKSKALVLWLDEVTIEDIPIAGGKNASLGEMIRNLSPLGVKIPYGYVVTANAYYYFL
CCCCCCCEEEEEEECCEEECCCCCCCCCCCHHHHHHCCCCCCEECCCCEEEEECEEEEEE
DYNNLRDKIRKILEGLNTDDLKDLQRRGHEVRELIRGGTFPPDLEEAIKDYYNKLSEKYK
ECCHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
THAVDVAVRSSATAEDLPDASFAGQQETYLNVVGAENVLVAIKNCFASLFTDRAIVYRER
HHHEEEEECCCCCHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHH
FGFDHFKVGIAVGVQKMVRSDMGASGVMFTLDTETGFKDVVVINAAYGLGELLVRGEVTP
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHEECCCCC
DEYIVFKPTLMKGYSAIIEKKLGRKDRKMIYGTGDERVKIVNVPKEDQKKFALNDDEILQ
CCEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCHHCCCCCCCHHHHHH
LAKWGVLIEEHYSKKNGRWTPMDIEWAKDGILNELFVVQARPETVHSRKKENVVKIYKIK
HHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEECCCHHHHHHCCCCEEEEEEEC
TPEEERKNRVIVKGIAVGDKIATGKARVLFDLKEADQFQEGEILVTDITDPDWEPVMKKA
CCHHHHCCEEEEEEEEECCEECCCCEEEEEEEHHCCCCCCCCEEEEECCCCCHHHHHHHH
AAIVTNRGGRTSHAAIVARELGIPAVVGTGNATEKIKTGEEITVSCAEGETGYVYEGKID
HHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHCCCCEEEEEEECCCCCEEEECEEC
YEVEEINLENIPKPKTKIMMNIGNPESAFRYASLPNDGVGLAREEFIIANYIKIHPLALL
EEEEEECCCCCCCCCCEEEEECCCCHHHEEECCCCCCCCCCCHHHEEEECHHEECEEEEE
HYEDLKELYEKLERENLIDEKGFVQFKLIYHYANGRLANKLAKGKDKLRVNLRKILQDIE
EHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHH
NLTFGYEDKATYYIKKLSYGIAKIAAAFYPNPVIVRFSDFKSNEYANLIGGILFEPEEEN
HCCCCCCCCHHEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCEEECCCCCC
PMLGWRGASRYYSDVFKEAFGMECKAIIRVRNKMGLTNTKVMIPFCRTPEEGEKVLQVME
CCEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHH
EYGLRKGENGLEVYVMAELPSNIVLADRYAQIFDGFSIGSNDLTQLTLGLDRDSELVAHL
HHCCCCCCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHH
YDERNEAVKRLIAQLIKTAKEYGRKVGICGQAPSDFPEFAQFLVEQGIDSISLNPDSVLK
HHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
TMLAVVEMEKKLGVLK
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SVDKSKALVLWLDEVTIEDIPIAGGKNASLGEMIRNLSPLGVKIPYGYVVTANAYYYFL
CCCCCCEEEEEEECCEEECCCCCCCCCCCHHHHHHCCCCCCEECCCCEEEEECEEEEEE
DYNNLRDKIRKILEGLNTDDLKDLQRRGHEVRELIRGGTFPPDLEEAIKDYYNKLSEKYK
ECCHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
THAVDVAVRSSATAEDLPDASFAGQQETYLNVVGAENVLVAIKNCFASLFTDRAIVYRER
HHHEEEEECCCCCHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHH
FGFDHFKVGIAVGVQKMVRSDMGASGVMFTLDTETGFKDVVVINAAYGLGELLVRGEVTP
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHEECCCCC
DEYIVFKPTLMKGYSAIIEKKLGRKDRKMIYGTGDERVKIVNVPKEDQKKFALNDDEILQ
CCEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCHHCCCCCCCHHHHHH
LAKWGVLIEEHYSKKNGRWTPMDIEWAKDGILNELFVVQARPETVHSRKKENVVKIYKIK
HHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEECCCHHHHHHCCCCEEEEEEEC
TPEEERKNRVIVKGIAVGDKIATGKARVLFDLKEADQFQEGEILVTDITDPDWEPVMKKA
CCHHHHCCEEEEEEEEECCEECCCCEEEEEEEHHCCCCCCCCEEEEECCCCCHHHHHHHH
AAIVTNRGGRTSHAAIVARELGIPAVVGTGNATEKIKTGEEITVSCAEGETGYVYEGKID
HHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHCCCCEEEEEEECCCCCEEEECEEC
YEVEEINLENIPKPKTKIMMNIGNPESAFRYASLPNDGVGLAREEFIIANYIKIHPLALL
EEEEEECCCCCCCCCCEEEEECCCCHHHEEECCCCCCCCCCCHHHEEEECHHEECEEEEE
HYEDLKELYEKLERENLIDEKGFVQFKLIYHYANGRLANKLAKGKDKLRVNLRKILQDIE
EHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHH
NLTFGYEDKATYYIKKLSYGIAKIAAAFYPNPVIVRFSDFKSNEYANLIGGILFEPEEEN
HCCCCCCCCHHEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCEEECCCCCC
PMLGWRGASRYYSDVFKEAFGMECKAIIRVRNKMGLTNTKVMIPFCRTPEEGEKVLQVME
CCEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHH
EYGLRKGENGLEVYVMAELPSNIVLADRYAQIFDGFSIGSNDLTQLTLGLDRDSELVAHL
HHCCCCCCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHH
YDERNEAVKRLIAQLIKTAKEYGRKVGICGQAPSDFPEFAQFLVEQGIDSISLNPDSVLK
HHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
TMLAVVEMEKKLGVLK
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320