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The map label for this gene is glyS

Identifier: 15607085

GI number: 15607085

Start: 1506462

End: 1508456

Strand: Direct

Name: glyS

Synonym: aq_2141

Alternate gene names: 15607085

Gene position: 1506462-1508456 (Clockwise)

Preceding gene: 15607083

Following gene: 15607086

Centisome position: 97.11

GC content: 42.86

Gene sequence:

>1995_bases
ATGACCAACGAGCTTCTTATCGAAATAGGCACGGAGGAGCTTCCCGCCGGAGTAATAAACCCAGCACTTGATTACTTAAA
AGATAAGATAAACTCCCTTCTCAACGCAAGACAAGTAAAAACCTATGGAACACCCAGGAGACTAACCCTTTACTTTAAAG
ACTTTGAAAATGAAAGAAAGGAAAAAAAAGAGGTAATATGGGGTCCTCCCAAAAATGTAGCTTACGACGAGAAGGGAAAT
CCCACAAAAGCCCTTGAAGGTTTCCTTAAAAAAAACAACGCATCACTTGAAGAGGTAAAAGTTTTAAAGAAAGACAAAGG
CGAGTACGTGGCAATAGTAAGAAAAGTAATAGAAAAATCACCAATTGAGAAACTTCAGGAAGAGTTTGAAGAAATCCTTC
TTTCCGTTCCCTTCCCAAAACGAATGCGCTGGACCTCTTCAAAGAGGATAACCTTCTCAAGACCTGTCAGGTGGATTCTC
GCCCTCTTCAACGGCCAAGTGCTAAAACTGAGGTTCGGAGAACTGGAGAGTTCCAATAAAACCTACGGCCACAGGTTCTT
ATCAAAAGGCGAAGTAACGATAAACAACCCAGCAGATTACGAAAAAACCTTGAAAGAACACTACGTTATACCGGACTTCA
ACGAAAGGAAGGAGATTATTCTCAGGGCCCTTGAAAAGAGCTCTCAGGAAGTAGGAGGAAAACCCTCTTACCCTGAGGGA
CTCGTTGAAGAAGTTACGAACCTCGTGGAATACCCCTTTCCCGTCCTCGGGAAGTTTGACGAAAAGTACTTAGAACTCCC
TCCCCTCGTCATCACAACCGTGGCGGCTCACCATCAGAGGTTTTTCTGCTTTGAAAAAGACGGAAAACTCTTAAACTACT
TCCTCGGAATTAGCAATAACAAGCCAAATGAGAAGATAAAGGAAGGATATGAAAAAGTTCTGAGAGCGAGGCTGGAGGAT
GCACTCTTCTTTTACAGGGAAGATTTAAAAAAGGACCTGAAGAGTTTAATCCCCGAACTCAAAAAGGTACTCTTCCACCC
TAAAGTCGGCTCTATGTACGAGAAAGAGGAGAGGATGGAAAAAATAGCCCAGAAGCTCTGTCCACTCCTTAAGTGTGAAT
GGGAAAAGGTAAAGGAAGCGGTCTGGCTCTCAAAGGTTGACCTTTTAACGGAGATGGTAAAGGAACTCGACGAACTTCAG
GGCTACATGGGATACGTTTACGCAAAAGCTCAGGGCTACGACGAAGAAGTTGCAAAAGCCCTCTGGGAGCAGTACTTTCC
AAGGAGCTTGGAAGACAAAGTTCCTGAGACCACAACTGGCACAATACTCTCCCTTTCAGACAAGATAGACAACCTATACT
CCTTCTTTAAAGCGGGAGAAATCCCCAAGGGAAGTTCGGACCCTTACGGACTCAGGAGAAGTGCCTTCGGAATTATAAAG
ATCGTGGATGTGAAAAATCTGGACCTCAATCTAGAAGATTTCAAGGAAATATACGGGGAATTTAAGCAATATCCAAAACT
CGTTGAATTCCTGAAACAGAGGTTAATCTCCTACCTTGAGGACTATCCCGTGGATATAGTAAGAGCTGTCCTGAACGTTT
ACTCTCCCATGGAGCCTTACAAAGTGATAAACAGCGTCAGAGTACTTTACGAAGCTTCAAAATCCCCTGAATTTCCCTCG
GTCGTTGAGGCTGCAAAGCGTGTTATAAGGATAATTCCGAAGGACTGGAAAAATTACGAGGTTGACGAGAAATTATTAAG
TGAGGAGGCTGAGAGGGAGTTGTACCAGAAACTTACAGAGTTTGAAAATAAAGAACTCAAAAGTCCTCTTGAACTGCTTC
CCTTGAAAGAGTACATAGACAAGTTCTTTGACAACGTAAAGGTAATGGCCGAAGACGAAAAAATAAGAAACAACAGGATA
TCCCTCTTAAAGAGAGTAGAAAACCTCTTTAGAACGTTCGGAGACTTTAACGAAATTGTTATAAAGGAGGGCTGA

Upstream 100 bases:

>100_bases
CTCAAAACCCTGTGAACTCAAAAGAGACCTAACTCTTGAGGCCAGTATAAGGTTCGTGTCGTAGAGAAAGACCTTCATAC
TATGTTAAAATTTTAGGCAA

Downstream 100 bases:

>100_bases
TATGTCCGTTGATAAAAGCAAAGCTCTTGTTCTTTGGCTCGATGAAGTTACCATTGAAGATATCCCTATAGCTGGCGGTA
AGAACGCATCGTTAGGTGAG

Product: glycyl-tRNA synthetase beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MTNELLIEIGTEELPAGVINPALDYLKDKINSLLNARQVKTYGTPRRLTLYFKDFENERKEKKEVIWGPPKNVAYDEKGN
PTKALEGFLKKNNASLEEVKVLKKDKGEYVAIVRKVIEKSPIEKLQEEFEEILLSVPFPKRMRWTSSKRITFSRPVRWIL
ALFNGQVLKLRFGELESSNKTYGHRFLSKGEVTINNPADYEKTLKEHYVIPDFNERKEIILRALEKSSQEVGGKPSYPEG
LVEEVTNLVEYPFPVLGKFDEKYLELPPLVITTVAAHHQRFFCFEKDGKLLNYFLGISNNKPNEKIKEGYEKVLRARLED
ALFFYREDLKKDLKSLIPELKKVLFHPKVGSMYEKEERMEKIAQKLCPLLKCEWEKVKEAVWLSKVDLLTEMVKELDELQ
GYMGYVYAKAQGYDEEVAKALWEQYFPRSLEDKVPETTTGTILSLSDKIDNLYSFFKAGEIPKGSSDPYGLRRSAFGIIK
IVDVKNLDLNLEDFKEIYGEFKQYPKLVEFLKQRLISYLEDYPVDIVRAVLNVYSPMEPYKVINSVRVLYEASKSPEFPS
VVEAAKRVIRIIPKDWKNYEVDEKLLSEEAERELYQKLTEFENKELKSPLELLPLKEYIDKFFDNVKVMAEDEKIRNNRI
SLLKRVENLFRTFGDFNEIVIKEG

Sequences:

>Translated_664_residues
MTNELLIEIGTEELPAGVINPALDYLKDKINSLLNARQVKTYGTPRRLTLYFKDFENERKEKKEVIWGPPKNVAYDEKGN
PTKALEGFLKKNNASLEEVKVLKKDKGEYVAIVRKVIEKSPIEKLQEEFEEILLSVPFPKRMRWTSSKRITFSRPVRWIL
ALFNGQVLKLRFGELESSNKTYGHRFLSKGEVTINNPADYEKTLKEHYVIPDFNERKEIILRALEKSSQEVGGKPSYPEG
LVEEVTNLVEYPFPVLGKFDEKYLELPPLVITTVAAHHQRFFCFEKDGKLLNYFLGISNNKPNEKIKEGYEKVLRARLED
ALFFYREDLKKDLKSLIPELKKVLFHPKVGSMYEKEERMEKIAQKLCPLLKCEWEKVKEAVWLSKVDLLTEMVKELDELQ
GYMGYVYAKAQGYDEEVAKALWEQYFPRSLEDKVPETTTGTILSLSDKIDNLYSFFKAGEIPKGSSDPYGLRRSAFGIIK
IVDVKNLDLNLEDFKEIYGEFKQYPKLVEFLKQRLISYLEDYPVDIVRAVLNVYSPMEPYKVINSVRVLYEASKSPEFPS
VVEAAKRVIRIIPKDWKNYEVDEKLLSEEAERELYQKLTEFENKELKSPLELLPLKEYIDKFFDNVKVMAEDEKIRNNRI
SLLKRVENLFRTFGDFNEIVIKEG
>Mature_663_residues
TNELLIEIGTEELPAGVINPALDYLKDKINSLLNARQVKTYGTPRRLTLYFKDFENERKEKKEVIWGPPKNVAYDEKGNP
TKALEGFLKKNNASLEEVKVLKKDKGEYVAIVRKVIEKSPIEKLQEEFEEILLSVPFPKRMRWTSSKRITFSRPVRWILA
LFNGQVLKLRFGELESSNKTYGHRFLSKGEVTINNPADYEKTLKEHYVIPDFNERKEIILRALEKSSQEVGGKPSYPEGL
VEEVTNLVEYPFPVLGKFDEKYLELPPLVITTVAAHHQRFFCFEKDGKLLNYFLGISNNKPNEKIKEGYEKVLRARLEDA
LFFYREDLKKDLKSLIPELKKVLFHPKVGSMYEKEERMEKIAQKLCPLLKCEWEKVKEAVWLSKVDLLTEMVKELDELQG
YMGYVYAKAQGYDEEVAKALWEQYFPRSLEDKVPETTTGTILSLSDKIDNLYSFFKAGEIPKGSSDPYGLRRSAFGIIKI
VDVKNLDLNLEDFKEIYGEFKQYPKLVEFLKQRLISYLEDYPVDIVRAVLNVYSPMEPYKVINSVRVLYEASKSPEFPSV
VEAAKRVIRIIPKDWKNYEVDEKLLSEEAERELYQKLTEFENKELKSPLELLPLKEYIDKFFDNVKVMAEDEKIRNNRIS
LLKRVENLFRTFGDFNEIVIKEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=683, Percent_Identity=32.0644216691069, Blast_Score=334, Evalue=9e-93,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_AQUAE (O67898)

Other databases:

- EMBL:   AE000657
- PIR:   F70483
- RefSeq:   NP_214467.1
- ProteinModelPortal:   O67898
- GeneID:   1193887
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_2141
- NMPDR:   fig|224324.1.peg.1482
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   O67898
- ProtClustDB:   CLSK2748563
- BioCyc:   AAEO224324:AQ_2141-MONOMER
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 77524; Mature: 77393

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNELLIEIGTEELPAGVINPALDYLKDKINSLLNARQVKTYGTPRRLTLYFKDFENERK
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHH
EKKEVIWGPPKNVAYDEKGNPTKALEGFLKKNNASLEEVKVLKKDKGEYVAIVRKVIEKS
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHC
PIEKLQEEFEEILLSVPFPKRMRWTSSKRITFSRPVRWILALFNGQVLKLRFGELESSNK
HHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEHHHHHHHHHHHHCCCEEEEEECCCCCCCC
TYGHRFLSKGEVTINNPADYEKTLKEHYVIPDFNERKEIILRALEKSSQEVGGKPSYPEG
HHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHH
LVEEVTNLVEYPFPVLGKFDEKYLELPPLVITTVAAHHQRFFCFEKDGKLLNYFLGISNN
HHHHHHHHHHCCCHHHCCCCHHHHCCCCHHHHHHHHHHHHEEEECCCCCHHHHHHCCCCC
KPNEKIKEGYEKVLRARLEDALFFYREDLKKDLKSLIPELKKVLFHPKVGSMYEKEERME
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KIAQKLCPLLKCEWEKVKEAVWLSKVDLLTEMVKELDELQGYMGYVYAKAQGYDEEVAKA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHH
LWEQYFPRSLEDKVPETTTGTILSLSDKIDNLYSFFKAGEIPKGSSDPYGLRRSAFGIIK
HHHHHCCCCHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCEEE
IVDVKNLDLNLEDFKEIYGEFKQYPKLVEFLKQRLISYLEDYPVDIVRAVLNVYSPMEPY
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
KVINSVRVLYEASKSPEFPSVVEAAKRVIRIIPKDWKNYEVDEKLLSEEAERELYQKLTE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FENKELKSPLELLPLKEYIDKFFDNVKVMAEDEKIRNNRISLLKRVENLFRTFGDFNEIV
HCCHHHCCHHHHCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IKEG
EECC
>Mature Secondary Structure 
TNELLIEIGTEELPAGVINPALDYLKDKINSLLNARQVKTYGTPRRLTLYFKDFENERK
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHH
EKKEVIWGPPKNVAYDEKGNPTKALEGFLKKNNASLEEVKVLKKDKGEYVAIVRKVIEKS
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHC
PIEKLQEEFEEILLSVPFPKRMRWTSSKRITFSRPVRWILALFNGQVLKLRFGELESSNK
HHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEHHHHHHHHHHHHCCCEEEEEECCCCCCCC
TYGHRFLSKGEVTINNPADYEKTLKEHYVIPDFNERKEIILRALEKSSQEVGGKPSYPEG
HHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHH
LVEEVTNLVEYPFPVLGKFDEKYLELPPLVITTVAAHHQRFFCFEKDGKLLNYFLGISNN
HHHHHHHHHHCCCHHHCCCCHHHHCCCCHHHHHHHHHHHHEEEECCCCCHHHHHHCCCCC
KPNEKIKEGYEKVLRARLEDALFFYREDLKKDLKSLIPELKKVLFHPKVGSMYEKEERME
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KIAQKLCPLLKCEWEKVKEAVWLSKVDLLTEMVKELDELQGYMGYVYAKAQGYDEEVAKA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHH
LWEQYFPRSLEDKVPETTTGTILSLSDKIDNLYSFFKAGEIPKGSSDPYGLRRSAFGIIK
HHHHHCCCCHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCEEE
IVDVKNLDLNLEDFKEIYGEFKQYPKLVEFLKQRLISYLEDYPVDIVRAVLNVYSPMEPY
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
KVINSVRVLYEASKSPEFPSVVEAAKRVIRIIPKDWKNYEVDEKLLSEEAERELYQKLTE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FENKELKSPLELLPLKEYIDKFFDNVKVMAEDEKIRNNRISLLKRVENLFRTFGDFNEIV
HCCHHHCCHHHHCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IKEG
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320