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The map label for this gene is rph

Identifier: 15603741

GI number: 15603741

Start: 2118856

End: 2119572

Strand: Direct

Name: rph

Synonym: PM1876

Alternate gene names: 15603741

Gene position: 2118856-2119572 (Clockwise)

Preceding gene: 15603738

Following gene: 15603742

Centisome position: 93.86

GC content: 46.58

Gene sequence:

>717_bases
ATGCGTCCAAATGAACGTGCAAATAATCAGCCCCGCCCGATCAAAATTACTCGTCATTATACAAAACATGCGGAAGGCTC
AGTGTTGGTTGAGTTTGGAGACACGAAAGTACTCTGTACAGCGACGGTGGAAGAGTCGGTGCCTCGCTTTTTGAAAGGTC
AAAATCAAGGATGGGTAACGGCGGAATATGGGATGTTGCCACGTTCAACCCATAGCCGTATGCAACGTGAGGCAGCAAAA
GGTAAACAAGGCGGACGCACACTTGAAATTCAGCGTTTAATTGCGCGCTCATTGCGGGCAATGGTAGATTTAGAAGCCTT
AGGCGAACGCTCGATTACATTGGATTGTGATGTGATTCAAGCAGATGGGGGAACACGCACAGCATCGATTACTGGCGCTT
GCGTAGCCTTATGTGATGCGATTCATGGTTTAGTGGAAAATGGCACCTTAAAAGTAAATCCTATTAAAAGTTTAGTGGCG
GCGATTTCAGTAGGGATTGTTGAGGGCGTGCCGGTTTGTGATCTGGAATATGTCGAAGATTCTGCGGCAGAAACGGATAT
GAATGTCGTGATGATGGAAGATGGGAGAATGATTGAAGTACAGGGAACAGCAGAAGGGGAACCATTCAGTCATGAAGAAT
TATTGACCTTGTTAGCCTTAGCCAAAGAAGGCTGTCAGCTTATTTTCGATGTGCAACGTCAGGCGCTAGCCAAGTAG

Upstream 100 bases:

>100_bases
TAACAGAACTATTGTACTGGTAAATTTCGTTTCTGTCAGTTTGCGTGCTAAAATACTGCGAATTTTTTTATCATGAAGAA
AGAACAATAAGGAATAAACT

Downstream 100 bases:

>100_bases
GAAGGAGAGCGGATCATGGAAGCCTATAAATCGGAATTTATTCAATTTGCGCTAAGCCGTCAGGTGTTAAAATTTGGTGA
GTTTACTCTAAAATCTGGTC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPNERANNQPRPIKITRHYTKHAEGSVLVEFGDTKVLCTATVEESVPRFLKGQNQGWVTAEYGMLPRSTHSRMQREAAK
GKQGGRTLEIQRLIARSLRAMVDLEALGERSITLDCDVIQADGGTRTASITGACVALCDAIHGLVENGTLKVNPIKSLVA
AISVGIVEGVPVCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLALAKEGCQLIFDVQRQALAK

Sequences:

>Translated_238_residues
MRPNERANNQPRPIKITRHYTKHAEGSVLVEFGDTKVLCTATVEESVPRFLKGQNQGWVTAEYGMLPRSTHSRMQREAAK
GKQGGRTLEIQRLIARSLRAMVDLEALGERSITLDCDVIQADGGTRTASITGACVALCDAIHGLVENGTLKVNPIKSLVA
AISVGIVEGVPVCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLALAKEGCQLIFDVQRQALAK
>Mature_238_residues
MRPNERANNQPRPIKITRHYTKHAEGSVLVEFGDTKVLCTATVEESVPRFLKGQNQGWVTAEYGMLPRSTHSRMQREAAK
GKQGGRTLEIQRLIARSLRAMVDLEALGERSITLDCDVIQADGGTRTASITGACVALCDAIHGLVENGTLKVNPIKSLVA
AISVGIVEGVPVCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLALAKEGCQLIFDVQRQALAK

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=81.5165876777251, Blast_Score=364, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71981632, Length=187, Percent_Identity=27.807486631016, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_PASMU (Q9CJW5)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246815.1
- ProteinModelPortal:   Q9CJW5
- SMR:   Q9CJW5
- PRIDE:   Q9CJW5
- GeneID:   1245223
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1876
- NMPDR:   fig|272843.1.peg.1877
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   PMUL272843:PM1876-MONOMER
- BRENDA:   2.7.7.56
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25903; Mature: 25903

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPNERANNQPRPIKITRHYTKHAEGSVLVEFGDTKVLCTATVEESVPRFLKGQNQGWVT
CCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCEEEEEECHHHHHHHHHCCCCCCEEE
AEYGMLPRSTHSRMQREAAKGKQGGRTLEIQRLIARSLRAMVDLEALGERSITLDCDVIQ
EECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
ADGGTRTASITGACVALCDAIHGLVENGTLKVNPIKSLVAAISVGIVEGVPVCDLEYVED
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCHHHHHC
SAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLALAKEGCQLIFDVQRQALAK
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPNERANNQPRPIKITRHYTKHAEGSVLVEFGDTKVLCTATVEESVPRFLKGQNQGWVT
CCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCEEEEEECHHHHHHHHHCCCCCCEEE
AEYGMLPRSTHSRMQREAAKGKQGGRTLEIQRLIARSLRAMVDLEALGERSITLDCDVIQ
EECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
ADGGTRTASITGACVALCDAIHGLVENGTLKVNPIKSLVAAISVGIVEGVPVCDLEYVED
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCHHHHHC
SAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLALAKEGCQLIFDVQRQALAK
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100