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The map label for this gene is pyrE

Identifier: 15603742

GI number: 15603742

Start: 2119588

End: 2120232

Strand: Direct

Name: pyrE

Synonym: PM1877

Alternate gene names: 15603742

Gene position: 2119588-2120232 (Clockwise)

Preceding gene: 15603741

Following gene: 15603743

Centisome position: 93.89

GC content: 40.62

Gene sequence:

>645_bases
ATGGAAGCCTATAAATCGGAATTTATTCAATTTGCGCTAAGCCGTCAGGTGTTAAAATTTGGTGAGTTTACTCTAAAATC
TGGTCGTATTAGTCCCTATTTTTTTAATGCAGGGTTATTTAATACGGGAGCAGATCTCGCTCGTTTAGGGGAATTCTATG
CTAAAGCGATTCAAGCCAGTGGTGTTGAATATGATGTGATTTTTGGTCCGGCTTATAAAGGGCTGCCGATTGCCACGACG
GTTTCTGTGGCATTATTTAATCATTTTCAAATTGATAAACCCGTTTGCTTTAACCGTAAAGAAGCCAAAGATCACGGTGA
AGGGGGGCAGCTCATTGGCTATGGTTTAAGTGGAAAAATTTTATTGGTCGATGATGTGATTACTGCAGGGACCGCCATTC
GTGAATCCATGACCTTAATTGCACAAAATCAAGCGGAACTCAGTGCCGTGATGATCGCGTTAAATCGTCAAGAAAAAGGC
AAAGGTGAATTGTCTGCGATTCAAGAAGTGGAACGTGATTATCAATGCCAAGTGTTGTCGATTGTGAATTTTGATGATTT
GATGACCTTCATTGAGCAAGCACCAGAATATCAACAATATTTACCAGCAATGCGTGCTTATCGTGAGCAATATGGCGTGA
AGTAA

Upstream 100 bases:

>100_bases
TGAAGAATTATTGACCTTGTTAGCCTTAGCCAAAGAAGGCTGTCAGCTTATTTTCGATGTGCAACGTCAGGCGCTAGCCA
AGTAGGAAGGAGAGCGGATC

Downstream 100 bases:

>100_bases
GATAAAAGTGCGGTGGGTTTTAAGAAAATATTCAGAATAGGACGATATTGTATAATGCACAACCGCACTTTGGCGCTTTT
GGAGGGGAAGAATGAATTTT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MEAYKSEFIQFALSRQVLKFGEFTLKSGRISPYFFNAGLFNTGADLARLGEFYAKAIQASGVEYDVIFGPAYKGLPIATT
VSVALFNHFQIDKPVCFNRKEAKDHGEGGQLIGYGLSGKILLVDDVITAGTAIRESMTLIAQNQAELSAVMIALNRQEKG
KGELSAIQEVERDYQCQVLSIVNFDDLMTFIEQAPEYQQYLPAMRAYREQYGVK

Sequences:

>Translated_214_residues
MEAYKSEFIQFALSRQVLKFGEFTLKSGRISPYFFNAGLFNTGADLARLGEFYAKAIQASGVEYDVIFGPAYKGLPIATT
VSVALFNHFQIDKPVCFNRKEAKDHGEGGQLIGYGLSGKILLVDDVITAGTAIRESMTLIAQNQAELSAVMIALNRQEKG
KGELSAIQEVERDYQCQVLSIVNFDDLMTFIEQAPEYQQYLPAMRAYREQYGVK
>Mature_214_residues
MEAYKSEFIQFALSRQVLKFGEFTLKSGRISPYFFNAGLFNTGADLARLGEFYAKAIQASGVEYDVIFGPAYKGLPIATT
VSVALFNHFQIDKPVCFNRKEAKDHGEGGQLIGYGLSGKILLVDDVITAGTAIRESMTLIAQNQAELSAVMIALNRQEKG
KGELSAIQEVERDYQCQVLSIVNFDDLMTFIEQAPEYQQYLPAMRAYREQYGVK

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=180, Percent_Identity=26.6666666666667, Blast_Score=78, Evalue=5e-15,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=65.7276995305164, Blast_Score=309, Evalue=9e-86,
Organism=Caenorhabditis elegans, GI17554964, Length=191, Percent_Identity=30.3664921465969, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508631, Length=163, Percent_Identity=29.4478527607362, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323530, Length=216, Percent_Identity=47.2222222222222, Blast_Score=177, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6323927, Length=224, Percent_Identity=43.3035714285714, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_PASMU (Q9CJW4)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246816.1
- ProteinModelPortal:   Q9CJW4
- SMR:   Q9CJW4
- GeneID:   1245224
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1877
- NMPDR:   fig|272843.1.peg.1878
- HOGENOM:   HBG404341
- OMA:   FAFNRKE
- ProtClustDB:   PRK00455
- BioCyc:   PMUL272843:PM1877-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 23853; Mature: 23853

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 26-26 BINDING 99-99 BINDING 100-100 BINDING 103-103 BINDING 105-105 BINDING 128-128 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAYKSEFIQFALSRQVLKFGEFTLKSGRISPYFFNAGLFNTGADLARLGEFYAKAIQAS
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GVEYDVIFGPAYKGLPIATTVSVALFNHFQIDKPVCFNRKEAKDHGEGGQLIGYGLSGKI
CCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCEEEECCCCCE
LLVDDVITAGTAIRESMTLIAQNQAELSAVMIALNRQEKGKGELSAIQEVERDYQCQVLS
EEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEE
IVNFDDLMTFIEQAPEYQQYLPAMRAYREQYGVK
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEAYKSEFIQFALSRQVLKFGEFTLKSGRISPYFFNAGLFNTGADLARLGEFYAKAIQAS
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GVEYDVIFGPAYKGLPIATTVSVALFNHFQIDKPVCFNRKEAKDHGEGGQLIGYGLSGKI
CCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCEEEECCCCCE
LLVDDVITAGTAIRESMTLIAQNQAELSAVMIALNRQEKGKGELSAIQEVERDYQCQVLS
EEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEE
IVNFDDLMTFIEQAPEYQQYLPAMRAYREQYGVK
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100