Click here to switch to the map view.

The map label for this gene is brnQ [C]

Identifier: 15603738

GI number: 15603738

Start: 2115595

End: 2116899

Strand: Direct

Name: brnQ [C]

Synonym: PM1873

Alternate gene names: 15603738

Gene position: 2115595-2116899 (Clockwise)

Preceding gene: 15603735

Following gene: 15603741

Centisome position: 93.71

GC content: 40.54

Gene sequence:

>1305_bases
ATGAACAAAAACACATTTGTTGTTGGCTTTATGCTATTTGCCATCTTTTTTGGGGCTGGGAATTTAATTTTTCCACCCAA
ATTAGGTTTTGAAAGTGGCGTCGGTTTTTGGCCAGCTATTCTTGGGTTTGTCACCACAGGCGTTGGTTTGCCTCTTTTAG
GGATTTTAGTGGCGACTCGTTATGAAGGTGGCTATAAGAAAGCATTAGAAGATATTCATCCTTGGATTTCTGTGCTCTTG
TTAGGTGCGATTTATTTAACGATTGGACCGTTTTTTGCGATCCCACGTACCGCGGCGACCGCTTTTGATATGGCAGTGAT
TCCTTTTATTGGGAGCAGCGATCCTGTTTCTTTATTTGCCTTTACCTTTGTTTATTTTGCGATCACATTATGGGTCAGTT
TAAACCCGACTAAAATGGTGGATCGTATCGGGGCGATTTTAACTCCTGTTCTATTAGTTGCAATTATTGCGTTAGTCGTA
CGTATGGCGAGTATTTTAATTAGCGGAGAGTACAATAATACCTCAGCAGGTATGGAAACCCCATTAATTGTTGGCTTTAT
CGAAGGCTATCAAACCATGGATGTGCTTGCCTCTTTTGCTTTCTCTGTCGTGGTCATGAACGCAATTCGTGCGAAAAGTG
ACAAAAAAACCAGCTTTATTAAACAAGCCACGGTAGCAAGTGTGATTGCGGCAATTGCTTTAGCCTTTATTTATATTGCG
ATTGGTTGGATTGGCAACAATATCCCGTTGAGTGCGGAAACTGTTGCAGATGTTGCGAGCAAAGGTCAAAATTTAGGCGT
ATTTATTTTAAATGATGCAACGACACAATCGTTTGGTGAGCTAGGACGTAGCTTACTGGGCTTAATTGTGACATTAGCTT
GTTTAACAACAGCGATTGGTTTAGTGGTGGCAACCTCTTCTTACTTTAACAGTATTTTCCCTGTCATTTCTTATCGTAAC
TATGCCATTTTATTTACCTTGATTGGATTTGGTTTGGCGAACCAAGGGTTAAATGCCGTGATTAGTAAGTCTGTGCCTGT
GTTATTAGTCCTTTATCCAATCTCAATGACAGCGATGTTATTATTGTTAGTTAATTTGGTCATGCCATTACCAAAATTAG
CAAAAGGCATCCCATTAGTGTTGGTGGCGATTGTGTCCATCTTATCCGTCATGGGCGCTGAGGTTGTAGCAGGCTTACCA
TTTAAAGCATACTCAATGGAATGGTTACCGGTGGCATTATTTGGTTGTTTCATCGGCTTAATCATTGTAAAAGCGCAAGC
GCAAATGAAAGTGAAAGCACATTAA

Upstream 100 bases:

>100_bases
CTCACTTTTGTAGGTATTGATGATTTTTTATAGTAGAATGTCGCGTTTTTCTATTTTTAATATCCTAAAAAATTCTGTTT
TTTAATATTTGAGGTTTTCT

Downstream 100 bases:

>100_bases
GTTTATTGTGAGTATTAAAAAAGCAGATCATATGATCTGCTTTTTTGTGCCTGAATGTTAACTAACTTAATGTATAAGGC
AAGGGAAACCGGGTTAAAGC

Product: BrnQ

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNKNTFVVGFMLFAIFFGAGNLIFPPKLGFESGVGFWPAILGFVTTGVGLPLLGILVATRYEGGYKKALEDIHPWISVLL
LGAIYLTIGPFFAIPRTAATAFDMAVIPFIGSSDPVSLFAFTFVYFAITLWVSLNPTKMVDRIGAILTPVLLVAIIALVV
RMASILISGEYNNTSAGMETPLIVGFIEGYQTMDVLASFAFSVVVMNAIRAKSDKKTSFIKQATVASVIAAIALAFIYIA
IGWIGNNIPLSAETVADVASKGQNLGVFILNDATTQSFGELGRSLLGLIVTLACLTTAIGLVVATSSYFNSIFPVISYRN
YAILFTLIGFGLANQGLNAVISKSVPVLLVLYPISMTAMLLLLVNLVMPLPKLAKGIPLVLVAIVSILSVMGAEVVAGLP
FKAYSMEWLPVALFGCFIGLIIVKAQAQMKVKAH

Sequences:

>Translated_434_residues
MNKNTFVVGFMLFAIFFGAGNLIFPPKLGFESGVGFWPAILGFVTTGVGLPLLGILVATRYEGGYKKALEDIHPWISVLL
LGAIYLTIGPFFAIPRTAATAFDMAVIPFIGSSDPVSLFAFTFVYFAITLWVSLNPTKMVDRIGAILTPVLLVAIIALVV
RMASILISGEYNNTSAGMETPLIVGFIEGYQTMDVLASFAFSVVVMNAIRAKSDKKTSFIKQATVASVIAAIALAFIYIA
IGWIGNNIPLSAETVADVASKGQNLGVFILNDATTQSFGELGRSLLGLIVTLACLTTAIGLVVATSSYFNSIFPVISYRN
YAILFTLIGFGLANQGLNAVISKSVPVLLVLYPISMTAMLLLLVNLVMPLPKLAKGIPLVLVAIVSILSVMGAEVVAGLP
FKAYSMEWLPVALFGCFIGLIIVKAQAQMKVKAH
>Mature_434_residues
MNKNTFVVGFMLFAIFFGAGNLIFPPKLGFESGVGFWPAILGFVTTGVGLPLLGILVATRYEGGYKKALEDIHPWISVLL
LGAIYLTIGPFFAIPRTAATAFDMAVIPFIGSSDPVSLFAFTFVYFAITLWVSLNPTKMVDRIGAILTPVLLVAIIALVV
RMASILISGEYNNTSAGMETPLIVGFIEGYQTMDVLASFAFSVVVMNAIRAKSDKKTSFIKQATVASVIAAIALAFIYIA
IGWIGNNIPLSAETVADVASKGQNLGVFILNDATTQSFGELGRSLLGLIVTLACLTTAIGLVVATSSYFNSIFPVISYRN
YAILFTLIGFGLANQGLNAVISKSVPVLLVLYPISMTAMLLLLVNLVMPLPKLAKGIPLVLVAIVSILSVMGAEVVAGLP
FKAYSMEWLPVALFGCFIGLIIVKAQAQMKVKAH

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine), which is coupled to a proton motive force (Potential) [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=356, Percent_Identity=36.5168539325843, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46200; Mature: 46200

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNTFVVGFMLFAIFFGAGNLIFPPKLGFESGVGFWPAILGFVTTGVGLPLLGILVATR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
YEGGYKKALEDIHPWISVLLLGAIYLTIGPFFAIPRTAATAFDMAVIPFIGSSDPVSLFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHH
FTFVYFAITLWVSLNPTKMVDRIGAILTPVLLVAIIALVVRMASILISGEYNNTSAGMET
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PLIVGFIEGYQTMDVLASFAFSVVVMNAIRAKSDKKTSFIKQATVASVIAAIALAFIYIA
CHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IGWIGNNIPLSAETVADVASKGQNLGVFILNDATTQSFGELGRSLLGLIVTLACLTTAIG
HHHCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVVATSSYFNSIFPVISYRNYAILFTLIGFGLANQGLNAVISKSVPVLLVLYPISMTAML
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHHH
LLLVNLVMPLPKLAKGIPLVLVAIVSILSVMGAEVVAGLPFKAYSMEWLPVALFGCFIGL
HHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHH
IIVKAQAQMKVKAH
HHHHHHHHEEECCC
>Mature Secondary Structure
MNKNTFVVGFMLFAIFFGAGNLIFPPKLGFESGVGFWPAILGFVTTGVGLPLLGILVATR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
YEGGYKKALEDIHPWISVLLLGAIYLTIGPFFAIPRTAATAFDMAVIPFIGSSDPVSLFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHH
FTFVYFAITLWVSLNPTKMVDRIGAILTPVLLVAIIALVVRMASILISGEYNNTSAGMET
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PLIVGFIEGYQTMDVLASFAFSVVVMNAIRAKSDKKTSFIKQATVASVIAAIALAFIYIA
CHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IGWIGNNIPLSAETVADVASKGQNLGVFILNDATTQSFGELGRSLLGLIVTLACLTTAIG
HHHCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVVATSSYFNSIFPVISYRNYAILFTLIGFGLANQGLNAVISKSVPVLLVLYPISMTAML
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHHH
LLLVNLVMPLPKLAKGIPLVLVAIVSILSVMGAEVVAGLPFKAYSMEWLPVALFGCFIGL
HHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHH
IIVKAQAQMKVKAH
HHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA