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The map label for this gene is mesJ

Identifier: 15602154

GI number: 15602154

Start: 329899

End: 331194

Strand: Reverse

Name: mesJ

Synonym: PM0289

Alternate gene names: 15602154

Gene position: 331194-329899 (Counterclockwise)

Preceding gene: 15602155

Following gene: 15602153

Centisome position: 14.67

GC content: 44.21

Gene sequence:

>1296_bases
ATGTTGCTAACACAATTCCAAGCCGAAATTGCGCAACTTCCGCCCACACAACATCAGTTTTTAATTGGTTTTAGTGGTGG
TTTGGATTCCACTGCTCTATTACTCTTGTTTGCAAAACTTCGTGAAAAACAACCGCACTTAATGCTACGGGCGATTCATA
TTCATCACGGACTAAGTGCTAACGCGGATGCATGGGTCACGCATTGCCAGCAAATTTGCCAACAAGTAGCCATTCCACTC
ATTGTAGAACAGGTCAAAATTGATCAGCACAAAGGGATTGAAGCGGGAGCAAGAGAAGCACGCTATCAAGCAATCCGCCA
ACATCTTCTGCCTAATGAAATGTTCGCCACAGCACATCATCAGCAAGATCAAACCGAAACCTTTTTCCTTGCCTTAAAAC
GAGGTAGTGGCTTGGCTGGTCTCGGTGCGATGCAAAGGAAAAGTACACTCTATTGTTTACCTATCTGTCGTCCATTATTA
ACCTTTACACGCCAAATGTTGTTAGATTATGTCAAGCAAGCAGGTTGCCCTTGGGTAGAAGATGAGAGCAATACAGACAG
CCGTTATGAGCGCAATTTCTTACGCCATGAAGTCCTTCCACGCTTACGGCAACGCTGGCCACATTTTGACAATGCTGTGC
AGCGCAGCGCACAACATTGCTTTGAGCAACAACAATTACTCAATGAACTGCTACACGCCGAATTTGCACAACATTTTGAT
AAAACCGAGCGCACTTTTCAGCTCCGCGATTTTGCTACACGCTCTCAGTTAAAACAAAATGCATTACTCCGTTTATGGTT
GGCGCAATGTGGGCTTTCTATGCCGTCTAAAGTGCAGTTAACGCAATTGATTCAAGATGTCATTCTTGCTAAATCGGATA
GCCAACCCCAATTTCAACTGGGCGAATATTGTGTCCGTCGCTATCAGGATACACTCTACCTGACACCCCATTTTGCTGAT
TTATCATCACTGCGCTTACCAATTCACATTGGCGAAACACTTACCTTACCCGATCATTTAGGCACACTTTCACTCCAACA
ACAAGCTGGGCAATTGCAGGTGATTTGGTCTCATCCACAACACACCTTCACAAGCATATTGCCTTTAACAGAAGATCCTA
TCGAAGTACGTTTTCGCTATTCCGGTAAAGTGCGTTTAAGCCCAACTAGTGCGAATAAAGACATCAAAAAACTCTGGCAG
CAACATGCTGTCCCAGTATGGTTACGCCAACGTATTCCACTTATTTTCTATGCGGACAGGTTCAAAAGTGCGGTCGGTTA
TTTCAATGCTATCTAA

Upstream 100 bases:

>100_bases
ATTATGCAACCTAGCAGTCAATATAAAGCCGTGAAAATTGCTTAAATTCTTGCAAAAAGGGCGTTAGAATTAACGTCCTT
TTACTTTGTGATGAAATACT

Downstream 100 bases:

>100_bases
TGCCAGCTTTCCGCTTTACTTTTCTTTTTGCTGTTATCTCTTCTGACATTGTCATGAGCAGCGCAGCATAACCTCGCTTT
CGTTATCGATACTGTTAGCC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MLLTQFQAEIAQLPPTQHQFLIGFSGGLDSTALLLLFAKLREKQPHLMLRAIHIHHGLSANADAWVTHCQQICQQVAIPL
IVEQVKIDQHKGIEAGAREARYQAIRQHLLPNEMFATAHHQQDQTETFFLALKRGSGLAGLGAMQRKSTLYCLPICRPLL
TFTRQMLLDYVKQAGCPWVEDESNTDSRYERNFLRHEVLPRLRQRWPHFDNAVQRSAQHCFEQQQLLNELLHAEFAQHFD
KTERTFQLRDFATRSQLKQNALLRLWLAQCGLSMPSKVQLTQLIQDVILAKSDSQPQFQLGEYCVRRYQDTLYLTPHFAD
LSSLRLPIHIGETLTLPDHLGTLSLQQQAGQLQVIWSHPQHTFTSILPLTEDPIEVRFRYSGKVRLSPTSANKDIKKLWQ
QHAVPVWLRQRIPLIFYADRFKSAVGYFNAI

Sequences:

>Translated_431_residues
MLLTQFQAEIAQLPPTQHQFLIGFSGGLDSTALLLLFAKLREKQPHLMLRAIHIHHGLSANADAWVTHCQQICQQVAIPL
IVEQVKIDQHKGIEAGAREARYQAIRQHLLPNEMFATAHHQQDQTETFFLALKRGSGLAGLGAMQRKSTLYCLPICRPLL
TFTRQMLLDYVKQAGCPWVEDESNTDSRYERNFLRHEVLPRLRQRWPHFDNAVQRSAQHCFEQQQLLNELLHAEFAQHFD
KTERTFQLRDFATRSQLKQNALLRLWLAQCGLSMPSKVQLTQLIQDVILAKSDSQPQFQLGEYCVRRYQDTLYLTPHFAD
LSSLRLPIHIGETLTLPDHLGTLSLQQQAGQLQVIWSHPQHTFTSILPLTEDPIEVRFRYSGKVRLSPTSANKDIKKLWQ
QHAVPVWLRQRIPLIFYADRFKSAVGYFNAI
>Mature_431_residues
MLLTQFQAEIAQLPPTQHQFLIGFSGGLDSTALLLLFAKLREKQPHLMLRAIHIHHGLSANADAWVTHCQQICQQVAIPL
IVEQVKIDQHKGIEAGAREARYQAIRQHLLPNEMFATAHHQQDQTETFFLALKRGSGLAGLGAMQRKSTLYCLPICRPLL
TFTRQMLLDYVKQAGCPWVEDESNTDSRYERNFLRHEVLPRLRQRWPHFDNAVQRSAQHCFEQQQLLNELLHAEFAQHFD
KTERTFQLRDFATRSQLKQNALLRLWLAQCGLSMPSKVQLTQLIQDVILAKSDSQPQFQLGEYCVRRYQDTLYLTPHFAD
LSSLRLPIHIGETLTLPDHLGTLSLQQQAGQLQVIWSHPQHTFTSILPLTEDPIEVRFRYSGKVRLSPTSANKDIKKLWQ
QHAVPVWLRQRIPLIFYADRFKSAVGYFNAI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=415, Percent_Identity=42.6506024096386, Blast_Score=319, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_PASMU (Q9CNY2)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245226.1
- ProteinModelPortal:   Q9CNY2
- SMR:   Q9CNY2
- GeneID:   1243636
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0289
- NMPDR:   fig|272843.1.peg.289
- HOGENOM:   HBG668702
- OMA:   KSAVGFF
- ProtClustDB:   CLSK870038
- BioCyc:   PMUL272843:PM0289-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 49741; Mature: 49741

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTQFQAEIAQLPPTQHQFLIGFSGGLDSTALLLLFAKLREKQPHLMLRAIHIHHGLSA
CCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
NADAWVTHCQQICQQVAIPLIVEQVKIDQHKGIEAGAREARYQAIRQHLLPNEMFATAHH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHC
QQDQTETFFLALKRGSGLAGLGAMQRKSTLYCLPICRPLLTFTRQMLLDYVKQAGCPWVE
CCCCCCEEEEEEECCCCCCCCCHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DESNTDSRYERNFLRHEVLPRLRQRWPHFDNAVQRSAQHCFEQQQLLNELLHAEFAQHFD
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTERTFQLRDFATRSQLKQNALLRLWLAQCGLSMPSKVQLTQLIQDVILAKSDSQPQFQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
GEYCVRRYQDTLYLTPHFADLSSLRLPIHIGETLTLPDHLGTLSLQQQAGQLQVIWSHPQ
HHHHHHHHCCCEEECCCCCCHHHCEEEEEECCEEECCHHHCCHHHHHCCCCEEEEECCCC
HTFTSILPLTEDPIEVRFRYSGKVRLSPTSANKDIKKLWQQHAVPVWLRQRIPLIFYADR
HHHHHHCCCCCCCEEEEEEECCEEEECCCCCCHHHHHHHHHCCCCHHHHCCCCEEEEHHH
FKSAVGYFNAI
HHHHHHHHCCC
>Mature Secondary Structure
MLLTQFQAEIAQLPPTQHQFLIGFSGGLDSTALLLLFAKLREKQPHLMLRAIHIHHGLSA
CCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
NADAWVTHCQQICQQVAIPLIVEQVKIDQHKGIEAGAREARYQAIRQHLLPNEMFATAHH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHC
QQDQTETFFLALKRGSGLAGLGAMQRKSTLYCLPICRPLLTFTRQMLLDYVKQAGCPWVE
CCCCCCEEEEEEECCCCCCCCCHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DESNTDSRYERNFLRHEVLPRLRQRWPHFDNAVQRSAQHCFEQQQLLNELLHAEFAQHFD
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTERTFQLRDFATRSQLKQNALLRLWLAQCGLSMPSKVQLTQLIQDVILAKSDSQPQFQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
GEYCVRRYQDTLYLTPHFADLSSLRLPIHIGETLTLPDHLGTLSLQQQAGQLQVIWSHPQ
HHHHHHHHCCCEEECCCCCCHHHCEEEEEECCEEECCHHHCCHHHHHCCCCEEEEECCCC
HTFTSILPLTEDPIEVRFRYSGKVRLSPTSANKDIKKLWQQHAVPVWLRQRIPLIFYADR
HHHHHHCCCCCCCEEEEEEECCEEEECCCCCCHHHHHHHHHCCCCHHHHCCCCEEEEHHH
FKSAVGYFNAI
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100