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The map label for this gene is potD_2 [H]

Identifier: 15602126

GI number: 15602126

Start: 293957

End: 295033

Strand: Reverse

Name: potD_2 [H]

Synonym: PM0261

Alternate gene names: 15602126

Gene position: 295033-293957 (Counterclockwise)

Preceding gene: 15602127

Following gene: 15602125

Centisome position: 13.07

GC content: 39.0

Gene sequence:

>1077_bases
ATGATTACCCTCAACAGGAGAATGATAGAGATGAAAAAATTTGCAGGTCTTGTGACTGCAGGGATCATGACATTCGGCTT
AGCCGCAAATGCACATGCAACCAATGACACTGTTTACCTTTATACTTGGACAGAATACGTTCCAGATGGTTTATTAGATG
ACTTCACAAAAGAAACGGGCATCAAAGTTATCGTTTCAAGCTTAGAATCAAACGAAACCATGTACGCCAAATTAAAGACA
CAAGGCGAAACCAGTGGTTATGATGTGATTGCACCATCAAACTATTTTGTGTCTAAAATGGCGCGTGAAGGTATGTTAAA
AGAGCTAGACCACAGCAAATTACCTGTTATCAAAGACTTAAATCCAGATTGGTTAGACAAGCCTTATGATAAAGGCAACA
AATACTCCTTGCCTCAGCTATTAGGTGCACCGGGTATTGCATTTAATACCGATAGCTATAAAGGTGAAAACTTTACTGCT
TGGGGTGATTTATGGAAACCTGAATTTAAAGGGAAAGTCCAATTATTAGATGACGCACGCGAAGTGTTTAATATTGCACT
GTTAAAATTGGGTCAAGATCCAAATACTACGGATCCCGCAATCATTAAACAAGCTTATGAAGAGCTGTTAAAATTACGTC
CAAACGTATTGTCATTTAACTCAGATAACCCAGCGAACTCATTCATTTCAGGTGAGGTGGAAGTGGGTCAATTATGGAAT
GGCTCAGTCCGTATTGCGAAAAAAGAACAAGCGCCGTTAAACATGGTTTTCCCGAAAGAAGGTCCTGTACTTTGGGTGGA
TACCTTAGCTATTCCAGCTAACTCGAAAAACCCTGATGGTGCACACAAATTAATTAACTATTTACTAAGTGCAAAAGTTG
CTGCACAGTTAGCAGAAGAAATTGGTTACCCAACATCAAACTTAAAAGCACTTGAATTATTGCCAAGCGAAGTGACGCAA
GATCCAGCAGTTTATCCAACGGCTGAAATTCTACAAAATAGCCACTGGCAAGATGATGTAGGTGATGCGGTTGAATTATA
CGAAAACTATTATCAACAATTAAAAGCGGCTAAATAA

Upstream 100 bases:

>100_bases
AATGTTGTCTTAAAAAATAATACTTCACAAACAACCTAATACAAAATAGAATACGCCTTGCCTTAAAACAAGGCGTTTTT
TTATGCTTCTTCAAGAAGAA

Downstream 100 bases:

>100_bases
TCGCGTTTTTATCATCCATAAACAGCAAGTGCGGTCAAAAAAATACAAATTTTGACCGCACTTTTTTTACTCTTTTATCT
TATGCAATTCACTTCTCATG

Product: PotD

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MITLNRRMIEMKKFAGLVTAGIMTFGLAANAHATNDTVYLYTWTEYVPDGLLDDFTKETGIKVIVSSLESNETMYAKLKT
QGETSGYDVIAPSNYFVSKMAREGMLKELDHSKLPVIKDLNPDWLDKPYDKGNKYSLPQLLGAPGIAFNTDSYKGENFTA
WGDLWKPEFKGKVQLLDDAREVFNIALLKLGQDPNTTDPAIIKQAYEELLKLRPNVLSFNSDNPANSFISGEVEVGQLWN
GSVRIAKKEQAPLNMVFPKEGPVLWVDTLAIPANSKNPDGAHKLINYLLSAKVAAQLAEEIGYPTSNLKALELLPSEVTQ
DPAVYPTAEILQNSHWQDDVGDAVELYENYYQQLKAAK

Sequences:

>Translated_358_residues
MITLNRRMIEMKKFAGLVTAGIMTFGLAANAHATNDTVYLYTWTEYVPDGLLDDFTKETGIKVIVSSLESNETMYAKLKT
QGETSGYDVIAPSNYFVSKMAREGMLKELDHSKLPVIKDLNPDWLDKPYDKGNKYSLPQLLGAPGIAFNTDSYKGENFTA
WGDLWKPEFKGKVQLLDDAREVFNIALLKLGQDPNTTDPAIIKQAYEELLKLRPNVLSFNSDNPANSFISGEVEVGQLWN
GSVRIAKKEQAPLNMVFPKEGPVLWVDTLAIPANSKNPDGAHKLINYLLSAKVAAQLAEEIGYPTSNLKALELLPSEVTQ
DPAVYPTAEILQNSHWQDDVGDAVELYENYYQQLKAAK
>Mature_358_residues
MITLNRRMIEMKKFAGLVTAGIMTFGLAANAHATNDTVYLYTWTEYVPDGLLDDFTKETGIKVIVSSLESNETMYAKLKT
QGETSGYDVIAPSNYFVSKMAREGMLKELDHSKLPVIKDLNPDWLDKPYDKGNKYSLPQLLGAPGIAFNTDSYKGENFTA
WGDLWKPEFKGKVQLLDDAREVFNIALLKLGQDPNTTDPAIIKQAYEELLKLRPNVLSFNSDNPANSFISGEVEVGQLWN
GSVRIAKKEQAPLNMVFPKEGPVLWVDTLAIPANSKNPDGAHKLINYLLSAKVAAQLAEEIGYPTSNLKALELLPSEVTQ
DPAVYPTAEILQNSHWQDDVGDAVELYENYYQQLKAAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=349, Percent_Identity=54.4412607449857, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1787078, Length=350, Percent_Identity=34.5714285714286, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1787711, Length=295, Percent_Identity=25.4237288135593, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39830; Mature: 39830

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLNRRMIEMKKFAGLVTAGIMTFGLAANAHATNDTVYLYTWTEYVPDGLLDDFTKETG
CEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHCC
IKVIVSSLESNETMYAKLKTQGETSGYDVIAPSNYFVSKMAREGMLKELDHSKLPVIKDL
HHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEECCC
NPDWLDKPYDKGNKYSLPQLLGAPGIAFNTDSYKGENFTAWGDLWKPEFKGKVQLLDDAR
CCHHCCCCCCCCCCCCCHHHHCCCCCEECCCCCCCCCEEECCCCCCCCCCCCEEEHHHHH
EVFNIALLKLGQDPNTTDPAIIKQAYEELLKLRPNVLSFNSDNPANSFISGEVEVGQLWN
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEECCCEEECEECC
GSVRIAKKEQAPLNMVFPKEGPVLWVDTLAIPANSKNPDGAHKLINYLLSAKVAAQLAEE
CEEEEEECCCCCCEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGYPTSNLKALELLPSEVTQDPAVYPTAEILQNSHWQDDVGDAVELYENYYQQLKAAK
HCCCCCCCHHHHHCHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITLNRRMIEMKKFAGLVTAGIMTFGLAANAHATNDTVYLYTWTEYVPDGLLDDFTKETG
CEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHCC
IKVIVSSLESNETMYAKLKTQGETSGYDVIAPSNYFVSKMAREGMLKELDHSKLPVIKDL
HHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEECCC
NPDWLDKPYDKGNKYSLPQLLGAPGIAFNTDSYKGENFTAWGDLWKPEFKGKVQLLDDAR
CCHHCCCCCCCCCCCCCHHHHCCCCCEECCCCCCCCCEEECCCCCCCCCCCCEEEHHHHH
EVFNIALLKLGQDPNTTDPAIIKQAYEELLKLRPNVLSFNSDNPANSFISGEVEVGQLWN
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEECCCEEECEECC
GSVRIAKKEQAPLNMVFPKEGPVLWVDTLAIPANSKNPDGAHKLINYLLSAKVAAQLAEE
CEEEEEECCCCCCEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGYPTSNLKALELLPSEVTQDPAVYPTAEILQNSHWQDDVGDAVELYENYYQQLKAAK
HCCCCCCCHHHHHCHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]