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The map label for this gene is potD_1 [H]

Identifier: 15602125

GI number: 15602125

Start: 292651

End: 293697

Strand: Reverse

Name: potD_1 [H]

Synonym: PM0260

Alternate gene names: 15602125

Gene position: 293697-292651 (Counterclockwise)

Preceding gene: 15602126

Following gene: 15602124

Centisome position: 13.01

GC content: 37.63

Gene sequence:

>1047_bases
ATGAAAAAATTAACTGGCATTTTAACTGCTAGCGTGATGATGCTGGGCGTAAGCAACTATGCAAGCGCAGCTAACAATGA
AGTTTACCTTTATACTTGGACTGAATATGTTCCTGAAGGTTTATTAGATGAATTTACCAAACAAACGGGAATTAAGGTCA
TTGTTTCAAGCCTTGAGTCAAACGAAACCATGTATGCCAAATTAAAAACTTTGGGGAAAGACGGTGGCTATGATGTGATT
GCACCAACTAGCTATTTTGTGTCCAAAATGGCACGTGAAGGCATGCTAAAAGAGCTTGATCACAGTAAATTACCGGTCAT
TAAAGAGCTTGATCCAAATATGCTTGATCGCCCTTACGATAAAGGCAATAAATATTCATTACCACAACTCTTTGGTGCGA
CTGGCATTGCTTATAATACCACCAACCAAGATCCAAGCCATTTCCAATCTTGGGGGGATTTATGGAATGCCGAATTCAAA
GGGAAAGTACAAATGCTCGATGACCCTCGTGAACTGTTTAATATTGCTTTATTAAAAATGGGGAAAGATCCTAATACTCA
AGATCCAGCAGTATTAAAAGAAGCCTATGAGGAATTATTAAAATTACGTCCAAATATTCTTTCTTTTAACTCGGATAACC
CTGCCAGTACTTTTATTTCAGGTGAAACCGATATTGGCTTGCTGTGGAATGGTTCCATTCGTATCGCCAAAAATGAAGGG
GCAACGGTAGAGATGATTTATCCAAAAGAAGGCACTATGTTATGGATAGATAATCTTGCTATCCCTTCCAATGCGAAAAA
TACGGAAAATGCTTATAAGCTCATTAACTATTTATTGAGTACAGATGTATCCGAAAAACTGACTTTAAATATCGGCTATC
CAACCTCAAACCTTGAAGCGTTAAAACGCTTACCAAAAGAATTAACCGATGATCATGCAACGTTCTTAACACAAGAGGTT
ATTGATAGAAGCCAATGGCAAGTGGACGTAGATAATGCTATCGATTTGTATGAAGGGTATTATCAAAAGCTCAAAGCAGC
AAAATAA

Upstream 100 bases:

>100_bases
CCTAAAATCCGTCTTTGTCCGTACCCATTCGGCATCTTCAGGTCAGTAAACGCCCTCGTTTTACTGGCATATTTTCACCC
ATTTTCTGGGAGCAATGTGA

Downstream 100 bases:

>100_bases
TTCACTATCGATTTGATTTGTATCATAAAAAGATCTTCTGAATGAAGATCTTTTTTTTCTGTTGCATTAAGATGAGGTCA
TGCATGATGAGAAGGAGAAA

Product: PotD

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKKLTGILTASVMMLGVSNYASAANNEVYLYTWTEYVPEGLLDEFTKQTGIKVIVSSLESNETMYAKLKTLGKDGGYDVI
APTSYFVSKMAREGMLKELDHSKLPVIKELDPNMLDRPYDKGNKYSLPQLFGATGIAYNTTNQDPSHFQSWGDLWNAEFK
GKVQMLDDPRELFNIALLKMGKDPNTQDPAVLKEAYEELLKLRPNILSFNSDNPASTFISGETDIGLLWNGSIRIAKNEG
ATVEMIYPKEGTMLWIDNLAIPSNAKNTENAYKLINYLLSTDVSEKLTLNIGYPTSNLEALKRLPKELTDDHATFLTQEV
IDRSQWQVDVDNAIDLYEGYYQKLKAAK

Sequences:

>Translated_348_residues
MKKLTGILTASVMMLGVSNYASAANNEVYLYTWTEYVPEGLLDEFTKQTGIKVIVSSLESNETMYAKLKTLGKDGGYDVI
APTSYFVSKMAREGMLKELDHSKLPVIKELDPNMLDRPYDKGNKYSLPQLFGATGIAYNTTNQDPSHFQSWGDLWNAEFK
GKVQMLDDPRELFNIALLKMGKDPNTQDPAVLKEAYEELLKLRPNILSFNSDNPASTFISGETDIGLLWNGSIRIAKNEG
ATVEMIYPKEGTMLWIDNLAIPSNAKNTENAYKLINYLLSTDVSEKLTLNIGYPTSNLEALKRLPKELTDDHATFLTQEV
IDRSQWQVDVDNAIDLYEGYYQKLKAAK
>Mature_348_residues
MKKLTGILTASVMMLGVSNYASAANNEVYLYTWTEYVPEGLLDEFTKQTGIKVIVSSLESNETMYAKLKTLGKDGGYDVI
APTSYFVSKMAREGMLKELDHSKLPVIKELDPNMLDRPYDKGNKYSLPQLFGATGIAYNTTNQDPSHFQSWGDLWNAEFK
GKVQMLDDPRELFNIALLKMGKDPNTQDPAVLKEAYEELLKLRPNILSFNSDNPASTFISGETDIGLLWNGSIRIAKNEG
ATVEMIYPKEGTMLWIDNLAIPSNAKNTENAYKLINYLLSTDVSEKLTLNIGYPTSNLEALKRLPKELTDDHATFLTQEV
IDRSQWQVDVDNAIDLYEGYYQKLKAAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=324, Percent_Identity=51.5432098765432, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1787078, Length=338, Percent_Identity=31.3609467455621, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1787711, Length=305, Percent_Identity=25.5737704918033, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39117; Mature: 39117

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTGILTASVMMLGVSNYASAANNEVYLYTWTEYVPEGLLDEFTKQTGIKVIVSSLES
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHCCHHHHHHHHHHCCHHHHHHHHCC
NETMYAKLKTLGKDGGYDVIAPTSYFVSKMAREGMLKELDHSKLPVIKELDPNMLDRPYD
CCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHCCCCC
KGNKYSLPQLFGATGIAYNTTNQDPSHFQSWGDLWNAEFKGKVQMLDDPRELFNIALLKM
CCCCCCCHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHC
GKDPNTQDPAVLKEAYEELLKLRPNILSFNSDNPASTFISGETDIGLLWNGSIRIAKNEG
CCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCEEEEECCEEEEEECCC
ATVEMIYPKEGTMLWIDNLAIPSNAKNTENAYKLINYLLSTDVSEKLTLNIGYPTSNLEA
CEEEEEECCCCCEEEEECEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHH
LKRLPKELTDDHATFLTQEVIDRSQWQVDVDNAIDLYEGYYQKLKAAK
HHHHHHHHCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLTGILTASVMMLGVSNYASAANNEVYLYTWTEYVPEGLLDEFTKQTGIKVIVSSLES
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHCCHHHHHHHHHHCCHHHHHHHHCC
NETMYAKLKTLGKDGGYDVIAPTSYFVSKMAREGMLKELDHSKLPVIKELDPNMLDRPYD
CCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHCCCCC
KGNKYSLPQLFGATGIAYNTTNQDPSHFQSWGDLWNAEFKGKVQMLDDPRELFNIALLKM
CCCCCCCHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHC
GKDPNTQDPAVLKEAYEELLKLRPNILSFNSDNPASTFISGETDIGLLWNGSIRIAKNEG
CCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCEEEEECCEEEEEECCC
ATVEMIYPKEGTMLWIDNLAIPSNAKNTENAYKLINYLLSTDVSEKLTLNIGYPTSNLEA
CEEEEEECCCCCEEEEECEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHH
LKRLPKELTDDHATFLTQEVIDRSQWQVDVDNAIDLYEGYYQKLKAAK
HHHHHHHHCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]