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The map label for this gene is ampD [H]

Identifier: 15601948

GI number: 15601948

Start: 110243

End: 110809

Strand: Reverse

Name: ampD [H]

Synonym: PM0083

Alternate gene names: 15601948

Gene position: 110809-110243 (Counterclockwise)

Preceding gene: 15601958

Following gene: 15601947

Centisome position: 4.91

GC content: 39.15

Gene sequence:

>567_bases
ATGACAAAAAAAGCAAAGCAACATATTCAGCAAGGGTGGCTAAAGGGGGCCAAAAGAGTCCTTTCTCCACATTATGATCA
ACGTCCAGATCTACATGACATTTCTCTACTTATTATCCACTATATTAGCCTCCCACCAGAGCAATTTGGCGGACACTATA
TTGATGATTTTTTCCAAGGCAAACTAAATCCTACACAACATCCTTATTTTGAAGAAATTGCAACATTACGTGTTTCTGCA
CATTGTTTAATCAACCGACAAGGTGAAATTACACAATATGTCAATTTTAATGATAGGGCTTGGCATGCTGGTGTCTCGCA
ATTCGAAGGAAGAGAAAAATGCAATGATTTTTCTATTGGTATTGAATTAGAAGGAAGTAATACTCAGCCTTTCACCGTCG
CGCAATATCAATCATTAATCACTCTCACAAAAGCGATTATGGCAAGTTATCCAGACATTACATTACAACGGATTGTGGGG
CATTGCGATGTTGCGCCAGGTCGCAAAATCGATCCGGGTCAATATTTTGACTGGCAGACTTATCTAGATGCACTACAAAA
CCAGTGA

Upstream 100 bases:

>100_bases
TGGACTGGCATTTTCTCGCCAACGAAGTATGTAATAAAAAGGTTTACTCAGAAAAAAACACAAATTACTTTTTCGATCTT
TATCGCAAAACGGATCACTT

Downstream 100 bases:

>100_bases
ACAAAAAACAAACACAAGAAATGACTAATTCATCACAACTAACAGAAAAGCAACATAAAACCTTATCTATTTGATTTATA
AGACAATAAACACATATCAC

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MTKKAKQHIQQGWLKGAKRVLSPHYDQRPDLHDISLLIIHYISLPPEQFGGHYIDDFFQGKLNPTQHPYFEEIATLRVSA
HCLINRQGEITQYVNFNDRAWHAGVSQFEGREKCNDFSIGIELEGSNTQPFTVAQYQSLITLTKAIMASYPDITLQRIVG
HCDVAPGRKIDPGQYFDWQTYLDALQNQ

Sequences:

>Translated_188_residues
MTKKAKQHIQQGWLKGAKRVLSPHYDQRPDLHDISLLIIHYISLPPEQFGGHYIDDFFQGKLNPTQHPYFEEIATLRVSA
HCLINRQGEITQYVNFNDRAWHAGVSQFEGREKCNDFSIGIELEGSNTQPFTVAQYQSLITLTKAIMASYPDITLQRIVG
HCDVAPGRKIDPGQYFDWQTYLDALQNQ
>Mature_187_residues
TKKAKQHIQQGWLKGAKRVLSPHYDQRPDLHDISLLIIHYISLPPEQFGGHYIDDFFQGKLNPTQHPYFEEIATLRVSAH
CLINRQGEITQYVNFNDRAWHAGVSQFEGREKCNDFSIGIELEGSNTQPFTVAQYQSLITLTKAIMASYPDITLQRIVGH
CDVAPGRKIDPGQYFDWQTYLDALQNQ

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=173, Percent_Identity=61.271676300578, Blast_Score=234, Evalue=3e-63,
Organism=Escherichia coli, GI1787092, Length=165, Percent_Identity=37.5757575757576, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21590; Mature: 21458

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKAKQHIQQGWLKGAKRVLSPHYDQRPDLHDISLLIIHYISLPPEQFGGHYIDDFFQG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHCC
KLNPTQHPYFEEIATLRVSAHCLINRQGEITQYVNFNDRAWHAGVSQFEGREKCNDFSIG
CCCCCCCHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCHHHHCHHHHCCHHCCCCEEEE
IELEGSNTQPFTVAQYQSLITLTKAIMASYPDITLQRIVGHCDVAPGRKIDPGQYFDWQT
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHH
YLDALQNQ
HHHHHCCC
>Mature Secondary Structure 
TKKAKQHIQQGWLKGAKRVLSPHYDQRPDLHDISLLIIHYISLPPEQFGGHYIDDFFQG
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHCC
KLNPTQHPYFEEIATLRVSAHCLINRQGEITQYVNFNDRAWHAGVSQFEGREKCNDFSIG
CCCCCCCHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCHHHHCHHHHCCHHCCCCEEEE
IELEGSNTQPFTVAQYQSLITLTKAIMASYPDITLQRIVGHCDVAPGRKIDPGQYFDWQT
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHH
YLDALQNQ
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]