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The map label for this gene is tilS [H]

Identifier: 15595133

GI number: 15595133

Start: 824382

End: 825704

Strand: Direct

Name: tilS [H]

Synonym: BB0788

Alternate gene names: 15595133

Gene position: 824382-825704 (Clockwise)

Preceding gene: 15595132

Following gene: 15595134

Centisome position: 90.52

GC content: 25.93

Gene sequence:

>1323_bases
ATGCATTTTTTAGACGAAAATATACAAATTAAAATAGATAAATTTTATAAAAAAAATTCTTTAGATAAAAATCGGGTTAT
TGTTGCTTTTTCTGGCGGGGCTGATTCTACGGCTTTATTATTGAATTTAAAATATTACCTGAGCAATAATGTTATTGCAT
TTTATTTTGCTCATTTTATTAGATCTGATAATGAGCAAAATCAAGAGATAGAGCATGTAAAGGGGTTTTGCGATCTTTAC
AATATTGCTTTACAAATTAAAAAATGTGATATAGATATAAAAAGTGAATCAGCTAGGTTGGGAGTATCTATTGAGGAGCT
TGCAAGGAAATTTAGATATATTGCTTTAGAAAATGCTCTTAAAGAAAATGGCGCAAATTATATTGCACTTGCTCATAACG
AAAACGATCAAATTGAAACAATAATTATGAGATTTTTTCAAGGATCTTTTTTGGATGGTCTTTCAGGTATTCCTAGTGTC
AATAGAAACATTATAAGGCCCTTGCTTGAGGTTTCAAGACTAGAAATTGAAAATTTTTTATCTTTGAATAATATTGGGTT
TTTTGTTGATAGTACAAATGCTCAAAATTTATATCTAAGAAATAGGGTTAGAAATAATTTGCTACCCGCTATAAAAAAGG
TTTTTAAAGGATATGAAAAATGTCTTAAAAGAATATCTGAATTTTCAAAGGAATTTGCGGATTATTTTGGAAAAGATGAA
TTTTTTCCTGTTGAGAAAGGTAAATATTATTATTCTTTTGATCTGAAAACTTTCTTAGATTTTCCTAAGTATTTGGTATT
TAGATTGATTTTTAAAATTTTAAATTCAGAAGGAATTGCAGCCAAAGTTTCTTATAAAGCTCTTAATGAAGCGTTTAAAG
TAGAGATTAATAGGAAAAAAAATAATGTTTTGTTAAAAACCAATGATTTTTTTTTAGAAAAAAGGCATAATAAAATTAAT
TTAATTTTTAAAAGGGATGAAAAATTTTACAAACCTTTTGATTTTATTTTGGAAGTTGGTAAATGGCATAGTTTATCTTT
AGGTAAGATTTTGCTAAAATATTTAGAGTGTAATGCAGCTTCTGTATCAAGATTAAAATGTTGTTCTTATGAGTTTAGGT
ATAAATTTTTTAAGGATAGATTGAAAGCAAAAAAATTTTTTTCTAAGTTTATAAGGTGTAATCCGGCTTATTTAATGTTG
TTAGCATTAGATAACAGGTTAATTGGGATTATTGATTTAAATACTTTAAACTTAGTATGGAGTGAAAAAAGCATTCTTAA
AAAAATTAATATATCTTTGATTGGAGGGCTTTTAAAGGAATGA

Upstream 100 bases:

>100_bases
GACTTGAAAAATTGTATGATTTTTTAAGTGATGAGCTGATTGATATTAGCGAAGCAAATTTTAAAAATAAGGTTCAAAAA
ATTAATTCTAGTAATTTTTA

Downstream 100 bases:

>100_bases
ATGGCAATAATAATATGAATAACAATGGAAAATCTAACAACAAAAAAAAGAATAAAAATTGGATTTTAGGACTTGTTGTT
GTTTTTCTAATTTCAGCAAT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MHFLDENIQIKIDKFYKKNSLDKNRVIVAFSGGADSTALLLNLKYYLSNNVIAFYFAHFIRSDNEQNQEIEHVKGFCDLY
NIALQIKKCDIDIKSESARLGVSIEELARKFRYIALENALKENGANYIALAHNENDQIETIIMRFFQGSFLDGLSGIPSV
NRNIIRPLLEVSRLEIENFLSLNNIGFFVDSTNAQNLYLRNRVRNNLLPAIKKVFKGYEKCLKRISEFSKEFADYFGKDE
FFPVEKGKYYYSFDLKTFLDFPKYLVFRLIFKILNSEGIAAKVSYKALNEAFKVEINRKKNNVLLKTNDFFLEKRHNKIN
LIFKRDEKFYKPFDFILEVGKWHSLSLGKILLKYLECNAASVSRLKCCSYEFRYKFFKDRLKAKKFFSKFIRCNPAYLML
LALDNRLIGIIDLNTLNLVWSEKSILKKINISLIGGLLKE

Sequences:

>Translated_440_residues
MHFLDENIQIKIDKFYKKNSLDKNRVIVAFSGGADSTALLLNLKYYLSNNVIAFYFAHFIRSDNEQNQEIEHVKGFCDLY
NIALQIKKCDIDIKSESARLGVSIEELARKFRYIALENALKENGANYIALAHNENDQIETIIMRFFQGSFLDGLSGIPSV
NRNIIRPLLEVSRLEIENFLSLNNIGFFVDSTNAQNLYLRNRVRNNLLPAIKKVFKGYEKCLKRISEFSKEFADYFGKDE
FFPVEKGKYYYSFDLKTFLDFPKYLVFRLIFKILNSEGIAAKVSYKALNEAFKVEINRKKNNVLLKTNDFFLEKRHNKIN
LIFKRDEKFYKPFDFILEVGKWHSLSLGKILLKYLECNAASVSRLKCCSYEFRYKFFKDRLKAKKFFSKFIRCNPAYLML
LALDNRLIGIIDLNTLNLVWSEKSILKKINISLIGGLLKE
>Mature_440_residues
MHFLDENIQIKIDKFYKKNSLDKNRVIVAFSGGADSTALLLNLKYYLSNNVIAFYFAHFIRSDNEQNQEIEHVKGFCDLY
NIALQIKKCDIDIKSESARLGVSIEELARKFRYIALENALKENGANYIALAHNENDQIETIIMRFFQGSFLDGLSGIPSV
NRNIIRPLLEVSRLEIENFLSLNNIGFFVDSTNAQNLYLRNRVRNNLLPAIKKVFKGYEKCLKRISEFSKEFADYFGKDE
FFPVEKGKYYYSFDLKTFLDFPKYLVFRLIFKILNSEGIAAKVSYKALNEAFKVEINRKKNNVLLKTNDFFLEKRHNKIN
LIFKRDEKFYKPFDFILEVGKWHSLSLGKILLKYLECNAASVSRLKCCSYEFRYKFFKDRLKAKKFFSKFIRCNPAYLML
LALDNRLIGIIDLNTLNLVWSEKSILKKINISLIGGLLKE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=219, Percent_Identity=25.5707762557078, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 51473; Mature: 51473

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFLDENIQIKIDKFYKKNSLDKNRVIVAFSGGADSTALLLNLKYYLSNNVIAFYFAHFI
CCCCCCCCEEEEEEHHCCCCCCCCEEEEEEECCCCCEEEEEEEEHHHCCCCHHHHHHHHH
RSDNEQNQEIEHVKGFCDLYNIALQIKKCDIDIKSESARLGVSIEELARKFRYIALENAL
HCCCCCCHHHHHHHHHHHHHHHHEEEEEECEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
KENGANYIALAHNENDQIETIIMRFFQGSFLDGLSGIPSVNRNIIRPLLEVSRLEIENFL
HHCCCCEEEEEECCCCHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SLNNIGFFVDSTNAQNLYLRNRVRNNLLPAIKKVFKGYEKCLKRISEFSKEFADYFGKDE
EECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FFPVEKGKYYYSFDLKTFLDFPKYLVFRLIFKILNSEGIAAKVSYKALNEAFKVEINRKK
CEECCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEHHHHCCEEEEEEECCC
NNVLLKTNDFFLEKRHNKINLIFKRDEKFYKPFDFILEVGKWHSLSLGKILLKYLECNAA
CCEEEEECHHHHEECCCEEEEEEECCCCHHCCHHHHHHHCCCCCCHHHHHHHHHHHCCCC
SVSRLKCCSYEFRYKFFKDRLKAKKFFSKFIRCNPAYLMLLALDNRLIGIIDLNTLNLVW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEECCCEEEEEEECEEEEEE
SEKSILKKINISLIGGLLKE
CCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHFLDENIQIKIDKFYKKNSLDKNRVIVAFSGGADSTALLLNLKYYLSNNVIAFYFAHFI
CCCCCCCCEEEEEEHHCCCCCCCCEEEEEEECCCCCEEEEEEEEHHHCCCCHHHHHHHHH
RSDNEQNQEIEHVKGFCDLYNIALQIKKCDIDIKSESARLGVSIEELARKFRYIALENAL
HCCCCCCHHHHHHHHHHHHHHHHEEEEEECEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
KENGANYIALAHNENDQIETIIMRFFQGSFLDGLSGIPSVNRNIIRPLLEVSRLEIENFL
HHCCCCEEEEEECCCCHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SLNNIGFFVDSTNAQNLYLRNRVRNNLLPAIKKVFKGYEKCLKRISEFSKEFADYFGKDE
EECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FFPVEKGKYYYSFDLKTFLDFPKYLVFRLIFKILNSEGIAAKVSYKALNEAFKVEINRKK
CEECCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEHHHHCCEEEEEEECCC
NNVLLKTNDFFLEKRHNKINLIFKRDEKFYKPFDFILEVGKWHSLSLGKILLKYLECNAA
CCEEEEECHHHHEECCCEEEEEEECCCCHHCCHHHHHHHCCCCCCHHHHHHHHHHHCCCC
SVSRLKCCSYEFRYKFFKDRLKAKKFFSKFIRCNPAYLMLLALDNRLIGIIDLNTLNLVW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEECCCEEEEEEECEEEEEE
SEKSILKKINISLIGGLLKE
CCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA