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The map label for this gene is pksG [H]

Identifier: 15595028

GI number: 15595028

Start: 724224

End: 725447

Strand: Direct

Name: pksG [H]

Synonym: BB0683

Alternate gene names: 15595028

Gene position: 724224-725447 (Clockwise)

Preceding gene: 15595026

Following gene: 15595029

Centisome position: 79.52

GC content: 31.21

Gene sequence:

>1224_bases
ATGAGAATAGGTATTAGTGATATTAGAATTTTTTTACCTTTAAATTATTTAGATTTTTCTGTTCTTTTGGAAAATCCTTT
ATATTTTTCTAATGAAGTTTTTTTTAAAAAAATCAATAGAGCAATAGATGCAACCTTGCAAAAAGGTTTTAGGTTTACCA
GTCCTAATGAGGATAGTGTAACCATGGCAAGTTCGGCTGTTAAGCTTATTTTTGACAACAATAATCTTGATTTAAGCAAA
ATTAGAATACTTTTGGGTGGAACTGAAACAGGCGTTGATCATTCAAAGGCCATTTCTTCTTATGTTTTTGGAGCCTTAAA
GCAATCTGGTATTTGTCTGGGAAATAATTTTCTAACTTTCCAGGTTCAACATGCGTGTGCTGGTGCTGCTATGTCTTTGC
ACACTGTGGCAAGTGTTTTAAGCCATTCCAATAATTCTGAATACGGCATAGTTTTTTCTTCAGATATTGCACATTATAGC
AATCTTACTACGGCTGAGATTACCCAAGGAGCCGGCGCAACTGCAATTTTGATTGAAAAAAATCCAAAGCTACTTTCGAT
CAATTTATCTGAATTTGGAGTTTATACTGATGATGTTGACGATTTTTTTAGGCCTTTTGGAAGTGTTGAGGCTAAGGTGC
GAGGTCAGTATTCAGTTGAATGTTACAATAATGCAAACGAAAATGCTTTAAGAGATTTTGCTTTCAAAAAGCAACTTAGT
ATGAAAGATTTATTTTCTAATTATAGGTTTGTTTTGCATGTTCCTTTTGCTAAAATGCCAATAGATTCAATGCATTATAT
TTTGAAAAAATATTATAGCGATGACGAATCTGTTAGAAATGCTTATTTAGAATCAATAGATTTTTACGATGGAGTTGAAG
CTGCTATGGAAGTGGGAAATTTGTATACAGGTTCAATTTTTCTATCTTTAGCATTTTATTTAAAAAGAGTATTTTCCAAG
AAAGATATTACAGGGGAAAAGATATTGTTTTGCTCTTATGGATCTGGCAATATTATGATTATTTATGAACTTACCATCGA
AAAGAGTGCTTTTGATGTTATTAAATTATGGGATCTTGAAGGTCTTATAAAAAATAGAAATAATGCAAATTTTGAAGAAT
ATAAAGATTTTTTTCAAAATAAAATAATTCCTGGTGAATCCAGAGGATTTTATTTAAAAGAACTAAGGAATGATGGATAC
CGAGTTTATGGGTATCGAGCCTAA

Upstream 100 bases:

>100_bases
TTTTCATAAACTTCCATTTTTAGGTTTTATTATATAAGATTTTGCTAATTTTTGTAAATTATAATATAATACTTAAGGCC
ATTAATTCATTGAGGAGTAT

Downstream 100 bases:

>100_bases
TATATTAGAAAATAAAAAAAGGCATATTGAGATTTGTTTAAATAAAAACGATGTTAAAGGTGGCTGTAATTTCTTAAAGT
TTATTAAGCTAAAACACAAT

Product: 3-hydroxy-3-methylglutaryl-CoA synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MRIGISDIRIFLPLNYLDFSVLLENPLYFSNEVFFKKINRAIDATLQKGFRFTSPNEDSVTMASSAVKLIFDNNNLDLSK
IRILLGGTETGVDHSKAISSYVFGALKQSGICLGNNFLTFQVQHACAGAAMSLHTVASVLSHSNNSEYGIVFSSDIAHYS
NLTTAEITQGAGATAILIEKNPKLLSINLSEFGVYTDDVDDFFRPFGSVEAKVRGQYSVECYNNANENALRDFAFKKQLS
MKDLFSNYRFVLHVPFAKMPIDSMHYILKKYYSDDESVRNAYLESIDFYDGVEAAMEVGNLYTGSIFLSLAFYLKRVFSK
KDITGEKILFCSYGSGNIMIIYELTIEKSAFDVIKLWDLEGLIKNRNNANFEEYKDFFQNKIIPGESRGFYLKELRNDGY
RVYGYRA

Sequences:

>Translated_407_residues
MRIGISDIRIFLPLNYLDFSVLLENPLYFSNEVFFKKINRAIDATLQKGFRFTSPNEDSVTMASSAVKLIFDNNNLDLSK
IRILLGGTETGVDHSKAISSYVFGALKQSGICLGNNFLTFQVQHACAGAAMSLHTVASVLSHSNNSEYGIVFSSDIAHYS
NLTTAEITQGAGATAILIEKNPKLLSINLSEFGVYTDDVDDFFRPFGSVEAKVRGQYSVECYNNANENALRDFAFKKQLS
MKDLFSNYRFVLHVPFAKMPIDSMHYILKKYYSDDESVRNAYLESIDFYDGVEAAMEVGNLYTGSIFLSLAFYLKRVFSK
KDITGEKILFCSYGSGNIMIIYELTIEKSAFDVIKLWDLEGLIKNRNNANFEEYKDFFQNKIIPGESRGFYLKELRNDGY
RVYGYRA
>Mature_407_residues
MRIGISDIRIFLPLNYLDFSVLLENPLYFSNEVFFKKINRAIDATLQKGFRFTSPNEDSVTMASSAVKLIFDNNNLDLSK
IRILLGGTETGVDHSKAISSYVFGALKQSGICLGNNFLTFQVQHACAGAAMSLHTVASVLSHSNNSEYGIVFSSDIAHYS
NLTTAEITQGAGATAILIEKNPKLLSINLSEFGVYTDDVDDFFRPFGSVEAKVRGQYSVECYNNANENALRDFAFKKQLS
MKDLFSNYRFVLHVPFAKMPIDSMHYILKKYYSDDESVRNAYLESIDFYDGVEAAMEVGNLYTGSIFLSLAFYLKRVFSK
KDITGEKILFCSYGSGNIMIIYELTIEKSAFDVIKLWDLEGLIKNRNNANFEEYKDFFQNKIIPGESRGFYLKELRNDGY
RVYGYRA

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI5031751, Length=468, Percent_Identity=24.7863247863248, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI148298764, Length=413, Percent_Identity=25.90799031477, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI148298677, Length=413, Percent_Identity=25.90799031477, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560126, Length=414, Percent_Identity=25.3623188405797, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323509, Length=422, Percent_Identity=23.9336492890995, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24654139, Length=423, Percent_Identity=25.7683215130024, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24654137, Length=423, Percent_Identity=25.7683215130024, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24654133, Length=423, Percent_Identity=25.7683215130024, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24654135, Length=423, Percent_Identity=25.7683215130024, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17933694, Length=423, Percent_Identity=25.7683215130024, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 46030; Mature: 46030

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGISDIRIFLPLNYLDFSVLLENPLYFSNEVFFKKINRAIDATLQKGFRFTSPNEDSV
CCCCCCEEEEEEEECHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCE
TMASSAVKLIFDNNNLDLSKIRILLGGTETGVDHSKAISSYVFGALKQSGICLGNNFLTF
EEECCEEEEEEECCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEECCCEEEE
QVQHACAGAAMSLHTVASVLSHSNNSEYGIVFSSDIAHYSNLTTAEITQGAGATAILIEK
EEECHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCHHHEECCCCCEEEEEEC
NPKLLSINLSEFGVYTDDVDDFFRPFGSVEAKVRGQYSVECYNNANENALRDFAFKKQLS
CCCEEEEEHHHCCCCCCCHHHHHCCCCCCEEEECCEEEEEEECCCCHHHHHHHHHHHHCC
MKDLFSNYRFVLHVPFAKMPIDSMHYILKKYYSDDESVRNAYLESIDFYDGVEAAMEVGN
HHHHHCCCEEEEECCHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCC
LYTGSIFLSLAFYLKRVFSKKDITGEKILFCSYGSGNIMIIYELTIEKSAFDVIKLWDLE
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCEEEEECHH
GLIKNRNNANFEEYKDFFQNKIIPGESRGFYLKELRNDGYRVYGYRA
HHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MRIGISDIRIFLPLNYLDFSVLLENPLYFSNEVFFKKINRAIDATLQKGFRFTSPNEDSV
CCCCCCEEEEEEEECHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCE
TMASSAVKLIFDNNNLDLSKIRILLGGTETGVDHSKAISSYVFGALKQSGICLGNNFLTF
EEECCEEEEEEECCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEECCCEEEE
QVQHACAGAAMSLHTVASVLSHSNNSEYGIVFSSDIAHYSNLTTAEITQGAGATAILIEK
EEECHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCHHHEECCCCCEEEEEEC
NPKLLSINLSEFGVYTDDVDDFFRPFGSVEAKVRGQYSVECYNNANENALRDFAFKKQLS
CCCEEEEEHHHCCCCCCCHHHHHCCCCCCEEEECCEEEEEEECCCCHHHHHHHHHHHHCC
MKDLFSNYRFVLHVPFAKMPIDSMHYILKKYYSDDESVRNAYLESIDFYDGVEAAMEVGN
HHHHHCCCEEEEECCHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCC
LYTGSIFLSLAFYLKRVFSKKDITGEKILFCSYGSGNIMIIYELTIEKSAFDVIKLWDLE
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCEEEEECHH
GLIKNRNNANFEEYKDFFQNKIIPGESRGFYLKELRNDGYRVYGYRA
HHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]