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The map label for this gene is ptsH

Identifier: 15594902

GI number: 15594902

Start: 569278

End: 569538

Strand: Direct

Name: ptsH

Synonym: BB0557

Alternate gene names: 15594902

Gene position: 569278-569538 (Clockwise)

Preceding gene: 15594901

Following gene: 15594903

Centisome position: 62.51

GC content: 36.4

Gene sequence:

>261_bases
ATGGTAAAAAAAGAAGCAATTATTAAGGCTGTAAACGGTTTGCATGTTAGGCCTGCATCAACTTTTGTAAAAAAAGCTAA
AGAGTATTCTAGCGAGATAACAATAGAATCTGATGGAAAGTCTGTTAGCGGAAAAAGTTTGTTCAGGCTTCAAACTTTGG
AATTATCATCAGGTAAAAAGCTTTTGATATGTGCTGAGGGTGAGGATGAAGAGATTGCTGCTTCAGAGCTTGCAGAGCTT
ATCGAATCTTTTAAGGAATGA

Upstream 100 bases:

>100_bases
TATTTCTTATGTAAAGGGAATATATAATTTATTTTTATGTATTATAATGTAAAAAGTTAATCAAAAAAAACAATATTCAT
AATGAGGGGTTTCTGTACTT

Downstream 100 bases:

>100_bases
ATTTTAGAAGGATTTAAGTTATGACTTTATCGGGCAAAAGAATATCCAAAGGGATAGGCATTGGGGAAGTTCTTTGTATT
AGGAAAAATTTTGATAAAAT

Product: phosphocarrier protein HPr

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; D-glucosamine-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; galactitol-1-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm]; fructose-1-phosphate [Cytoplasm]; glucose-6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm]; salicin-6-phosphate [Cytoplasm]; arbutin-6-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein

Number of amino acids: Translated: 86; Mature: 86

Protein sequence:

>86_residues
MVKKEAIIKAVNGLHVRPASTFVKKAKEYSSEITIESDGKSVSGKSLFRLQTLELSSGKKLLICAEGEDEEIAASELAEL
IESFKE

Sequences:

>Translated_86_residues
MVKKEAIIKAVNGLHVRPASTFVKKAKEYSSEITIESDGKSVSGKSLFRLQTLELSSGKKLLICAEGEDEEIAASELAEL
IESFKE
>Mature_86_residues
MVKKEAIIKAVNGLHVRPASTFVKKAKEYSSEITIESDGKSVSGKSLFRLQTLELSSGKKLLICAEGEDEEIAASELAEL
IESFKE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain

Homologues:

Organism=Escherichia coli, GI1788755, Length=81, Percent_Identity=55.5555555555556, Blast_Score=93, Evalue=3e-21,

Paralogues:

None

Copy number: 4180 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 2100 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 3235 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PTHP_BORBU (O51507)

Other databases:

- EMBL:   AE000783
- PIR:   D70169
- RefSeq:   NP_212691.1
- ProteinModelPortal:   O51507
- SMR:   O51507
- EnsemblBacteria:   EBBORT00000008425
- GeneID:   1195404
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0557
- TIGR:   BB_0557
- GeneTree:   EBGT00050000006797
- HOGENOM:   HBG653254
- OMA:   YASDITI
- PhylomeDB:   O51507
- ProtClustDB:   CLSK507662
- BioCyc:   BBUR224326:BB_0557-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- Gene3D:   G3DSA:3.30.1340.10
- PRINTS:   PR00107
- TIGRFAMs:   TIGR01003

Pfam domain/function: PF00381 PTS-HPr; SSF55594 HPr_protein

EC number: NA

Molecular weight: Translated: 9386; Mature: 9386

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS51350 PTS_HPR_DOM; PS00369 PTS_HPR_HIS; PS00589 PTS_HPR_SER

Important sites: ACT_SITE 15-15

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKEAIIKAVNGLHVRPASTFVKKAKEYSSEITIESDGKSVSGKSLFRLQTLELSSGKK
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEEEEECCCCCE
LLICAEGEDEEIAASELAELIESFKE
EEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKKEAIIKAVNGLHVRPASTFVKKAKEYSSEITIESDGKSVSGKSLFRLQTLELSSGKK
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEEEEECCCCCE
LLICAEGEDEEIAASELAELIESFKE
EEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sorbitol [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm]; mannitol [Periplasm]; glucosamine [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; galactitol [Periplasm]; fructose [Periplasm]; beta-D-glucose [Periplasm]; diacetylchitobiose [Periplasm]; cellobiose [Periplasm]; salicin [Periplasm]; arbutin [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + mannitol [Periplasm] = mannitol-1-phosp

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685