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The map label for this gene is leuS [H]

Identifier: 15594596

GI number: 15594596

Start: 256463

End: 258985

Strand: Direct

Name: leuS [H]

Synonym: BB0251

Alternate gene names: 15594596

Gene position: 256463-258985 (Clockwise)

Preceding gene: 15594595

Following gene: 15594597

Centisome position: 28.16

GC content: 31.83

Gene sequence:

>2523_bases
ATGTCTAAATACGAATTCATAAAAATTGAAAAAAAATGGCAAGAATTTTGGGATAATAACAAAACATACAAAGTAGAAGA
AGATCCAAGTATTCCCAAGGAAAAAAGATTGTATATACTTGACATGTTCCCCTATCCTTCTGCCAACGGGCTTCATGTTG
GTCATCCAGAAGGATACACAGCAACAGACATATTTGGAAGATACAAGCTTTTGAATGGATTTCATGTACTTCACCCAATA
GGATTTGATAGCTTTGGACTTCCTGCTGAAAATTACGCAATACAAACAGGAACTCACCCTCAAAAAAGTACAGAAGAAAA
TATTAATAAGTTTAAAAAACAAATAAAAGCTTTGGGGTTTGCCTATGATTGGGATCGAGAAATTAGAACACATGAGGAGA
ATTACTATAAATGGACACAGTGGATTTTCCTGCAATTATATAAAAAAGGGCTAGCTTATGTAAAAGAAATGCCTGTATGG
TACTGCCCCGAGCTTGGAACGGTACTAGCAAATGAAGAGATTATTCAAACTTCAGATGGACCAAAATCTGAGAGAGGATC
TTACAGCGTCGAAAAAAAATATTTAAGACAGTGGGTTTTAAAAATTACAAAGTATGCTGAAAGATTGCTAGACGACCTTG
AAGAATTAGAATGGCCTGAATCTGTAAAAGAAATGCAGCGAAATTGGATTGGCAAATCAACAGGAGTTGAAATTGAATTT
GAAATTGAAGGCCACAGCGATAAAATTAAAGTCTTTACAACAAGGCCAGACACAATCTTTGGCATCACATATTTAGTGAT
CGCACCAGAAAATAAACTAATAGAAAAAATAACAAAGAACAACTTTAAACAAAATGTTTTAAAATATGTAAAGCACGAAG
AACTCAAAAGCGATCTTAATAGGACTTCTCTTGAAAAAGATAAATCGGGAGTTTTCACAGGATCTTACGCATTTCATCCA
ATAACAAATGAAAAAATTCCAATTTGGGTTGGAAGCTACGTACTAGGAACTTACGGAACCGGAGCTGTAATGGGTGTTCC
AGCACATGATGAAAGAGACTTTCAATTTGCCAAAAAGTATCAATTAAAAATTTTACCCGTGATATCAAAATCGGGGAAAA
ATGAAATATTAGAAAAAGCATTTGTTGATGATGGAATTTCAATAAATTCTCCCAATGAATTTAATAATCTTAAAAATTCT
GAAGTAAAAGACAAAGTAATAAAATGGCTTACTAAAAACAAAAAAGGGAAAGAAAAAGTTGCCTACAAGCTTAGAGATTG
GATTTTTTCAAGACAAAGATACTGGGGAGAGCCTATACCCATTTTGTTTGATAAACTTGGAAATGCAATACCTTTAGAGG
AAAATGATCTTCCCTTAAAGCTTCCAGAAATTGCAAACTATAAACCTTCTGGAACAGGTGAATCTCCTTTGTCAAGAATT
AAAGATTGGGTAAACGTAAAAGATATGGGCTTTACAAGAGAAACAAACACAATGCCTCAATGGGCAGGCTCTTGTTGGTA
TTATTTAAGATACTTAGACCCCAAAAACTCAAAAGAGTTTGCAAACAAAAAAAAAATTGAATATTGGATGCCGGTTGATC
TATACATAGGCGGAGCTGAACATACAGTATTACACCTGCTTTACTCAAGGTTTTGGCACAAGGTTCTTTATGATCTAGGA
TACGTAAATACCAAAGAACCTTTTAAAAAGCTAATAAATCAAGGCATAATAACATCATTTTCTTATCAAAAAGAAAACGG
GGTTTTAATACCCAATGACCAAGTTATAGAAAAAGACAATAAATTTTTTGATAAAAAAGATAACAAAGAAGTAACCCAAG
TAATTGCCAAAATGTCAAAATCTTTAAAAAATGTAATAAACCCAGACGACATTATTAAGGAATTTGGAGCAGACTCAATG
AGAATTTATGAAATGTTTATGGGGCCACTAACAGATTCTAAGCCTTGGAACACTAAAGGAATTATTGGTGTTTTTCGATT
TTTAAACAAAATTTGGAACTTAAGAGAAAAAGAACTATCAAAAGAGAATCCCCCAAGGGAAATAATATCTGAACTACATA
AAGTAATAAAAAAAGTCACAGAAGACACAGAAAAACTAAATTTTAACACAGCAATCTCCGCCATGATGATATTTATAAAT
GAACTTCTAAAGTATGAAAAAAATTATTTAAACATATTTAAACCGTTTATCATTATTTTATCTCCCTATGCACCCCATTT
AGCAGAAGAGTTGTGGGAATATATTGGAGAGCTTCCTAGTTTATTCAAAAATTCAAAATGGCCAAAATTCGATGAAAGCC
TTATTATTAAAGGCAAAAAAGAAATTGTCTTGCAAATAAATGGAAAAATAAAAGACAAAATTTTACTAAACAAAGAGACA
GGCGAAAAAGAACTAAAAGAAATAGCAATGGAAAATAGCAAAATAAAATCAAACTTATTAAATAAAAAAATAGTAAAAAT
AATTGTAATTAAAAACAAGCTTGTCAACATAGTGATAAAGTAA

Upstream 100 bases:

>100_bases
CATCAAAATTGTTAGCAAATTATTAAATAAAATTAACAAAAATGCAATCTAAAAGAATATTAGAGAATAAGTTGTTATGA
GTTAATTTTCAAGAGGACAA

Downstream 100 bases:

>100_bases
AAAAGAGGCGTTATATGGACTTTGAAGGTCTAAGCATTAGATATAAAGGAATTATATTCACAATATTTATCTTAATTACT
ATTTTTTTAGGATTTTTTTT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 840; Mature: 839

Protein sequence:

>840_residues
MSKYEFIKIEKKWQEFWDNNKTYKVEEDPSIPKEKRLYILDMFPYPSANGLHVGHPEGYTATDIFGRYKLLNGFHVLHPI
GFDSFGLPAENYAIQTGTHPQKSTEENINKFKKQIKALGFAYDWDREIRTHEENYYKWTQWIFLQLYKKGLAYVKEMPVW
YCPELGTVLANEEIIQTSDGPKSERGSYSVEKKYLRQWVLKITKYAERLLDDLEELEWPESVKEMQRNWIGKSTGVEIEF
EIEGHSDKIKVFTTRPDTIFGITYLVIAPENKLIEKITKNNFKQNVLKYVKHEELKSDLNRTSLEKDKSGVFTGSYAFHP
ITNEKIPIWVGSYVLGTYGTGAVMGVPAHDERDFQFAKKYQLKILPVISKSGKNEILEKAFVDDGISINSPNEFNNLKNS
EVKDKVIKWLTKNKKGKEKVAYKLRDWIFSRQRYWGEPIPILFDKLGNAIPLEENDLPLKLPEIANYKPSGTGESPLSRI
KDWVNVKDMGFTRETNTMPQWAGSCWYYLRYLDPKNSKEFANKKKIEYWMPVDLYIGGAEHTVLHLLYSRFWHKVLYDLG
YVNTKEPFKKLINQGIITSFSYQKENGVLIPNDQVIEKDNKFFDKKDNKEVTQVIAKMSKSLKNVINPDDIIKEFGADSM
RIYEMFMGPLTDSKPWNTKGIIGVFRFLNKIWNLREKELSKENPPREIISELHKVIKKVTEDTEKLNFNTAISAMMIFIN
ELLKYEKNYLNIFKPFIIILSPYAPHLAEELWEYIGELPSLFKNSKWPKFDESLIIKGKKEIVLQINGKIKDKILLNKET
GEKELKEIAMENSKIKSNLLNKKIVKIIVIKNKLVNIVIK

Sequences:

>Translated_840_residues
MSKYEFIKIEKKWQEFWDNNKTYKVEEDPSIPKEKRLYILDMFPYPSANGLHVGHPEGYTATDIFGRYKLLNGFHVLHPI
GFDSFGLPAENYAIQTGTHPQKSTEENINKFKKQIKALGFAYDWDREIRTHEENYYKWTQWIFLQLYKKGLAYVKEMPVW
YCPELGTVLANEEIIQTSDGPKSERGSYSVEKKYLRQWVLKITKYAERLLDDLEELEWPESVKEMQRNWIGKSTGVEIEF
EIEGHSDKIKVFTTRPDTIFGITYLVIAPENKLIEKITKNNFKQNVLKYVKHEELKSDLNRTSLEKDKSGVFTGSYAFHP
ITNEKIPIWVGSYVLGTYGTGAVMGVPAHDERDFQFAKKYQLKILPVISKSGKNEILEKAFVDDGISINSPNEFNNLKNS
EVKDKVIKWLTKNKKGKEKVAYKLRDWIFSRQRYWGEPIPILFDKLGNAIPLEENDLPLKLPEIANYKPSGTGESPLSRI
KDWVNVKDMGFTRETNTMPQWAGSCWYYLRYLDPKNSKEFANKKKIEYWMPVDLYIGGAEHTVLHLLYSRFWHKVLYDLG
YVNTKEPFKKLINQGIITSFSYQKENGVLIPNDQVIEKDNKFFDKKDNKEVTQVIAKMSKSLKNVINPDDIIKEFGADSM
RIYEMFMGPLTDSKPWNTKGIIGVFRFLNKIWNLREKELSKENPPREIISELHKVIKKVTEDTEKLNFNTAISAMMIFIN
ELLKYEKNYLNIFKPFIIILSPYAPHLAEELWEYIGELPSLFKNSKWPKFDESLIIKGKKEIVLQINGKIKDKILLNKET
GEKELKEIAMENSKIKSNLLNKKIVKIIVIKNKLVNIVIK
>Mature_839_residues
SKYEFIKIEKKWQEFWDNNKTYKVEEDPSIPKEKRLYILDMFPYPSANGLHVGHPEGYTATDIFGRYKLLNGFHVLHPIG
FDSFGLPAENYAIQTGTHPQKSTEENINKFKKQIKALGFAYDWDREIRTHEENYYKWTQWIFLQLYKKGLAYVKEMPVWY
CPELGTVLANEEIIQTSDGPKSERGSYSVEKKYLRQWVLKITKYAERLLDDLEELEWPESVKEMQRNWIGKSTGVEIEFE
IEGHSDKIKVFTTRPDTIFGITYLVIAPENKLIEKITKNNFKQNVLKYVKHEELKSDLNRTSLEKDKSGVFTGSYAFHPI
TNEKIPIWVGSYVLGTYGTGAVMGVPAHDERDFQFAKKYQLKILPVISKSGKNEILEKAFVDDGISINSPNEFNNLKNSE
VKDKVIKWLTKNKKGKEKVAYKLRDWIFSRQRYWGEPIPILFDKLGNAIPLEENDLPLKLPEIANYKPSGTGESPLSRIK
DWVNVKDMGFTRETNTMPQWAGSCWYYLRYLDPKNSKEFANKKKIEYWMPVDLYIGGAEHTVLHLLYSRFWHKVLYDLGY
VNTKEPFKKLINQGIITSFSYQKENGVLIPNDQVIEKDNKFFDKKDNKEVTQVIAKMSKSLKNVINPDDIIKEFGADSMR
IYEMFMGPLTDSKPWNTKGIIGVFRFLNKIWNLREKELSKENPPREIISELHKVIKKVTEDTEKLNFNTAISAMMIFINE
LLKYEKNYLNIFKPFIIILSPYAPHLAEELWEYIGELPSLFKNSKWPKFDESLIIKGKKEIVLQINGKIKDKILLNKETG
EKELKEIAMENSKIKSNLLNKKIVKIIVIKNKLVNIVIK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=882, Percent_Identity=30.952380952381, Blast_Score=440, Evalue=1e-123,
Organism=Escherichia coli, GI1786861, Length=872, Percent_Identity=38.5321100917431, Blast_Score=597, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI71997510, Length=778, Percent_Identity=29.9485861182519, Blast_Score=290, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI71997517, Length=778, Percent_Identity=29.9485861182519, Blast_Score=290, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI212645227, Length=315, Percent_Identity=31.4285714285714, Blast_Score=142, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323414, Length=892, Percent_Identity=33.8565022421525, Blast_Score=443, Evalue=1e-125,
Organism=Drosophila melanogaster, GI21355409, Length=841, Percent_Identity=30.7966706302021, Blast_Score=365, Evalue=1e-100,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 98159; Mature: 98028

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYEFIKIEKKWQEFWDNNKTYKVEEDPSIPKEKRLYILDMFPYPSANGLHVGHPEGYT
CCCCEEEEHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCEECCCCCCCC
ATDIFGRYKLLNGFHVLHPIGFDSFGLPAENYAIQTGTHPQKSTEENINKFKKQIKALGF
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCC
AYDWDREIRTHEENYYKWTQWIFLQLYKKGLAYVKEMPVWYCPELGTVLANEEIIQTSDG
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHCCCCCEECCCC
PKSERGSYSVEKKYLRQWVLKITKYAERLLDDLEELEWPESVKEMQRNWIGKSTGVEIEF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEE
EIEGHSDKIKVFTTRPDTIFGITYLVIAPENKLIEKITKNNFKQNVLKYVKHEELKSDLN
EECCCCCEEEEEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTSLEKDKSGVFTGSYAFHPITNEKIPIWVGSYVLGTYGTGAVMGVPAHDERDFQFAKKY
HHHHHHCCCCEEECCEEECCCCCCCCEEEECCCHHCCCCCCCEEECCCCCCCCHHHHHHC
QLKILPVISKSGKNEILEKAFVDDGISINSPNEFNNLKNSEVKDKVIKWLTKNKKGKEKV
CEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCHHHH
AYKLRDWIFSRQRYWGEPIPILFDKLGNAIPLEENDLPLKLPEIANYKPSGTGESPLSRI
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEECCHHCCCCCCCCCCCHHHHH
KDWVNVKDMGFTRETNTMPQWAGSCWYYLRYLDPKNSKEFANKKKIEYWMPVDLYIGGAE
HHHCCHHHCCCCCCCCCCCHHHCCCEEEEEEECCCCCHHHHCCCCEEEEECEEEEECCCH
HTVLHLLYSRFWHKVLYDLGYVNTKEPFKKLINQGIITSFSYQKENGVLIPNDQVIEKDN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECEECCCCEECCCCHHHCCCC
KFFDKKDNKEVTQVIAKMSKSLKNVINPDDIIKEFGADSMRIYEMFMGPLTDSKPWNTKG
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCH
IIGVFRFLNKIWNLREKELSKENPPREIISELHKVIKKVTEDTEKLNFNTAISAMMIFIN
HHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ELLKYEKNYLNIFKPFIIILSPYAPHLAEELWEYIGELPSLFKNSKWPKFDESLIIKGKK
HHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCE
EIVLQINGKIKDKILLNKETGEKELKEIAMENSKIKSNLLNKKIVKIIVIKNKLVNIVIK
EEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEEEC
>Mature Secondary Structure 
SKYEFIKIEKKWQEFWDNNKTYKVEEDPSIPKEKRLYILDMFPYPSANGLHVGHPEGYT
CCCEEEEHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCEECCCCCCCC
ATDIFGRYKLLNGFHVLHPIGFDSFGLPAENYAIQTGTHPQKSTEENINKFKKQIKALGF
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCC
AYDWDREIRTHEENYYKWTQWIFLQLYKKGLAYVKEMPVWYCPELGTVLANEEIIQTSDG
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHCCCCCEECCCC
PKSERGSYSVEKKYLRQWVLKITKYAERLLDDLEELEWPESVKEMQRNWIGKSTGVEIEF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEE
EIEGHSDKIKVFTTRPDTIFGITYLVIAPENKLIEKITKNNFKQNVLKYVKHEELKSDLN
EECCCCCEEEEEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTSLEKDKSGVFTGSYAFHPITNEKIPIWVGSYVLGTYGTGAVMGVPAHDERDFQFAKKY
HHHHHHCCCCEEECCEEECCCCCCCCEEEECCCHHCCCCCCCEEECCCCCCCCHHHHHHC
QLKILPVISKSGKNEILEKAFVDDGISINSPNEFNNLKNSEVKDKVIKWLTKNKKGKEKV
CEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCHHHH
AYKLRDWIFSRQRYWGEPIPILFDKLGNAIPLEENDLPLKLPEIANYKPSGTGESPLSRI
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEECCHHCCCCCCCCCCCHHHHH
KDWVNVKDMGFTRETNTMPQWAGSCWYYLRYLDPKNSKEFANKKKIEYWMPVDLYIGGAE
HHHCCHHHCCCCCCCCCCCHHHCCCEEEEEEECCCCCHHHHCCCCEEEEECEEEEECCCH
HTVLHLLYSRFWHKVLYDLGYVNTKEPFKKLINQGIITSFSYQKENGVLIPNDQVIEKDN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECEECCCCEECCCCHHHCCCC
KFFDKKDNKEVTQVIAKMSKSLKNVINPDDIIKEFGADSMRIYEMFMGPLTDSKPWNTKG
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCH
IIGVFRFLNKIWNLREKELSKENPPREIISELHKVIKKVTEDTEKLNFNTAISAMMIFIN
HHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ELLKYEKNYLNIFKPFIIILSPYAPHLAEELWEYIGELPSLFKNSKWPKFDESLIIKGKK
HHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCE
EIVLQINGKIKDKILLNKETGEKELKEIAMENSKIKSNLLNKKIVKIIVIKNKLVNIVIK
EEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA