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The map label for this gene is ahpF [H]

Identifier: 154248141

GI number: 154248141

Start: 4674219

End: 4675808

Strand: Direct

Name: ahpF [H]

Synonym: Xaut_4221

Alternate gene names: 154248141

Gene position: 4674219-4675808 (Clockwise)

Preceding gene: 154248140

Following gene: 154248142

Centisome position: 88.04

GC content: 66.73

Gene sequence:

>1590_bases
ATGCTCGACACAGCCATGAAGACTCAGCTCAAGGCGTATCTGGAGCGGCTCGTCCGACCCGTCGAAATCACCGCCTTCGT
GGATGACGGCGCCAAGTCGAAGGAGATGCTGGAGCTGCTCTCCGACATCACCGACTGCTCGCCCAAGGTCACCGTCGCCG
AGCGCCGCGACGGCGATGCTGCCGGCGCCCGCGTGCCCTCCTTCGCCCTGTCCAGCCCCGGCGAGAGCATCGCGCTCACC
TTCGCGGGCCTGCCCATGGGCCATGAATTTACTTCGCTGGTGCTGGCGCTGCTGCAGACCGGCGGCTACCCGCCCAAGCT
GGAAGCGGAGGTCATCGAGCAGATCAAGGCGCTGGACGGCGACTTCGTCTTCGAGACCTATTTTTCCCAGTCCTGCCAGA
ACTGCCCGGATGTGGTGCAGGCCCTCAACCTCATGGCCGTGCTGAACCCGAAGATCCGCCACGTGGCCATCGACGGCGCC
CTGTTCCAGAAGGAAGTGGACGCCCGGCAGGTCATGTCGGTGCCCACCGTGTACCTGAACGGCGAAGTGTTCGGCCAGGG
CCGCATGGGCGTGGAAGAGATCGTCGCCAAGCTCGACACCGGTGCAGCCGCCCGCGATGCCAAGCGCATGGCCGAGATGA
ACCGCTTCGACGTGCTGGTGGTGGGCGGTGGCCCGGCCGGCGCCACGGCGGCGATCTATGCGGCACGCAAGGGCATCCGC
ACCGGCGTCGCCGCCGAGCGTTTCGGCGGCCAGGTGCTGGACACCATGGCCATCGAGAACTTCATCTCCGTGCCCTACAC
CGAGGGCCCCAAGCTCGCCGCCGCGCTGGAGCAGCATGTGCGCGAGTATGAGGTGGAGATCCTCAACCTGCAGAAGGCGG
TGAAGCTGGTGCCCGGCGCCGACATCGTCGAGGTGGAATTCGCCAACGGCGCGAGCCTGCGCTCCAAGACGGTGATTCTC
GCCACCGGCGCCCGCTGGCGGCAGATGAACGTGCCGGGCGAGGACACCTATCGCAACAAGGGCGTGGCCTATTGCCCCCA
TTGCGACGGCCCGCTGTTCAAGGGCAAGCGCGTGGCGGTGATCGGCGGCGGCAATTCCGGCGTGGAAGCGGCCATCGATC
TGGCCGGCATCGTTGCCCACGTGACGCTGATGGAGTTCGACAGCGCCTTGCGCGCCGACGACGTGCTCCAGCGCAAGCTG
AAGAGCCTGCCCAACGTCACCATCCTCACCTCGGCCAAGACCACCGAAGTGCTGGGCGACGGCCAGAAGGTGAGCGGCCT
CACCTACGAGAACCGCAACACCGGCGAGAGCGTGAAGGTGGAGCTGGAAGGCATTTTCGTGCAGATCGGCCTTCTGCCCA
ACACCGAGTGGCTGAAGGGCACTGTGGAGCTGTCTCCCCGCGGCGAGATCGTGGTGGATGCCCACGGCCAGACCTCGGTG
CCCGGCGTCTTCGCCGCCGGCGACTGCACCACGGTGCCCTACAAGCAGATCGTGATCGCGCTGGGCGAAGGCGCCAAGGC
CTCGCTCTCCGCCTTCGATCACCTCATCCGCTCCAGCGCGCCGAAGGAAGAGGCGGTGAAGGCCGCGTGA

Upstream 100 bases:

>100_bases
TCCTTCCATTGGGGAGGACCCACCTCCTCCGCCGCGCGTTCCGCGCGCGGCGGATGCGGAGCACCTTCTCGTTTTACAAA
GCATTCGTCGGAGAATTGCC

Downstream 100 bases:

>100_bases
CCGGCCGGGCACGCCACTTCCTCTTTCTCCGGGCGACCTCCCATGGGGGCCGCCCGCGGTGACCCTGCGCGAACTACAAT
ATCTCGTCGCCCTGGCCGAC

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MLDTAMKTQLKAYLERLVRPVEITAFVDDGAKSKEMLELLSDITDCSPKVTVAERRDGDAAGARVPSFALSSPGESIALT
FAGLPMGHEFTSLVLALLQTGGYPPKLEAEVIEQIKALDGDFVFETYFSQSCQNCPDVVQALNLMAVLNPKIRHVAIDGA
LFQKEVDARQVMSVPTVYLNGEVFGQGRMGVEEIVAKLDTGAAARDAKRMAEMNRFDVLVVGGGPAGATAAIYAARKGIR
TGVAAERFGGQVLDTMAIENFISVPYTEGPKLAAALEQHVREYEVEILNLQKAVKLVPGADIVEVEFANGASLRSKTVIL
ATGARWRQMNVPGEDTYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLMEFDSALRADDVLQRKL
KSLPNVTILTSAKTTEVLGDGQKVSGLTYENRNTGESVKVELEGIFVQIGLLPNTEWLKGTVELSPRGEIVVDAHGQTSV
PGVFAAGDCTTVPYKQIVIALGEGAKASLSAFDHLIRSSAPKEEAVKAA

Sequences:

>Translated_529_residues
MLDTAMKTQLKAYLERLVRPVEITAFVDDGAKSKEMLELLSDITDCSPKVTVAERRDGDAAGARVPSFALSSPGESIALT
FAGLPMGHEFTSLVLALLQTGGYPPKLEAEVIEQIKALDGDFVFETYFSQSCQNCPDVVQALNLMAVLNPKIRHVAIDGA
LFQKEVDARQVMSVPTVYLNGEVFGQGRMGVEEIVAKLDTGAAARDAKRMAEMNRFDVLVVGGGPAGATAAIYAARKGIR
TGVAAERFGGQVLDTMAIENFISVPYTEGPKLAAALEQHVREYEVEILNLQKAVKLVPGADIVEVEFANGASLRSKTVIL
ATGARWRQMNVPGEDTYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLMEFDSALRADDVLQRKL
KSLPNVTILTSAKTTEVLGDGQKVSGLTYENRNTGESVKVELEGIFVQIGLLPNTEWLKGTVELSPRGEIVVDAHGQTSV
PGVFAAGDCTTVPYKQIVIALGEGAKASLSAFDHLIRSSAPKEEAVKAA
>Mature_529_residues
MLDTAMKTQLKAYLERLVRPVEITAFVDDGAKSKEMLELLSDITDCSPKVTVAERRDGDAAGARVPSFALSSPGESIALT
FAGLPMGHEFTSLVLALLQTGGYPPKLEAEVIEQIKALDGDFVFETYFSQSCQNCPDVVQALNLMAVLNPKIRHVAIDGA
LFQKEVDARQVMSVPTVYLNGEVFGQGRMGVEEIVAKLDTGAAARDAKRMAEMNRFDVLVVGGGPAGATAAIYAARKGIR
TGVAAERFGGQVLDTMAIENFISVPYTEGPKLAAALEQHVREYEVEILNLQKAVKLVPGADIVEVEFANGASLRSKTVIL
ATGARWRQMNVPGEDTYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLMEFDSALRADDVLQRKL
KSLPNVTILTSAKTTEVLGDGQKVSGLTYENRNTGESVKVELEGIFVQIGLLPNTEWLKGTVELSPRGEIVVDAHGQTSV
PGVFAAGDCTTVPYKQIVIALGEGAKASLSAFDHLIRSSAPKEEAVKAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=521, Percent_Identity=63.915547024952, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=323, Percent_Identity=33.4365325077399, Blast_Score=161, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6321898, Length=340, Percent_Identity=32.9411764705882, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320560, Length=313, Percent_Identity=34.5047923322684, Blast_Score=155, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56401; Mature: 56401

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTAMKTQLKAYLERLVRPVEITAFVDDGAKSKEMLELLSDITDCSPKVTVAERRDGDA
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
AGARVPSFALSSPGESIALTFAGLPMGHEFTSLVLALLQTGGYPPKLEAEVIEQIKALDG
CCCCCCCHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
DFVFETYFSQSCQNCPDVVQALNLMAVLNPKIRHVAIDGALFQKEVDARQVMSVPTVYLN
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEC
GEVFGQGRMGVEEIVAKLDTGAAARDAKRMAEMNRFDVLVVGGGPAGATAAIYAARKGIR
CEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
TGVAAERFGGQVLDTMAIENFISVPYTEGPKLAAALEQHVREYEVEILNLQKAVKLVPGA
CCCHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DIVEVEFANGASLRSKTVILATGARWRQMNVPGEDTYRNKGVAYCPHCDGPLFKGKRVAV
CEEEEEECCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCEEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAGIVAHVTLMEFDSALRADDVLQRKLKSLPNVTILTSAKTTEVLGD
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCC
GQKVSGLTYENRNTGESVKVELEGIFVQIGLLPNTEWLKGTVELSPRGEIVVDAHGQTSV
CCEECCCEECCCCCCCEEEEEEEEEEEEEEECCCCHHHEEEEEECCCCCEEEECCCCCCC
PGVFAAGDCTTVPYKQIVIALGEGAKASLSAFDHLIRSSAPKEEAVKAA
CCEEECCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
MLDTAMKTQLKAYLERLVRPVEITAFVDDGAKSKEMLELLSDITDCSPKVTVAERRDGDA
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
AGARVPSFALSSPGESIALTFAGLPMGHEFTSLVLALLQTGGYPPKLEAEVIEQIKALDG
CCCCCCCHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
DFVFETYFSQSCQNCPDVVQALNLMAVLNPKIRHVAIDGALFQKEVDARQVMSVPTVYLN
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEC
GEVFGQGRMGVEEIVAKLDTGAAARDAKRMAEMNRFDVLVVGGGPAGATAAIYAARKGIR
CEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
TGVAAERFGGQVLDTMAIENFISVPYTEGPKLAAALEQHVREYEVEILNLQKAVKLVPGA
CCCHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DIVEVEFANGASLRSKTVILATGARWRQMNVPGEDTYRNKGVAYCPHCDGPLFKGKRVAV
CEEEEEECCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCEEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAGIVAHVTLMEFDSALRADDVLQRKLKSLPNVTILTSAKTTEVLGD
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCC
GQKVSGLTYENRNTGESVKVELEGIFVQIGLLPNTEWLKGTVELSPRGEIVVDAHGQTSV
CCEECCCEECCCCCCCEEEEEEEEEEEEEEECCCCHHHEEEEEECCCCCEEEECCCCCCC
PGVFAAGDCTTVPYKQIVIALGEGAKASLSAFDHLIRSSAPKEEAVKAA
CCEEECCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]