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The map label for this gene is rsmE [H]

Identifier: 154248025

GI number: 154248025

Start: 4540594

End: 4541322

Strand: Direct

Name: rsmE [H]

Synonym: Xaut_4104

Alternate gene names: 154248025

Gene position: 4540594-4541322 (Clockwise)

Preceding gene: 154248024

Following gene: 154248026

Centisome position: 85.53

GC content: 71.6

Gene sequence:

>729_bases
ATGCCCGATCATGATTTCCGCGCCCAGCGCCTCTACGTGGACGCAGAGCTCGGGCCGGACGCGTCCGTCCTGCTCGCGCC
CGAGCAGGCCCATTACGTCGCCAACGTGATCCGGCTGGCCGTGGGCCAGGACCTGCTGCTGTTCAATGGGCGCGATGGCG
AGTGGCGCGCCACCCGTGTCGCCGGCGGCAAGCGCGACGTGCGCTTCGAGGTGAGCGCTCAAGTGCGCCCGCAACCGGAG
GCGCCGACGCTGGAATATGCCTTCGCGCCCCTGAAGCACGCCCGCCTCGACTACATGGTGCAGAAAGCGGTGGAGATGGG
CGCCGGCGTGCTCTCTCCCGTCATCACCCGCTACACCCAGGTGTCGCGCATCAATCTCGATCGCATGGCAGCCAACGCCG
TGGAGGCGGCCGAGCAATGCGGCATTCTCAGCCTGCCCCACGTCGCCGAGCCGCGCCCGCTTGCCCAATGGCTCGACGCG
CTGCCGGCCGAGCGGGTGCTCGTCTTCTGCGACGAGGCGGCGGCCGACCATGATCCCATCCATGCCCTGCGCACGGCCCC
TTCCGGGCCGGTGAGCGTGCTGATCGGCCCGGAAGGCGGCTTCAGCCCGGACGAGCGCGCCCTGATCGCCCCGCGCGCGG
TCACGCTCTCCCTCGGCCCGCGCATTCTGCGGGCGGATACGGCGGCGGTGGCCGCCCTGGCCTTGGTGGGGGCGGCGCGG
GGTGGCTGA

Upstream 100 bases:

>100_bases
AGTCATCGCCCATCTGCCATCGCCCGGCTGTCCGCTCGCGGTCGAGCTTGCCAAGTGCCGGCCTGTCGTGGTGTCCCTCC
CGCACCGGAGGAGGACCCCC

Downstream 100 bases:

>100_bases
ATGGCGCTATAAACGCCCGAATGGCCGTTTTGTTCCCATTCATCTTGGAAGAATTCGTCACACATGCTGAAACACTTATA
AACAAGTGATTCGTAATAGG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MPDHDFRAQRLYVDAELGPDASVLLAPEQAHYVANVIRLAVGQDLLLFNGRDGEWRATRVAGGKRDVRFEVSAQVRPQPE
APTLEYAFAPLKHARLDYMVQKAVEMGAGVLSPVITRYTQVSRINLDRMAANAVEAAEQCGILSLPHVAEPRPLAQWLDA
LPAERVLVFCDEAAADHDPIHALRTAPSGPVSVLIGPEGGFSPDERALIAPRAVTLSLGPRILRADTAAVAALALVGAAR
GG

Sequences:

>Translated_242_residues
MPDHDFRAQRLYVDAELGPDASVLLAPEQAHYVANVIRLAVGQDLLLFNGRDGEWRATRVAGGKRDVRFEVSAQVRPQPE
APTLEYAFAPLKHARLDYMVQKAVEMGAGVLSPVITRYTQVSRINLDRMAANAVEAAEQCGILSLPHVAEPRPLAQWLDA
LPAERVLVFCDEAAADHDPIHALRTAPSGPVSVLIGPEGGFSPDERALIAPRAVTLSLGPRILRADTAAVAALALVGAAR
GG
>Mature_241_residues
PDHDFRAQRLYVDAELGPDASVLLAPEQAHYVANVIRLAVGQDLLLFNGRDGEWRATRVAGGKRDVRFEVSAQVRPQPEA
PTLEYAFAPLKHARLDYMVQKAVEMGAGVLSPVITRYTQVSRINLDRMAANAVEAAEQCGILSLPHVAEPRPLAQWLDAL
PAERVLVFCDEAAADHDPIHALRTAPSGPVSVLIGPEGGFSPDERALIAPRAVTLSLGPRILRADTAAVAALALVGAARG
G

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=237, Percent_Identity=27.4261603375527, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25913; Mature: 25782

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDHDFRAQRLYVDAELGPDASVLLAPEQAHYVANVIRLAVGQDLLLFNGRDGEWRATRV
CCCCCCCEEEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEE
AGGKRDVRFEVSAQVRPQPEAPTLEYAFAPLKHARLDYMVQKAVEMGAGVLSPVITRYTQ
CCCCCEEEEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VSRINLDRMAANAVEAAEQCGILSLPHVAEPRPLAQWLDALPAERVLVFCDEAAADHDPI
HHHCCHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCH
HALRTAPSGPVSVLIGPEGGFSPDERALIAPRAVTLSLGPRILRADTAAVAALALVGAAR
HHHHCCCCCCEEEEECCCCCCCCCCCEEECCCEEEEECCCEEEHHHHHHHHHHHHHHHCC
GG
CC
>Mature Secondary Structure 
PDHDFRAQRLYVDAELGPDASVLLAPEQAHYVANVIRLAVGQDLLLFNGRDGEWRATRV
CCCCCCEEEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEE
AGGKRDVRFEVSAQVRPQPEAPTLEYAFAPLKHARLDYMVQKAVEMGAGVLSPVITRYTQ
CCCCCEEEEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VSRINLDRMAANAVEAAEQCGILSLPHVAEPRPLAQWLDALPAERVLVFCDEAAADHDPI
HHHCCHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCH
HALRTAPSGPVSVLIGPEGGFSPDERALIAPRAVTLSLGPRILRADTAAVAALALVGAAR
HHHHCCCCCCEEEEECCCCCCCCCCCEEECCCEEEEECCCEEEHHHHHHHHHHHHHHHCC
GG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]