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The map label for this gene is braE [H]

Identifier: 154247824

GI number: 154247824

Start: 4318403

End: 4319920

Strand: Direct

Name: braE [H]

Synonym: Xaut_3901

Alternate gene names: 154247824

Gene position: 4318403-4319920 (Clockwise)

Preceding gene: 154247823

Following gene: 154247825

Centisome position: 81.34

GC content: 67.59

Gene sequence:

>1518_bases
ATGGCGCATCCGCCCGTCGCCGACCTGAAGCCGGAGGTCCTGCCCAAGGGCGATCTTCCGGCCCCCGGCCTGCCAGGCGC
TCCGACCCTTCCACCGCCCACTCCGCACCAGGGGGCGGCCTCCGTCATCGGCAGCGCATTTACGGATGCGATCGTGAGCG
GCCTGCTCACGGGCCTGCTGCTGCTGCCGCTGGTGGGCTTCCGCGCCACGGAGTCGGGCAGCGGGCCGCTGGGCCTCACC
TATCGCTTCGGTCTCGTGGCCGTGTTTGCCGGCATCGTCGCGGTGCTGCGGCTGGTGCTGAACCTCACGGTCTGGCGGCG
CGGCCGCAAGGTGAAGGTGAGCGCTGGGCCTTCCGCCTTCACCACGCTTGCGCAGAAGGCGGCGCCGGCCCTGAGGCCGG
CCTTCTTCAGCTTCGCCATCGCCTATCCCTTCCTTGCCATCCTGCTGGCGGGGGGCCTGAGCCCCAGCCGCTACTGGGTG
GACCTCGGAATCTATGTGCTCACCTATGTGATGCTGGGCTGGGGCCTCAACATCGTGGTGGGCCTCGCCGGGCTGCTCGA
TCTCGGCTATGTGGCCTTCTACGCCGTGGGCGCCTACACCTTCGCCCTGCTCTCCACCAGCGTGCCGGTGAACGACTTCT
TCGCCGGCCATCTCGGGGAGGGGTTCTGGACCGCCTGGTCGTTCTGGCTGTGCCTGCCCGTGTCCGGGCTGCTGGCCGCC
TTTTGGGGCGTCATCCTGGGCTTCCCGGTGCTGCGGTTGAGGGGCGACTATCTCGCCATCGTCACGCTGGCGTTCGGCGA
GATCATCCGCCTCGTGCTCATCAACTGGGTGTCGCTCACCAACGGCGGGGCGGGCATCTCCTCCATCCCGCCCATCTCCT
TTTTCGGCGTGCCGTTCAACGCCACCGACGAAGGCTTCGCGGCGAAGTATGGGCTCGACTTCGATTCCATGCACCGGATC
ATCTTCCTGTATTTCGTGATCCTGGGGCTGGCCGCGCTGACCGCGCTGGTCACCATCCGGCTGCGCCAGATGCCGGTGGG
CCGGGCCTGGGAGGCCCTGCGGGAGGACGAGATCGCCTGCCGCTCGCTGGGCATCAACACCACCCTCACCAAGCTCACCG
CGTTTGCCACCGGCGCCATGTTCGGCGGTTTCGCCGGTGCCTTCTTCGCGGTGCGGGTGCGCTTCGTCTCGCCCGAGAGC
TTCACCTTTATGGAATCGGCGGTGATCCTCGCCATCGTGGTGCTGGGCGGCATGGGATCGCAGATGGGGGTGGCGGGGGC
GGCCATCCTGCTCATCGGCGGCATGGAGATGCTGCGCAACCTCTCCTTCCTGAAAGCCGACTTCCTGTTCGGGCCGGATT
TCGACCCTTCGCTCTACCGCATGCTCATCTTCGGCTTCGCCATGGTGGCAGTAATGGTGTGGCGCCCGCGCGGCCTCGTC
TCCTCGCGCCTGCCCACCATCTTCCTGAAGGAACGCAAGGCGGTCTCCTCGGCCCTCGTCAAGGAGGGCCACGGCTGA

Upstream 100 bases:

>100_bases
CCATTGAATACAAGGATGTGGCAGCGTTCTCCATCCTTGTGGTCACGCTCATCTTCCTGCCCCAGGGCCTGCTGGGCCGG
CCGGAAGTGGAGAAGGTCTG

Downstream 100 bases:

>100_bases
TGGCGAACCTTGATCCCGTGGCCGGCGCACGGGCGCCGATCCTGGCGGTGGACCACCTGTCCATGCGCTTTGGCGGCCTG
ATCGCCGTGAATGACGTGTC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MAHPPVADLKPEVLPKGDLPAPGLPGAPTLPPPTPHQGAASVIGSAFTDAIVSGLLTGLLLLPLVGFRATESGSGPLGLT
YRFGLVAVFAGIVAVLRLVLNLTVWRRGRKVKVSAGPSAFTTLAQKAAPALRPAFFSFAIAYPFLAILLAGGLSPSRYWV
DLGIYVLTYVMLGWGLNIVVGLAGLLDLGYVAFYAVGAYTFALLSTSVPVNDFFAGHLGEGFWTAWSFWLCLPVSGLLAA
FWGVILGFPVLRLRGDYLAIVTLAFGEIIRLVLINWVSLTNGGAGISSIPPISFFGVPFNATDEGFAAKYGLDFDSMHRI
IFLYFVILGLAALTALVTIRLRQMPVGRAWEALREDEIACRSLGINTTLTKLTAFATGAMFGGFAGAFFAVRVRFVSPES
FTFMESAVILAIVVLGGMGSQMGVAGAAILLIGGMEMLRNLSFLKADFLFGPDFDPSLYRMLIFGFAMVAVMVWRPRGLV
SSRLPTIFLKERKAVSSALVKEGHG

Sequences:

>Translated_505_residues
MAHPPVADLKPEVLPKGDLPAPGLPGAPTLPPPTPHQGAASVIGSAFTDAIVSGLLTGLLLLPLVGFRATESGSGPLGLT
YRFGLVAVFAGIVAVLRLVLNLTVWRRGRKVKVSAGPSAFTTLAQKAAPALRPAFFSFAIAYPFLAILLAGGLSPSRYWV
DLGIYVLTYVMLGWGLNIVVGLAGLLDLGYVAFYAVGAYTFALLSTSVPVNDFFAGHLGEGFWTAWSFWLCLPVSGLLAA
FWGVILGFPVLRLRGDYLAIVTLAFGEIIRLVLINWVSLTNGGAGISSIPPISFFGVPFNATDEGFAAKYGLDFDSMHRI
IFLYFVILGLAALTALVTIRLRQMPVGRAWEALREDEIACRSLGINTTLTKLTAFATGAMFGGFAGAFFAVRVRFVSPES
FTFMESAVILAIVVLGGMGSQMGVAGAAILLIGGMEMLRNLSFLKADFLFGPDFDPSLYRMLIFGFAMVAVMVWRPRGLV
SSRLPTIFLKERKAVSSALVKEGHG
>Mature_504_residues
AHPPVADLKPEVLPKGDLPAPGLPGAPTLPPPTPHQGAASVIGSAFTDAIVSGLLTGLLLLPLVGFRATESGSGPLGLTY
RFGLVAVFAGIVAVLRLVLNLTVWRRGRKVKVSAGPSAFTTLAQKAAPALRPAFFSFAIAYPFLAILLAGGLSPSRYWVD
LGIYVLTYVMLGWGLNIVVGLAGLLDLGYVAFYAVGAYTFALLSTSVPVNDFFAGHLGEGFWTAWSFWLCLPVSGLLAAF
WGVILGFPVLRLRGDYLAIVTLAFGEIIRLVLINWVSLTNGGAGISSIPPISFFGVPFNATDEGFAAKYGLDFDSMHRII
FLYFVILGLAALTALVTIRLRQMPVGRAWEALREDEIACRSLGINTTLTKLTAFATGAMFGGFAGAFFAVRVRFVSPESF
TFMESAVILAIVVLGGMGSQMGVAGAAILLIGGMEMLRNLSFLKADFLFGPDFDPSLYRMLIFGFAMVAVMVWRPRGLVS
SRLPTIFLKERKAVSSALVKEGHG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=344, Percent_Identity=52.9069767441861, Blast_Score=291, Evalue=6e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 53797; Mature: 53666

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AHPPVADLKPEVLPKGDLPAPGLPGAPTLPPPTPHQGAASVIGSAFTDAIVSGLLTGLL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH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HHCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]