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The map label for this gene is ksgA

Identifier: 154246772

GI number: 154246772

Start: 3159929

End: 3160795

Strand: Reverse

Name: ksgA

Synonym: Xaut_2833

Alternate gene names: 154246772

Gene position: 3160795-3159929 (Counterclockwise)

Preceding gene: 154246773

Following gene: 154246768

Centisome position: 59.54

GC content: 70.13

Gene sequence:

>867_bases
GTGAGCGCCCTCGACGACCTGCCGCCGCTGCGCGATGTCATCCGGCGGCACGGCCTCTCGGCGCAGAAGTCGCTGGGCCA
GAACTTCCTTCTGGACCTCAACCTCACCGGCCGCATCGCCCGCGCCTCCGGCCCCCTCGAAGGGGCCACCGTGGTGGAGG
TGGGTCCCGGACCGGGCGGGCTGACGCGGGCGCTGCTCGCTTTGGGTGCCCGGCGCGTCATCGCCATCGAGCGCGATCAG
CGCTGCCTCGATGCGCTGGCCGAAGTCTCCGACCATTATCCCGGCCGGCTGGAGGTGATCTCCGGCGATGCGCTGAAGGT
GGACGTTCGCCCCCTCGTGGGTGACGGTGAGGTGCGGGTGGTGGCGAACCTGCCCTACAATATTGCGACGCTGCTGCTCA
TCGGCTGGCTCTCAACGGATCCCTGGCCGCCGTGGTTCTCGTCCCTCACCCTCATGTTCCAGAAGGAAGTGGCGGAGCGC
ATCGTCGCCGCGCCGGGCTCGAAAGCCTATGGCCGGCTCGCGGTGCTGGCGGGCTGGCGGACCACGGCGCGCATCGCCTT
CGACGTGGCCCCCTCCGCCTTCGTGCCACCGCCGAAGGTGACCTCCTCCGTGGTGCATCTGGTGCCGCGGTCGGAGCCTT
TGCCCTGCGCCTTGTCGGCCCTGGAGAAGGTGACGGAAGCCGCCTTCGGCCAGCGCCGCAAGATGCTGCGCCAGAGCCTG
AAATCCCTGGGGGTCGACACCGCCGCCCTGCTCAAAGCCACTGGCGTGGAGGAAACGGCGCGGGCCGAGGAGATCGATGT
GGACGGCTTCGTCCGGCTGGCGAACACGTTCGCGCAGCTGAAGTCGGGAGCCGGCGCGGCGGCGTGA

Upstream 100 bases:

>100_bases
ACCCCTCAAGCCTGGTGGCGGCGCTGCGGCTCGCCCGCCGCCTCGCGGATGCGGAAGCGCGGCTTGCCGCGCGCGAAGGC
CACCGCGCGGCGAGTGTAGC

Downstream 100 bases:

>100_bases
GGCCGCCATCGCCCCTCGGCACCTCGCACGGAGTGGCCGGCCGCCGCGACCGGGACGGCCGGGACTGACGGAGGCGCCCC
CTACTCCGCCGCGGCCACCT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSALDDLPPLRDVIRRHGLSAQKSLGQNFLLDLNLTGRIARASGPLEGATVVEVGPGPGGLTRALLALGARRVIAIERDQ
RCLDALAEVSDHYPGRLEVISGDALKVDVRPLVGDGEVRVVANLPYNIATLLLIGWLSTDPWPPWFSSLTLMFQKEVAER
IVAAPGSKAYGRLAVLAGWRTTARIAFDVAPSAFVPPPKVTSSVVHLVPRSEPLPCALSALEKVTEAAFGQRRKMLRQSL
KSLGVDTAALLKATGVEETARAEEIDVDGFVRLANTFAQLKSGAGAAA

Sequences:

>Translated_288_residues
MSALDDLPPLRDVIRRHGLSAQKSLGQNFLLDLNLTGRIARASGPLEGATVVEVGPGPGGLTRALLALGARRVIAIERDQ
RCLDALAEVSDHYPGRLEVISGDALKVDVRPLVGDGEVRVVANLPYNIATLLLIGWLSTDPWPPWFSSLTLMFQKEVAER
IVAAPGSKAYGRLAVLAGWRTTARIAFDVAPSAFVPPPKVTSSVVHLVPRSEPLPCALSALEKVTEAAFGQRRKMLRQSL
KSLGVDTAALLKATGVEETARAEEIDVDGFVRLANTFAQLKSGAGAAA
>Mature_287_residues
SALDDLPPLRDVIRRHGLSAQKSLGQNFLLDLNLTGRIARASGPLEGATVVEVGPGPGGLTRALLALGARRVIAIERDQR
CLDALAEVSDHYPGRLEVISGDALKVDVRPLVGDGEVRVVANLPYNIATLLLIGWLSTDPWPPWFSSLTLMFQKEVAERI
VAAPGSKAYGRLAVLAGWRTTARIAFDVAPSAFVPPPKVTSSVVHLVPRSEPLPCALSALEKVTEAAFGQRRKMLRQSLK
SLGVDTAALLKATGVEETARAEEIDVDGFVRLANTFAQLKSGAGAAA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=296, Percent_Identity=36.1486486486487, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI7657198, Length=289, Percent_Identity=30.4498269896194, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1786236, Length=269, Percent_Identity=37.546468401487, Blast_Score=151, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25141369, Length=305, Percent_Identity=32.4590163934426, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25146882, Length=216, Percent_Identity=35.6481481481481, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=202, Percent_Identity=34.6534653465347, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21357273, Length=293, Percent_Identity=32.4232081911263, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21358017, Length=263, Percent_Identity=33.0798479087452, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_XANP2 (A7IJ80)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001417730.1
- ProteinModelPortal:   A7IJ80
- SMR:   A7IJ80
- STRING:   A7IJ80
- GeneID:   5423083
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_2833
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   XAUT78245:XAUT_2833-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30637; Mature: 30506

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 28-28 BINDING 30-30 BINDING 55-55 BINDING 77-77 BINDING 103-103 BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALDDLPPLRDVIRRHGLSAQKSLGQNFLLDLNLTGRIARASGPLEGATVVEVGPGPGG
CCCCCCCCHHHHHHHHCCCHHHHHCCCCEEEEECCCCEEEECCCCCCCCEEEEECCCCCH
LTRALLALGARRVIAIERDQRCLDALAEVSDHYPGRLEVISGDALKVDVRPLVGDGEVRV
HHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEEECCCCEEE
VANLPYNIATLLLIGWLSTDPWPPWFSSLTLMFQKEVAERIVAAPGSKAYGRLAVLAGWR
EEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCEEEEEECCC
TTARIAFDVAPSAFVPPPKVTSSVVHLVPRSEPLPCALSALEKVTEAAFGQRRKMLRQSL
CEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KSLGVDTAALLKATGVEETARAEEIDVDGFVRLANTFAQLKSGAGAAA
HHCCCCHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SALDDLPPLRDVIRRHGLSAQKSLGQNFLLDLNLTGRIARASGPLEGATVVEVGPGPGG
CCCCCCCHHHHHHHHCCCHHHHHCCCCEEEEECCCCEEEECCCCCCCCEEEEECCCCCH
LTRALLALGARRVIAIERDQRCLDALAEVSDHYPGRLEVISGDALKVDVRPLVGDGEVRV
HHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEEECCCCEEE
VANLPYNIATLLLIGWLSTDPWPPWFSSLTLMFQKEVAERIVAAPGSKAYGRLAVLAGWR
EEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCEEEEEECCC
TTARIAFDVAPSAFVPPPKVTSSVVHLVPRSEPLPCALSALEKVTEAAFGQRRKMLRQSL
CEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KSLGVDTAALLKATGVEETARAEEIDVDGFVRLANTFAQLKSGAGAAA
HHCCCCHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA