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The map label for this gene is cysG [H]

Identifier: 154246317

GI number: 154246317

Start: 2651788

End: 2653311

Strand: Reverse

Name: cysG [H]

Synonym: Xaut_2375

Alternate gene names: 154246317

Gene position: 2653311-2651788 (Counterclockwise)

Preceding gene: 154246318

Following gene: 154246315

Centisome position: 49.98

GC content: 73.36

Gene sequence:

>1524_bases
ATGGCCGCCACCGGCCCCGACACCCCCAACTCCGGCGCCCTGTCGCCCCGCGCCCCGCGCCAGGCCGGACATGCGCGCAT
GGAGGCCCTCGCGCGCCTGCCGGTGTTCTTTGGCCTGGAGGGCCGGCGCGTGCTGGTGGCGGGCGGCGGCGAGCCGGCGG
CCTGGAAGGCGGAACTGCTCTCCGCCGCCGGGGCGCGGGTGGAGGTGATCGCCCCCCATGTCTGCGAGGACCTGTGGCGG
CTGGCCGGCGCGCCGCCCCGTGGACCCATCTCGATCCATGTGCGCGACTGGCACGCCGACGACATCCCCGGCGCCGTCCT
CGCGGTGGCCGGCTTTGACGAAGAGGCGGACGCCCTCAACTTCACCACCGCCGCCCGCGCTGCCGGCGTGCCGGTGAACG
CCATCGACAAGCCGGCCCTCTCCGACTTCACCTTCGGCGCCATCGTGAACCGCTCGCCGCTGGTGGTGGGCATCTCCACC
GACGGCGCGGCGCCCGTCTTCGGGCAGGCGGTGCGGGCCAAGATCGAGGCGCTGTTGCCCTCGGGCTTCAAGGCCTGGGC
GCTGGCCGCAAAGACGTGGCGGCAGGCGGTGACCGCGCGCGGCCTCTCCCACCACGCCCGCCGCGCCTTCTGGGAACGCT
TCGCCGCCACGGCGCTGGCCGAGCCCAACCGGGCGCCGCAGGACACCGACCGCGCCGCCCTGTTCACCGCCATCGAGGCG
GAGGGCACGCCGCACCGCTCCGGCCACGCGGGCTCGGTGGTGCTGGTGGGCGCAGGCCCCGGCGATCCGGAGCTGCTCAC
GCTCAAGGCGGTGCGGGCGCTGCAATCGGCGGACGTGGTGCTCTATGACGACCTCGTCTCCGGCGCGATCCTCGATTTCG
CCCGGCGCGAGGCGAAGAAGATGCTGGTGGGCAAGACCGGCTACGGCCCCTCCTGCAAGCAGGACGACATCAACGCTTTG
ATGGTGGACCTCGCCAAGGAAGGCCGCCGCGTGGTGCGCCTGAAGGGCGGCGAGCCCATGATCTTCGGCCGCGCCGGGGA
AGAGATCACCCATTGCCATGCGGCGGGGGTGCCGGTGGAGGTGATCCCCGGCATCTCGGCACCGCAGGGGGCGGCGAGCC
GGCTGGTCACCTCGCTCACCCACCGCGACCATGCACGGCGGCTGCAATTCGTCACCGCCCATGCTCGCGACGGCAAGCTG
CCCAAGGACCTCGACTTCAACGCGCTGGCCGACAAGGCGGCGACGACGGTGGTCTACATGCCCCGCCGCACCCTGCCGGA
CCTGGTGCGCAACCTGATGGCCGCGGGCATCGATCCGGCGACGCCCGCGGTGGCCATGTTCTCGGTGACGCGGCCGGAGG
AGAAGGTGATCCTCGCCACCGTCGCGACGCTGGCGGAAGAGGTGGCCCGCGCCGCCGAGGTGGGCGCGCAAGGCCCCTGC
CTCATCCTCTACGGCCAGGCCCTGGCCGAAGTGGCGGTGGATGCCCTGCCCCAGATCGTCCAGGCTGCCGCCAACGGCGG
CTGA

Upstream 100 bases:

>100_bases
GCGGCGCCCCGCGCGTCCGCCGGAGGGCCGCAGCCGCGTTGATCGGCGGACCGGCACGCCTTACGTTGAAGATCCAACAG
TTTCGGGAGCACCTTTCCCC

Downstream 100 bases:

>100_bases
GGGGCGAATCCCGACGCCAGCCCGCGTTGCCCCCTTCAGGAATACGTCCCAAACACACTCTCGTCATGCCCCGGCTTGAC
CGGGGCATCCACGCCGCCGG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MAATGPDTPNSGALSPRAPRQAGHARMEALARLPVFFGLEGRRVLVAGGGEPAAWKAELLSAAGARVEVIAPHVCEDLWR
LAGAPPRGPISIHVRDWHADDIPGAVLAVAGFDEEADALNFTTAARAAGVPVNAIDKPALSDFTFGAIVNRSPLVVGIST
DGAAPVFGQAVRAKIEALLPSGFKAWALAAKTWRQAVTARGLSHHARRAFWERFAATALAEPNRAPQDTDRAALFTAIEA
EGTPHRSGHAGSVVLVGAGPGDPELLTLKAVRALQSADVVLYDDLVSGAILDFARREAKKMLVGKTGYGPSCKQDDINAL
MVDLAKEGRRVVRLKGGEPMIFGRAGEEITHCHAAGVPVEVIPGISAPQGAASRLVTSLTHRDHARRLQFVTAHARDGKL
PKDLDFNALADKAATTVVYMPRRTLPDLVRNLMAAGIDPATPAVAMFSVTRPEEKVILATVATLAEEVARAAEVGAQGPC
LILYGQALAEVAVDALPQIVQAAANGG

Sequences:

>Translated_507_residues
MAATGPDTPNSGALSPRAPRQAGHARMEALARLPVFFGLEGRRVLVAGGGEPAAWKAELLSAAGARVEVIAPHVCEDLWR
LAGAPPRGPISIHVRDWHADDIPGAVLAVAGFDEEADALNFTTAARAAGVPVNAIDKPALSDFTFGAIVNRSPLVVGIST
DGAAPVFGQAVRAKIEALLPSGFKAWALAAKTWRQAVTARGLSHHARRAFWERFAATALAEPNRAPQDTDRAALFTAIEA
EGTPHRSGHAGSVVLVGAGPGDPELLTLKAVRALQSADVVLYDDLVSGAILDFARREAKKMLVGKTGYGPSCKQDDINAL
MVDLAKEGRRVVRLKGGEPMIFGRAGEEITHCHAAGVPVEVIPGISAPQGAASRLVTSLTHRDHARRLQFVTAHARDGKL
PKDLDFNALADKAATTVVYMPRRTLPDLVRNLMAAGIDPATPAVAMFSVTRPEEKVILATVATLAEEVARAAEVGAQGPC
LILYGQALAEVAVDALPQIVQAAANGG
>Mature_506_residues
AATGPDTPNSGALSPRAPRQAGHARMEALARLPVFFGLEGRRVLVAGGGEPAAWKAELLSAAGARVEVIAPHVCEDLWRL
AGAPPRGPISIHVRDWHADDIPGAVLAVAGFDEEADALNFTTAARAAGVPVNAIDKPALSDFTFGAIVNRSPLVVGISTD
GAAPVFGQAVRAKIEALLPSGFKAWALAAKTWRQAVTARGLSHHARRAFWERFAATALAEPNRAPQDTDRAALFTAIEAE
GTPHRSGHAGSVVLVGAGPGDPELLTLKAVRALQSADVVLYDDLVSGAILDFARREAKKMLVGKTGYGPSCKQDDINALM
VDLAKEGRRVVRLKGGEPMIFGRAGEEITHCHAAGVPVEVIPGISAPQGAASRLVTSLTHRDHARRLQFVTAHARDGKLP
KDLDFNALADKAATTVVYMPRRTLPDLVRNLMAAGIDPATPAVAMFSVTRPEEKVILATVATLAEEVARAAEVGAQGPCL
ILYGQALAEVAVDALPQIVQAAANGG

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=466, Percent_Identity=35.1931330472103, Blast_Score=242, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6322922, Length=238, Percent_Identity=31.0924369747899, Blast_Score=98, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 53051; Mature: 52920

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATGPDTPNSGALSPRAPRQAGHARMEALARLPVFFGLEGRRVLVAGGGEPAAWKAELL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCEEEEECCCCCCHHHHHHH
SAAGARVEVIAPHVCEDLWRLAGAPPRGPISIHVRDWHADDIPGAVLAVAGFDEEADALN
HHCCCEEEEECHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCC
FTTAARAAGVPVNAIDKPALSDFTFGAIVNRSPLVVGISTDGAAPVFGQAVRAKIEALLP
HHHHHHHCCCCCCCCCCCCCCCCCHHHEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
SGFKAWALAAKTWRQAVTARGLSHHARRAFWERFAATALAEPNRAPQDTDRAALFTAIEA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEC
EGTPHRSGHAGSVVLVGAGPGDPELLTLKAVRALQSADVVLYDDLVSGAILDFARREAKK
CCCCCCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHH
MLVGKTGYGPSCKQDDINALMVDLAKEGRRVVRLKGGEPMIFGRAGEEITHCHAAGVPVE
HHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCHHHHHHHCCCCEE
VIPGISAPQGAASRLVTSLTHRDHARRLQFVTAHARDGKLPKDLDFNALADKAATTVVYM
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHCEEEEEE
PRRTLPDLVRNLMAAGIDPATPAVAMFSVTRPEEKVILATVATLAEEVARAAEVGAQGPC
CCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
LILYGQALAEVAVDALPQIVQAAANGG
EEEEHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AATGPDTPNSGALSPRAPRQAGHARMEALARLPVFFGLEGRRVLVAGGGEPAAWKAELL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCEEEEECCCCCCHHHHHHH
SAAGARVEVIAPHVCEDLWRLAGAPPRGPISIHVRDWHADDIPGAVLAVAGFDEEADALN
HHCCCEEEEECHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCC
FTTAARAAGVPVNAIDKPALSDFTFGAIVNRSPLVVGISTDGAAPVFGQAVRAKIEALLP
HHHHHHHCCCCCCCCCCCCCCCCCHHHEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
SGFKAWALAAKTWRQAVTARGLSHHARRAFWERFAATALAEPNRAPQDTDRAALFTAIEA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEC
EGTPHRSGHAGSVVLVGAGPGDPELLTLKAVRALQSADVVLYDDLVSGAILDFARREAKK
CCCCCCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHH
MLVGKTGYGPSCKQDDINALMVDLAKEGRRVVRLKGGEPMIFGRAGEEITHCHAAGVPVE
HHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCHHHHHHHCCCCEE
VIPGISAPQGAASRLVTSLTHRDHARRLQFVTAHARDGKLPKDLDFNALADKAATTVVYM
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHCEEEEEE
PRRTLPDLVRNLMAAGIDPATPAVAMFSVTRPEEKVILATVATLAEEVARAAEVGAQGPC
CCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
LILYGQALAEVAVDALPQIVQAAANGG
EEEEHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA