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The map label for this gene is gcl [H]

Identifier: 154246309

GI number: 154246309

Start: 2641879

End: 2643654

Strand: Direct

Name: gcl [H]

Synonym: Xaut_2367

Alternate gene names: 154246309

Gene position: 2641879-2643654 (Clockwise)

Preceding gene: 154246306

Following gene: 154246310

Centisome position: 49.76

GC content: 67.23

Gene sequence:

>1776_bases
ATGGCCCGCATGCGTGCTGTCGATGCCGCCGTCCGTGTCCTTGAGAAGGAAGGCATCCATTGCGCCTTCGGCGTTCCGGG
CGCAGCCATCAACCCGCTCTATTCCGCCATGAAGGCGCGGGGCTCCATCCGCCACGTGCTGGCGCGCCACGTGGAGGGCG
CCTCCCACATGGCCGAGGGCTACACCCGCGCGGTGGCCGGCAATATCGGCGTGTGCATCGGCACCTCCGGCCCCGCCGGC
ACCGACATGATCACCGGCCTCTACTCGGCCTCGGCGGATTCGATCCCGATCCTGTGCATCACCGGCCAGGCCCCCCGCGC
CCGCCTCTACAAGGAAGACTTCCAGGCGGTGGACATCGAATCCATCGCCAAGCCCGTCACCAAGTGGGCGGTCACCGTGC
GCGAGCCCGCGCTGGTGCCCATGGTGTTCCAGCAGGCGTTCCACGTCATGCGCTCCGGCCGTCCCGGCCCGGTGCTCATC
GACCTGCCCATCGACGTGCAGCTCGCCGAGATCGAGTTCGACGAGGAGACCTACGCCAGCCTGCCGGTCTACAAGCCGGC
GGCGACCCAGGCGCAGATCGACAAGGCGATGGAGATGATCGTCGGCGCCAAGCGCCCGCTCATCGTCTCCGGCGGCGGCG
TCATCAATGCCGATGCCTCGGACCTGCTGGTGGAATTCGCCGAGCTGACCGGCATCCCGGTCATCCCCACCCTCATGGGA
TGGGGCACCATCCCCGACGACCATCCGCTGATGGCCGGCATGTGCGGCCTGCAGACCTCGCACCGCTACGGCAACGCCAA
CATGCTGGCGTCCGACTTCGTGGTCGGCATCGGCAACCGCTGGGCCAACCGCCACACCGGCTCGGTGGAGGTCTATACGG
AGGGGCGCAAGTTCATCCACGTGGACATCGAGCCCACCCAGATCGGCCGCGTGTTCGGGCCGGACTTCGGCATCGTGTCC
GACGCCAAGGCGGCGCTGGAGCTGTTCGTTGTCGCCGCGAAGGCGCTCAAGGCCGCCGGCAAGCTGCCGGACTGGTCCAC
GTGGGCCAACGAATGCCTGGAGCGCAAGCAGACCATGCTGCGCAAGTCCCACTTCGACGCGGTGCCGCTGAAGCCCCAGC
GGGTCTATGAGGAGATGAACGAGGCGCTCGGCCGCGACGTCACCTATGTGACCACCATCGGCCTGTCGCAGATCGCCGGC
GCGCAGTTCCTGCACGTCTACAATCCGCGCCACTGGATCAATTGCGGCCAGGCCGGCCCGCTCGGCTGGACCCTGCCGGC
CGCCCTCGGCGTGCGCGCCGCGCGGCCCGATGCCAAGATCGTGGCGCTCTCGGGCGACTACGATTTCCAGTTCATGGTGG
AAGAACTGGCAGTGGGCGCCCAGCACAAGCTGCCCTACCTGCACGTGGTGGTGAACAACTCCTACCTCGGCCTCATCCGG
CAGGCGCAGCGCGGCTTCAACATGGACTTCGAGGTGAGCCTCGCCTTCGACAACATCAATTCGGAGGAGGCCGGCGCCTA
CGGCGTGGACCATGTGGCGGTGGCCGAAGGCCTCGGCTGCAAGGCCATCCGCGTGCGCACCCCCAACGAGTTCAAGGACG
CCTTCGCCAACGCCCAGAAGCTCATGGACGAGCATCAGGTGCCGGTGGTGGTCGAGTTCATCCTGGAGCGCGTCACCAAC
ATCGCCATGGGCCTCGAGATCAACGCGGTTAACGAGTTCGAGGAAATCCTCGACGTGCCGCTGGATCAGGTCCGCGCCCT
GGAGCCGGCGGAGTAA

Upstream 100 bases:

>100_bases
CTCTCAGGGCATCAATGTCATTCCGTTTCTTCCCATAGAAGCGAAGACGGGCCGGGCGTATCCAGTATGCAAGTCAAAAA
GACCAATAGGAGGAAAAGCC

Downstream 100 bases:

>100_bases
AACCCGCACGCTCCGCTCCCGGCATCTGCGGCATCCCCCGGCTTCTCCGGGGGGTGCCCTTTGCCGCCCCTCGCCCCTTT
CCAAAAAGCCGTGGATGCCC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MARMRAVDAAVRVLEKEGIHCAFGVPGAAINPLYSAMKARGSIRHVLARHVEGASHMAEGYTRAVAGNIGVCIGTSGPAG
TDMITGLYSASADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPMVFQQAFHVMRSGRPGPVLI
DLPIDVQLAEIEFDEETYASLPVYKPAATQAQIDKAMEMIVGAKRPLIVSGGGVINADASDLLVEFAELTGIPVIPTLMG
WGTIPDDHPLMAGMCGLQTSHRYGNANMLASDFVVGIGNRWANRHTGSVEVYTEGRKFIHVDIEPTQIGRVFGPDFGIVS
DAKAALELFVVAAKALKAAGKLPDWSTWANECLERKQTMLRKSHFDAVPLKPQRVYEEMNEALGRDVTYVTTIGLSQIAG
AQFLHVYNPRHWINCGQAGPLGWTLPAALGVRAARPDAKIVALSGDYDFQFMVEELAVGAQHKLPYLHVVVNNSYLGLIR
QAQRGFNMDFEVSLAFDNINSEEAGAYGVDHVAVAEGLGCKAIRVRTPNEFKDAFANAQKLMDEHQVPVVVEFILERVTN
IAMGLEINAVNEFEEILDVPLDQVRALEPAE

Sequences:

>Translated_591_residues
MARMRAVDAAVRVLEKEGIHCAFGVPGAAINPLYSAMKARGSIRHVLARHVEGASHMAEGYTRAVAGNIGVCIGTSGPAG
TDMITGLYSASADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPMVFQQAFHVMRSGRPGPVLI
DLPIDVQLAEIEFDEETYASLPVYKPAATQAQIDKAMEMIVGAKRPLIVSGGGVINADASDLLVEFAELTGIPVIPTLMG
WGTIPDDHPLMAGMCGLQTSHRYGNANMLASDFVVGIGNRWANRHTGSVEVYTEGRKFIHVDIEPTQIGRVFGPDFGIVS
DAKAALELFVVAAKALKAAGKLPDWSTWANECLERKQTMLRKSHFDAVPLKPQRVYEEMNEALGRDVTYVTTIGLSQIAG
AQFLHVYNPRHWINCGQAGPLGWTLPAALGVRAARPDAKIVALSGDYDFQFMVEELAVGAQHKLPYLHVVVNNSYLGLIR
QAQRGFNMDFEVSLAFDNINSEEAGAYGVDHVAVAEGLGCKAIRVRTPNEFKDAFANAQKLMDEHQVPVVVEFILERVTN
IAMGLEINAVNEFEEILDVPLDQVRALEPAE
>Mature_590_residues
ARMRAVDAAVRVLEKEGIHCAFGVPGAAINPLYSAMKARGSIRHVLARHVEGASHMAEGYTRAVAGNIGVCIGTSGPAGT
DMITGLYSASADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPMVFQQAFHVMRSGRPGPVLID
LPIDVQLAEIEFDEETYASLPVYKPAATQAQIDKAMEMIVGAKRPLIVSGGGVINADASDLLVEFAELTGIPVIPTLMGW
GTIPDDHPLMAGMCGLQTSHRYGNANMLASDFVVGIGNRWANRHTGSVEVYTEGRKFIHVDIEPTQIGRVFGPDFGIVSD
AKAALELFVVAAKALKAAGKLPDWSTWANECLERKQTMLRKSHFDAVPLKPQRVYEEMNEALGRDVTYVTTIGLSQIAGA
QFLHVYNPRHWINCGQAGPLGWTLPAALGVRAARPDAKIVALSGDYDFQFMVEELAVGAQHKLPYLHVVVNNSYLGLIRQ
AQRGFNMDFEVSLAFDNINSEEAGAYGVDHVAVAEGLGCKAIRVRTPNEFKDAFANAQKLMDEHQVPVVVEFILERVTNI
AMGLEINAVNEFEEILDVPLDQVRALEPAE

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=593, Percent_Identity=25.1264755480607, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=24.1563055062167, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1786717, Length=582, Percent_Identity=74.0549828178694, Blast_Score=905, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=542, Percent_Identity=32.4723247232472, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1790104, Length=484, Percent_Identity=34.504132231405, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=24.8638838475499, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788716, Length=473, Percent_Identity=27.061310782241, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=551, Percent_Identity=24.5009074410163, Blast_Score=112, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=551, Percent_Identity=24.5009074410163, Blast_Score=112, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=546, Percent_Identity=30.952380952381, Blast_Score=253, Evalue=9e-68,
Organism=Saccharomyces cerevisiae, GI6320816, Length=562, Percent_Identity=24.9110320284698, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321524, Length=487, Percent_Identity=21.9712525667351, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19922626, Length=515, Percent_Identity=26.6019417475728, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64160; Mature: 64029

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMRAVDAAVRVLEKEGIHCAFGVPGAAINPLYSAMKARGSIRHVLARHVEGASHMAEG
CCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YTRAVAGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITGQAPRARLYKEDFQAVDIE
HHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPMVFQQAFHVMRSGRPGPVLIDLPIDVQLAEIEFDEETYAS
HHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCHHHCC
LPVYKPAATQAQIDKAMEMIVGAKRPLIVSGGGVINADASDLLVEFAELTGIPVIPTLMG
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCEEECCHHHHHHHHHHHCCCCCHHHHHC
WGTIPDDHPLMAGMCGLQTSHRYGNANMLASDFVVGIGNRWANRHTGSVEVYTEGRKFIH
CCCCCCCCCHHHHHHCCCCCCCCCCCCEEHHHHEEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDFGIVSDAKAALELFVVAAKALKAAGKLPDWSTWANECLERKQTML
EECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKSHFDAVPLKPQRVYEEMNEALGRDVTYVTTIGLSQIAGAQFLHVYNPRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCEEEEECCCCEECCCCCCC
LGWTLPAALGVRAARPDAKIVALSGDYDFQFMVEELAVGAQHKLPYLHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHH
QAQRGFNMDFEVSLAFDNINSEEAGAYGVDHVAVAEGLGCKAIRVRTPNEFKDAFANAQK
HHHHCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
LMDEHQVPVVVEFILERVTNIAMGLEINAVNEFEEILDVPLDQVRALEPAE
HHHHCCCHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHCCHHHHCCCCCCC
>Mature Secondary Structure 
ARMRAVDAAVRVLEKEGIHCAFGVPGAAINPLYSAMKARGSIRHVLARHVEGASHMAEG
CCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YTRAVAGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITGQAPRARLYKEDFQAVDIE
HHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPMVFQQAFHVMRSGRPGPVLIDLPIDVQLAEIEFDEETYAS
HHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCHHHCC
LPVYKPAATQAQIDKAMEMIVGAKRPLIVSGGGVINADASDLLVEFAELTGIPVIPTLMG
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCEEECCHHHHHHHHHHHCCCCCHHHHHC
WGTIPDDHPLMAGMCGLQTSHRYGNANMLASDFVVGIGNRWANRHTGSVEVYTEGRKFIH
CCCCCCCCCHHHHHHCCCCCCCCCCCCEEHHHHEEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDFGIVSDAKAALELFVVAAKALKAAGKLPDWSTWANECLERKQTML
EECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKSHFDAVPLKPQRVYEEMNEALGRDVTYVTTIGLSQIAGAQFLHVYNPRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCEEEEECCCCEECCCCCCC
LGWTLPAALGVRAARPDAKIVALSGDYDFQFMVEELAVGAQHKLPYLHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHH
QAQRGFNMDFEVSLAFDNINSEEAGAYGVDHVAVAEGLGCKAIRVRTPNEFKDAFANAQK
HHHHCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
LMDEHQVPVVVEFILERVTNIAMGLEINAVNEFEEILDVPLDQVRALEPAE
HHHHCCCHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]