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The map label for this gene is purN [H]

Identifier: 154246266

GI number: 154246266

Start: 2597123

End: 2597791

Strand: Direct

Name: purN [H]

Synonym: Xaut_2324

Alternate gene names: 154246266

Gene position: 2597123-2597791 (Clockwise)

Preceding gene: 154246265

Following gene: 154246272

Centisome position: 48.92

GC content: 68.01

Gene sequence:

>669_bases
GTGAGCGCTGGGTCGAGTGAGGCGCCGCGCCGCACCGCCGTCCTCATTTCGGGCCGGGGCTCCAATATGGCGGCGCTGGT
GCGCGCGGCCGAACAGGAAGACTTTCCGGCCGAGATCGCCCTGGTGCTCTCCAACCGCGCCGATGCGGCTGGGCTGGATT
TCGCCAAGGATCATGGCATCCCCACTCTGGTGCTCTCCCACAAGGGCTATTCCGATCGCCTCGCCTTCGACGCGGCGCTG
GATGCGCACCTGAAGGCGGAGGGCATTGAGATCGTCTGCCTCGCCGGCTTCATGCGCCTGCTCACCCCCTGGCTGGTGGA
GCGCTGGCGCAACCGCATGATTAACGTGCATCCCTCGCTGCTGCCGAGCTTCAAGGGCCTGCACACCCACGAACGGGCGC
TTGAGGCCGGGGTGCGGGTACATGGCTGCACCGTGCATTTCGTGCGGGCGGAGATGGACGAGGGGCCGATCATCCTGCAG
GCCGTGGTGCCCATCGAGCCCGGCGACACGCCGGACGTGCTCGCCGACCGAGTGCTGGAACAAGAGCACATCATCTATCC
CAAGGGCCTGGAACTCCTCGCCGCCGGCCGGTTGACGGTCGAAGACGAGCGGGTGGCGATCGCGGGCGCCAAGGGCGGGC
TGAAACCGCTGGTGGTGCCGGCGGTGTGA

Upstream 100 bases:

>100_bases
CCGATGCCGGCGAGTGCGTCATCCGCCTCGGCGTCATCGAGCCCGGTGAGGGGCCGGCCCATGTGGCCTATGAAGGCACT
ACTGATTTCGGATTCGCGTC

Downstream 100 bases:

>100_bases
GACGCCCCCTCTCCGCGAGCGGAGAGGGTCTGTCCAATGTCAATCCGCCAGCTTCTCCAGCCGCACGCGGTTGTCGTGGA
ATGCGGCGCCGCCCACCGGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSAGSSEAPRRTAVLISGRGSNMAALVRAAEQEDFPAEIALVLSNRADAAGLDFAKDHGIPTLVLSHKGYSDRLAFDAAL
DAHLKAEGIEIVCLAGFMRLLTPWLVERWRNRMINVHPSLLPSFKGLHTHERALEAGVRVHGCTVHFVRAEMDEGPIILQ
AVVPIEPGDTPDVLADRVLEQEHIIYPKGLELLAAGRLTVEDERVAIAGAKGGLKPLVVPAV

Sequences:

>Translated_222_residues
MSAGSSEAPRRTAVLISGRGSNMAALVRAAEQEDFPAEIALVLSNRADAAGLDFAKDHGIPTLVLSHKGYSDRLAFDAAL
DAHLKAEGIEIVCLAGFMRLLTPWLVERWRNRMINVHPSLLPSFKGLHTHERALEAGVRVHGCTVHFVRAEMDEGPIILQ
AVVPIEPGDTPDVLADRVLEQEHIIYPKGLELLAAGRLTVEDERVAIAGAKGGLKPLVVPAV
>Mature_221_residues
SAGSSEAPRRTAVLISGRGSNMAALVRAAEQEDFPAEIALVLSNRADAAGLDFAKDHGIPTLVLSHKGYSDRLAFDAALD
AHLKAEGIEIVCLAGFMRLLTPWLVERWRNRMINVHPSLLPSFKGLHTHERALEAGVRVHGCTVHFVRAEMDEGPIILQA
VVPIEPGDTPDVLADRVLEQEHIIYPKGLELLAAGRLTVEDERVAIAGAKGGLKPLVVPAV

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=52.1052631578947, Blast_Score=205, Evalue=2e-53,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=52.1052631578947, Blast_Score=205, Evalue=2e-53,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=52.1052631578947, Blast_Score=205, Evalue=2e-53,
Organism=Escherichia coli, GI1788846, Length=198, Percent_Identity=43.4343434343434, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1787483, Length=198, Percent_Identity=31.3131313131313, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=185, Percent_Identity=43.2432432432432, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320616, Length=200, Percent_Identity=31.5, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582400, Length=200, Percent_Identity=46, Blast_Score=185, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23906; Mature: 23775

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGSSEAPRRTAVLISGRGSNMAALVRAAEQEDFPAEIALVLSNRADAAGLDFAKDHGI
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHCCC
PTLVLSHKGYSDRLAFDAALDAHLKAEGIEIVCLAGFMRLLTPWLVERWRNRMINVHPSL
CEEEEECCCCCCCEEHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCEEEECHHH
LPSFKGLHTHERALEAGVRVHGCTVHFVRAEMDEGPIILQAVVPIEPGDTPDVLADRVLE
CCCCCCCCHHHHHHHCCCEEEEEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHH
QEHIIYPKGLELLAAGRLTVEDERVAIAGAKGGLKPLVVPAV
HCCEECCCCCEEEECCCEEEECCEEEEEECCCCCCCEEECCC
>Mature Secondary Structure 
SAGSSEAPRRTAVLISGRGSNMAALVRAAEQEDFPAEIALVLSNRADAAGLDFAKDHGI
CCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHCCC
PTLVLSHKGYSDRLAFDAALDAHLKAEGIEIVCLAGFMRLLTPWLVERWRNRMINVHPSL
CEEEEECCCCCCCEEHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCEEEECHHH
LPSFKGLHTHERALEAGVRVHGCTVHFVRAEMDEGPIILQAVVPIEPGDTPDVLADRVLE
CCCCCCCCHHHHHHHCCCEEEEEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHH
QEHIIYPKGLELLAAGRLTVEDERVAIAGAKGGLKPLVVPAV
HCCEECCCCCEEEECCCEEEECCEEEEEECCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]