Click here to switch to the map view.

The map label for this gene is hupR1 [H]

Identifier: 154246127

GI number: 154246127

Start: 2443307

End: 2444800

Strand: Direct

Name: hupR1 [H]

Synonym: Xaut_2184

Alternate gene names: 154246127

Gene position: 2443307-2444800 (Clockwise)

Preceding gene: 154246126

Following gene: 154246128

Centisome position: 46.02

GC content: 68.47

Gene sequence:

>1494_bases
GTGAACCGCCGCCCCGCCATCCTCGTGGTGGACGACGAGCCGCGCTCGGTGGAAGTCATTGCCCGCGTGCTCGACGAGGA
GTTCGACGTCTTCACCGCGCTCAACGCGGACGAGGCCCGGCGCATCCTCGAGAACGAGTGGATCCAGGTGATCTTCTGCG
ACCAGCGCATGCCGCACACCTCCGGCGTCGCGCTGCTCACCGAGGTGCGCGAGCGCTGGCCGGATATCCTGCGCATCATC
GTCACCGGCTATACCGAGCCGCAGGACATGATCGGCGCCATCAACGAGGCCGGCATCTACCAGTTCATCCCCAAGCCGTG
GCACCCGGACCAATTGCTGCTGGCCGCGCGCAACGCCGCCCACCTGTTCCAGCTCCAGCGCGACCATGAGCGGCTGAACC
TGGAAATGAAGCTCACCGCCGTCACCGCCGAGGCGCGGCTCGGCGCCCAGCAGGCGCGGGTGAAGCAGAATTTCCATTTC
GACACCCTCATCCGCGCCCCTGGCAGCGCGCTGAGTGCGGTGTGCCTGCGGGCGGCGCAGGTGGCGACTTTCGACGTGCC
GGTGCTCGTCCATGGCGAGACCGGCACCGGCAAGGAGCTGCTCGCCCGCGCCATCCATTATTCGAGCCTTCGCTCCGACC
GGCCGTTCTTCGCCGTCAATTGCGGGGCGATCCCGGACGAGCTTCTGGAATCGGAGCTGTTCGGCCACAAGAAGGGGTCG
TTCACCGGCGCCCACGTCACCCGCATCGGCCTGCTCGACCAGGCGGACGGCGGCACCATCTTCCTCGACGAGATCGGTGA
TGTGTCCCCCGCCTTCCAGGTGAAGCTGCTGCGCTTCCTGCAGGAGGGCGAGATCCGCCCGGTGGGCTCCAACGAATCCA
AGCGGGTCAACGTGCGTGTGCTCGCCGCCACCCATCGCGATCTCTATGGCGAGGTAGGGCGAGGCCGCTTCCGCGAGGAT
CTCTATTACCGCCTGTGCGCCATGGTGCTGACCCTGCCGGCCCTGCGCGAACGGCGCCCCGACATCCCGGTGCTGGCCCA
GAGCATCCTCGATGCGCTTTCCGCCCACCACGGCAAGCGCGTCAAGGGCTTCACCGAGGAAGCGCTCGCCTGCATGCAGG
CCTATGACTGGCCCGGCAACGTGCGCGAGCTGCAGAACGAGATCACGCGCATGCTGGTGCTGGCCGAGCGCGACACGCTC
TCCGCCGACCTGCTGTCGCCCCACGTGGTGCGCGCCGCCGCCGCCGAGACACGGGCGGTGGAGACCCGCGCATCCGAGGC
GGGGGCGTCTCAGGCCATCTCCCTGCCCGACGGCGGGCCGCTGAAGGAGCGGGTGGAGCGCATGGAAGCCACCATCCTCA
CCGAGACGCTGGTGCGCTGCCGCTGGAACAAGAGCCGCGCGGCGGAGGAGCTTGGCCTCTCCCGCGTCGGACTTCGGGCG
AAACTCGACCGCTACGGGATCTCCCGCGGCGGGGCCTTTTCCAAGTCGCATTGA

Upstream 100 bases:

>100_bases
GGCTGGAGAAGGCCCGCGCGGAGCGCCTCGACGCGCTCGCCGCCGCCCGCGAGCGCGAGGCGCAGGCCCTGCGCGCGGCC
ATGGTGGCGGGAGGCGCGCG

Downstream 100 bases:

>100_bases
GGGGGTCGGGATGACCGCCTTTTATCGGCAAATGCTGTCAAGCCCGGCCATGACGGTTGCGAAAGCGGCGTGGTTCCACC
GAATTTTGACCCTTTAGGAG

Product: two component sigma54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNRRPAILVVDDEPRSVEVIARVLDEEFDVFTALNADEARRILENEWIQVIFCDQRMPHTSGVALLTEVRERWPDILRII
VTGYTEPQDMIGAINEAGIYQFIPKPWHPDQLLLAARNAAHLFQLQRDHERLNLEMKLTAVTAEARLGAQQARVKQNFHF
DTLIRAPGSALSAVCLRAAQVATFDVPVLVHGETGTGKELLARAIHYSSLRSDRPFFAVNCGAIPDELLESELFGHKKGS
FTGAHVTRIGLLDQADGGTIFLDEIGDVSPAFQVKLLRFLQEGEIRPVGSNESKRVNVRVLAATHRDLYGEVGRGRFRED
LYYRLCAMVLTLPALRERRPDIPVLAQSILDALSAHHGKRVKGFTEEALACMQAYDWPGNVRELQNEITRMLVLAERDTL
SADLLSPHVVRAAAAETRAVETRASEAGASQAISLPDGGPLKERVERMEATILTETLVRCRWNKSRAAEELGLSRVGLRA
KLDRYGISRGGAFSKSH

Sequences:

>Translated_497_residues
MNRRPAILVVDDEPRSVEVIARVLDEEFDVFTALNADEARRILENEWIQVIFCDQRMPHTSGVALLTEVRERWPDILRII
VTGYTEPQDMIGAINEAGIYQFIPKPWHPDQLLLAARNAAHLFQLQRDHERLNLEMKLTAVTAEARLGAQQARVKQNFHF
DTLIRAPGSALSAVCLRAAQVATFDVPVLVHGETGTGKELLARAIHYSSLRSDRPFFAVNCGAIPDELLESELFGHKKGS
FTGAHVTRIGLLDQADGGTIFLDEIGDVSPAFQVKLLRFLQEGEIRPVGSNESKRVNVRVLAATHRDLYGEVGRGRFRED
LYYRLCAMVLTLPALRERRPDIPVLAQSILDALSAHHGKRVKGFTEEALACMQAYDWPGNVRELQNEITRMLVLAERDTL
SADLLSPHVVRAAAAETRAVETRASEAGASQAISLPDGGPLKERVERMEATILTETLVRCRWNKSRAAEELGLSRVGLRA
KLDRYGISRGGAFSKSH
>Mature_497_residues
MNRRPAILVVDDEPRSVEVIARVLDEEFDVFTALNADEARRILENEWIQVIFCDQRMPHTSGVALLTEVRERWPDILRII
VTGYTEPQDMIGAINEAGIYQFIPKPWHPDQLLLAARNAAHLFQLQRDHERLNLEMKLTAVTAEARLGAQQARVKQNFHF
DTLIRAPGSALSAVCLRAAQVATFDVPVLVHGETGTGKELLARAIHYSSLRSDRPFFAVNCGAIPDELLESELFGHKKGS
FTGAHVTRIGLLDQADGGTIFLDEIGDVSPAFQVKLLRFLQEGEIRPVGSNESKRVNVRVLAATHRDLYGEVGRGRFRED
LYYRLCAMVLTLPALRERRPDIPVLAQSILDALSAHHGKRVKGFTEEALACMQAYDWPGNVRELQNEITRMLVLAERDTL
SADLLSPHVVRAAAAETRAVETRASEAGASQAISLPDGGPLKERVERMEATILTETLVRCRWNKSRAAEELGLSRVGLRA
KLDRYGISRGGAFSKSH

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=479, Percent_Identity=39.0396659707724, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1788550, Length=483, Percent_Identity=35.8178053830228, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1788905, Length=407, Percent_Identity=39.3120393120393, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1790299, Length=491, Percent_Identity=34.2158859470468, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI87082117, Length=310, Percent_Identity=43.2258064516129, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789233, Length=309, Percent_Identity=39.8058252427184, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1789087, Length=322, Percent_Identity=39.7515527950311, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI87082152, Length=223, Percent_Identity=51.1210762331839, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1787583, Length=367, Percent_Identity=35.6948228882834, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI87081872, Length=335, Percent_Identity=38.8059701492537, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1786524, Length=217, Percent_Identity=47.0046082949309, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1789828, Length=326, Percent_Identity=33.7423312883436, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87081858, Length=311, Percent_Identity=32.1543408360129, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55368; Mature: 55368

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRPAILVVDDEPRSVEVIARVLDEEFDVFTALNADEARRILENEWIQVIFCDQRMPHT
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCCEEEEEECCCCCCC
SGVALLTEVRERWPDILRIIVTGYTEPQDMIGAINEAGIYQFIPKPWHPDQLLLAARNAA
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHH
HLFQLQRDHERLNLEMKLTAVTAEARLGAQQARVKQNFHFDTLIRAPGSALSAVCLRAAQ
HHHHHHHHHHHCCEEEEEEEEEHHHHHCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHH
VATFDVPVLVHGETGTGKELLARAIHYSSLRSDRPFFAVNCGAIPDELLESELFGHKKGS
HEEECCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCC
FTGAHVTRIGLLDQADGGTIFLDEIGDVSPAFQVKLLRFLQEGEIRPVGSNESKRVNVRV
CCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
LAATHRDLYGEVGRGRFREDLYYRLCAMVLTLPALRERRPDIPVLAQSILDALSAHHGKR
EEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
VKGFTEEALACMQAYDWPGNVRELQNEITRMLVLAERDTLSADLLSPHVVRAAAAETRAV
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHH
ETRASEAGASQAISLPDGGPLKERVERMEATILTETLVRCRWNKSRAAEELGLSRVGLRA
HHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHH
KLDRYGISRGGAFSKSH
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNRRPAILVVDDEPRSVEVIARVLDEEFDVFTALNADEARRILENEWIQVIFCDQRMPHT
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCCEEEEEECCCCCCC
SGVALLTEVRERWPDILRIIVTGYTEPQDMIGAINEAGIYQFIPKPWHPDQLLLAARNAA
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHH
HLFQLQRDHERLNLEMKLTAVTAEARLGAQQARVKQNFHFDTLIRAPGSALSAVCLRAAQ
HHHHHHHHHHHCCEEEEEEEEEHHHHHCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHH
VATFDVPVLVHGETGTGKELLARAIHYSSLRSDRPFFAVNCGAIPDELLESELFGHKKGS
HEEECCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCC
FTGAHVTRIGLLDQADGGTIFLDEIGDVSPAFQVKLLRFLQEGEIRPVGSNESKRVNVRV
CCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
LAATHRDLYGEVGRGRFREDLYYRLCAMVLTLPALRERRPDIPVLAQSILDALSAHHGKR
EEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
VKGFTEEALACMQAYDWPGNVRELQNEITRMLVLAERDTLSADLLSPHVVRAAAAETRAV
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHH
ETRASEAGASQAISLPDGGPLKERVERMEATILTETLVRCRWNKSRAAEELGLSRVGLRA
HHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHH
KLDRYGISRGGAFSKSH
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]