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The map label for this gene is cysG [H]

Identifier: 154245026

GI number: 154245026

Start: 1236380

End: 1237852

Strand: Reverse

Name: cysG [H]

Synonym: Xaut_1077

Alternate gene names: 154245026

Gene position: 1237852-1236380 (Counterclockwise)

Preceding gene: 154245027

Following gene: 154245023

Centisome position: 23.32

GC content: 69.38

Gene sequence:

>1473_bases
ATGCGAAACATCTCCAGGCAGCCGAGTGACCAGGACGCGGCGCGCATCCGCCCGCTCACCGTGCTGCCCGTGTTCCTCGA
TCTGGCCGGCAAGCGCGCCGTCGTCGCCGGCGGCTCGGCAGCCGCGTGCTGGAAGGCCGAGCTGCTGCTGTCCGCCGGAG
CCAAGGTCTTTGTCCATGCGTCCGACGAAGACCTGTGCGAGGATTTCGCCAAACTGATCGAGCGGCATGGCCCGGCCGCC
CTTCACCACGGCAGATCGTGGGACAGGACCGATCTCGCCGGCGCGGCCGTCGCCATCTGCGACGCGCGGGACGAACAGGA
GGCAGCGGCGTTCGCGGCAGCCGCAGCCGCAGCCGGCGTGCCCTGCAACGTGATCGACCAGTCGGCCTTCTGCAGCTTCC
AGTTCGGCGCGATCGTCAACCGCTCTCCCGTGGTGATCGGTATTTCCACTACCGGAGCAGCGCCCATTCTGGGGCAGGCC
ATCCGCCGGCGCATCGAGACGCTCTTGATGCCGCCGCTGGCGGAATGGGCCGCGCTGGCGGCCCGCATCCGAGGCCGGGT
GGCCGCGACCCTGAACCCGGGCGCGGAGCGGCGGCGCTTCTGGGAGCGGCTTGCGGAACGTGCTTTCGGCGACGCCCCGG
GGCCGCAGGTGGAACAGGAGCTGTTCGAGGAGGCGCGAACGGTGGGCGCATCGCGTGCGAGCGCGGGCAGGGTCACGCTG
GTGGGCGCCGGCCCGGGCGAGGCTGAGCTGTTGACCTTGAAGGCGGTTCGCGCGCTTCAGTCTGCCGATGTGATCCTGTT
TGATGATCTGGTTTCCGACGAGGTGCTTGAGCTGGCCCGCCGCGAGGCGAAGCGCATCCAGGTGGGCAAGCGCGGCGGAC
GGCCGAGCTGCAGGCAGGAGGACATCAATGCCCTCATGCTGACGCTCGCCAAGGCCGGCCGCCATGTGGTGCGGCTCAAA
TCCGGAGATCCGATGATCTTCGGCCGCGCCGGCGAAGAGATTGCCATGCTGGAAGCGGCCGGCATTCCCGTCGCAATCGT
CCCCGGCATTTCCGCTGGGGTGGCGCTCGCGTCGGCCCTGCGCATGTCTCTGACCCATCGCGACCACGCGAAATCGGTCC
GCTTCGTGACCGGCCATTCCCGCAACGGCGGCCTTCCCGAGGATCTCGACTGGCGGTCGATTGCCGATCCCTCGGCCACG
ACCATTTTCTACATGGGCGGCCGCAACGCCGGGCAGATCGCAGACCGCCTCATCAGCCTCGGCGTGCCTGCATCCATGCC
GGTCGCGGTCGGTGCGGGCCTTGGGCGTCGCGAGCAGCAATTGCGGATCGCGGACCTTGCGACGCTCGGCGAGGCCGTCA
ACCAGATGAACAGCGACCAGCCGATCGTCATCGGCGTTGGCCGGGCCTTCGCGCAGCACCGCGCGACCGGCCTCGAAAGG
GATCCGCCACTGCCGGTTGCCGCGACGGGCTGA

Upstream 100 bases:

>100_bases
GCCATTGCCAGCGGGTGTCGCACGGTGAAGGCCGTCGGCGATGCAACGCAGGCCGGTACCAACTGCGGGTCATGTCGGGC
CGAGATCAAGGGGATCATCG

Downstream 100 bases:

>100_bases
GACGGCGCCGAAACCTCGCCAGCATTACAGATGCCATCGCCCGATCGCGGGCTGAGCACCAGACAGGTCGCGGCCTTCAG
CCTGATGCGGCCGGGCACAA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MRNISRQPSDQDAARIRPLTVLPVFLDLAGKRAVVAGGSAAACWKAELLLSAGAKVFVHASDEDLCEDFAKLIERHGPAA
LHHGRSWDRTDLAGAAVAICDARDEQEAAAFAAAAAAAGVPCNVIDQSAFCSFQFGAIVNRSPVVIGISTTGAAPILGQA
IRRRIETLLMPPLAEWAALAARIRGRVAATLNPGAERRRFWERLAERAFGDAPGPQVEQELFEEARTVGASRASAGRVTL
VGAGPGEAELLTLKAVRALQSADVILFDDLVSDEVLELARREAKRIQVGKRGGRPSCRQEDINALMLTLAKAGRHVVRLK
SGDPMIFGRAGEEIAMLEAAGIPVAIVPGISAGVALASALRMSLTHRDHAKSVRFVTGHSRNGGLPEDLDWRSIADPSAT
TIFYMGGRNAGQIADRLISLGVPASMPVAVGAGLGRREQQLRIADLATLGEAVNQMNSDQPIVIGVGRAFAQHRATGLER
DPPLPVAATG

Sequences:

>Translated_490_residues
MRNISRQPSDQDAARIRPLTVLPVFLDLAGKRAVVAGGSAAACWKAELLLSAGAKVFVHASDEDLCEDFAKLIERHGPAA
LHHGRSWDRTDLAGAAVAICDARDEQEAAAFAAAAAAAGVPCNVIDQSAFCSFQFGAIVNRSPVVIGISTTGAAPILGQA
IRRRIETLLMPPLAEWAALAARIRGRVAATLNPGAERRRFWERLAERAFGDAPGPQVEQELFEEARTVGASRASAGRVTL
VGAGPGEAELLTLKAVRALQSADVILFDDLVSDEVLELARREAKRIQVGKRGGRPSCRQEDINALMLTLAKAGRHVVRLK
SGDPMIFGRAGEEIAMLEAAGIPVAIVPGISAGVALASALRMSLTHRDHAKSVRFVTGHSRNGGLPEDLDWRSIADPSAT
TIFYMGGRNAGQIADRLISLGVPASMPVAVGAGLGRREQQLRIADLATLGEAVNQMNSDQPIVIGVGRAFAQHRATGLER
DPPLPVAATG
>Mature_490_residues
MRNISRQPSDQDAARIRPLTVLPVFLDLAGKRAVVAGGSAAACWKAELLLSAGAKVFVHASDEDLCEDFAKLIERHGPAA
LHHGRSWDRTDLAGAAVAICDARDEQEAAAFAAAAAAAGVPCNVIDQSAFCSFQFGAIVNRSPVVIGISTTGAAPILGQA
IRRRIETLLMPPLAEWAALAARIRGRVAATLNPGAERRRFWERLAERAFGDAPGPQVEQELFEEARTVGASRASAGRVTL
VGAGPGEAELLTLKAVRALQSADVILFDDLVSDEVLELARREAKRIQVGKRGGRPSCRQEDINALMLTLAKAGRHVVRLK
SGDPMIFGRAGEEIAMLEAAGIPVAIVPGISAGVALASALRMSLTHRDHAKSVRFVTGHSRNGGLPEDLDWRSIADPSAT
TIFYMGGRNAGQIADRLISLGVPASMPVAVGAGLGRREQQLRIADLATLGEAVNQMNSDQPIVIGVGRAFAQHRATGLER
DPPLPVAATG

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=450, Percent_Identity=38.8888888888889, Blast_Score=291, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6322922, Length=234, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 51696; Mature: 51696

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNISRQPSDQDAARIRPLTVLPVFLDLAGKRAVVAGGSAAACWKAELLLSAGAKVFVHA
CCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEC
SDEDLCEDFAKLIERHGPAALHHGRSWDRTDLAGAAVAICDARDEQEAAAFAAAAAAAGV
CCHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHCCC
PCNVIDQSAFCSFQFGAIVNRSPVVIGISTTGAAPILGQAIRRRIETLLMPPLAEWAALA
CCCCCCCCCCCEEEECCEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
ARIRGRVAATLNPGAERRRFWERLAERAFGDAPGPQVEQELFEEARTVGASRASAGRVTL
HHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEE
VGAGPGEAELLTLKAVRALQSADVILFDDLVSDEVLELARREAKRIQVGKRGGRPSCRQE
EECCCCCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
DINALMLTLAKAGRHVVRLKSGDPMIFGRAGEEIAMLEAAGIPVAIVPGISAGVALASAL
HHHHHHHHHHHCCCEEEEEECCCCEEEECCCCEEEEEEECCCCEEEECCCHHHHHHHHHH
RMSLTHRDHAKSVRFVTGHSRNGGLPEDLDWRSIADPSATTIFYMGGRNAGQIADRLISL
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHC
GVPASMPVAVGAGLGRREQQLRIADLATLGEAVNQMNSDQPIVIGVGRAFAQHRATGLER
CCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCC
DPPLPVAATG
CCCCCCCCCC
>Mature Secondary Structure
MRNISRQPSDQDAARIRPLTVLPVFLDLAGKRAVVAGGSAAACWKAELLLSAGAKVFVHA
CCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEC
SDEDLCEDFAKLIERHGPAALHHGRSWDRTDLAGAAVAICDARDEQEAAAFAAAAAAAGV
CCHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHCCC
PCNVIDQSAFCSFQFGAIVNRSPVVIGISTTGAAPILGQAIRRRIETLLMPPLAEWAALA
CCCCCCCCCCCEEEECCEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
ARIRGRVAATLNPGAERRRFWERLAERAFGDAPGPQVEQELFEEARTVGASRASAGRVTL
HHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEE
VGAGPGEAELLTLKAVRALQSADVILFDDLVSDEVLELARREAKRIQVGKRGGRPSCRQE
EECCCCCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
DINALMLTLAKAGRHVVRLKSGDPMIFGRAGEEIAMLEAAGIPVAIVPGISAGVALASAL
HHHHHHHHHHHCCCEEEEEECCCCEEEECCCCEEEEEEECCCCEEEECCCHHHHHHHHHH
RMSLTHRDHAKSVRFVTGHSRNGGLPEDLDWRSIADPSATTIFYMGGRNAGQIADRLISL
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHC
GVPASMPVAVGAGLGRREQQLRIADLATLGEAVNQMNSDQPIVIGVGRAFAQHRATGLER
CCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCC
DPPLPVAATG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA