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The map label for this gene is nasA [H]

Identifier: 154245027

GI number: 154245027

Start: 1237824

End: 1240517

Strand: Reverse

Name: nasA [H]

Synonym: Xaut_1078

Alternate gene names: 154245027

Gene position: 1240517-1237824 (Counterclockwise)

Preceding gene: 154245028

Following gene: 154245026

Centisome position: 23.37

GC content: 67.56

Gene sequence:

>2694_bases
ATGGCGACGGGTGCTGCTCACGACGTGAAGACCACCTGCCCGTATTGCGGCGTCGGCTGCGGCGTGATCGCCACGGTCAC
ACCGGACGGGTCGGTTTCGGTGAGCGGCGACCCCGAGCATCCGGCCAATTTCGGCCGGCTCTGCTCGAAAGGCTCGGCGC
TGGCCGAGACCATCGATCTGGACGGGCGCCTTCTGCGCCCGCACATCGACGGCCGGCCGGCAGACTGGGACGAGGCCCTC
GATCTGGTGGCCGCGCGGTTTGCATCCACCATCGCCGAACACGGCCCGGACTCGGTGGCCTTCTACGTCTCCGGCCAGCT
TCTGACCGAGGATTATTACGTCGCCAACAAGCTGATGAAGGGGTTCATCGGCTCCGCCAACATCGACACCAATTCGCGGC
TTTGCATGGCCTCGTCCGTGGCCGGCCACCGCCGCGCCTTCGGCACCGACACCGTGCCCGGCAACTATGAAGACCTCGAG
CTCGCAGACCTGATCGTGCTGGTGGGCTCGAACCTCGCCTGGTGCCATCCGGTGCTCTACCAGCGCATCGCCGCGGAGAA
AGAGCGGCGCCCCTCGCTCAAGATCGTCGTGATCGACCCGCGCCGCACCATGACGGCGGACATCGCCGACATGCATCTGG
CGATCTCGCCCGACGGCGACGTGGCGTTGTTCGGCGGCCTTCTGAGCTGGATCGCGAACACCGGTGCGCTCAACTATTCC
TATATTTCGAATTTCACGACAGGGGCGGCACAGGCGCTGCGCTTCGGCGCGCAAGACCTCCGGGCGGTCAGTCGAAAGTG
CGGCATCGCCCCCTCTCAGCTTATTGAATTTTATTCCCTTTTCACCCGCACCGAGCGCACCGTCACGGTCTACAGCCAGG
GCGTCAACCAGTCGGAAACCGGAACCGACAAGGTCAATGCGATCATCAATTGCCATCTGGCCACCGGCCGGATCGGCAAG
CCGGGCGCCTCGCCCTTTTCCGTCACCGGCCAGCCCAACGCGATGGGCGGTCGCGAGGTCGGAGGCATGGCCAACATGCT
CGCCGCCCATATGGATATCGAGAACGAAACCCATCGCGCGCGGGTTCAACGCTTCTGGCAGGCGCCCACCGTCGCCACGC
GGCCGGGCCGCAAGGCCGTGGAGATGTTCGAAGCCATTGGCCAGGGCCACATCAAGGCCGTCTGGATCGCCGGGACCAAC
CCGGTGGATTCGATGCCCGAGGCCGACCGTGTCAGGGCGGCGCTTGCCGCCTGCCCCTTCGTCGTGGTCTCCGATGTCCT
CGCCGACACCGACACCACGCGCGTAGCCCACGTCCTGCTGCCGGCAGCCGCCTGGGGCGAGAAGGATGGCACGGTCACCA
ATTCGGAGCGGCGGATCTCGCGGCAGCGTCCGTTCCTGCCGCTGCCGGGCTCGGCCCGGCCGGATTGGGACATCATGGCC
GATGTGGCGCGACGCATGGGCTTTGCCGAAGGCTTTTCCTACACCACCGCCGCCGAGGTCTTTGCCGAACACGCCGCCCT
GTCGGCCTTCGAGAATGACGGCGAGCGGGATTTCGACATCGGGGCTCATGCCGGGATCGACGCCGTCGGCTATGAGGCGC
TCGCTCCCTTCCAGTGGCCCCAACGCCGCGGGGCGGTCACCGGTACGGAGCGCTTCTTCGCCGCCGGAGGCTTCTTCACG
CCGGAGGGCAAGGGACGCTTCGTGGCCCCGCGCGAGGCCCGGGCCGGTACCGGCCCGCTGGCAAGGCTGCCGGCGCAGGA
GCCGACCGATCAAGGCTTCATCCTGAACACCGGTCGCGTGCGGGATCACTGGCACACCATGACGCGGACGGGAAAAAGCC
CCCGCCTGTCCCAGCACATGGCAGAGCCGTTCGTGGAAATTCATCCGGACGATGCGGTCCAGAGCGGCATCGCGGAAGCG
AGCCTCGTGCGGCTGTCCAAGGGTCCGGCGCAGGCCATCGCGCGCGCCCTGATCACCAATCGCCAGCGTCGCGGCTCGGT
GTTCGTGCCGATGCACTGGAACGACCGGTTCGCCTCCAACGCCCGCATTGATGCGCTGGTCGATGCCGTCACCGACCCGG
TCTCGGGCCAGCCTGCGCTGAAGCAGGCGCGGGCATCGCTGGCCAGTTTCAATGCCGCGGCCTACGGCTTTGCGGTCGTG
GCCCGCAAGCCGGCGGACATCCCGGCCGACTATTGGGCGATCGCCAGATGCACCGGCGGCTGGCGGCTGGAACTTGCGTT
CGGGCAGGCCAAGGACGACTGGCACGCCTTTGCCCTGCGGCTGTTCGGCGGCGGGGAACATGTGGACATCCTGGCCTACA
ACGACGTTGGAGCGGGCCGCTATCGGATCGCGGCGTTCGACGGCGCTGCGCTTCTGGGTGCGCTCTTCATCGCGCCGCAG
CCGGTCGGCGTGGCCCGTGGCTGGCTGGTGGAGCAGCTTGCGACCAGCCACGACCTCCAGCGGCAGCGGCTGGCGGTCCT
GGCCACCAGGCCCAGCGCCGGCCAATCGGACAAGGGGGCGATCGTGTGCTCCTGCTTCGGGGTGGGAAGCAACGAGATCG
CCGCCGCCATTGCCAGCGGGTGTCGCACGGTGAAGGCCGTCGGCGATGCAACGCAGGCCGGTACCAACTGCGGGTCATGT
CGGGCCGAGATCAAGGGGATCATCGATGCGAAACATCTCCAGGCAGCCGAGTGA

Upstream 100 bases:

>100_bases
ACGGGCGCCGACGAGGGCTCGGTGAAGACCTTCCCGGTGAAGGTCGAGCACGACCAGATCTTCATCGGCCTCGAAGCCGC
CATAGCGAAGGTCGCGTGAC

Downstream 100 bases:

>100_bases
CCAGGACGCGGCGCGCATCCGCCCGCTCACCGTGCTGCCCGTGTTCCTCGATCTGGCCGGCAAGCGCGCCGTCGTCGCCG
GCGGCTCGGCAGCCGCGTGC

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 897; Mature: 896

Protein sequence:

>897_residues
MATGAAHDVKTTCPYCGVGCGVIATVTPDGSVSVSGDPEHPANFGRLCSKGSALAETIDLDGRLLRPHIDGRPADWDEAL
DLVAARFASTIAEHGPDSVAFYVSGQLLTEDYYVANKLMKGFIGSANIDTNSRLCMASSVAGHRRAFGTDTVPGNYEDLE
LADLIVLVGSNLAWCHPVLYQRIAAEKERRPSLKIVVIDPRRTMTADIADMHLAISPDGDVALFGGLLSWIANTGALNYS
YISNFTTGAAQALRFGAQDLRAVSRKCGIAPSQLIEFYSLFTRTERTVTVYSQGVNQSETGTDKVNAIINCHLATGRIGK
PGASPFSVTGQPNAMGGREVGGMANMLAAHMDIENETHRARVQRFWQAPTVATRPGRKAVEMFEAIGQGHIKAVWIAGTN
PVDSMPEADRVRAALAACPFVVVSDVLADTDTTRVAHVLLPAAAWGEKDGTVTNSERRISRQRPFLPLPGSARPDWDIMA
DVARRMGFAEGFSYTTAAEVFAEHAALSAFENDGERDFDIGAHAGIDAVGYEALAPFQWPQRRGAVTGTERFFAAGGFFT
PEGKGRFVAPREARAGTGPLARLPAQEPTDQGFILNTGRVRDHWHTMTRTGKSPRLSQHMAEPFVEIHPDDAVQSGIAEA
SLVRLSKGPAQAIARALITNRQRRGSVFVPMHWNDRFASNARIDALVDAVTDPVSGQPALKQARASLASFNAAAYGFAVV
ARKPADIPADYWAIARCTGGWRLELAFGQAKDDWHAFALRLFGGGEHVDILAYNDVGAGRYRIAAFDGAALLGALFIAPQ
PVGVARGWLVEQLATSHDLQRQRLAVLATRPSAGQSDKGAIVCSCFGVGSNEIAAAIASGCRTVKAVGDATQAGTNCGSC
RAEIKGIIDAKHLQAAE

Sequences:

>Translated_897_residues
MATGAAHDVKTTCPYCGVGCGVIATVTPDGSVSVSGDPEHPANFGRLCSKGSALAETIDLDGRLLRPHIDGRPADWDEAL
DLVAARFASTIAEHGPDSVAFYVSGQLLTEDYYVANKLMKGFIGSANIDTNSRLCMASSVAGHRRAFGTDTVPGNYEDLE
LADLIVLVGSNLAWCHPVLYQRIAAEKERRPSLKIVVIDPRRTMTADIADMHLAISPDGDVALFGGLLSWIANTGALNYS
YISNFTTGAAQALRFGAQDLRAVSRKCGIAPSQLIEFYSLFTRTERTVTVYSQGVNQSETGTDKVNAIINCHLATGRIGK
PGASPFSVTGQPNAMGGREVGGMANMLAAHMDIENETHRARVQRFWQAPTVATRPGRKAVEMFEAIGQGHIKAVWIAGTN
PVDSMPEADRVRAALAACPFVVVSDVLADTDTTRVAHVLLPAAAWGEKDGTVTNSERRISRQRPFLPLPGSARPDWDIMA
DVARRMGFAEGFSYTTAAEVFAEHAALSAFENDGERDFDIGAHAGIDAVGYEALAPFQWPQRRGAVTGTERFFAAGGFFT
PEGKGRFVAPREARAGTGPLARLPAQEPTDQGFILNTGRVRDHWHTMTRTGKSPRLSQHMAEPFVEIHPDDAVQSGIAEA
SLVRLSKGPAQAIARALITNRQRRGSVFVPMHWNDRFASNARIDALVDAVTDPVSGQPALKQARASLASFNAAAYGFAVV
ARKPADIPADYWAIARCTGGWRLELAFGQAKDDWHAFALRLFGGGEHVDILAYNDVGAGRYRIAAFDGAALLGALFIAPQ
PVGVARGWLVEQLATSHDLQRQRLAVLATRPSAGQSDKGAIVCSCFGVGSNEIAAAIASGCRTVKAVGDATQAGTNCGSC
RAEIKGIIDAKHLQAAE
>Mature_896_residues
ATGAAHDVKTTCPYCGVGCGVIATVTPDGSVSVSGDPEHPANFGRLCSKGSALAETIDLDGRLLRPHIDGRPADWDEALD
LVAARFASTIAEHGPDSVAFYVSGQLLTEDYYVANKLMKGFIGSANIDTNSRLCMASSVAGHRRAFGTDTVPGNYEDLEL
ADLIVLVGSNLAWCHPVLYQRIAAEKERRPSLKIVVIDPRRTMTADIADMHLAISPDGDVALFGGLLSWIANTGALNYSY
ISNFTTGAAQALRFGAQDLRAVSRKCGIAPSQLIEFYSLFTRTERTVTVYSQGVNQSETGTDKVNAIINCHLATGRIGKP
GASPFSVTGQPNAMGGREVGGMANMLAAHMDIENETHRARVQRFWQAPTVATRPGRKAVEMFEAIGQGHIKAVWIAGTNP
VDSMPEADRVRAALAACPFVVVSDVLADTDTTRVAHVLLPAAAWGEKDGTVTNSERRISRQRPFLPLPGSARPDWDIMAD
VARRMGFAEGFSYTTAAEVFAEHAALSAFENDGERDFDIGAHAGIDAVGYEALAPFQWPQRRGAVTGTERFFAAGGFFTP
EGKGRFVAPREARAGTGPLARLPAQEPTDQGFILNTGRVRDHWHTMTRTGKSPRLSQHMAEPFVEIHPDDAVQSGIAEAS
LVRLSKGPAQAIARALITNRQRRGSVFVPMHWNDRFASNARIDALVDAVTDPVSGQPALKQARASLASFNAAAYGFAVVA
RKPADIPADYWAIARCTGGWRLELAFGQAKDDWHAFALRLFGGGEHVDILAYNDVGAGRYRIAAFDGAALLGALFIAPQP
VGVARGWLVEQLATSHDLQRQRLAVLATRPSAGQSDKGAIVCSCFGVGSNEIAAAIASGCRTVKAVGDATQAGTNCGSCR
AEIKGIIDAKHLQAAE

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=791, Percent_Identity=28.4450063211125, Blast_Score=298, Evalue=9e-82,
Organism=Escherichia coli, GI3868721, Length=680, Percent_Identity=26.3235294117647, Blast_Score=251, Evalue=2e-67,
Organism=Escherichia coli, GI3868720, Length=267, Percent_Identity=30.7116104868914, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI3868719, Length=269, Percent_Identity=27.1375464684015, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1787778, Length=368, Percent_Identity=25, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 95727; Mature: 95596

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGAAHDVKTTCPYCGVGCGVIATVTPDGSVSVSGDPEHPANFGRLCSKGSALAETIDL
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHCCCCHHHHHHCC
DGRLLRPHIDGRPADWDEALDLVAARFASTIAEHGPDSVAFYVSGQLLTEDYYVANKLMK
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEECHHHHHHHHHHH
GFIGSANIDTNSRLCMASSVAGHRRAFGTDTVPGNYEDLELADLIVLVGSNLAWCHPVLY
HHCCCCCCCCCCCEEEEHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
QRIAAEKERRPSLKIVVIDPRRTMTADIADMHLAISPDGDVALFGGLLSWIANTGALNYS
HHHHHHHHCCCCEEEEEECCCCCCEECCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCHH
YISNFTTGAAQALRFGAQDLRAVSRKCGIAPSQLIEFYSLFTRTERTVTVYSQGVNQSET
HHHCCCHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GTDKVNAIINCHLATGRIGKPGASPFSVTGQPNAMGGREVGGMANMLAAHMDIENETHRA
CCCCEEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCHHCHHHHHHHHHCCCCCHHHHH
RVQRFWQAPTVATRPGRKAVEMFEAIGQGHIKAVWIAGTNPVDSMPEADRVRAALAACPF
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
VVVSDVLADTDTTRVAHVLLPAAAWGEKDGTVTNSERRISRQRPFLPLPGSARPDWDIMA
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHH
DVARRMGFAEGFSYTTAAEVFAEHAALSAFENDGERDFDIGAHAGIDAVGYEALAPFQWP
HHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCHHHCCCCCCH
QRRGAVTGTERFFAAGGFFTPEGKGRFVAPREARAGTGPLARLPAQEPTDQGFILNTGRV
HHCCCCCCCHHHEEECCEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
RDHWHTMTRTGKSPRLSQHMAEPFVEIHPDDAVQSGIAEASLVRLSKGPAQAIARALITN
HHHHHHHHHCCCCCCHHHHHHCCCEEECCCHHHHHCCHHHHHHHCCCCHHHHHHHHHHHC
RQRRGSVFVPMHWNDRFASNARIDALVDAVTDPVSGQPALKQARASLASFNAAAYGFAVV
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHEEEEE
ARKPADIPADYWAIARCTGGWRLELAFGQAKDDWHAFALRLFGGGEHVDILAYNDVGAGR
ECCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCEEEEEEEECCCCEEEEEEECCCCCCE
YRIAAFDGAALLGALFIAPQPVGVARGWLVEQLATSHDLQRQRLAVLATRPSAGQSDKGA
EEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCE
IVCSCFGVGSNEIAAAIASGCRTVKAVGDATQAGTNCGSCRAEIKGIIDAKHLQAAE
EEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATGAAHDVKTTCPYCGVGCGVIATVTPDGSVSVSGDPEHPANFGRLCSKGSALAETIDL
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHCCCCHHHHHHCC
DGRLLRPHIDGRPADWDEALDLVAARFASTIAEHGPDSVAFYVSGQLLTEDYYVANKLMK
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEECHHHHHHHHHHH
GFIGSANIDTNSRLCMASSVAGHRRAFGTDTVPGNYEDLELADLIVLVGSNLAWCHPVLY
HHCCCCCCCCCCCEEEEHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
QRIAAEKERRPSLKIVVIDPRRTMTADIADMHLAISPDGDVALFGGLLSWIANTGALNYS
HHHHHHHHCCCCEEEEEECCCCCCEECCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCHH
YISNFTTGAAQALRFGAQDLRAVSRKCGIAPSQLIEFYSLFTRTERTVTVYSQGVNQSET
HHHCCCHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GTDKVNAIINCHLATGRIGKPGASPFSVTGQPNAMGGREVGGMANMLAAHMDIENETHRA
CCCCEEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCHHCHHHHHHHHHCCCCCHHHHH
RVQRFWQAPTVATRPGRKAVEMFEAIGQGHIKAVWIAGTNPVDSMPEADRVRAALAACPF
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
VVVSDVLADTDTTRVAHVLLPAAAWGEKDGTVTNSERRISRQRPFLPLPGSARPDWDIMA
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHH
DVARRMGFAEGFSYTTAAEVFAEHAALSAFENDGERDFDIGAHAGIDAVGYEALAPFQWP
HHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCHHHCCCCCCH
QRRGAVTGTERFFAAGGFFTPEGKGRFVAPREARAGTGPLARLPAQEPTDQGFILNTGRV
HHCCCCCCCHHHEEECCEECCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
RDHWHTMTRTGKSPRLSQHMAEPFVEIHPDDAVQSGIAEASLVRLSKGPAQAIARALITN
HHHHHHHHHCCCCCCHHHHHHCCCEEECCCHHHHHCCHHHHHHHCCCCHHHHHHHHHHHC
RQRRGSVFVPMHWNDRFASNARIDALVDAVTDPVSGQPALKQARASLASFNAAAYGFAVV
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHEEEEE
ARKPADIPADYWAIARCTGGWRLELAFGQAKDDWHAFALRLFGGGEHVDILAYNDVGAGR
ECCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCEEEEEEEECCCCEEEEEEECCCCCCE
YRIAAFDGAALLGALFIAPQPVGVARGWLVEQLATSHDLQRQRLAVLATRPSAGQSDKGA
EEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCE
IVCSCFGVGSNEIAAAIASGCRTVKAVGDATQAGTNCGSCRAEIKGIIDAKHLQAAE
EEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]