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The map label for this gene is csaA [H]

Identifier: 152977327

GI number: 152977327

Start: 3710552

End: 3710884

Strand: Reverse

Name: csaA [H]

Synonym: Bcer98_3652

Alternate gene names: 152977327

Gene position: 3710884-3710552 (Counterclockwise)

Preceding gene: 152977328

Following gene: 152977326

Centisome position: 90.8

GC content: 40.84

Gene sequence:

>333_bases
ATGGCAACGATTGAAGACTTTATGAAATTAGATATCCGCATCGGAACGATTGTAGAAGCAGAGCTATTTCCAGAAGCGCG
GAAGCCGGCGATAAAATTAAAAGTTGATTTTGGAGAAGAAGTGGGGATGAAACAATCTTCAGCCCAAATTACGAAAAGAT
ATGAGCCGGAAGAGTTAATTGGCAGACAGGTCATTGGAGTTGTGAACTTTCCACCAAGACGAATTGCGGGATTTAAATCA
GAGGTGTTAATAATAGGTGGTGTTCCAGAAGAAGGAGATGTTGTGCTTTTAAAACCTGATGAGCTAGTGCCAAATGGAAC
ACCAATTGCATAA

Upstream 100 bases:

>100_bases
ATATGACATAGAGATAAAAATAGCTGATTTTTGGTGAAAAATCTTTTATTTCATTTGTTTAAAATGTAGGTAGGTATTTC
AAAAATTGGAGGGAGCATAT

Downstream 100 bases:

>100_bases
AATAATAAGCAAATTATACTACAAACTGTTGTAAGAATAAGGGACTTGCTTGATATAAATAAAATTTCACCAACCAGGCT
GTTACTGCCCTAAAAGAGCC

Product: export-related chaperone CsaA

Products: AMP; diphosphate +L-methionyl-tRNAMet [C]

Alternate protein names: NA

Number of amino acids: Translated: 110; Mature: 109

Protein sequence:

>110_residues
MATIEDFMKLDIRIGTIVEAELFPEARKPAIKLKVDFGEEVGMKQSSAQITKRYEPEELIGRQVIGVVNFPPRRIAGFKS
EVLIIGGVPEEGDVVLLKPDELVPNGTPIA

Sequences:

>Translated_110_residues
MATIEDFMKLDIRIGTIVEAELFPEARKPAIKLKVDFGEEVGMKQSSAQITKRYEPEELIGRQVIGVVNFPPRRIAGFKS
EVLIIGGVPEEGDVVLLKPDELVPNGTPIA
>Mature_109_residues
ATIEDFMKLDIRIGTIVEAELFPEARKPAIKLKVDFGEEVGMKQSSAQITKRYEPEELIGRQVIGVVNFPPRRIAGFKSE
VLIIGGVPEEGDVVLLKPDELVPNGTPIA

Specific function: Could serves as a molecular chaperone for exported proteins or alternatively acts by stabilizing the secA protein [H]

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR008231
- InterPro:   IPR002547 [H]

Pfam domain/function: PF01588 tRNA_bind [H]

EC number: 6.1.1.10 [C]

Molecular weight: Translated: 12101; Mature: 11970

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIEDFMKLDIRIGTIVEAELFPEARKPAIKLKVDFGEEVGMKQSSAQITKRYEPEELI
CCCHHHHHEEEEEECEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHCCCCHHHHH
GRQVIGVVNFPPRRIAGFKSEVLIIGGVPEEGDVVLLKPDELVPNGTPIA
CHHEEEEECCCCHHHCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ATIEDFMKLDIRIGTIVEAELFPEARKPAIKLKVDFGEEVGMKQSSAQITKRYEPEELI
CCHHHHHEEEEEECEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHCCCCHHHHH
GRQVIGVVNFPPRRIAGFKSEVLIIGGVPEEGDVVLLKPDELVPNGTPIA
CHHEEEEECCCCHHHCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mn2+; Zn2+ [C]

Kcat value (1/min): 198 [C]

Specific activity: 66.7

Km value (mM): 0.55 {ATP}} 0.41 {ATP}} 0.0009 {tRNA}} 0.48 {Met}} 0.078 {Met}} 0.022 {Met}} 0.02 {Met}} [C]

Substrates: ATP; @METH01.txt*L-methionine!; tRNAMet [C]

Specific reaction: ATP + @METH01.txt*L-methionine! + tRNAMet = AMP + diphosphate +L-methionyl-tRNAMet [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Dipyridyl; Adenosine; NH4+; Periodate-oxidized ATP; Periodate-oxidized tRNA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1435734; 9384377 [H]