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The map label for this gene is sigK [H]

Identifier: 152976769

GI number: 152976769

Start: 3109821

End: 3110534

Strand: Reverse

Name: sigK [H]

Synonym: Bcer98_3065

Alternate gene names: 152976769

Gene position: 3110534-3109821 (Counterclockwise)

Preceding gene: 152976770

Following gene: 152976768

Centisome position: 76.11

GC content: 37.82

Gene sequence:

>714_bases
TTGAGTTTATTCGCCGCAATTGGATATATGGTTCGTGAAGTGTTTGTGTTTGTTTCTTATGTGAAAAACAATGCGTTTCC
ACAACCATTGTCACCAGATGACGAAAAAAAGTACTTAGAGTTAATGGAGCAAGGGGATGCTCAAGCGAGAAACTTATTGA
TTGAACATAACTTGCGGCTTGTGGCCCACATTGTTAAGAAATTTGAAAACACAGGTGAAGATGCAGAAGATTTAATCTCA
ATCGGCACAATTGGGCTTATTAAAGCGATTGAAAGCTATTCAGCAGGGAAAGGAACAAAGCTTGCAACATATGCAGCACG
CTGTATTGAAAATGAAATTTTAATGCATCTCCGCGTTTTGAAGAAAACAAAAAAGGATGTTTCACTTCATGACCCGATTG
GGCAAGATAAAGAGGGTAATGAAATATCACTTATCGATATTTTGAAATCTGAATCAGAAGACGTGATCGATATGATTCAA
CTTAGTATGGAGTTAGAAAAAATTAAAGAGTACATTGATATTTTAGATGAGAGGGAGAAAGAAGTTATTGTAAAGCGTTT
TGGACTCGGACTGGATAAGGAGAAAACGCAGCGAGAAATCGCAAAGGCGCTTGGGATTTCCAGGAGCTATGTATCAAGAA
TTGAGAAGCGAGCACTGATGAAAATGTTTCATGAATTTGTGAGGGCGGAGAAAGAGATGAAAGCAAAGGAATAA

Upstream 100 bases:

>100_bases
TTTTAATGATGAATTTTCATATTTAAATGGGTACAGACACAAACAACTTCACGCCCACATACATATAGTAAAGACCACTA
TTTTGGCGGGGGTGAAGGGT

Downstream 100 bases:

>100_bases
GAAAAAGGAACGACTGCATTGATACGGCAGTCGTTCCTTTTTTCTTTTGATGCAGGTGATAAAAAGCGAAGCGAAAAGAA
GAAATATATGTTCACTGTTG

Product: sporulation sigma factor SigK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSLFAAIGYMVREVFVFVSYVKNNAFPQPLSPDDEKKYLELMEQGDAQARNLLIEHNLRLVAHIVKKFENTGEDAEDLIS
IGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVLKKTKKDVSLHDPIGQDKEGNEISLIDILKSESEDVIDMIQ
LSMELEKIKEYIDILDEREKEVIVKRFGLGLDKEKTQREIAKALGISRSYVSRIEKRALMKMFHEFVRAEKEMKAKE

Sequences:

>Translated_237_residues
MSLFAAIGYMVREVFVFVSYVKNNAFPQPLSPDDEKKYLELMEQGDAQARNLLIEHNLRLVAHIVKKFENTGEDAEDLIS
IGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVLKKTKKDVSLHDPIGQDKEGNEISLIDILKSESEDVIDMIQ
LSMELEKIKEYIDILDEREKEVIVKRFGLGLDKEKTQREIAKALGISRSYVSRIEKRALMKMFHEFVRAEKEMKAKE
>Mature_236_residues
SLFAAIGYMVREVFVFVSYVKNNAFPQPLSPDDEKKYLELMEQGDAQARNLLIEHNLRLVAHIVKKFENTGEDAEDLISI
GTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVLKKTKKDVSLHDPIGQDKEGNEISLIDILKSESEDVIDMIQL
SMELEKIKEYIDILDEREKEVIVKRFGLGLDKEKTQREIAKALGISRSYVSRIEKRALMKMFHEFVRAEKEMKAKE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=256, Percent_Identity=24.21875, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27122; Mature: 26991

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFAAIGYMVREVFVFVSYVKNNAFPQPLSPDDEKKYLELMEQGDAQARNLLIEHNLRL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAHIVKKFENTGEDAEDLISIGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KKTKKDVSLHDPIGQDKEGNEISLIDILKSESEDVIDMIQLSMELEKIKEYIDILDEREK
HHHHHCCCCCCCCCCCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVIVKRFGLGLDKEKTQREIAKALGISRSYVSRIEKRALMKMFHEFVRAEKEMKAKE
HHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLFAAIGYMVREVFVFVSYVKNNAFPQPLSPDDEKKYLELMEQGDAQARNLLIEHNLRL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAHIVKKFENTGEDAEDLISIGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KKTKKDVSLHDPIGQDKEGNEISLIDILKSESEDVIDMIQLSMELEKIKEYIDILDEREK
HHHHHCCCCCCCCCCCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVIVKRFGLGLDKEKTQREIAKALGISRSYVSRIEKRALMKMFHEFVRAEKEMKAKE
HHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]