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The map label for this gene is thiM

Identifier: 151222208

GI number: 151222208

Start: 2217608

End: 2218399

Strand: Reverse

Name: thiM

Synonym: NWMN_1996

Alternate gene names: 151222208

Gene position: 2218399-2217608 (Counterclockwise)

Preceding gene: 151222209

Following gene: 151222207

Centisome position: 77.06

GC content: 35.35

Gene sequence:

>792_bases
ATGAATTATCTAAATAAAATACGTATTGAAAACCCATTAACAATTTGTTATACAAACGATGTAGTTAAAAATTTTACAGC
GAATGGTTTATTAAGTATTGGTGCTAGCCCTGCAATGAGTGAAGCTCCCGAAGAAGCTGAAGAATTTTACAAAGTTGCAC
AAGCGCTATTAATCAATATCGGTACTTTAACAGCAGAAAATGAACAAGATATTATTGCGATTGCTCAAACGGCAAATGAG
GCAGGCTTACCTATTGTATTTGACCCTGTAGCTGTTGGTGCTTCTACATATCGAAAGCAATTTTGTAAATTATTATTGAA
ATCAGCGAAAGTATCAGTAATTAAAGGCAATGCATCTGAAATATTAGCGTTGATTGATGATACAGCAACTATGAAAGGTA
CAGATAGTGATGCTAATCTTGATGCGGTTGCAATAGCGAAAAAGGCTTACGCAACATATAAAACTGCAATAGTAATCACA
GGTAAAGAGGACGTTATTGTTCAAGATAATAAAGCCTTCGTATTAGCTAATGGATCTCCATTATTAGCACGAGTAACTGG
AGCTGGTTGTTTATTAGGAGGCGTTATTGCTGGATTTTTATTTAGAGAAACAGAACCAGACATAGAAGCGTTAATTGAAG
CGGTAAGCGTATTTAATATTGCTGCTGAGGTAGCTGCTGAAAATGAAAATTGTGGTGGTCCTGGTACGTTTTCACCATTG
TTGCTTGATACGTTATATCATTTAAATGAAACAACCTATCAACAACGGATTCGTATTCAAGAGGTGGAATAA

Upstream 100 bases:

>100_bases
AAAAAGTTTATTTCAATGAGTATACAATATACGCCTGAAATCGGCCGTGGTAGAGGTCCAGTGAATCATTTTGCATATTT
AAAGAAAGAGGGATTAGACG

Downstream 100 bases:

>100_bases
TATGTTTAACCAATCGTATCTAAATGTGTATTTTATTTGTGGGACCTCCGATGTTCCGAGTCATCGAACTATTCATGAAG
TATTAGAAGCAGCATTAAAG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANE
AGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
LLDTLYHLNETTYQQRIRIQEVE

Sequences:

>Translated_263_residues
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANE
AGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
LLDTLYHLNETTYQQRIRIQEVE
>Mature_263_residues
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANE
AGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
LLDTLYHLNETTYQQRIRIQEVE

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=250, Percent_Identity=38.8, Blast_Score=158, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6325042, Length=275, Percent_Identity=31.6363636363636, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 28090; Mature: 28090

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINI
CCCCCCEEECCCEEEEEECHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
GTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASE
CCEECCCCCCEEEEEECCCCCCCCEEECHHHHCHHHHHHHHHHHHHHHCCEEEEECCHHH
ILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVITGKEDVIVQDNKAFVLANGSP
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHEEEEEEEEECCCCEEEECCCEEEEECCCC
LLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
EEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LLDTLYHLNETTYQQRIRIQEVE
HHHHHHHCCCHHHHHHEEEEECC
>Mature Secondary Structure
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINI
CCCCCCEEECCCEEEEEECHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
GTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASE
CCEECCCCCCEEEEEECCCCCCCCEEECHHHHCHHHHHHHHHHHHHHHCCEEEEECCHHH
ILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVITGKEDVIVQDNKAFVLANGSP
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHEEEEEEEEECCCCEEEECCCEEEEECCCC
LLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
EEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LLDTLYHLNETTYQQRIRIQEVE
HHHHHHHCCCHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA