Click here to switch to the map view.

The map label for this gene is thiD

Identifier: 151222209

GI number: 151222209

Start: 2218383

End: 2219213

Strand: Reverse

Name: thiD

Synonym: NWMN_1997

Alternate gene names: 151222209

Gene position: 2219213-2218383 (Counterclockwise)

Preceding gene: 151222210

Following gene: 151222208

Centisome position: 77.09

GC content: 35.74

Gene sequence:

>831_bases
ATGATTAAACCTAAAATAGCATTAACCATTGCAGGTACTGATCCAACAGGTGGTGCCGGCGTAATGGCTGATTTAAAATC
ATTTCATTCATGTGGTGTATATGGTATGGGCGTCGTTACAAGTATTGTTGCTCAAAATACATTGGGCGTACAACATATTC
ATAATTTAAATCATCAATGGGTAGATGAACAACTTGATAGTGTCTTCAATGATACCTTACCTCATGCTATTAAAACGGGG
ATGATTGCTACAGCAGATACTATGGAAACGATTCGTCATTATTTAATGCAACATGAATCTATTCCATATGTAATTGATCC
TGTTATGTTGGCGAAAAGCGGTGATTCATTAATGGATAATGACACAAAGCAAAACTTGCAGCATACGTTATTGCCATTAG
CTGACGTAGTAACACCGAATTTACCAGAAGCTGAAGAAATAACGGGACTAACCATTGATAGTGAAGAAAAAATTATGCAG
GCTGGCCGCATCTTTATTAATGAGATTGGTAGTAAAGGTGTCATCATTAAAGGCGGTCATTCAAATGATACTGATATAGC
AAAAGATTATTTATTTACTAACGAAGGTGTTCAAACATTTGAAAATGAACGATTTAAAACAAAACATACGCATGGAACAG
GGTGTACATTTTCAGCAGTTATAACGGCAGAACTTGCAAAAGGTAGACCATTATTTGAGGCTGTACACAAGGCTAAAAAG
TTTATTTCAATGAGTATACAATATACGCCTGAAATCGGCCGTGGTAGAGGTCCAGTGAATCATTTTGCATATTTAAAGAA
AGAGGGATTAGACGATGAATTATCTAAATAA

Upstream 100 bases:

>100_bases
GAACAAGTAAAGCAAGTATTTTTAGAAAGTTGCATACATGAGCGTCGATTTTTCAATATGGCTATGACACTAGAACAATG
GGAATTTGGAGGAAAAGTAA

Downstream 100 bases:

>100_bases
AATACGTATTGAAAACCCATTAACAATTTGTTATACAAACGATGTAGTTAAAAATTTTACAGCGAATGGTTTATTAAGTA
TTGGTGCTAGCCCTGCAATG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTG
MIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKK
FISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK

Sequences:

>Translated_276_residues
MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTG
MIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKK
FISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
>Mature_276_residues
MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTG
MIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKK
FISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=259, Percent_Identity=43.2432432432432, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788758, Length=181, Percent_Identity=24.8618784530387, Blast_Score=61, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6325378, Length=269, Percent_Identity=30.4832713754647, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324997, Length=270, Percent_Identity=34.4444444444444, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6324517, Length=268, Percent_Identity=32.8358208955224, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 30229; Mature: 30229

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQW
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHCCHHH
VDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECHHHEECCCCCHHCC
DTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQAGRIFINEIGSKGVIIKGGH
CHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEECCHHHHHHHHHHHHHHCCCCCEEEECCC
SNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKK
CCCHHHHHHHHHCCCCHHHHHCCCCEECCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHH
FISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
HHHEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure
MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQW
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHCCHHH
VDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECHHHEECCCCCHHCC
DTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQAGRIFINEIGSKGVIIKGGH
CHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEECCHHHHHHHHHHHHHHCCCCCEEEECCC
SNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKK
CCCHHHHHHHHHCCCCHHHHHCCCCEECCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHH
FISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
HHHEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]