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The map label for this gene is thiE

Identifier: 151222207

GI number: 151222207

Start: 2216965

End: 2217606

Strand: Reverse

Name: thiE

Synonym: NWMN_1995

Alternate gene names: 151222207

Gene position: 2217606-2216965 (Counterclockwise)

Preceding gene: 151222208

Following gene: 151222206

Centisome position: 77.03

GC content: 35.51

Gene sequence:

>642_bases
ATGTTTAACCAATCGTATCTAAATGTGTATTTTATTTGTGGGACCTCCGATGTTCCGAGTCATCGAACTATTCATGAAGT
ATTAGAAGCAGCATTAAAGGCAGGGATTACACTTTTCCAATTTCGAGAAAAGGGTGAATCAGCTTTAAAAGGAAACGATA
AACTTGTATTAGCTAAAGAATTACAGCATCTCTGTCATCAATATGATGTGCCTTTTATCGTTAATGATGATGTATCCTTG
GCAAAAGAAATTAACGCGGATGGTATACATGTCGGTCAAGATGATGCGAAAGTCAAAGAGATAGCACAATATTTCACTGA
TAAAATTATTGGACTTAGTATTAGTGATTTAGATGAGTATGCAAAATCTGATTTAACTCATGTTGATTATATAGGTGTTG
GACCAATCTATCCTACGCCGTCGAAGCATGACGCGCATATACCAGTAGGTCCAGAAATGATTGCAACGTTTAAAGAAATG
AATCCGCAACTACCGATTGTAGCTATTGGCGGTATCAATACTAATAATGTGGCGCCGATTGTAGAAGCAGGTGCCAATGG
AATTTCAGTCATTTCAGCCATTTCTAAAAGTGAAAATATTGAAAAGACTGTTAATCGATTCAAAGATTTTTTTAATAATT
AG

Upstream 100 bases:

>100_bases
GGTGGTCCTGGTACGTTTTCACCATTGTTGCTTGATACGTTATATCATTTAAATGAAACAACCTATCAACAACGGATTCG
TATTCAAGAGGTGGAATAAT

Downstream 100 bases:

>100_bases
TTAAATTTTTCCACTTATTGTTTCATACGTACTCATTAATGTGATAGAATAAATCCTATGTGAATATAATGATAGAGGTG
GAAAAATGAAGAAAAAAGCG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSL
AKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN

Sequences:

>Translated_213_residues
MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSL
AKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN
>Mature_213_residues
MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSL
AKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=175, Percent_Identity=28, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325042, Length=223, Percent_Identity=30.4932735426009, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_STAA1 (A7X4S8)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442666.1
- ProteinModelPortal:   A7X4S8
- SMR:   A7X4S8
- STRING:   A7X4S8
- EnsemblBacteria:   EBSTAT00000005024
- GeneID:   5560378
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2076
- eggNOG:   COG0352
- GeneTree:   EBGT00050000024447
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- ProtClustDB:   PRK00043
- BioCyc:   SAUR418127:SAHV_2076-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23399; Mature: 23399

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKE
CCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCEEEHHH
LQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLDEY
HHHHHHHCCCCEEECCCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHH
AKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEMNPQLPIVAIGGINTNNVAPI
HHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCHH
VEAGANGISVISAISKSENIEKTVNRFKDFFNN
HHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKE
CCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCEEEHHH
LQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLDEY
HHHHHHHCCCCEEECCCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHH
AKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEMNPQLPIVAIGGINTNNVAPI
HHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCHH
VEAGANGISVISAISKSENIEKTVNRFKDFFNN
HHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA