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The map label for this gene is sigB

Identifier: 151222182

GI number: 151222182

Start: 2189575

End: 2190345

Strand: Reverse

Name: sigB

Synonym: NWMN_1970

Alternate gene names: 151222182

Gene position: 2190345-2189575 (Counterclockwise)

Preceding gene: 151222183

Following gene: 151222181

Centisome position: 76.08

GC content: 35.02

Gene sequence:

>771_bases
ATGGCGAAAGAGTCGAAATCAGCTAATGAAATTTCACCTGAGCAAATTAACCAATGGATTAAAGAACACCAAGAAAATAA
GAATACAGATGCACAGGATAAGTTAGTTAAACATTACCAAAAACTAATTGAGTCATTGGCATATAAATATTCTAAAGGAC
AATCACATCACGAAGATTTAGTTCAAGTTGGTATGGTTGGTTTAATAGGTGCCATAAATAGATTCGATATGTCCTTTGAA
CGGAAGTTTGAAGCCTTTTTAGTACCTACTGTAATCGGTGAAATCAAAAGATATCTACGAGATAAAACTTGGAGTGTACA
TGTTCCGAGACGTATTAAAGAAATTGGGCCAAGAATCAAAAAAGTGAGCGATGAACTAACCGCTGAATTAGAGCGTTCAC
CTTCTATCAGTGAAATAGCTGATCGATTAGAAGTCTCAGAAGAAGAAGTGTTAGAAGCAATGGAAATGGGACAAAGTTAT
AATGCGTTAAGTGTTGATCATTCCATTGAAGCTGATAAAGATGGTTCAACTGTTACGCTATTAGATATTATGGGGCAACA
AGATGACCATTATGACTTAACTGAAAAACGTATGATTTTAGAAAAAATATTACCTATATTATCTGATCGCGAACGAGAAA
TCATACAATGTACGTTTATTGAAGGATTGAGTCAAAAAGAGACAGGTGAGCGTATCGGTTTAAGTCAAATGCATGTATCA
CGACTTCAGAGAACGGCAATTAAGAAATTACAAGAAGCAGCACATCAATAG

Upstream 100 bases:

>100_bases
TTTTTAATCGAATCTTTAATGGATGAAGTCACAGTATATAAAGAATCTGGTGTGACAATCAGTATGACTAAGTATATAAA
AAAAGAGCAGGTGCGAAATA

Downstream 100 bases:

>100_bases
AATTTGTTTATTAATGATACGTTTTATAATGAAAAATCCATATAATTATCCCTTGATTATTAAATTGAAATCGAGGGGTA
TTTTTAATTTAATTAAGATT

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: General stress protein 84; GSP84; Sigma-37 [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIESLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFE
RKFEAFLVPTVIGEIKRYLRDKTWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCTFIEGLSQKETGERIGLSQMHVS
RLQRTAIKKLQEAAHQ

Sequences:

>Translated_256_residues
MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIESLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFE
RKFEAFLVPTVIGEIKRYLRDKTWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCTFIEGLSQKETGERIGLSQMHVS
RLQRTAIKKLQEAAHQ
>Mature_255_residues
AKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIESLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFER
KFEAFLVPTVIGEIKRYLRDKTWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSYN
ALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCTFIEGLSQKETGERIGLSQMHVSR
LQRTAIKKLQEAAHQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcr

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=22.2222222222222, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789098, Length=230, Percent_Identity=25.6521739130435, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29444; Mature: 29313

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIESLAYKYSKGQSHHEDL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDKTWSVHVPRRIKEIGPRIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHH
KVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSYNALSVDHSIEADKDGSTVTL
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEE
LDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCTFIEGLSQKETGERIGLSQMHVS
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCHHHHHHHHHHHH
RLQRTAIKKLQEAAHQ
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIESLAYKYSKGQSHHEDL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDKTWSVHVPRRIKEIGPRIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHH
KVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSYNALSVDHSIEADKDGSTVTL
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEE
LDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCTFIEGLSQKETGERIGLSQMHVS
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCHHHHHHHHHHHH
RLQRTAIKKLQEAAHQ
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3016731; 3027048; 2170324; 9384377; 9298659 [H]