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The map label for this gene is nusA [H]

Identifier: 150398625

GI number: 150398625

Start: 3644337

End: 3645995

Strand: Direct

Name: nusA [H]

Synonym: Smed_3439

Alternate gene names: 150398625

Gene position: 3644337-3645995 (Clockwise)

Preceding gene: 150398624

Following gene: 150398626

Centisome position: 96.36

GC content: 61.18

Gene sequence:

>1659_bases
ATGCGCCTGCAACAGACGGAGACAAGAGACATGGCAGTCAGTGCTAACCGGCTCGAACTTCTGCAGATCGCAGATGCTGT
GGCACGCGAAAAGGTGATCGACCGCGAGATCGTTCTGGCCGCGATGGCGGATGCGATCCAGAAGGCGGCTCGTTCGCGCT
ACGGTTCGGAATCGAACATCCGCGCCGACATCAATCCGAAGACCGGGGAAATCCGTCTCCAGCGCCTCCTGGAAGTGGTC
GAAAAGGCGGAAGACTATTCGACTCAGATTCCGATCGAACTCGCCCGCGATCGCAATCCCGATGCAAAGCTCGGCGATTT
CATCGCCGATCCGCTCCCGCCCATGGATTTTGGCCGCATCGCCGCTCAGTCGGCCAAGCAGGTTATCGTGCAGAAGGTGC
GCGAAGCCGAGCGCGACCGCCAGTACGACGAGTTCAAGGACCGTGTCGGCGAGATCGTCAACGGCACCGTCAAGCGCGTC
GAATATGGCAATGTCATCGTCGATCTCGGGCGCGGTGAAGGCATCATCCGGCGCGACGAGATGATCCCGCGCGAAAACAT
GCGTTACGGCGACCGCGTCCGTGCCTTTGTCTACGACGTGCGCCGCGAGCAACGCGGACCGCAGATATTCCTGTCGCGCA
CCCATCCGCAGTTCATGGTGAAGCTCTTCACCATGGAGGTACCGGAAATCTACGACGGCGTCATCCAGATCAAGTCGGTT
GCCCGCGATCCGGGCTCGCGCGCCAAGATCGCCGTCGTCTCGAACGATTCGTCGATCGATCCGGTCGGCGCCTGCGTCGG
CATGCGCGGCTCGCGCGTGCAGGCTGTCGTCGGTGAACTCCAGGGCGAAAAGATCGATATCATCCCGTGGTCGCCGGATC
CGGCTTCCTTCATCGTCAATGCGCTGCAGCCGGCGGAAGTGGCGAAGGTCGTTCTAGACGAGGATGCGGAGCGTATCGAA
GTCGTGGTTCCGGACGAGCAGCTTTCGCTCGCCATCGGCCGCCGCGGCCAGAACGTCCGTCTCGCCTCGCAGCTGACCGG
ATGGGATATCGATATCCTCACCGAACAGGAGGAGAGCGAGCGCCGTCAGAAGGAATTCAACGAGCGCACACAGCTCTTCA
TGGAAGCCCTGGACGTCGACGAGATGGTAGGCCAGGTGCTCGCCTCCGAAGGCTTTGCCCAGGTGGAAGAGCTCGCTTAT
GTCGATCTCGACGAAATTGCCTCCATCGAGGGCTTCGACGAGGAAACGTCGAACGAGATCCAGACCCGCGCCCGCGAATA
TCTCGAAAAGATCGAGGCGGAAATGGACGCCAAGCGCAAGGAACTCGGTGTTGCCGACGAGCTGCGCACGATCAATGGGC
TCAACAGCCAGATGCTGGTCGCTCTCGGCGAGGAAGGCATCAAGACGATAGAGGACTTTGCCGGCTGCGCCGCCGACGAC
CTCGTAGGCTGGGTCGAACGCAAGGATGGTGAGACCAAGCGCTTCGAGGGAACGTTCTCGAAGCTCGAGGTTACCCGGGA
AGAGGCCGAAGCGATGATCGTGCAGGCTCGTCTCGCTGCCGGCTGGATCACCGAAGAGGATCTGGCCAAACAACAGGAGG
AAGAGCCGGAACAGGATGAGACGATCGAAGTCGCCGAAGGCGCGGATCAGGACGCCTGA

Upstream 100 bases:

>100_bases
ACGCTCTGACAGCCGACAAGAAGGCGAAGGCCGCGCGCGCCGCCAACGAAAACTTCGAAGAAGAAGATAGAGATATCGAA
TAAAGCTGGGTGCGCTTTCG

Downstream 100 bases:

>100_bases
TCGCGATGATCGTCGAAATCGATGCCGACAGCCTCCCCGTGGACCGGGGTCCGAAGGACAGGGGCCCGAAGGGCGGGAGC
GCGAAGGACGGGGACGGCAA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MRLQQTETRDMAVSANRLELLQIADAVAREKVIDREIVLAAMADAIQKAARSRYGSESNIRADINPKTGEIRLQRLLEVV
EKAEDYSTQIPIELARDRNPDAKLGDFIADPLPPMDFGRIAAQSAKQVIVQKVREAERDRQYDEFKDRVGEIVNGTVKRV
EYGNVIVDLGRGEGIIRRDEMIPRENMRYGDRVRAFVYDVRREQRGPQIFLSRTHPQFMVKLFTMEVPEIYDGVIQIKSV
ARDPGSRAKIAVVSNDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSPDPASFIVNALQPAEVAKVVLDEDAERIE
VVVPDEQLSLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQKEFNERTQLFMEALDVDEMVGQVLASEGFAQVEELAY
VDLDEIASIEGFDEETSNEIQTRAREYLEKIEAEMDAKRKELGVADELRTINGLNSQMLVALGEEGIKTIEDFAGCAADD
LVGWVERKDGETKRFEGTFSKLEVTREEAEAMIVQARLAAGWITEEDLAKQQEEEPEQDETIEVAEGADQDA

Sequences:

>Translated_552_residues
MRLQQTETRDMAVSANRLELLQIADAVAREKVIDREIVLAAMADAIQKAARSRYGSESNIRADINPKTGEIRLQRLLEVV
EKAEDYSTQIPIELARDRNPDAKLGDFIADPLPPMDFGRIAAQSAKQVIVQKVREAERDRQYDEFKDRVGEIVNGTVKRV
EYGNVIVDLGRGEGIIRRDEMIPRENMRYGDRVRAFVYDVRREQRGPQIFLSRTHPQFMVKLFTMEVPEIYDGVIQIKSV
ARDPGSRAKIAVVSNDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSPDPASFIVNALQPAEVAKVVLDEDAERIE
VVVPDEQLSLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQKEFNERTQLFMEALDVDEMVGQVLASEGFAQVEELAY
VDLDEIASIEGFDEETSNEIQTRAREYLEKIEAEMDAKRKELGVADELRTINGLNSQMLVALGEEGIKTIEDFAGCAADD
LVGWVERKDGETKRFEGTFSKLEVTREEAEAMIVQARLAAGWITEEDLAKQQEEEPEQDETIEVAEGADQDA
>Mature_552_residues
MRLQQTETRDMAVSANRLELLQIADAVAREKVIDREIVLAAMADAIQKAARSRYGSESNIRADINPKTGEIRLQRLLEVV
EKAEDYSTQIPIELARDRNPDAKLGDFIADPLPPMDFGRIAAQSAKQVIVQKVREAERDRQYDEFKDRVGEIVNGTVKRV
EYGNVIVDLGRGEGIIRRDEMIPRENMRYGDRVRAFVYDVRREQRGPQIFLSRTHPQFMVKLFTMEVPEIYDGVIQIKSV
ARDPGSRAKIAVVSNDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSPDPASFIVNALQPAEVAKVVLDEDAERIE
VVVPDEQLSLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQKEFNERTQLFMEALDVDEMVGQVLASEGFAQVEELAY
VDLDEIASIEGFDEETSNEIQTRAREYLEKIEAEMDAKRKELGVADELRTINGLNSQMLVALGEEGIKTIEDFAGCAADD
LVGWVERKDGETKRFEGTFSKLEVTREEAEAMIVQARLAAGWITEEDLAKQQEEEPEQDETIEVAEGADQDA

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=464, Percent_Identity=44.6120689655172, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 61997; Mature: 61997

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQQTETRDMAVSANRLELLQIADAVAREKVIDREIVLAAMADAIQKAARSRYGSESNI
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
RADINPKTGEIRLQRLLEVVEKAEDYSTQIPIELARDRNPDAKLGDFIADPLPPMDFGRI
EEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCHHHH
AAQSAKQVIVQKVREAERDRQYDEFKDRVGEIVNGTVKRVEYGNVIVDLGRGEGIIRRDE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHEEEECCEEEECCCCCCEEECCC
MIPRENMRYGDRVRAFVYDVRREQRGPQIFLSRTHPQFMVKLFTMEVPEIYDGVIQIKSV
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHH
ARDPGSRAKIAVVSNDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSPDPASFIVN
HCCCCCCEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHH
ALQPAEVAKVVLDEDAERIEVVVPDEQLSLAIGRRGQNVRLASQLTGWDIDILTEQEESE
HCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCEEEHHHCCCCCEEEECCCHHHH
RRQKEFNERTQLFMEALDVDEMVGQVLASEGFAQVEELAYVDLDEIASIEGFDEETSNEI
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCCCCCHHHHHH
QTRAREYLEKIEAEMDAKRKELGVADELRTINGLNSQMLVALGEEGIKTIEDFAGCAADD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCCCHHH
LVGWVERKDGETKRFEGTFSKLEVTREEAEAMIVQARLAAGWITEEDLAKQQEEEPEQDE
HHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
TIEVAEGADQDA
EEEHHCCCCCCC
>Mature Secondary Structure
MRLQQTETRDMAVSANRLELLQIADAVAREKVIDREIVLAAMADAIQKAARSRYGSESNI
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
RADINPKTGEIRLQRLLEVVEKAEDYSTQIPIELARDRNPDAKLGDFIADPLPPMDFGRI
EEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCHHHH
AAQSAKQVIVQKVREAERDRQYDEFKDRVGEIVNGTVKRVEYGNVIVDLGRGEGIIRRDE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHEEEECCEEEECCCCCCEEECCC
MIPRENMRYGDRVRAFVYDVRREQRGPQIFLSRTHPQFMVKLFTMEVPEIYDGVIQIKSV
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHH
ARDPGSRAKIAVVSNDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSPDPASFIVN
HCCCCCCEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHH
ALQPAEVAKVVLDEDAERIEVVVPDEQLSLAIGRRGQNVRLASQLTGWDIDILTEQEESE
HCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCEEEHHHCCCCCEEEECCCHHHH
RRQKEFNERTQLFMEALDVDEMVGQVLASEGFAQVEELAYVDLDEIASIEGFDEETSNEI
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCCCCCHHHHHH
QTRAREYLEKIEAEMDAKRKELGVADELRTINGLNSQMLVALGEEGIKTIEDFAGCAADD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCCCHHH
LVGWVERKDGETKRFEGTFSKLEVTREEAEAMIVQARLAAGWITEEDLAKQQEEEPEQDE
HHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
TIEVAEGADQDA
EEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]