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The map label for this gene is mltB [H]

Identifier: 150398622

GI number: 150398622

Start: 3640392

End: 3641630

Strand: Direct

Name: mltB [H]

Synonym: Smed_3436

Alternate gene names: 150398622

Gene position: 3640392-3641630 (Clockwise)

Preceding gene: 150398621

Following gene: 150398624

Centisome position: 96.26

GC content: 64.57

Gene sequence:

>1239_bases
ATGCTGAGATTCCTTTCCGTCCTGTTCCTGGCCAGCCTGTTCTTTGTCTCCGCCTCCTTCGCTGCGTCCAGGTCTGACGT
CGAAGCGCAGTTCCGCCAATGGCTTCAAAGCGATCTCTGGCCGGAAGCGCAGAAAGCCGGCGTATCGAGCGGCGCCTTTA
AAGCGGCGTTCAAGGGCGTAAGGCTCAATTGGGATCTGCCCGACCTCGCGCCGCCCGGCTTCCCCAAGCCGAAGCAGCGC
AAGCAGAGCCAGGCGGAGTTTTCTTCCCCCGGCTCCTATTTCTCGGAAAAGCGCCTGCAGGGTCTCGCTGCGACAGGCCG
CAGTCTTGCCTCTACCCACGCCTCCACCCTGAAGCGCATTGAAAGGACCTATGGCGTTCCCGGTACGATCGTCCTGGCCA
TCTGGGGCAAGGAGTCCGGCTTCGGCCGCGCCAAAATCCCGCACCCGATCATGGATGTGCTGGCGACCAAGGCCTTCATG
TCGACGCGGCCGGAGCTTTTCCGGCGCGAACTGATCGCGGCGCTCCATATTCTAGACAGCGGTGACGTCAAGGAGGCCGA
GATGCGCGGCTCCTGGGCCGGCGCCATGGGCCAGCCGCAGTTCCTGCCTACGAGCTTCCTGAAATATGCCGTCGATTTCG
ACGGGGACGGACGCCGCGACATCTGGAATTCCGTACCGGACAGCCTCGCCTCGATCGCCAGTTATCTGGCGCAGAAGGGC
TGGCAGCGCGGGCGCGACTGGGGTTTCGAGGTCGCGATCCCGTCGGGCGTCTCCTGTGCCCAGGAGGGTCCGGACCTTGC
TCGCCCGATCGCCGAATGGGCCGGCATGGGGATCGGCCGCGTATCGGGCAAGCCCTTCCCGGCGGCAGAACGCGGGGCCT
CAGGCATGATGCTGGTCCCGGCGGGCACGCATGGCCCGCAATTCATCGTTACGCCGAACTTCTACGTGATCAAGGAATAC
AACAATTCCGACCTCTACGCGCTCTATATAGGCAATCTCGCGGATCGCATCGCGACCGGCGGCGGCGCCTTCCGGGGCAA
GTGGGGCGACGTCGGCGGCATGTTGCGGTCCGATGTGCTCGGCCTGCAGAAGGCCCTGGTCGCGAAGGGCTACGACGTCG
GCAAGGCCGACGGGCTGCCGGGCTACAAGACCCGCCGCTCGCTCGGCGACTGGCAGGCGAAGAACGGCCTCAAGCCCACC
TGCTTTCCGGATGTTTCGCTGAAAGCAAAGCTGCGGTGA

Upstream 100 bases:

>100_bases
GCGAGCTGGACTATCTTGACGAGGGAACGCTCGCTGCCGCGCTGAGAGCGAGAACGGCGATCTGAGAATTCGCCTCAAGC
TTCGATAACAAGGAAAAGAG

Downstream 100 bases:

>100_bases
CCTTCGGTTGGGCGCGCGCGTCCGCCCCTCAACCGGCCTGCCTGCCACCTTCTCCCCGCAAGCGGGGCGAAGGAGACTCG
CGGCGCCACCTGATCTTCAC

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MLRFLSVLFLASLFFVSASFAASRSDVEAQFRQWLQSDLWPEAQKAGVSSGAFKAAFKGVRLNWDLPDLAPPGFPKPKQR
KQSQAEFSSPGSYFSEKRLQGLAATGRSLASTHASTLKRIERTYGVPGTIVLAIWGKESGFGRAKIPHPIMDVLATKAFM
STRPELFRRELIAALHILDSGDVKEAEMRGSWAGAMGQPQFLPTSFLKYAVDFDGDGRRDIWNSVPDSLASIASYLAQKG
WQRGRDWGFEVAIPSGVSCAQEGPDLARPIAEWAGMGIGRVSGKPFPAAERGASGMMLVPAGTHGPQFIVTPNFYVIKEY
NNSDLYALYIGNLADRIATGGGAFRGKWGDVGGMLRSDVLGLQKALVAKGYDVGKADGLPGYKTRRSLGDWQAKNGLKPT
CFPDVSLKAKLR

Sequences:

>Translated_412_residues
MLRFLSVLFLASLFFVSASFAASRSDVEAQFRQWLQSDLWPEAQKAGVSSGAFKAAFKGVRLNWDLPDLAPPGFPKPKQR
KQSQAEFSSPGSYFSEKRLQGLAATGRSLASTHASTLKRIERTYGVPGTIVLAIWGKESGFGRAKIPHPIMDVLATKAFM
STRPELFRRELIAALHILDSGDVKEAEMRGSWAGAMGQPQFLPTSFLKYAVDFDGDGRRDIWNSVPDSLASIASYLAQKG
WQRGRDWGFEVAIPSGVSCAQEGPDLARPIAEWAGMGIGRVSGKPFPAAERGASGMMLVPAGTHGPQFIVTPNFYVIKEY
NNSDLYALYIGNLADRIATGGGAFRGKWGDVGGMLRSDVLGLQKALVAKGYDVGKADGLPGYKTRRSLGDWQAKNGLKPT
CFPDVSLKAKLR
>Mature_412_residues
MLRFLSVLFLASLFFVSASFAASRSDVEAQFRQWLQSDLWPEAQKAGVSSGAFKAAFKGVRLNWDLPDLAPPGFPKPKQR
KQSQAEFSSPGSYFSEKRLQGLAATGRSLASTHASTLKRIERTYGVPGTIVLAIWGKESGFGRAKIPHPIMDVLATKAFM
STRPELFRRELIAALHILDSGDVKEAEMRGSWAGAMGQPQFLPTSFLKYAVDFDGDGRRDIWNSVPDSLASIASYLAQKG
WQRGRDWGFEVAIPSGVSCAQEGPDLARPIAEWAGMGIGRVSGKPFPAAERGASGMMLVPAGTHGPQFIVTPNFYVIKEY
NNSDLYALYIGNLADRIATGGGAFRGKWGDVGGMLRSDVLGLQKALVAKGYDVGKADGLPGYKTRRSLGDWQAKNGLKPT
CFPDVSLKAKLR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=231, Percent_Identity=31.6017316017316, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 44718; Mature: 44718

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFLSVLFLASLFFVSASFAASRSDVEAQFRQWLQSDLWPEAQKAGVSSGAFKAAFKGV
CHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHCCC
RLNWDLPDLAPPGFPKPKQRKQSQAEFSSPGSYFSEKRLQGLAATGRSLASTHASTLKRI
EEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERTYGVPGTIVLAIWGKESGFGRAKIPHPIMDVLATKAFMSTRPELFRRELIAALHILDS
HHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
GDVKEAEMRGSWAGAMGQPQFLPTSFLKYAVDFDGDGRRDIWNSVPDSLASIASYLAQKG
CCCCHHHHCCCCCCCCCCCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
WQRGRDWGFEVAIPSGVSCAQEGPDLARPIAEWAGMGIGRVSGKPFPAAERGASGMMLVP
HHCCCCCCEEEECCCCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEE
AGTHGPQFIVTPNFYVIKEYNNSDLYALYIGNLADRIATGGGAFRGKWGDVGGMLRSDVL
CCCCCCEEEECCCEEEEEEECCCCEEEEEECHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
GLQKALVAKGYDVGKADGLPGYKTRRSLGDWQAKNGLKPTCFPDVSLKAKLR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCCEECCC
>Mature Secondary Structure
MLRFLSVLFLASLFFVSASFAASRSDVEAQFRQWLQSDLWPEAQKAGVSSGAFKAAFKGV
CHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHCCC
RLNWDLPDLAPPGFPKPKQRKQSQAEFSSPGSYFSEKRLQGLAATGRSLASTHASTLKRI
EEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERTYGVPGTIVLAIWGKESGFGRAKIPHPIMDVLATKAFMSTRPELFRRELIAALHILDS
HHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
GDVKEAEMRGSWAGAMGQPQFLPTSFLKYAVDFDGDGRRDIWNSVPDSLASIASYLAQKG
CCCCHHHHCCCCCCCCCCCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
WQRGRDWGFEVAIPSGVSCAQEGPDLARPIAEWAGMGIGRVSGKPFPAAERGASGMMLVP
HHCCCCCCEEEECCCCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEE
AGTHGPQFIVTPNFYVIKEYNNSDLYALYIGNLADRIATGGGAFRGKWGDVGGMLRSDVL
CCCCCCEEEECCCEEEEEEECCCCEEEEEECHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
GLQKALVAKGYDVGKADGLPGYKTRRSLGDWQAKNGLKPTCFPDVSLKAKLR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]