Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 150398564

GI number: 150398564

Start: 3576850

End: 3577560

Strand: Direct

Name: nth [H]

Synonym: Smed_3375

Alternate gene names: 150398564

Gene position: 3576850-3577560 (Clockwise)

Preceding gene: 150398561

Following gene: 150398566

Centisome position: 94.58

GC content: 64.28

Gene sequence:

>711_bases
ATGAGCGCGCGGCAGCGCAGTGCCTACAGCACCGAGGAAGTGGAGGAGATCTTTCGCCGCTTTTCCGTTCAGCGCCCGGA
GCCGAAGGGCGAACTCGAACATGTCAATCCGTTCACACTGGTGGTGGCGGTCGCGCTTTCGGCGCAGGCGACCGACGCAG
GCGTCAACAAGGCGACGCGGGCGCTCTTCGCCGTCGCGGATACGCCCGAGAAGATGCTGGCGCTCGGGGAGGAGCGCGTT
CGCGACTATATCAAGACGATCGGTCTCTACCGCAACAAGGCGAAAAACGTCATTGCCCTGTCCCGCAAGCTGATCACCGA
CTTCGGGGGCGAGGTGCCGCGCACGCGCGAAGAGCTCGTGACGCTGCCGGGCGTCGGGCGAAAGACAGCGAATGTGGTGC
TCTCGATGGCCTTCGGCGAGGCGACCATCGCGGTCGACACCCATATCTTCCGTATCGCCCACCGTATCCGCATTGCACCC
GGCAAGACACCGGATGAGGTAGAGGCGCATCTTCTGCGGGTCATCCCCGAACACCGTCTGTACCACGCTCATCATTGGCT
GATCCTGCACGGCCGATACGTGTGCAAGGCGCGCCGGCCGGAATGCGAGCGCTGCGTCATTGCCGACATCTGCAAGTCGC
CGGAGAAGACCTCGGATATCCCGGCGCCGCTCGTCGAATTGCCGCCGCAGGCAATTGCCGCCGTCGGCTGA

Upstream 100 bases:

>100_bases
TAGATGCAGCATGAAAAACGTGAAGCTCAAATCGCCATCACAGCAGCCAAAGCCGGCGAAAGCGTCAACGGGACGCATGG
CGGGCCGCACGGGTGGCCGC

Downstream 100 bases:

>100_bases
TAGAACCCGAAGAAGGAGAGCGGGCGACAGGTTTCCCGATCCCGCTCGGCGCCATCGTCGTTACGGGGTGATCAGGCGAT
CGATGAGCCGGTCGATGGCG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MSARQRSAYSTEEVEEIFRRFSVQRPEPKGELEHVNPFTLVVAVALSAQATDAGVNKATRALFAVADTPEKMLALGEERV
RDYIKTIGLYRNKAKNVIALSRKLITDFGGEVPRTREELVTLPGVGRKTANVVLSMAFGEATIAVDTHIFRIAHRIRIAP
GKTPDEVEAHLLRVIPEHRLYHAHHWLILHGRYVCKARRPECERCVIADICKSPEKTSDIPAPLVELPPQAIAAVG

Sequences:

>Translated_236_residues
MSARQRSAYSTEEVEEIFRRFSVQRPEPKGELEHVNPFTLVVAVALSAQATDAGVNKATRALFAVADTPEKMLALGEERV
RDYIKTIGLYRNKAKNVIALSRKLITDFGGEVPRTREELVTLPGVGRKTANVVLSMAFGEATIAVDTHIFRIAHRIRIAP
GKTPDEVEAHLLRVIPEHRLYHAHHWLILHGRYVCKARRPECERCVIADICKSPEKTSDIPAPLVELPPQAIAAVG
>Mature_235_residues
SARQRSAYSTEEVEEIFRRFSVQRPEPKGELEHVNPFTLVVAVALSAQATDAGVNKATRALFAVADTPEKMLALGEERVR
DYIKTIGLYRNKAKNVIALSRKLITDFGGEVPRTREELVTLPGVGRKTANVVLSMAFGEATIAVDTHIFRIAHRIRIAPG
KTPDEVEAHLLRVIPEHRLYHAHHWLILHGRYVCKARRPECERCVIADICKSPEKTSDIPAPLVELPPQAIAAVG

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=94, Evalue=8e-20,
Organism=Homo sapiens, GI115298650, Length=146, Percent_Identity=31.5068493150685, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI190358497, Length=146, Percent_Identity=31.5068493150685, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI6912520, Length=146, Percent_Identity=31.5068493150685, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI115298648, Length=217, Percent_Identity=27.1889400921659, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI115298654, Length=146, Percent_Identity=31.5068493150685, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI115298652, Length=146, Percent_Identity=31.5068493150685, Blast_Score=71, Evalue=9e-13,
Organism=Escherichia coli, GI1787920, Length=201, Percent_Identity=56.2189054726368, Blast_Score=229, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI17554540, Length=180, Percent_Identity=31.6666666666667, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=198, Percent_Identity=25.7575757575758, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319304, Length=184, Percent_Identity=26.6304347826087, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550361, Length=179, Percent_Identity=29.0502793296089, Blast_Score=94, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26253; Mature: 26122

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARQRSAYSTEEVEEIFRRFSVQRPEPKGELEHVNPFTLVVAVALSAQATDAGVNKATR
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHH
ALFAVADTPEKMLALGEERVRDYIKTIGLYRNKAKNVIALSRKLITDFGGEVPRTREELV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHC
TLPGVGRKTANVVLSMAFGEATIAVDTHIFRIAHRIRIAPGKTPDEVEAHLLRVIPEHRL
CCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHH
YHAHHWLILHGRYVCKARRPECERCVIADICKSPEKTSDIPAPLVELPPQAIAAVG
HHHHEEEEEECCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHCCC
>Mature Secondary Structure 
SARQRSAYSTEEVEEIFRRFSVQRPEPKGELEHVNPFTLVVAVALSAQATDAGVNKATR
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHH
ALFAVADTPEKMLALGEERVRDYIKTIGLYRNKAKNVIALSRKLITDFGGEVPRTREELV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHC
TLPGVGRKTANVVLSMAFGEATIAVDTHIFRIAHRIRIAPGKTPDEVEAHLLRVIPEHRL
CCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHH
YHAHHWLILHGRYVCKARRPECERCVIADICKSPEKTSDIPAPLVELPPQAIAAVG
HHHHEEEEEECCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA