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The map label for this gene is purU [H]

Identifier: 150398500

GI number: 150398500

Start: 3503641

End: 3504525

Strand: Direct

Name: purU [H]

Synonym: Smed_3310

Alternate gene names: 150398500

Gene position: 3503641-3504525 (Clockwise)

Preceding gene: 150398499

Following gene: 150398501

Centisome position: 92.64

GC content: 60.23

Gene sequence:

>885_bases
ATGCAGAATTTCGTTCTGACTGTCACCTGCAAATCGACGCGCGGCATCGTCGCCGCCATCTCCGGATACCTGGCCGGGCA
GGGCTGCAACATCGTCGATTCGTCGCAATTCGATGACCTCGAAACGGGTCTGTTCTTCATGCGTATTAGCTTCATCTCCG
AGGAAAGGCTGGGCCTTGCGGCTCTTGAGGAGGGGCTGAAGCCGATTGCCTCGAAGTTCGAAATGGTGACGGCTCTTCAC
GAACAGTCGGAGCGGATGAAGGTTCTTCTGATGGTGTCGCGTTTCGGCCATTGCCTGAACGACCTGCTCTACCGCTGGAA
GATCGGGGCGCTGCCGATCGACATCGTCGGCGTCGTTTCCAACCATTTCGAGTACCAGAAGGTGGTCGTCAACCACGACA
TCCCCTTCCATTGCATCAAGGTGACCAAGGAGAACAAGCCCAGGGCCGAAGCCCAGCTGATGGAAGTGGTCGAGCAGACC
GGCGCCGAGCTGATCGTGCTTGCCCGCTACATGCAGGTGCTTTCGGATGCGCTGTGCAAGAAGATGTCGGGGAAGATCAT
CAACATCCACCACTCCTTCCTGCCGTCGTTCAAGGGGGCGAACCCCTACAAGCAGGCCTATGAGCGCGGCGTAAAGCTGA
TCGGCGCGACGGCGCATTACGTCACCGCCGATCTCGACGAGGGTCCGATCATCGAGCAGGACATCGCCCGCATCACCCAT
GCGCAGTCGGCCGAGGATTACGTCTCGATCGGCCGGGATGTCGAAAGCCAGGTGCTGGCGCGGGCCGTGCACGCCCATAT
CCACCACCGCTGCTTCATCAACGGCAACCGCGTCGTCGTCTTCCCGCCGAGCCCGGGAAGCTACGCCTCCGAACGGATGG
GCTGA

Upstream 100 bases:

>100_bases
CTGCTCTGCTAGCAATTGCACCATCGGCCGGGATGGGGATCGACGTGCGCCGGGACGCCCCATGGACCCGCGCGGGTTGA
ATGAGAGGAGAGCAATGCGC

Downstream 100 bases:

>100_bases
GGCTGGTTCAGGTCGCGGCCCGCCGGATCGGCTGTCTCGTGGCGCCCGTCGCCCCGAGCTCGAAGCCGTCGAAACCGTTG
AGAAGCGGTGCCGCGCCGGG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MQNFVLTVTCKSTRGIVAAISGYLAGQGCNIVDSSQFDDLETGLFFMRISFISEERLGLAALEEGLKPIASKFEMVTALH
EQSERMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFEYQKVVVNHDIPFHCIKVTKENKPRAEAQLMEVVEQT
GAELIVLARYMQVLSDALCKKMSGKIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTADLDEGPIIEQDIARITH
AQSAEDYVSIGRDVESQVLARAVHAHIHHRCFINGNRVVVFPPSPGSYASERMG

Sequences:

>Translated_294_residues
MQNFVLTVTCKSTRGIVAAISGYLAGQGCNIVDSSQFDDLETGLFFMRISFISEERLGLAALEEGLKPIASKFEMVTALH
EQSERMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFEYQKVVVNHDIPFHCIKVTKENKPRAEAQLMEVVEQT
GAELIVLARYMQVLSDALCKKMSGKIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTADLDEGPIIEQDIARITH
AQSAEDYVSIGRDVESQVLARAVHAHIHHRCFINGNRVVVFPPSPGSYASERMG
>Mature_294_residues
MQNFVLTVTCKSTRGIVAAISGYLAGQGCNIVDSSQFDDLETGLFFMRISFISEERLGLAALEEGLKPIASKFEMVTALH
EQSERMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFEYQKVVVNHDIPFHCIKVTKENKPRAEAQLMEVVEQT
GAELIVLARYMQVLSDALCKKMSGKIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTADLDEGPIIEQDIARITH
AQSAEDYVSIGRDVESQVLARAVHAHIHHRCFINGNRVVVFPPSPGSYASERMG

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=106, Percent_Identity=37.7358490566038, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI209869995, Length=106, Percent_Identity=37.7358490566038, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI209869993, Length=106, Percent_Identity=37.7358490566038, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1787483, Length=283, Percent_Identity=39.5759717314488, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1788846, Length=168, Percent_Identity=25.5952380952381, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=154, Percent_Identity=33.7662337662338, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32713; Mature: 32713

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNFVLTVTCKSTRGIVAAISGYLAGQGCNIVDSSQFDDLETGLFFMRISFISEERLGLA
CCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHH
ALEEGLKPIASKFEMVTALHEQSERMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
NHFEYQKVVVNHDIPFHCIKVTKENKPRAEAQLMEVVEQTGAELIVLARYMQVLSDALCK
CCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KMSGKIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTADLDEGPIIEQDIARITH
HHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEEECCCCCCHHHHHHHHHH
AQSAEDYVSIGRDVESQVLARAVHAHIHHRCFINGNRVVVFPPSPGSYASERMG
HCCHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEECCEEEEECCCCCCCHHHCCC
>Mature Secondary Structure
MQNFVLTVTCKSTRGIVAAISGYLAGQGCNIVDSSQFDDLETGLFFMRISFISEERLGLA
CCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHH
ALEEGLKPIASKFEMVTALHEQSERMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
NHFEYQKVVVNHDIPFHCIKVTKENKPRAEAQLMEVVEQTGAELIVLARYMQVLSDALCK
CCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KMSGKIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTADLDEGPIIEQDIARITH
HHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEEECCCCCCHHHHHHHHHH
AQSAEDYVSIGRDVESQVLARAVHAHIHHRCFINGNRVVVFPPSPGSYASERMG
HCCHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEECCEEEEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]