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The map label for this gene is alkB [H]

Identifier: 150398448

GI number: 150398448

Start: 3437367

End: 3437978

Strand: Direct

Name: alkB [H]

Synonym: Smed_3256

Alternate gene names: 150398448

Gene position: 3437367-3437978 (Clockwise)

Preceding gene: 150398447

Following gene: 150398456

Centisome position: 90.89

GC content: 66.01

Gene sequence:

>612_bases
ATGCTGGTGCTGCCGAAAGGAGTGAGACACATTCCGGGTTTCCTAGACCGCTCCCGCCAGGAGGAACTGGTCGAGGCGAT
CCGAATCGTCGTCGCGGAGGCGCCGCTCTTCGCACCGGCGATGCCGAAAACCGGCAAACCCCTGTCCGTGCGCATGACCA
ATTGCGGGGCGCTCGGCTGGGTCACCGACCGCGAGAACGGCTACCGCTACCAAGCGGTGCATCCGGTCACCGGCAGGCCC
TGGCCGCCGATACCGGCGGCGCTGCAGGACATCTGGAATGCCGTAGCCGGCAGCGACAAGACGCCCGAGGCCTGTCTGGT
GAATTTCTATTCTGCGGAAGCGCGCATGGGGCTCCATCAGGACCGGGACGAGCGCGATCTTGACACCGCCGTCGTTTCGG
TCTCGCTCGGCGACAGCTGTCTCTTCCGCGTCGGCGGCCGTACGCGCGGCGGGCCGACCGTTTCGCTCAAGCTCGAAAGC
GGCGATGTCGTCGTGCTCGGCGGTGAAGGACGTCTCGCCTTCCACGGCGTCGACCGCATCTATCCGAACACCTCGACACT
CCTGAGGAGCGGCGGCCGGCTCAATCTGACCTTGCGGCGCGTCAATCCTTGA

Upstream 100 bases:

>100_bases
CGGGTCGTTTCCAGCGGGGGTGAGCGCTACATCTCGTCACGTCCAGTCAGCTTTGCTGACGTAAGGACTTGATCGACATC
GCAAGACAGGACGCTCGCAA

Downstream 100 bases:

>100_bases
ACCCTTGAACCGTCCAAAGCATATCGCCTCTCCCCCGCCTGTCGGGAAAAGGTGCGGCAGCCGCCTGAGGGCCGAAATTC
GGCTCGGATCATGATTTTTT

Product: 2OG-Fe(II) oxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MLVLPKGVRHIPGFLDRSRQEELVEAIRIVVAEAPLFAPAMPKTGKPLSVRMTNCGALGWVTDRENGYRYQAVHPVTGRP
WPPIPAALQDIWNAVAGSDKTPEACLVNFYSAEARMGLHQDRDERDLDTAVVSVSLGDSCLFRVGGRTRGGPTVSLKLES
GDVVVLGGEGRLAFHGVDRIYPNTSTLLRSGGRLNLTLRRVNP

Sequences:

>Translated_203_residues
MLVLPKGVRHIPGFLDRSRQEELVEAIRIVVAEAPLFAPAMPKTGKPLSVRMTNCGALGWVTDRENGYRYQAVHPVTGRP
WPPIPAALQDIWNAVAGSDKTPEACLVNFYSAEARMGLHQDRDERDLDTAVVSVSLGDSCLFRVGGRTRGGPTVSLKLES
GDVVVLGGEGRLAFHGVDRIYPNTSTLLRSGGRLNLTLRRVNP
>Mature_203_residues
MLVLPKGVRHIPGFLDRSRQEELVEAIRIVVAEAPLFAPAMPKTGKPLSVRMTNCGALGWVTDRENGYRYQAVHPVTGRP
WPPIPAALQDIWNAVAGSDKTPEACLVNFYSAEARMGLHQDRDERDLDTAVVSVSLGDSCLFRVGGRTRGGPTVSLKLES
GDVVVLGGEGRLAFHGVDRIYPNTSTLLRSGGRLNLTLRRVNP

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=131, Percent_Identity=32.0610687022901, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1788541, Length=185, Percent_Identity=41.6216216216216, Blast_Score=139, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45555401, Length=87, Percent_Identity=35.632183908046, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 22094; Mature: 22094

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLPKGVRHIPGFLDRSRQEELVEAIRIVVAEAPLFAPAMPKTGKPLSVRMTNCGALGW
CEECCCCHHHCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEE
VTDRENGYRYQAVHPVTGRPWPPIPAALQDIWNAVAGSDKTPEACLVNFYSAEARMGLHQ
EEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHEEEEEECHHHHCCCCC
DRDERDLDTAVVSVSLGDSCLFRVGGRTRGGPTVSLKLESGDVVVLGGEGRLAFHGVDRI
CCCCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEEECCCCEEEECCCCEEEEECCHHC
YPNTSTLLRSGGRLNLTLRRVNP
CCCHHHHHHCCCEEEEEEEECCC
>Mature Secondary Structure
MLVLPKGVRHIPGFLDRSRQEELVEAIRIVVAEAPLFAPAMPKTGKPLSVRMTNCGALGW
CEECCCCHHHCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEE
VTDRENGYRYQAVHPVTGRPWPPIPAALQDIWNAVAGSDKTPEACLVNFYSAEARMGLHQ
EEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHEEEEEECHHHHCCCCC
DRDERDLDTAVVSVSLGDSCLFRVGGRTRGGPTVSLKLESGDVVVLGGEGRLAFHGVDRI
CCCCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEEECCCCEEEECCCCEEEEECCHHC
YPNTSTLLRSGGRLNLTLRRVNP
CCCHHHHHHCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9150207 [H]