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The map label for this gene is paaF [C]

Identifier: 150396087

GI number: 150396087

Start: 922502

End: 923323

Strand: Direct

Name: paaF [C]

Synonym: Smed_0864

Alternate gene names: 150396087

Gene position: 922502-923323 (Clockwise)

Preceding gene: 150396081

Following gene: 150396089

Centisome position: 24.39

GC content: 61.19

Gene sequence:

>822_bases
ATGGCGGATGTCGTTTCATTCAGGAAAGAAGAACAGAGCGGACCGTTGCTACGTGAGGTCAACAGCCCGGTCCTGCGTTT
GACGCTCAACAATCCGCCTGCCAACGTGCTTTCGATCGCCTTGATGGAAGCCTTGCTGGCGGCGCTCGAGGAGGCGGCCG
CCTCGAAGGAAATCAAGGTTGTCGTGATCGCCGCGGCCGGCAAGCTTTTCTCCGCCGGCCACGATCTCAAGGAAATGACC
TCCCATCGGGCCCACGCGGACGGCGGACGGGCTTTCTTCGAGCGAACGATGCGCCTGGCGGCCGAAGTGATGCTGACCAT
TACCAGGCTGCCGCAGGCGGTCATTGCAGAAGTCGACGGTCTCGCGACGGCCGCTGGGTGCCAGCTTGTCGCAAGTTGCG
ACCTGGCGATCTGCACCGACACGTCCACCTTCTGCACCCCGGGTGTGAACATTGGCCTTTTCTGTTCGACGCCGATGGTC
GCTCTGACACGCGCGGCCCATCGCAAGCAGGCGATGGAAATGCTCCTGACCGGTGAGCCGATAGATGCCGGGACAGCGAA
GGAGTTCGGTCTCGTCAACCGCATCGTTCCTCAGCAATATCTGCGCCAGGTCATCGACAAATATGCGTCGGTCATTGCCA
CGAAGTCGCCGCAGGCACTGAAGATCGGCAAGGAAGCCTTCTACCGGCAGGCGGAAATGCCGCTTGCCGAGGCTTACGAC
TTTGCAGTCGGCGTCATGGTCGAGAACATGCTCGCCCGTGATGCCGAGGAGGGCATCGGCGCCTTTCTCGGCAAGCGCAT
GCCGGATTGGAAGGAAGACTAG

Upstream 100 bases:

>100_bases
CCGGAAATTGGTCTTAGCTCGATTTCGCCGGTCCGGCGCGGGGTTGAAAGAGCGGCGACGGCGGCGTACCACGGCAGCAC
AGTCGATATGGGAGAGAACC

Downstream 100 bases:

>100_bases
GGCAGAATGAGGAGAACTGCGCCCGGTTTCCCGCCCGCATCCCGCTCTGACCTTTAGAATGGATCACGCGAGCCTGAGGA
GCAAAGCGCCGAGCGCAATG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MADVVSFRKEEQSGPLLREVNSPVLRLTLNNPPANVLSIALMEALLAALEEAAASKEIKVVVIAAAGKLFSAGHDLKEMT
SHRAHADGGRAFFERTMRLAAEVMLTITRLPQAVIAEVDGLATAAGCQLVASCDLAICTDTSTFCTPGVNIGLFCSTPMV
ALTRAAHRKQAMEMLLTGEPIDAGTAKEFGLVNRIVPQQYLRQVIDKYASVIATKSPQALKIGKEAFYRQAEMPLAEAYD
FAVGVMVENMLARDAEEGIGAFLGKRMPDWKED

Sequences:

>Translated_273_residues
MADVVSFRKEEQSGPLLREVNSPVLRLTLNNPPANVLSIALMEALLAALEEAAASKEIKVVVIAAAGKLFSAGHDLKEMT
SHRAHADGGRAFFERTMRLAAEVMLTITRLPQAVIAEVDGLATAAGCQLVASCDLAICTDTSTFCTPGVNIGLFCSTPMV
ALTRAAHRKQAMEMLLTGEPIDAGTAKEFGLVNRIVPQQYLRQVIDKYASVIATKSPQALKIGKEAFYRQAEMPLAEAYD
FAVGVMVENMLARDAEEGIGAFLGKRMPDWKED
>Mature_272_residues
ADVVSFRKEEQSGPLLREVNSPVLRLTLNNPPANVLSIALMEALLAALEEAAASKEIKVVVIAAAGKLFSAGHDLKEMTS
HRAHADGGRAFFERTMRLAAEVMLTITRLPQAVIAEVDGLATAAGCQLVASCDLAICTDTSTFCTPGVNIGLFCSTPMVA
LTRAAHRKQAMEMLLTGEPIDAGTAKEFGLVNRIVPQQYLRQVIDKYASVIATKSPQALKIGKEAFYRQAEMPLAEAYDF
AVGVMVENMLARDAEEGIGAFLGKRMPDWKED

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=267, Percent_Identity=44.1947565543071, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=30.7392996108949, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI70995211, Length=227, Percent_Identity=25.5506607929515, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=33.59375, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=30.5343511450382, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI87082183, Length=243, Percent_Identity=28.8065843621399, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=25.3968253968254, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=26.7441860465116, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=251, Percent_Identity=41.0358565737052, Blast_Score=186, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=31.6, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=30.1204819277108, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=250, Percent_Identity=26.8, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=233, Percent_Identity=25.3218884120172, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=227, Percent_Identity=28.1938325991189, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19922422, Length=272, Percent_Identity=38.9705882352941, Blast_Score=183, Evalue=8e-47,
Organism=Drosophila melanogaster, GI20129971, Length=242, Percent_Identity=31.404958677686, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24653477, Length=242, Percent_Identity=31.404958677686, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24654903, Length=220, Percent_Identity=28.6363636363636, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920382, Length=260, Percent_Identity=26.5384615384615, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29425; Mature: 29293

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVVSFRKEEQSGPLLREVNSPVLRLTLNNPPANVLSIALMEALLAALEEAAASKEIKV
CCCHHHHHHHHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
VVIAAAGKLFSAGHDLKEMTSHRAHADGGRAFFERTMRLAAEVMLTITRLPQAVIAEVDG
EEEEECCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LATAAGCQLVASCDLAICTDTSTFCTPGVNIGLFCSTPMVALTRAAHRKQAMEMLLTGEP
HHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCC
IDAGTAKEFGLVNRIVPQQYLRQVIDKYASVIATKSPQALKIGKEAFYRQAEMPLAEAYD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHH
FAVGVMVENMLARDAEEGIGAFLGKRMPDWKED
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ADVVSFRKEEQSGPLLREVNSPVLRLTLNNPPANVLSIALMEALLAALEEAAASKEIKV
CCHHHHHHHHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
VVIAAAGKLFSAGHDLKEMTSHRAHADGGRAFFERTMRLAAEVMLTITRLPQAVIAEVDG
EEEEECCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LATAAGCQLVASCDLAICTDTSTFCTPGVNIGLFCSTPMVALTRAAHRKQAMEMLLTGEP
HHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCC
IDAGTAKEFGLVNRIVPQQYLRQVIDKYASVIATKSPQALKIGKEAFYRQAEMPLAEAYD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHH
FAVGVMVENMLARDAEEGIGAFLGKRMPDWKED
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA