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The map label for this gene is smeIM [H]

Identifier: 150395765

GI number: 150395765

Start: 584422

End: 585618

Strand: Direct

Name: smeIM [H]

Synonym: Smed_0541

Alternate gene names: 150395765

Gene position: 584422-585618 (Clockwise)

Preceding gene: 150395761

Following gene: 150395768

Centisome position: 15.45

GC content: 60.74

Gene sequence:

>1197_bases
TTGGTAACCAACTTCGGTAACCATACGCCCATAGACAGTCCGAGTAAGCGTATTTGCGAGTTGCCAATGTCCTCAGTTGT
TTCGCTTGCCGAAATCTCCCGTGCCGCCCGTCCTTTGAACTGGCTCGACAGCATCATCAAGGGGGATTGCGTGGCCGCGC
TGAATGCTCTTCCCGACAATTCGGTCGATGTCGTCTTCGCCGACCCGCCCTATAATCTCCAACTCGGCGGCACGCTGCAC
CGGCCTGATCAGTCGCTGGTCGATGCGGTGGACGATGACTGGGACCAGTTTGCGTCCTTCGAAGCCTATGACGCCTTTAC
CCGCGCCTGGCTGCTCGCCTGCCGCCGCGTGCTGAAGCCCACCGGCACGCTCTGGGTCATCGGTTCCTACCACAATATCT
TCCGGGTCGGCGCAATCCTTCAGGACCTGCATTTCTGGGTTCTGAACGATATCATCTGGCGCAAGACCAACCCGATGCCG
AACTTCAAGGGGCGCCGCTTCCAGAACGCTCATGAAACGCTGATCTGGGCGACGCCGAACGCCAAGGCAAAGGGGTACAC
CTTCAATTATGAGGCGATGAAGGCGGCGAATGACGACGTTCAGATGCGTTCCGACTGGCTGTTCCCGATCTGCTCCGGTT
CGGAGCGGCTGAAGGGAGACGACGGCAAGAAGGTGCACCCGACGCAAAAGCCGGAAGCGCTGCTTGCCCGCATTCTGATG
GCCTCGACCAAACCCGGCGACGTTGTGCTCGATCCTTTCTTCGGCTCCGGCACCACCGGGGCAGTCGCAAAGCGCCTCGG
CCGGCACTTTGTCGGCATCGAGCGCGAACAGGACTATATCGATGCAGCCGCCGAACGCATCGCGGCTGTCGAGCCTCTCG
GCAAAGCGACGCTTTCCGTCATGACCGGCAAGAAAGCCGAGCCGCGTGTCGCCTTCAACACGCTCATCGAAAGCGGGCTC
ATCAAGCCCGGCACGGTTCTGACGGACGCGAAGCGCCGCTACAGCGCGATCGTTCGCGCCGACGGCACGCTGGCGTCCGG
TGGCGAGGCTGGCTCCATTCACCGCCTTGGCGCGAAAGTGCAAGGCCTCGACGCCTGCAATGGCTGGACCTTCTGGCACT
TCGAAGACGGAAGCGTGTTGAAGCCGATCGACGAGCTCAGATCCGTCATTCGAAACGACCTGGCAAAACTGAACTGA

Upstream 100 bases:

>100_bases
TTCATACTTCGATGTTAGGCGAAGTATGACTGCAGGGCAAGCGGCACGAACGGCAGAGCGCCGCTGCGGCGGCGCAATTT
TTTCCATCCTCCTTCAGGCT

Downstream 100 bases:

>100_bases
TCAACCAGTTCCGCCGCGGTCTTCGATAGGCGCCGCCTCCGGTTTTCGTACCTTCAGTCCCGGAAAGCGCGCTTTAAACG
CCCGGAATCCGAAGAGGATT

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MVTNFGNHTPIDSPSKRICELPMSSVVSLAEISRAARPLNWLDSIIKGDCVAALNALPDNSVDVVFADPPYNLQLGGTLH
RPDQSLVDAVDDDWDQFASFEAYDAFTRAWLLACRRVLKPTGTLWVIGSYHNIFRVGAILQDLHFWVLNDIIWRKTNPMP
NFKGRRFQNAHETLIWATPNAKAKGYTFNYEAMKAANDDVQMRSDWLFPICSGSERLKGDDGKKVHPTQKPEALLARILM
ASTKPGDVVLDPFFGSGTTGAVAKRLGRHFVGIEREQDYIDAAAERIAAVEPLGKATLSVMTGKKAEPRVAFNTLIESGL
IKPGTVLTDAKRRYSAIVRADGTLASGGEAGSIHRLGAKVQGLDACNGWTFWHFEDGSVLKPIDELRSVIRNDLAKLN

Sequences:

>Translated_398_residues
MVTNFGNHTPIDSPSKRICELPMSSVVSLAEISRAARPLNWLDSIIKGDCVAALNALPDNSVDVVFADPPYNLQLGGTLH
RPDQSLVDAVDDDWDQFASFEAYDAFTRAWLLACRRVLKPTGTLWVIGSYHNIFRVGAILQDLHFWVLNDIIWRKTNPMP
NFKGRRFQNAHETLIWATPNAKAKGYTFNYEAMKAANDDVQMRSDWLFPICSGSERLKGDDGKKVHPTQKPEALLARILM
ASTKPGDVVLDPFFGSGTTGAVAKRLGRHFVGIEREQDYIDAAAERIAAVEPLGKATLSVMTGKKAEPRVAFNTLIESGL
IKPGTVLTDAKRRYSAIVRADGTLASGGEAGSIHRLGAKVQGLDACNGWTFWHFEDGSVLKPIDELRSVIRNDLAKLN
>Mature_398_residues
MVTNFGNHTPIDSPSKRICELPMSSVVSLAEISRAARPLNWLDSIIKGDCVAALNALPDNSVDVVFADPPYNLQLGGTLH
RPDQSLVDAVDDDWDQFASFEAYDAFTRAWLLACRRVLKPTGTLWVIGSYHNIFRVGAILQDLHFWVLNDIIWRKTNPMP
NFKGRRFQNAHETLIWATPNAKAKGYTFNYEAMKAANDDVQMRSDWLFPICSGSERLKGDDGKKVHPTQKPEALLARILM
ASTKPGDVVLDPFFGSGTTGAVAKRLGRHFVGIEREQDYIDAAAERIAAVEPLGKATLSVMTGKKAEPRVAFNTLIESGL
IKPGTVLTDAKRRYSAIVRADGTLASGGEAGSIHRLGAKVQGLDACNGWTFWHFEDGSVLKPIDELRSVIRNDLAKLN

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=286, Percent_Identity=31.4685314685315, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 43858; Mature: 43858

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTNFGNHTPIDSPSKRICELPMSSVVSLAEISRAARPLNWLDSIIKGDCVAALNALPDN
CCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCC
SVDVVFADPPYNLQLGGTLHRPDQSLVDAVDDDWDQFASFEAYDAFTRAWLLACRRVLKP
CEEEEECCCCCCEEECCEECCCCHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCC
TGTLWVIGSYHNIFRVGAILQDLHFWVLNDIIWRKTNPMPNFKGRRFQNAHETLIWATPN
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCC
AKAKGYTFNYEAMKAANDDVQMRSDWLFPICSGSERLKGDDGKKVHPTQKPEALLARILM
CCCCCEEECHHHHHCCCCCCEECCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ASTKPGDVVLDPFFGSGTTGAVAKRLGRHFVGIEREQDYIDAAAERIAAVEPLGKATLSV
HCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
MTGKKAEPRVAFNTLIESGLIKPGTVLTDAKRRYSAIVRADGTLASGGEAGSIHRLGAKV
HCCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCC
QGLDACNGWTFWHFEDGSVLKPIDELRSVIRNDLAKLN
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVTNFGNHTPIDSPSKRICELPMSSVVSLAEISRAARPLNWLDSIIKGDCVAALNALPDN
CCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCC
SVDVVFADPPYNLQLGGTLHRPDQSLVDAVDDDWDQFASFEAYDAFTRAWLLACRRVLKP
CEEEEECCCCCCEEECCEECCCCHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCC
TGTLWVIGSYHNIFRVGAILQDLHFWVLNDIIWRKTNPMPNFKGRRFQNAHETLIWATPN
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCC
AKAKGYTFNYEAMKAANDDVQMRSDWLFPICSGSERLKGDDGKKVHPTQKPEALLARILM
CCCCCEEECHHHHHCCCCCCEECCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ASTKPGDVVLDPFFGSGTTGAVAKRLGRHFVGIEREQDYIDAAAERIAAVEPLGKATLSV
HCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
MTGKKAEPRVAFNTLIESGLIKPGTVLTDAKRRYSAIVRADGTLASGGEAGSIHRLGAKV
HCCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCC
QGLDACNGWTFWHFEDGSVLKPIDELRSVIRNDLAKLN
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]