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The map label for this gene is trmB [H]

Identifier: 150395275

GI number: 150395275

Start: 49601

End: 50299

Strand: Direct

Name: trmB [H]

Synonym: Smed_0047

Alternate gene names: 150395275

Gene position: 49601-50299 (Clockwise)

Preceding gene: 150395274

Following gene: 150395278

Centisome position: 1.31

GC content: 62.52

Gene sequence:

>699_bases
ATGACCGAAACGCGCGGTGCCAGAGCGACGGAGGCCTTCTTCGGTCGCCGGAAGGGCAAACCCCTGCGGGAACGCCAGGC
GGCACATCTCGAAAACCTGCTGCCTGAATTGAAGATCGACCTCGAACACGCGGCTCCGGCCGATTTGAAGGAACTCTTTC
CCGTGTCCGTCGAACGCGTTCGCCTGGAGATAGGTTTTGGCGGCGGCGAGCACCTGATCCATCGCGCCGCGGAAGATCCC
GCCACCGGCTTTATCGGCGTGGAACCCTTTGTCAATTCGATGGCGAAACTGCTCGGGCAGATCGAGGCGAAGGCTATTCG
CAACATCCGGCTCCATGACGACGACGCGACGCAGGTGCTGGATTGGCTGCCGGCGGCATCCGTCGACCAGATCGATCTGC
TTTATCCCGACCCCTGGCCGAAACGGAAGCACTGGAAGCGGCGTTTCGTCTCCGAGGTCAACCTCGACCGCTTCGCACGC
ATCCTGAGGCCCGGCGGTGTCTTCTGCTTCGCCTCGGACATCGACAGCTATATCAATTGGACGCTCATCCATTGCCGTGA
CCACGCGGCCTTCGAATGGACGGCGGCAGGAGCGGCGGACTGGCTGGCACCCTTTGCCGGATGGCCAAGCACCCGCTACG
AAGCGAAGGCGCGCAGGGAAGGGCGCAGTTCGGCCTATTTGAGCTTTCGGCGCAGTTGA

Upstream 100 bases:

>100_bases
CTTCCGGTAAAACACCGTAACGCAGCACCCTTTTGAAACGACGCACTTCCGAACGAAGGATGGTTCACCCTGTTCCGGTA
AGTGCCCAGGGAAGAACGAC

Downstream 100 bases:

>100_bases
GCTTGGCCACTGGAGTTTGGCCCCTCATCCGGTCTGGCGGCCACTTTCACCCCGCAGGAGGGGCGAAGGGCACGTGCGTT
GCCATCGGAACCCGAGCGGG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MTETRGARATEAFFGRRKGKPLRERQAAHLENLLPELKIDLEHAAPADLKELFPVSVERVRLEIGFGGGEHLIHRAAEDP
ATGFIGVEPFVNSMAKLLGQIEAKAIRNIRLHDDDATQVLDWLPAASVDQIDLLYPDPWPKRKHWKRRFVSEVNLDRFAR
ILRPGGVFCFASDIDSYINWTLIHCRDHAAFEWTAAGAADWLAPFAGWPSTRYEAKARREGRSSAYLSFRRS

Sequences:

>Translated_232_residues
MTETRGARATEAFFGRRKGKPLRERQAAHLENLLPELKIDLEHAAPADLKELFPVSVERVRLEIGFGGGEHLIHRAAEDP
ATGFIGVEPFVNSMAKLLGQIEAKAIRNIRLHDDDATQVLDWLPAASVDQIDLLYPDPWPKRKHWKRRFVSEVNLDRFAR
ILRPGGVFCFASDIDSYINWTLIHCRDHAAFEWTAAGAADWLAPFAGWPSTRYEAKARREGRSSAYLSFRRS
>Mature_231_residues
TETRGARATEAFFGRRKGKPLRERQAAHLENLLPELKIDLEHAAPADLKELFPVSVERVRLEIGFGGGEHLIHRAAEDPA
TGFIGVEPFVNSMAKLLGQIEAKAIRNIRLHDDDATQVLDWLPAASVDQIDLLYPDPWPKRKHWKRRFVSEVNLDRFARI
LRPGGVFCFASDIDSYINWTLIHCRDHAAFEWTAAGAADWLAPFAGWPSTRYEAKARREGRSSAYLSFRRS

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=219, Percent_Identity=30.1369863013699, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26344; Mature: 26213

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETRGARATEAFFGRRKGKPLRERQAAHLENLLPELKIDLEHAAPADLKELFPVSVERV
CCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHCCCCEEEE
RLEIGFGGGEHLIHRAAEDPATGFIGVEPFVNSMAKLLGQIEAKAIRNIRLHDDDATQVL
EEEEECCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
DWLPAASVDQIDLLYPDPWPKRKHWKRRFVSEVNLDRFARILRPGGVFCFASDIDSYINW
HHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCCHHHHHCE
TLIHCRDHAAFEWTAAGAADWLAPFAGWPSTRYEAKARREGRSSAYLSFRRS
EEEEECCCCCEEEECCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
TETRGARATEAFFGRRKGKPLRERQAAHLENLLPELKIDLEHAAPADLKELFPVSVERV
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHCCCCEEEE
RLEIGFGGGEHLIHRAAEDPATGFIGVEPFVNSMAKLLGQIEAKAIRNIRLHDDDATQVL
EEEEECCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
DWLPAASVDQIDLLYPDPWPKRKHWKRRFVSEVNLDRFARILRPGGVFCFASDIDSYINW
HHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCCHHHHHCE
TLIHCRDHAAFEWTAAGAADWLAPFAGWPSTRYEAKARREGRSSAYLSFRRS
EEEEECCCCCEEEECCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]