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The map label for this gene is metK [H]

Identifier: 150395274

GI number: 150395274

Start: 48104

End: 49384

Strand: Direct

Name: metK [H]

Synonym: Smed_0046

Alternate gene names: 150395274

Gene position: 48104-49384 (Clockwise)

Preceding gene: 150395273

Following gene: 150395275

Centisome position: 1.27

GC content: 60.89

Gene sequence:

>1281_bases
ATGCGCGCAAACTACCTGTTCACGAGTGAATCCGTAGCCGAAGGTCATCCGGATAAAGTGTGTGACCGTATCTCCGACGA
AATCGTCGATCTGGTCTACCGCGAAGCGGCGAAGACCGGTGTCGATCCTTGGACTGTGCGCATCGCCTGTGAAACGCTGG
CGACGACCAATCGCGTCGTCATCGCCGGGGAGGTCCGCCTGCCGCCGAGTCTTTTGAAGAAAGACAAGAACGGCAACGAT
GTCATCAATCCCTCGAAGTTCAAGTCTGCCGCCCGCAAGGCGATTCGCGATATCGGCTACGAGCAGGACGGCTTCCATTG
GAAGACGGCGAAGATAGACGTTCTTCTGCACTTCCAGTCGGCGCACATCGCACAGGGCGTGGACAACGCATCGGACAAGC
AGGGCGAGGAAGGTGCCGGCGACCAGGGCATCATGTTCGGCTATGCCTGCCGCGAGACCGCCGAGCTCATGCCGGCGCCG
ATCTACTATTCGCACCGCATTCTGAACCTGCTTGCCGCAGCCCGCAAAAAGGGCGAAGGCGAGGTCGCAAAGTTGGGTCC
GGACGCCAAGAGCCAGGTGACGGTACGCTACATAGACGGCAAGCCGGCCGAAGTCACCTCGATCGTTCTTTCCACGCAGC
ATCTCGACGATAGCTGGGATTCCGCCAAGGTTCGCTCCGTCGTTGAACCCTATATTCACGAAGCGCTGGGAGACCTGAAG
ATCGCCGACGACTGCACCTGGTACATCAATCCTACCGGCAAATTCGTCATCGGCGGCCCGGACGGCGATGCGGGTCTTAC
CGGTCGCAAGATCATCGTGGACACCTATGGCGGTGCTGCCCCGCATGGCGGCGGCGCATTCTCCGGCAAGGACACGACCA
AGGTCGATCGCTCGGCGGCCTATGCCGCACGCTATCTTGCCAAGAACGTCGTGGCGGCGGGTCTTGCCGAGCGTTGCACC
ATACAGCTTTCTTACGCAATCGGCGTCGCCCAGCCGCTGTCGATCTACGTCGACCTGCACGGCACCGGCAAGGTCTCGGA
AGATCAGGTGGAGAACGCAATCCGCAAGACGATGGACCTGTCGCCGACCGGCATCCGACGCCATCTCGACCTCAACCGGC
CGATCTATGCAAAGACCTCGGCCTACGGTCACTTCGGACGGAAGGCAGGCCGCGACGGGTCGTTCTCTTGGGAAAAGCTC
GACCTGGTCAAGCCGCTGAAAGAAGCAATCGAGAGCGACTCTTCGGCCGTCAACGGACGAGCGGCAAGCCGCGCAGCCTG
A

Upstream 100 bases:

>100_bases
ATGCCGATATAAAGATATATTTATGTCGTTGTGTGCTTGTTTTTCGCCGGCAAAACCACTAAACGTTTGACCGCTTCTTT
TCATTGAGGGGACTTCCCGC

Downstream 100 bases:

>100_bases
GGGCCGCGGGTGAGGCAGCTTCGGAAAGAGGGCCGTTGAAGATAGCGCAGCCGCCATTATCAGGCGCGGCATCGATCAGG
GCGGGCGCTCCGGGAGGAAC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MRANYLFTSESVAEGHPDKVCDRISDEIVDLVYREAAKTGVDPWTVRIACETLATTNRVVIAGEVRLPPSLLKKDKNGND
VINPSKFKSAARKAIRDIGYEQDGFHWKTAKIDVLLHFQSAHIAQGVDNASDKQGEEGAGDQGIMFGYACRETAELMPAP
IYYSHRILNLLAAARKKGEGEVAKLGPDAKSQVTVRYIDGKPAEVTSIVLSTQHLDDSWDSAKVRSVVEPYIHEALGDLK
IADDCTWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVVAAGLAERCT
IQLSYAIGVAQPLSIYVDLHGTGKVSEDQVENAIRKTMDLSPTGIRRHLDLNRPIYAKTSAYGHFGRKAGRDGSFSWEKL
DLVKPLKEAIESDSSAVNGRAASRAA

Sequences:

>Translated_426_residues
MRANYLFTSESVAEGHPDKVCDRISDEIVDLVYREAAKTGVDPWTVRIACETLATTNRVVIAGEVRLPPSLLKKDKNGND
VINPSKFKSAARKAIRDIGYEQDGFHWKTAKIDVLLHFQSAHIAQGVDNASDKQGEEGAGDQGIMFGYACRETAELMPAP
IYYSHRILNLLAAARKKGEGEVAKLGPDAKSQVTVRYIDGKPAEVTSIVLSTQHLDDSWDSAKVRSVVEPYIHEALGDLK
IADDCTWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVVAAGLAERCT
IQLSYAIGVAQPLSIYVDLHGTGKVSEDQVENAIRKTMDLSPTGIRRHLDLNRPIYAKTSAYGHFGRKAGRDGSFSWEKL
DLVKPLKEAIESDSSAVNGRAASRAA
>Mature_426_residues
MRANYLFTSESVAEGHPDKVCDRISDEIVDLVYREAAKTGVDPWTVRIACETLATTNRVVIAGEVRLPPSLLKKDKNGND
VINPSKFKSAARKAIRDIGYEQDGFHWKTAKIDVLLHFQSAHIAQGVDNASDKQGEEGAGDQGIMFGYACRETAELMPAP
IYYSHRILNLLAAARKKGEGEVAKLGPDAKSQVTVRYIDGKPAEVTSIVLSTQHLDDSWDSAKVRSVVEPYIHEALGDLK
IADDCTWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVVAAGLAERCT
IQLSYAIGVAQPLSIYVDLHGTGKVSEDQVENAIRKTMDLSPTGIRRHLDLNRPIYAKTSAYGHFGRKAGRDGSFSWEKL
DLVKPLKEAIESDSSAVNGRAASRAA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=407, Percent_Identity=46.1916461916462, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI4557737, Length=400, Percent_Identity=45, Blast_Score=301, Evalue=6e-82,
Organism=Escherichia coli, GI1789311, Length=409, Percent_Identity=48.6552567237164, Blast_Score=356, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17538498, Length=402, Percent_Identity=45.5223880597015, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17538494, Length=402, Percent_Identity=44.7761194029851, Blast_Score=294, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI25145633, Length=404, Percent_Identity=44.3069306930693, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17551082, Length=406, Percent_Identity=43.3497536945813, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI32565911, Length=320, Percent_Identity=47.8125, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI25145635, Length=319, Percent_Identity=46.7084639498433, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI32565905, Length=330, Percent_Identity=46.3636363636364, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI32565903, Length=304, Percent_Identity=48.3552631578947, Blast_Score=238, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6320710, Length=403, Percent_Identity=44.9131513647643, Blast_Score=306, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6323209, Length=411, Percent_Identity=44.7688564476886, Blast_Score=306, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24580527, Length=412, Percent_Identity=45.3883495145631, Blast_Score=295, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24580525, Length=412, Percent_Identity=45.3883495145631, Blast_Score=295, Evalue=3e-80,
Organism=Drosophila melanogaster, GI45552159, Length=412, Percent_Identity=45.3883495145631, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24580529, Length=412, Percent_Identity=45.3883495145631, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24580537, Length=412, Percent_Identity=45.3883495145631, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24580533, Length=412, Percent_Identity=45.3883495145631, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24580535, Length=412, Percent_Identity=45.3883495145631, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24580531, Length=412, Percent_Identity=45.3883495145631, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24580541, Length=397, Percent_Identity=44.3324937027708, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24580539, Length=142, Percent_Identity=37.3239436619718, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 46046; Mature: 46046

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRANYLFTSESVAEGHPDKVCDRISDEIVDLVYREAAKTGVDPWTVRIACETLATTNRVV
CCCCEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHCCCCEEE
IAGEVRLPPSLLKKDKNGNDVINPSKFKSAARKAIRDIGYEQDGFHWKTAKIDVLLHFQS
EEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECH
AHIAQGVDNASDKQGEEGAGDQGIMFGYACRETAELMPAPIYYSHRILNLLAAARKKGEG
HHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
EVAKLGPDAKSQVTVRYIDGKPAEVTSIVLSTQHLDDSWDSAKVRSVVEPYIHEALGDLK
CEEECCCCCCCEEEEEEECCCCCHHHEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCE
IADDCTWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAA
EECCEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
YAARYLAKNVVAAGLAERCTIQLSYAIGVAQPLSIYVDLHGTGKVSEDQVENAIRKTMDL
HHHHHHHHHHHHHCCHHHEEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCC
SPTGIRRHLDLNRPIYAKTSAYGHFGRKAGRDGSFSWEKLDLVKPLKEAIESDSSAVNGR
CCCHHHHHCCCCCCEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
AASRAA
CCCCCC
>Mature Secondary Structure
MRANYLFTSESVAEGHPDKVCDRISDEIVDLVYREAAKTGVDPWTVRIACETLATTNRVV
CCCCEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHCCCCEEE
IAGEVRLPPSLLKKDKNGNDVINPSKFKSAARKAIRDIGYEQDGFHWKTAKIDVLLHFQS
EEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECH
AHIAQGVDNASDKQGEEGAGDQGIMFGYACRETAELMPAPIYYSHRILNLLAAARKKGEG
HHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
EVAKLGPDAKSQVTVRYIDGKPAEVTSIVLSTQHLDDSWDSAKVRSVVEPYIHEALGDLK
CEEECCCCCCCEEEEEEECCCCCHHHEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCE
IADDCTWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAA
EECCEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
YAARYLAKNVVAAGLAERCTIQLSYAIGVAQPLSIYVDLHGTGKVSEDQVENAIRKTMDL
HHHHHHHHHHHHHCCHHHEEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCC
SPTGIRRHLDLNRPIYAKTSAYGHFGRKAGRDGSFSWEKLDLVKPLKEAIESDSSAVNGR
CCCHHHHHCCCCCCEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
AASRAA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA