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The map label for this gene is mtlF

Identifier: 150394668

GI number: 150394668

Start: 2315896

End: 2316330

Strand: Direct

Name: mtlF

Synonym: SaurJH1_2228

Alternate gene names: 150394668

Gene position: 2315896-2316330 (Clockwise)

Preceding gene: 150394667

Following gene: 150394669

Centisome position: 79.68

GC content: 35.63

Gene sequence:

>435_bases
ATGAGCGAATTATTTAGTAATGACAATATCTTTTTAAATGTAAATGTTAACAGCCAAAATGAAGCAATTGAAAAAGCAGG
TAAAGCCTTAGTTGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTTGTATCAACAT
TTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTA
CAAATCCCTGAAGGCGTTGACTGGGATGGCGAAGTAGTTAAAGTTGTAGTGGGAATCGCTGGTAAAGATGGCGAACATTT
AGACTTGTTATCTAAAATTGCAATTACATTTAGCGAAGAAGAAAATGTGGATCGTATCGTTCAAGCAAAATCTGCAGAAG
AAATTAAACAAGTATTCGAGGAGGCAGATGCATAA

Upstream 100 bases:

>100_bases
CTATTGATACTTTTATGGAAAATCCACAGGAACTTGAAACATTATTAAGAAACAAATTTTTAGAACGAATTAAAAAACAA
TTTATTTAGGAGTGTACAAA

Downstream 100 bases:

>100_bases
TGAAAGCAGTTCACTTTGGTGCTGGTAACATAGGTCGTGGTTTCATTGGTTATATTCTTGCAGACAACAATGTTAAAGTA
ACATTTGCAGACGTCAATGA

Product: phosphoenolpyruvate-dependent sugar phosphotransferase system EIIA 2

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAMKDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLL
QIPEGVDWDGEVVKVVVGIAGKDGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA

Sequences:

>Translated_144_residues
MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAMKDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLL
QIPEGVDWDGEVVKVVVGIAGKDGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA
>Mature_143_residues
SELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAMKDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQ
IPEGVDWDGEVVKVVVGIAGKDGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain

Homologues:

Organism=Escherichia coli, GI1788494, Length=122, Percent_Identity=40.9836065573771, Blast_Score=105, Evalue=9e-25,
Organism=Escherichia coli, GI1790027, Length=135, Percent_Identity=35.5555555555556, Blast_Score=102, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTMA_STAAC (Q5HE46)

Other databases:

- EMBL:   Y09927
- EMBL:   CP000046
- RefSeq:   YP_186961.1
- ProteinModelPortal:   Q5HE46
- SMR:   Q5HE46
- STRING:   Q5HE46
- EnsemblBacteria:   EBSTAT00000008461
- GeneID:   3237440
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL2148
- TIGR:   SACOL2148
- eggNOG:   COG4668
- GeneTree:   EBGT00050000023803
- HOGENOM:   HBG697201
- OMA:   GDEHLEI
- ProtClustDB:   CLSK885798
- BioCyc:   SAUR93062:SACOL2148-MONOMER
- GO:   GO:0005737
- InterPro:   IPR016152
- InterPro:   IPR002178
- Gene3D:   G3DSA:3.40.930.10

Pfam domain/function: PF00359 PTS_EIIA_2; SSF55804 PTrfase/Anion_transptr

EC number: 2.7.1.69

Molecular weight: Translated: 15543; Mature: 15411

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: PS51094 PTS_EIIA_TYPE_2; PS00372 PTS_EIIA_TYPE_2_HIS

Important sites: ACT_SITE 63-63

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAMKDREQVVSTFMGNGLA
CCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
IPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGKDGEHLDLLSKIAITFSEE
CCCCCCHHHHHHHHCCCEEEECCCCCCCCCCEEEHEEECCCCCCCHHHHHHHHHHHCCCC
ENVDRIVQAKSAEEIKQVFEEADA
CCHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAMKDREQVVSTFMGNGLA
CCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
IPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGKDGEHLDLLSKIAITFSEE
CCCCCCHHHHHHHHCCCEEEECCCCCCCCCCEEEHEEECCCCCCCHHHHHHHHHHHCCCC
ENVDRIVQAKSAEEIKQVFEEADA
CCHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA