Click here to switch to the map view.

The map label for this gene is mbhs

Identifier: 15004844

GI number: 15004844

Start: 151995

End: 152870

Strand: Direct

Name: mbhs

Synonym: CA_P0141

Alternate gene names: 15004844

Gene position: 151995-152870 (Clockwise)

Preceding gene: 15004843

Following gene: 15004845

Centisome position: 79.16

GC content: 36.3

Gene sequence:

>876_bases
ATGAAAAATGAAAATCATTGTCCTATGACCGAAGAAAAAAAAGCCACTTCAAAAACCCTTGTAAATGAAGCAATAACACT
AATCAAAAATAGAAATGCAAAAAAGCTAAATGCCATATGGCTTGAGGTTACAGGGTGCTCTGGAAATATTATTTCCTTTT
TAAATAGTGAAAATCCAGATTTAAATTATGTATTAAATCAGCTTATAAACCTAACTTATAATAACACCTTAATGACTGCT
GAAGGAGAAGCTGCTTTTGAGACTTTTCTAAATACCTTAAACACAGAATTTATACTTTTAGTAGATGGAGCTGTTTCTGC
CAAAGAAAATGGCCAATATAATATTGTTGCCCACTACAAAGACAAGCGTATTACAGCACTAGAAGCAGTAAAAATGGCAG
GAGAAAAAGCAAAATACGTACTAGCTGTAGGCACCTGTGCTTCTTCCGGTGGTATTTCCGCTGCAAAACCTAATCCTTCT
TTAAGCAAAAGCATATCGGAGGTCATAAATCACGAAGTTATAAATCTTCCAGGATGTCCTTGTCATCCCGATTGGGTAGT
AGGAACACTGGCTCATCTAGTAGCATTTGGAAAGCCTGAACTTGATGCCTACAAACGCCCCATTTTATTTTATGGATCAA
CTATTCATGACAATTGTACAAGACGAGGATATTTTGACCAAAGAATATTTGCAAAAAAGTTTGGTGAAAAGGGCTGCATG
TTTAAATTGGGCTGCCGTGGTCCTGTAACGAAAACAGATTGTCCAAGAAGAAAATGGAATGGCTATGTTAACTGGCCAGT
TGAAGACAATACAACCTGTATAGGCTGTGCCAATTTCAATTTTCCAGATGGCATGGAACCATTTATAAGATACTAA

Upstream 100 bases:

>100_bases
ATTTTCAAATAAAACATAGTCCCTTTTCTACCTTCTTTCTTCCGTATATTTTCCTTAACTAAAGAATAGATTTAAATATA
TCTATTTTGTGAGGCTATAT

Downstream 100 bases:

>100_bases
TTTTTTAGGAGGCTCTCTTGAAAAAAACTATTATAATCGATCCTATAACAAGAATTAGCGGTTTTCTTGAAACCAAAGTA
GAAGTTACTGAAAGCGTAAT

Product: periplasmic hydrogenase small subunit, dehydrogenase

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKNENHCPMTEEKKATSKTLVNEAITLIKNRNAKKLNAIWLEVTGCSGNIISFLNSENPDLNYVLNQLINLTYNNTLMTA
EGEAAFETFLNTLNTEFILLVDGAVSAKENGQYNIVAHYKDKRITALEAVKMAGEKAKYVLAVGTCASSGGISAAKPNPS
LSKSISEVINHEVINLPGCPCHPDWVVGTLAHLVAFGKPELDAYKRPILFYGSTIHDNCTRRGYFDQRIFAKKFGEKGCM
FKLGCRGPVTKTDCPRRKWNGYVNWPVEDNTTCIGCANFNFPDGMEPFIRY

Sequences:

>Translated_291_residues
MKNENHCPMTEEKKATSKTLVNEAITLIKNRNAKKLNAIWLEVTGCSGNIISFLNSENPDLNYVLNQLINLTYNNTLMTA
EGEAAFETFLNTLNTEFILLVDGAVSAKENGQYNIVAHYKDKRITALEAVKMAGEKAKYVLAVGTCASSGGISAAKPNPS
LSKSISEVINHEVINLPGCPCHPDWVVGTLAHLVAFGKPELDAYKRPILFYGSTIHDNCTRRGYFDQRIFAKKFGEKGCM
FKLGCRGPVTKTDCPRRKWNGYVNWPVEDNTTCIGCANFNFPDGMEPFIRY
>Mature_291_residues
MKNENHCPMTEEKKATSKTLVNEAITLIKNRNAKKLNAIWLEVTGCSGNIISFLNSENPDLNYVLNQLINLTYNNTLMTA
EGEAAFETFLNTLNTEFILLVDGAVSAKENGQYNIVAHYKDKRITALEAVKMAGEKAKYVLAVGTCASSGGISAAKPNPS
LSKSISEVINHEVINLPGCPCHPDWVVGTLAHLVAFGKPELDAYKRPILFYGSTIHDNCTRRGYFDQRIFAKKFGEKGCM
FKLGCRGPVTKTDCPRRKWNGYVNWPVEDNTTCIGCANFNFPDGMEPFIRY

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=262, Percent_Identity=34.3511450381679, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1789371, Length=256, Percent_Identity=34.765625, Blast_Score=145, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 32303; Mature: 32303

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNENHCPMTEEKKATSKTLVNEAITLIKNRNAKKLNAIWLEVTGCSGNIISFLNSENPD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHCCCCCCCC
LNYVLNQLINLTYNNTLMTAEGEAAFETFLNTLNTEFILLVDGAVSAKENGQYNIVAHYK
HHHHHHHHHHCEECCEEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEEC
DKRITALEAVKMAGEKAKYVLAVGTCASSGGISAAKPNPSLSKSISEVINHEVINLPGCP
CCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCC
CHPDWVVGTLAHLVAFGKPELDAYKRPILFYGSTIHDNCTRRGYFDQRIFAKKFGEKGCM
CCCHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCE
FKLGCRGPVTKTDCPRRKWNGYVNWPVEDNTTCIGCANFNFPDGMEPFIRY
EEECCCCCCCCCCCCHHHCCCEEECCCCCCCEEEEEECCCCCCCCHHHHCC
>Mature Secondary Structure
MKNENHCPMTEEKKATSKTLVNEAITLIKNRNAKKLNAIWLEVTGCSGNIISFLNSENPD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHCCCCCCCC
LNYVLNQLINLTYNNTLMTAEGEAAFETFLNTLNTEFILLVDGAVSAKENGQYNIVAHYK
HHHHHHHHHHCEECCEEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEEC
DKRITALEAVKMAGEKAKYVLAVGTCASSGGISAAKPNPSLSKSISEVINHEVINLPGCP
CCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCC
CHPDWVVGTLAHLVAFGKPELDAYKRPILFYGSTIHDNCTRRGYFDQRIFAKKFGEKGCM
CCCHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCE
FKLGCRGPVTKTDCPRRKWNGYVNWPVEDNTTCIGCANFNFPDGMEPFIRY
EEECCCCCCCCCCCCHHHCCCEEECCCCCCCEEEEEECCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]