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The map label for this gene is mbhl

Identifier: 15004845

GI number: 15004845

Start: 152888

End: 154303

Strand: Direct

Name: mbhl

Synonym: CA_P0142

Alternate gene names: 15004845

Gene position: 152888-154303 (Clockwise)

Preceding gene: 15004844

Following gene: 15004846

Centisome position: 79.63

GC content: 35.03

Gene sequence:

>1416_bases
TTGAAAAAAACTATTATAATCGATCCTATAACAAGAATTAGCGGTTTTCTTGAAACCAAAGTAGAAGTTACTGAAAGCGT
AATTACAAATGCTGAAACCAGTGGTCTTCTATTTAGAGGCTTTGAAAAGATGCTTAAAACTCGTCAACCACTTGATGCTA
TTTATTTTACTGAAAGAATTTGCGGAATATGTTCTACTGCCCATGCTATGGCATCAACTTTAGCACTAGAGGATGCCTTA
AAGGTTTCGGTCAGCATAAATGACTTATACGTACGTGATTTAATTCACAGCTTTGAATTTATGCAAAATCACATAAGACA
GTTTTATAATCTAACAGTTCCAAGCTATGTAAGAATGTCAAAGCTAGATCCTTTATATTCAAACCAATATAGTGATTATA
GGCTTCCTAATTATGAAAACGAAATTATTTCTAAGCACAGTTTAGAAAGTATTAATTACAGCCGTCTTGCCCATGAAGGT
CTAGCTCTTTTCGGAGGAAAAGCTCCTCACTCCCATGGTATTTTTGTTGGGGGAGTTACAACTAGCATAGATTCCTACAA
ACTAGTAAAAGCAAAATCCATAATCTCAAGCCTAAAAGTATTTATAAATAACATTATGCTTGAGGATATGAATACCATAG
CAAAATACTACTCAGACTACTTTAACATGGGCGAAACCTACAATAACTTCATGAGCTATGGACTTTTTGATCACTATAAA
GATCCTGAAATAAGCTATGTATCTCCAAGTGTAATGATTAATTATAAAAAGCAGAGCTTTGACAAAAACAAAATCACAGA
AAACATACTTCACAGCTGGTATGTTAGCGATACGGAAAATGAAAAACCTTCAAATGAAAGCGCCGACAATATTGATTTAA
ATAAAAAACAAGGTTACAGCTTTATAAAATCTCCCAATTACGACGGTTATCCCATGGAGGTTGGACCACTAGCTAGAATG
ATACTTTCTGGTGATTACAAAGGCAAAAGCTCCTGCATGGATAGAAATGTCGCTCGAGTTTTAGAAGCAAAAAAAATAAT
CTCTATTATGGAAAAGCTTGCAGATAAAATTGAGCTTCTACCAAACTCCCAAAAGACATACGATATACCAATAAACGCTT
TTGGTGCTGGTCTAACTGATACTACACGGGGAGCTTTAGGTCATTGGATTAAGATAGAAAACAAGCTTATAAAGTACTAT
AACATAATAACTCCAACAGTATGGAATATGAGTCCCAAGAATGAAAAAGGGGCTTTTGGGGTTGGTGAAAAATCCTTGGT
TGGAACCAAGCTAAAAAATGTTCAAGAACCAGTAGAAGTTGGGAGAATTATGCGCTCCTTTGATCCCTGCGTTTCCTGTG
CTACTCATTTAGTAAGCGATAAATACAGTCCTATAGACATCAAGGTAATAGTATGA

Upstream 100 bases:

>100_bases
GGCCAGTTGAAGACAATACAACCTGTATAGGCTGTGCCAATTTCAATTTTCCAGATGGCATGGAACCATTTATAAGATAC
TAATTTTTTAGGAGGCTCTC

Downstream 100 bases:

>100_bases
TAAAGGTTATTGCCCTTGGAAACACACTAATGGAGGATGATGGGATTGGAATTGTGGTTGTAAACAAACTTAAGGAAACG
CTTGAGAAAAACAATATTGA

Product: periplasmic hydrogenase large subunit, dehydrogenase

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MKKTIIIDPITRISGFLETKVEVTESVITNAETSGLLFRGFEKMLKTRQPLDAIYFTERICGICSTAHAMASTLALEDAL
KVSVSINDLYVRDLIHSFEFMQNHIRQFYNLTVPSYVRMSKLDPLYSNQYSDYRLPNYENEIISKHSLESINYSRLAHEG
LALFGGKAPHSHGIFVGGVTTSIDSYKLVKAKSIISSLKVFINNIMLEDMNTIAKYYSDYFNMGETYNNFMSYGLFDHYK
DPEISYVSPSVMINYKKQSFDKNKITENILHSWYVSDTENEKPSNESADNIDLNKKQGYSFIKSPNYDGYPMEVGPLARM
ILSGDYKGKSSCMDRNVARVLEAKKIISIMEKLADKIELLPNSQKTYDIPINAFGAGLTDTTRGALGHWIKIENKLIKYY
NIITPTVWNMSPKNEKGAFGVGEKSLVGTKLKNVQEPVEVGRIMRSFDPCVSCATHLVSDKYSPIDIKVIV

Sequences:

>Translated_471_residues
MKKTIIIDPITRISGFLETKVEVTESVITNAETSGLLFRGFEKMLKTRQPLDAIYFTERICGICSTAHAMASTLALEDAL
KVSVSINDLYVRDLIHSFEFMQNHIRQFYNLTVPSYVRMSKLDPLYSNQYSDYRLPNYENEIISKHSLESINYSRLAHEG
LALFGGKAPHSHGIFVGGVTTSIDSYKLVKAKSIISSLKVFINNIMLEDMNTIAKYYSDYFNMGETYNNFMSYGLFDHYK
DPEISYVSPSVMINYKKQSFDKNKITENILHSWYVSDTENEKPSNESADNIDLNKKQGYSFIKSPNYDGYPMEVGPLARM
ILSGDYKGKSSCMDRNVARVLEAKKIISIMEKLADKIELLPNSQKTYDIPINAFGAGLTDTTRGALGHWIKIENKLIKYY
NIITPTVWNMSPKNEKGAFGVGEKSLVGTKLKNVQEPVEVGRIMRSFDPCVSCATHLVSDKYSPIDIKVIV
>Mature_471_residues
MKKTIIIDPITRISGFLETKVEVTESVITNAETSGLLFRGFEKMLKTRQPLDAIYFTERICGICSTAHAMASTLALEDAL
KVSVSINDLYVRDLIHSFEFMQNHIRQFYNLTVPSYVRMSKLDPLYSNQYSDYRLPNYENEIISKHSLESINYSRLAHEG
LALFGGKAPHSHGIFVGGVTTSIDSYKLVKAKSIISSLKVFINNIMLEDMNTIAKYYSDYFNMGETYNNFMSYGLFDHYK
DPEISYVSPSVMINYKKQSFDKNKITENILHSWYVSDTENEKPSNESADNIDLNKKQGYSFIKSPNYDGYPMEVGPLARM
ILSGDYKGKSSCMDRNVARVLEAKKIISIMEKLADKIELLPNSQKTYDIPINAFGAGLTDTTRGALGHWIKIENKLIKYY
NIITPTVWNMSPKNEKGAFGVGEKSLVGTKLKNVQEPVEVGRIMRSFDPCVSCATHLVSDKYSPIDIKVIV

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=573, Percent_Identity=29.1448516579407, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1789368, Length=570, Percent_Identity=27.8947368421053, Blast_Score=226, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 53333; Mature: 53333

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTIIIDPITRISGFLETKVEVTESVITNAETSGLLFRGFEKMLKTRQPLDAIYFTERI
CCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
CGICSTAHAMASTLALEDALKVSVSINDLYVRDLIHSFEFMQNHIRQFYNLTVPSYVRMS
HHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KLDPLYSNQYSDYRLPNYENEIISKHSLESINYSRLAHEGLALFGGKAPHSHGIFVGGVT
HCCCHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEECCCC
TSIDSYKLVKAKSIISSLKVFINNIMLEDMNTIAKYYSDYFNMGETYNNFMSYGLFDHYK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHCCC
DPEISYVSPSVMINYKKQSFDKNKITENILHSWYVSDTENEKPSNESADNIDLNKKQGYS
CCCCEEECCCEEEEEHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCH
FIKSPNYDGYPMEVGPLARMILSGDYKGKSSCMDRNVARVLEAKKIISIMEKLADKIELL
HHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PNSQKTYDIPINAFGAGLTDTTRGALGHWIKIENKLIKYYNIITPTVWNMSPKNEKGAFG
CCCCCEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCC
VGEKSLVGTKLKNVQEPVEVGRIMRSFDPCVSCATHLVSDKYSPIDIKVIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MKKTIIIDPITRISGFLETKVEVTESVITNAETSGLLFRGFEKMLKTRQPLDAIYFTERI
CCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
CGICSTAHAMASTLALEDALKVSVSINDLYVRDLIHSFEFMQNHIRQFYNLTVPSYVRMS
HHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KLDPLYSNQYSDYRLPNYENEIISKHSLESINYSRLAHEGLALFGGKAPHSHGIFVGGVT
HCCCHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEECCCC
TSIDSYKLVKAKSIISSLKVFINNIMLEDMNTIAKYYSDYFNMGETYNNFMSYGLFDHYK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHCCC
DPEISYVSPSVMINYKKQSFDKNKITENILHSWYVSDTENEKPSNESADNIDLNKKQGYS
CCCCEEECCCEEEEEHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCH
FIKSPNYDGYPMEVGPLARMILSGDYKGKSSCMDRNVARVLEAKKIISIMEKLADKIELL
HHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PNSQKTYDIPINAFGAGLTDTTRGALGHWIKIENKLIKYYNIITPTVWNMSPKNEKGAFG
CCCCCEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCC
VGEKSLVGTKLKNVQEPVEVGRIMRSFDPCVSCATHLVSDKYSPIDIKVIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]