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The map label for this gene is ispG

Identifier: 150009758

GI number: 150009758

Start: 3837214

End: 3839049

Strand: Direct

Name: ispG

Synonym: BDI_3173

Alternate gene names: 150009758

Gene position: 3837214-3839049 (Clockwise)

Preceding gene: 150009757

Following gene: 150009759

Centisome position: 79.75

GC content: 50.0

Gene sequence:

>1836_bases
ATGGCTTACTTTAATTACAACCGCCGTAAATCATCCGTTGCCCAAATCGGTGACACACCCATGGGAGGTGAGAATCCTAT
CCGTATTCAATCCATGGCTAATGTATCAACGATGGATACGGAGGCGGCGGTAGCTCAAGCGATCCGTATGATCGAGGCGG
GAGCTGAGTATGTGCGTTTTACGGCACAGGGGGAACGGGAAGCCCGAAACTTGGGCGAGATTCGTAAACAATTGAATGAG
CAAGGGTATACGACTCCTTTGGTCGCTGATATCCATTTTAATCCTAGAGCGGCTGATGCCGCCGCCGGTGAGGTGGAAAA
GGTACGTATCAATCCGGGAAACTATGTAGATAAGGTAAAGACATTCAGCCATCTTGAATATACGGATGAGGAATATGCCG
CCGAGATCGAGAAGATCCGCGAGCGGTTCGTCCCTTTCTTGAATATTTGTAAGGCACATGGCACCGCTATTCGTATCGGT
GTGAATCATGGCTCGCTCTCCGATCGTATCATGAGCCGTTATGGTGATACGCCCGAGGGGATGGTAGCGAGTTGTATGGA
GTTTCTTCGCATTTGCCGGGAGGAGAATTTCCCGGATGTGGTTATTTCGATTAAGGCTTCCAATACCGTTGTCATGGTAA
GGACCGTGCGGTTGTTGGTTCGTACGATGGAGGCCGAGAATATGCATTACCCCTTGCACCTTGGGGTAACGGAGGCGGGT
GACGGTGAGGACGGGCGTATCAAGAGCGCTGTTGGAATCGGTACGCTTCTATGTGACGGTATCGGTGATACGATCCGGGT
TTCCTTGAGTGAGGACCCCGAGGCGGAAATGCCGGTAGCGCGTAAATTGGTGGATTATATTAGGGAACGGGAAAATCATC
GTCCTATCGAGGCTTCGATGGCTCCCGGTTTCGATACGGTGGCGACAAGCCGTCGTATAAGCCGTGTGGTGGAAGGTATC
GGAGGTACTTTCTCGCCAGTCGTGATCTCCGATCGTAGTAGCGGGGATTTTGAGTTTGATTATTTGTCCTTGCCGGACTA
TATATATATAGGAAAGGAAGATCCGGATAATTTGCCGGATAATTTCCGTTTATTGGTAGACGCTCATTTTTGGAAAGAGC
GTCCGAATGCTTTTCCTTGCTTCATCGCCTCGGAGGCGGAGGAGTTAAAGGATTACGATTGTCCGCTTAAGTTTATCCGT
CTGACGTATATGGATCTTACGGATCGTATGTTGGAGATCTTGAAAGCGGATAAGACCGTGGTGGTCTTATTAAGTACACA
TCATCGGAATGGGGTTGGCTCGCAACGTGCGGCTATGCATAAATTGCTGATGGCGGGTTGTGACGTGCCGGTTGTCTTGC
ATCGTGATTTCCGGGAGACGGATGTGGAGTTGTTGCAACTGAAATCCGCCGCCGATTTCGGAACGCTTTTGCTGGATGGT
TTCGGGGATGGCTTGATGCTGCACAATGAGGGATGCGAGGCGGTTGTCTCGGATCGCTGTATGTTCGGGATCTTGCAGGC
TACAAGAACCCGTATCAGTAAGACGGAATATATCTCTTGTCCGAGTTGCGGACGTACCTTGTACGACCTGCAAACAACGA
TCGCCCGTATCAAGGAGGCTACTTCCCATTTAAAAGGCTTGAAGATCGGTATCATGGGCTGTATCGTGAATGGTCCCGGC
GAGATGGCCGATGCCGACTATGGTTATGTGGGTGCCGGACGTGGACAAATCAGCCTGTATAAAGGTAAGGAATGTGTCCT
CAAGAATATCCCCGAGGAAGACGCTGTAGAACGTTTAGTTCAGTTAATAAAAGAGAACGGGGATTGGGTTAATTGA

Upstream 100 bases:

>100_bases
GTTGAAAGTTGAGAATTGAAAATTAAGGAGAAAGTCTCTCTTTAGGTTTTCAGTTCTCAACACTCATTCTCAATTTTCAA
TTCTCAATTTTCAATGAACA

Downstream 100 bases:

>100_bases
TTGACTATTGACAATTTGTTTATCTTTGCAAGTATGAAAGTAAGAATTATCAATAAATCGCATCACCCGCTGCCGGGTTA
TGCGACTCCTTTATCTGCCG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MAYFNYNRRKSSVAQIGDTPMGGENPIRIQSMANVSTMDTEAAVAQAIRMIEAGAEYVRFTAQGEREARNLGEIRKQLNE
QGYTTPLVADIHFNPRAADAAAGEVEKVRINPGNYVDKVKTFSHLEYTDEEYAAEIEKIRERFVPFLNICKAHGTAIRIG
VNHGSLSDRIMSRYGDTPEGMVASCMEFLRICREENFPDVVISIKASNTVVMVRTVRLLVRTMEAENMHYPLHLGVTEAG
DGEDGRIKSAVGIGTLLCDGIGDTIRVSLSEDPEAEMPVARKLVDYIRERENHRPIEASMAPGFDTVATSRRISRVVEGI
GGTFSPVVISDRSSGDFEFDYLSLPDYIYIGKEDPDNLPDNFRLLVDAHFWKERPNAFPCFIASEAEELKDYDCPLKFIR
LTYMDLTDRMLEILKADKTVVVLLSTHHRNGVGSQRAAMHKLLMAGCDVPVVLHRDFRETDVELLQLKSAADFGTLLLDG
FGDGLMLHNEGCEAVVSDRCMFGILQATRTRISKTEYISCPSCGRTLYDLQTTIARIKEATSHLKGLKIGIMGCIVNGPG
EMADADYGYVGAGRGQISLYKGKECVLKNIPEEDAVERLVQLIKENGDWVN

Sequences:

>Translated_611_residues
MAYFNYNRRKSSVAQIGDTPMGGENPIRIQSMANVSTMDTEAAVAQAIRMIEAGAEYVRFTAQGEREARNLGEIRKQLNE
QGYTTPLVADIHFNPRAADAAAGEVEKVRINPGNYVDKVKTFSHLEYTDEEYAAEIEKIRERFVPFLNICKAHGTAIRIG
VNHGSLSDRIMSRYGDTPEGMVASCMEFLRICREENFPDVVISIKASNTVVMVRTVRLLVRTMEAENMHYPLHLGVTEAG
DGEDGRIKSAVGIGTLLCDGIGDTIRVSLSEDPEAEMPVARKLVDYIRERENHRPIEASMAPGFDTVATSRRISRVVEGI
GGTFSPVVISDRSSGDFEFDYLSLPDYIYIGKEDPDNLPDNFRLLVDAHFWKERPNAFPCFIASEAEELKDYDCPLKFIR
LTYMDLTDRMLEILKADKTVVVLLSTHHRNGVGSQRAAMHKLLMAGCDVPVVLHRDFRETDVELLQLKSAADFGTLLLDG
FGDGLMLHNEGCEAVVSDRCMFGILQATRTRISKTEYISCPSCGRTLYDLQTTIARIKEATSHLKGLKIGIMGCIVNGPG
EMADADYGYVGAGRGQISLYKGKECVLKNIPEEDAVERLVQLIKENGDWVN
>Mature_610_residues
AYFNYNRRKSSVAQIGDTPMGGENPIRIQSMANVSTMDTEAAVAQAIRMIEAGAEYVRFTAQGEREARNLGEIRKQLNEQ
GYTTPLVADIHFNPRAADAAAGEVEKVRINPGNYVDKVKTFSHLEYTDEEYAAEIEKIRERFVPFLNICKAHGTAIRIGV
NHGSLSDRIMSRYGDTPEGMVASCMEFLRICREENFPDVVISIKASNTVVMVRTVRLLVRTMEAENMHYPLHLGVTEAGD
GEDGRIKSAVGIGTLLCDGIGDTIRVSLSEDPEAEMPVARKLVDYIRERENHRPIEASMAPGFDTVATSRRISRVVEGIG
GTFSPVVISDRSSGDFEFDYLSLPDYIYIGKEDPDNLPDNFRLLVDAHFWKERPNAFPCFIASEAEELKDYDCPLKFIRL
TYMDLTDRMLEILKADKTVVVLLSTHHRNGVGSQRAAMHKLLMAGCDVPVVLHRDFRETDVELLQLKSAADFGTLLLDGF
GDGLMLHNEGCEAVVSDRCMFGILQATRTRISKTEYISCPSCGRTLYDLQTTIARIKEATSHLKGLKIGIMGCIVNGPGE
MADADYGYVGAGRGQISLYKGKECVLKNIPEEDAVERLVQLIKENGDWVN

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=321, Percent_Identity=35.5140186915888, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_PARD8 (A6LGR5)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001304501.1
- STRING:   A6LGR5
- GeneID:   5308322
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_3173
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   SMRIGTN
- ProtClustDB:   PRK02048
- BioCyc:   PDIS435591:BDI_3173-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR011005
- InterPro:   IPR017178
- InterPro:   IPR004588
- PIRSF:   PIRSF037336
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 67948; Mature: 67817

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYFNYNRRKSSVAQIGDTPMGGENPIRIQSMANVSTMDTEAAVAQAIRMIEAGAEYVRF
CCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHEEE
TAQGEREARNLGEIRKQLNEQGYTTPLVADIHFNPRAADAAAGEVEKVRINPGNYVDKVK
ECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHH
TFSHLEYTDEEYAAEIEKIRERFVPFLNICKAHGTAIRIGVNHGSLSDRIMSRYGDTPEG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCH
MVASCMEFLRICREENFPDVVISIKASNTVVMVRTVRLLVRTMEAENMHYPLHLGVTEAG
HHHHHHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCEEEEECCCCC
DGEDGRIKSAVGIGTLLCDGIGDTIRVSLSEDPEAEMPVARKLVDYIRERENHRPIEASM
CCCCCCEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
APGFDTVATSRRISRVVEGIGGTFSPVVISDRSSGDFEFDYLSLPDYIYIGKEDPDNLPD
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEECCCCEEEECCCCCCCCCC
NFRLLVDAHFWKERPNAFPCFIASEAEELKDYDCPLKFIRLTYMDLTDRMLEILKADKTV
CEEEEEEEHHHHCCCCCCEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
VVLLSTHHRNGVGSQRAAMHKLLMAGCDVPVVLHRDFRETDVELLQLKSAADFGTLLLDG
EEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCCCHHEEEC
FGDGLMLHNEGCEAVVSDRCMFGILQATRTRISKTEYISCPSCGRTLYDLQTTIARIKEA
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHH
TSHLKGLKIGIMGCIVNGPGEMADADYGYVGAGRGQISLYKGKECVLKNIPEEDAVERLV
HHHHCCCEEEEEEEEECCCCCCCCCCCCEEECCCCEEEEECCCHHHHHCCCCHHHHHHHH
QLIKENGDWVN
HHHHCCCCCCC
>Mature Secondary Structure 
AYFNYNRRKSSVAQIGDTPMGGENPIRIQSMANVSTMDTEAAVAQAIRMIEAGAEYVRF
CCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHEEE
TAQGEREARNLGEIRKQLNEQGYTTPLVADIHFNPRAADAAAGEVEKVRINPGNYVDKVK
ECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHH
TFSHLEYTDEEYAAEIEKIRERFVPFLNICKAHGTAIRIGVNHGSLSDRIMSRYGDTPEG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCH
MVASCMEFLRICREENFPDVVISIKASNTVVMVRTVRLLVRTMEAENMHYPLHLGVTEAG
HHHHHHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCEEEEECCCCC
DGEDGRIKSAVGIGTLLCDGIGDTIRVSLSEDPEAEMPVARKLVDYIRERENHRPIEASM
CCCCCCEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
APGFDTVATSRRISRVVEGIGGTFSPVVISDRSSGDFEFDYLSLPDYIYIGKEDPDNLPD
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEECCCCEEEECCCCCCCCCC
NFRLLVDAHFWKERPNAFPCFIASEAEELKDYDCPLKFIRLTYMDLTDRMLEILKADKTV
CEEEEEEEHHHHCCCCCCEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
VVLLSTHHRNGVGSQRAAMHKLLMAGCDVPVVLHRDFRETDVELLQLKSAADFGTLLLDG
EEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCCCHHEEEC
FGDGLMLHNEGCEAVVSDRCMFGILQATRTRISKTEYISCPSCGRTLYDLQTTIARIKEA
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHH
TSHLKGLKIGIMGCIVNGPGEMADADYGYVGAGRGQISLYKGKECVLKNIPEEDAVERLV
HHHHCCCEEEEEEEEECCCCCCCCCCCCEEECCCCEEEEECCCHHHHHCCCCHHHHHHHH
QLIKENGDWVN
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA