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The map label for this gene is ppdK [H]

Identifier: 150005862

GI number: 150005862

Start: 4198050

End: 4200770

Strand: Direct

Name: ppdK [H]

Synonym: BVU_3358

Alternate gene names: 150005862

Gene position: 4198050-4200770 (Clockwise)

Preceding gene: 150005859

Following gene: 150005863

Centisome position: 81.31

GC content: 47.08

Gene sequence:

>2721_bases
ATGGATAAAAAAAGAGTTTACACCTTTGGTAATGGACAAGCTGAAGGAAAAGCAGACATGAGAAATCTGCTTGGTGGAAA
AGGCGCTAACCTGGCTGAAATGAATCTAATCGGTGTTCCCGTACCTCCGGGATTTACAATCACCACAGAAGTTTGTAGCG
AATATTATGAGTTAGGTAAAGATAAAGTAGTAGAACTGCTGAAAGCGGATGTTGAGAAAGCTATCGCCAACATCGAAAAT
CTGATGAATTCCAAGTTTGGAGATGTAGAGAACCCGTTACTGGTTTCTGTACGTTCGGGTGCCCGCGCTTCTATGCCGGG
TATGATGGATACCATCCTTAATTTAGGTTTAAATGACGAAGTGGTGGAAGGCTTGAGCCGCAAGACCGGACGCCCCCGTT
TCGCATGGGATTCTTACCGTCGTTTCGTACAAATGTATGGTGACGTGGTATTGGGTATGAAACCGGTGAACAAGGAAGAC
ATCGATCCTTTCGAGGAAATTATCGAAAAAGTAAAGGAAGAAAAAGGTGTGAAGCTCGACAACGAATTGGAAGTGGAAGA
CCTTAAAGAACTGGTTAAACGCTTTAAAGTTGCTGTAAAAGAACAGACCGGACAAGATTTTCCGACCTGTGCATACGAAC
AACTTTGGGGTGCCATTTGTGCTGTATTCCGCAGCTGGATGAACGAACGCGCCATTCTTTACCGCAAAATGGAAGGTATT
CCTGCCGAATGGGGTACTGCTGTAAGCGTACAAGCCATGGTATTCGGTAACATGGGTGATACTTCGGCTACCGGTGTTTG
CTTCTCTCGTGACGCAGGTAACGGTGAGGATTTGTTTAATGGCGAATATTTGATCAACGCACAAGGTGAAGATGTTGTGG
CCGGTATCCGTACCCCGCAACAAATCACAAAAATCGGTTCTCAGCGTTGGGCAGAACGTGCAGGTATTTCTGAAGAAGAA
CGCGTAGCCAAATACCCCTCTATGGAAGAAGCTATGCCGGAAATCTACAAAGAACTGGATGCCCTGCAGACCAAACTGGA
AAACCACTATCGCGACATGCAGGATATGGAATTTACCGTACAGGAAGGTAAATTATGGTTCTTGCAGACCCGTAACGGTA
AACGTACCGGAGCTGCCATGGTAAAAATCGCCATGGACTTGTTGCGCCAGGGCATGATTGATGAAAAAACCGCATTGGAG
CGTTGCGAACCTAATAAATTAGACGAACTGCTTCACCCTGTGTTCGACAAGAAAGCGTTGAAAGAAGCGAAAGTTTTGAC
TCGTGGTTTGCCCGCTTCTCCGGGTGCCGCTACCGGTCAGATTGTGTTCTTCGCCGATGATGCTGCTAAATGGGCTGCCG
ATGGTAAAAAGGTGGTCATGGTACGTATCGAAACTTCGCCGGAAGACCTGGCAGGTATGGCCGTTGCCGAAGGTATCCTG
ACTGCACGTGGCGGTATGACATCTCACGCTGCGGTTGTGGCCCGTGGTATGGGTAAGTGCTGCGTATCGGGTGCTGGTGC
TATCAATGTAGATTATAAGACACGTACCGTAGAAATTGAAGGTATTACTTTAAAAGAAGGTGACTTCATCTCTCTGAACG
GTACTACCGGTGAAGTATATAAAGGTAAAGTGGAAACCAAAGCAGCCGAAGTTTCAGGCGATTTCGCTGCATTGATGGAT
CTTTGCAACAAATACACAAAGCTGAATGTCCGCACTAACGCTGATACTCCGCATGATGCGGAAGTGGCCCGCGCTTTTGG
TGCTTCAGGTATCGGTCTTTGCCGTACGGAACACATGTTCTTTGATGCTGAAAAGATTGTCGCTATGCGTGAGATGATCC
TGTCACCGGATGTAGAAGGACGTAGAAAAGCATTGGCTAAATTGCTCCCCTTCCAAAAAGCCGACTTCAAAGGTATCTTC
AAGGCTATGGACGGATGTCCGGTAAATGTTCGTCTGCTCGATCCTCCTTTGCATGAGTTCGTTCCCCATGATGCAAAAGG
TCAGGAAGAAATGGCTAAGGCAATGGGCGTAACCGTACAGGAAATCAAAAAACGTGTGGAAAGCCTGTGTGAACACAATC
CGATGTTAGGTCACCGCGGCTGTCGTTTGGGTAACACCTATCCCGAAATCACAGAGATGCAGACTCAAGCGATTCTAGGT
GCTGCAATTGAACTGAAGAAAGAAGGATATGATCCGCATCCCGAAATAATGGTTCCCTTGACCGGTATCCTGTATGAATT
CGAAGCTCAGGAAAAGGTGATCCGTGATGCAGCAGCAGCATTATTTGAAAAAGAAGGTATGGAAATTCCGTTCAAAGTAG
GTACCATGATTGAAATTCCGCGTGCTGCGCTGACAGCCAACCGCATTGCCAGCCGTGCAGAATACTTCTCATTCGGTACC
AATGACTTGACCCAGATGACCTTCGGTTATTCCCGTGACGATATTGCTTCTTTCTTGCCGGTATATCTGGAAAAGAAGAT
TCTAAAAGTAGACCCGTTCCAAGTACTCGACCAAAATGGTGTGGGCCAGCTGATTGAAATGGCTGTAGATAAAGGCCGTT
CAGTTCGTCCTGATTTGAAATGCGGTATCTGCGGTGAGCATGGTGGTGAGCCTTCATCAGTGAAATTCTGTCACAAAGTT
GGTTTGAACTATGTGTCTTGTTCTCCGTTCCGCGTGCCTATCGCCAGACTGGCGGCGGCTCAGGCCGCCGTTGAGGAATA
A

Upstream 100 bases:

>100_bases
TTTCCACTATTTTTTTTCAGTAAAAGATTTGGAGATAACTAAAATATGTATAGTTTTGCGATTACATTTTTTGAGCGAGT
TAACTTTAAATACATTTATT

Downstream 100 bases:

>100_bases
TTCGTTCAACTTTAAATCGTTCAATAAACTTCCGCTTACACTGGGCTTACACCCAAAAGCGAAGTGAATGGTTTTCAGCA
CTATATTAGTGTGGCCCAAG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 906; Mature: 906

Protein sequence:

>906_residues
MDKKRVYTFGNGQAEGKADMRNLLGGKGANLAEMNLIGVPVPPGFTITTEVCSEYYELGKDKVVELLKADVEKAIANIEN
LMNSKFGDVENPLLVSVRSGARASMPGMMDTILNLGLNDEVVEGLSRKTGRPRFAWDSYRRFVQMYGDVVLGMKPVNKED
IDPFEEIIEKVKEEKGVKLDNELEVEDLKELVKRFKVAVKEQTGQDFPTCAYEQLWGAICAVFRSWMNERAILYRKMEGI
PAEWGTAVSVQAMVFGNMGDTSATGVCFSRDAGNGEDLFNGEYLINAQGEDVVAGIRTPQQITKIGSQRWAERAGISEEE
RVAKYPSMEEAMPEIYKELDALQTKLENHYRDMQDMEFTVQEGKLWFLQTRNGKRTGAAMVKIAMDLLRQGMIDEKTALE
RCEPNKLDELLHPVFDKKALKEAKVLTRGLPASPGAATGQIVFFADDAAKWAADGKKVVMVRIETSPEDLAGMAVAEGIL
TARGGMTSHAAVVARGMGKCCVSGAGAINVDYKTRTVEIEGITLKEGDFISLNGTTGEVYKGKVETKAAEVSGDFAALMD
LCNKYTKLNVRTNADTPHDAEVARAFGASGIGLCRTEHMFFDAEKIVAMREMILSPDVEGRRKALAKLLPFQKADFKGIF
KAMDGCPVNVRLLDPPLHEFVPHDAKGQEEMAKAMGVTVQEIKKRVESLCEHNPMLGHRGCRLGNTYPEITEMQTQAILG
AAIELKKEGYDPHPEIMVPLTGILYEFEAQEKVIRDAAAALFEKEGMEIPFKVGTMIEIPRAALTANRIASRAEYFSFGT
NDLTQMTFGYSRDDIASFLPVYLEKKILKVDPFQVLDQNGVGQLIEMAVDKGRSVRPDLKCGICGEHGGEPSSVKFCHKV
GLNYVSCSPFRVPIARLAAAQAAVEE

Sequences:

>Translated_906_residues
MDKKRVYTFGNGQAEGKADMRNLLGGKGANLAEMNLIGVPVPPGFTITTEVCSEYYELGKDKVVELLKADVEKAIANIEN
LMNSKFGDVENPLLVSVRSGARASMPGMMDTILNLGLNDEVVEGLSRKTGRPRFAWDSYRRFVQMYGDVVLGMKPVNKED
IDPFEEIIEKVKEEKGVKLDNELEVEDLKELVKRFKVAVKEQTGQDFPTCAYEQLWGAICAVFRSWMNERAILYRKMEGI
PAEWGTAVSVQAMVFGNMGDTSATGVCFSRDAGNGEDLFNGEYLINAQGEDVVAGIRTPQQITKIGSQRWAERAGISEEE
RVAKYPSMEEAMPEIYKELDALQTKLENHYRDMQDMEFTVQEGKLWFLQTRNGKRTGAAMVKIAMDLLRQGMIDEKTALE
RCEPNKLDELLHPVFDKKALKEAKVLTRGLPASPGAATGQIVFFADDAAKWAADGKKVVMVRIETSPEDLAGMAVAEGIL
TARGGMTSHAAVVARGMGKCCVSGAGAINVDYKTRTVEIEGITLKEGDFISLNGTTGEVYKGKVETKAAEVSGDFAALMD
LCNKYTKLNVRTNADTPHDAEVARAFGASGIGLCRTEHMFFDAEKIVAMREMILSPDVEGRRKALAKLLPFQKADFKGIF
KAMDGCPVNVRLLDPPLHEFVPHDAKGQEEMAKAMGVTVQEIKKRVESLCEHNPMLGHRGCRLGNTYPEITEMQTQAILG
AAIELKKEGYDPHPEIMVPLTGILYEFEAQEKVIRDAAAALFEKEGMEIPFKVGTMIEIPRAALTANRIASRAEYFSFGT
NDLTQMTFGYSRDDIASFLPVYLEKKILKVDPFQVLDQNGVGQLIEMAVDKGRSVRPDLKCGICGEHGGEPSSVKFCHKV
GLNYVSCSPFRVPIARLAAAQAAVEE
>Mature_906_residues
MDKKRVYTFGNGQAEGKADMRNLLGGKGANLAEMNLIGVPVPPGFTITTEVCSEYYELGKDKVVELLKADVEKAIANIEN
LMNSKFGDVENPLLVSVRSGARASMPGMMDTILNLGLNDEVVEGLSRKTGRPRFAWDSYRRFVQMYGDVVLGMKPVNKED
IDPFEEIIEKVKEEKGVKLDNELEVEDLKELVKRFKVAVKEQTGQDFPTCAYEQLWGAICAVFRSWMNERAILYRKMEGI
PAEWGTAVSVQAMVFGNMGDTSATGVCFSRDAGNGEDLFNGEYLINAQGEDVVAGIRTPQQITKIGSQRWAERAGISEEE
RVAKYPSMEEAMPEIYKELDALQTKLENHYRDMQDMEFTVQEGKLWFLQTRNGKRTGAAMVKIAMDLLRQGMIDEKTALE
RCEPNKLDELLHPVFDKKALKEAKVLTRGLPASPGAATGQIVFFADDAAKWAADGKKVVMVRIETSPEDLAGMAVAEGIL
TARGGMTSHAAVVARGMGKCCVSGAGAINVDYKTRTVEIEGITLKEGDFISLNGTTGEVYKGKVETKAAEVSGDFAALMD
LCNKYTKLNVRTNADTPHDAEVARAFGASGIGLCRTEHMFFDAEKIVAMREMILSPDVEGRRKALAKLLPFQKADFKGIF
KAMDGCPVNVRLLDPPLHEFVPHDAKGQEEMAKAMGVTVQEIKKRVESLCEHNPMLGHRGCRLGNTYPEITEMQTQAILG
AAIELKKEGYDPHPEIMVPLTGILYEFEAQEKVIRDAAAALFEKEGMEIPFKVGTMIEIPRAALTANRIASRAEYFSFGT
NDLTQMTFGYSRDDIASFLPVYLEKKILKVDPFQVLDQNGVGQLIEMAVDKGRSVRPDLKCGICGEHGGEPSSVKFCHKV
GLNYVSCSPFRVPIARLAAAQAAVEE

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=453, Percent_Identity=24.7240618101545, Blast_Score=106, Evalue=8e-24,
Organism=Escherichia coli, GI1789193, Length=460, Percent_Identity=23.0434782608696, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1788726, Length=447, Percent_Identity=22.8187919463087, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 99848; Mature: 99848

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKRVYTFGNGQAEGKADMRNLLGGKGANLAEMNLIGVPVPPGFTITTEVCSEYYELGK
CCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHCCH
DKVVELLKADVEKAIANIENLMNSKFGDVENPLLVSVRSGARASMPGMMDTILNLGLNDE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHH
VVEGLSRKTGRPRFAWDSYRRFVQMYGDVVLGMKPVNKEDIDPFEEIIEKVKEEKGVKLD
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCCCC
NELEVEDLKELVKRFKVAVKEQTGQDFPTCAYEQLWGAICAVFRSWMNERAILYRKMEGI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
PAEWGTAVSVQAMVFGNMGDTSATGVCFSRDAGNGEDLFNGEYLINAQGEDVVAGIRTPQ
CCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCCCEEECCCCHH
QITKIGSQRWAERAGISEEERVAKYPSMEEAMPEIYKELDALQTKLENHYRDMQDMEFTV
HHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEE
QEGKLWFLQTRNGKRTGAAMVKIAMDLLRQGMIDEKTALERCEPNKLDELLHPVFDKKAL
ECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
KEAKVLTRGLPASPGAATGQIVFFADDAAKWAADGKKVVMVRIETSPEDLAGMAVAEGIL
HHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHH
TARGGMTSHAAVVARGMGKCCVSGAGAINVDYKTRTVEIEGITLKEGDFISLNGTTGEVY
HHCCCCCHHHHHHHHHHHHHHHCCCCEEEECEEEEEEEEEEEEECCCCEEEECCCCCCEE
KGKVETKAAEVSGDFAALMDLCNKYTKLNVRTNADTPHDAEVARAFGASGIGLCRTEHMF
ECCHHHHHHHHCCHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCCCCCCEEHHHHH
FDAEKIVAMREMILSPDVEGRRKALAKLLPFQKADFKGIFKAMDGCPVNVRLLDPPLHEF
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHH
VPHDAKGQEEMAKAMGVTVQEIKKRVESLCEHNPMLGHRGCRLGNTYPEITEMQTQAILG
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AAIELKKEGYDPHPEIMVPLTGILYEFEAQEKVIRDAAAALFEKEGMEIPFKVGTMIEIP
HHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEECC
RAALTANRIASRAEYFSFGTNDLTQMTFGYSRDDIASFLPVYLEKKILKVDPFQVLDQNG
HHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
VGQLIEMAVDKGRSVRPDLKCGICGEHGGEPSSVKFCHKVGLNYVSCSPFRVPIARLAAA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHH
QAAVEE
HHHHCC
>Mature Secondary Structure
MDKKRVYTFGNGQAEGKADMRNLLGGKGANLAEMNLIGVPVPPGFTITTEVCSEYYELGK
CCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHCCH
DKVVELLKADVEKAIANIENLMNSKFGDVENPLLVSVRSGARASMPGMMDTILNLGLNDE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHH
VVEGLSRKTGRPRFAWDSYRRFVQMYGDVVLGMKPVNKEDIDPFEEIIEKVKEEKGVKLD
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCCCC
NELEVEDLKELVKRFKVAVKEQTGQDFPTCAYEQLWGAICAVFRSWMNERAILYRKMEGI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
PAEWGTAVSVQAMVFGNMGDTSATGVCFSRDAGNGEDLFNGEYLINAQGEDVVAGIRTPQ
CCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCCCEEECCCCHH
QITKIGSQRWAERAGISEEERVAKYPSMEEAMPEIYKELDALQTKLENHYRDMQDMEFTV
HHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEE
QEGKLWFLQTRNGKRTGAAMVKIAMDLLRQGMIDEKTALERCEPNKLDELLHPVFDKKAL
ECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
KEAKVLTRGLPASPGAATGQIVFFADDAAKWAADGKKVVMVRIETSPEDLAGMAVAEGIL
HHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHH
TARGGMTSHAAVVARGMGKCCVSGAGAINVDYKTRTVEIEGITLKEGDFISLNGTTGEVY
HHCCCCCHHHHHHHHHHHHHHHCCCCEEEECEEEEEEEEEEEEECCCCEEEECCCCCCEE
KGKVETKAAEVSGDFAALMDLCNKYTKLNVRTNADTPHDAEVARAFGASGIGLCRTEHMF
ECCHHHHHHHHCCHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCCCCCCEEHHHHH
FDAEKIVAMREMILSPDVEGRRKALAKLLPFQKADFKGIFKAMDGCPVNVRLLDPPLHEF
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHH
VPHDAKGQEEMAKAMGVTVQEIKKRVESLCEHNPMLGHRGCRLGNTYPEITEMQTQAILG
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AAIELKKEGYDPHPEIMVPLTGILYEFEAQEKVIRDAAAALFEKEGMEIPFKVGTMIEIP
HHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEECC
RAALTANRIASRAEYFSFGTNDLTQMTFGYSRDDIASFLPVYLEKKILKVDPFQVLDQNG
HHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
VGQLIEMAVDKGRSVRPDLKCGICGEHGGEPSSVKFCHKVGLNYVSCSPFRVPIARLAAA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHH
QAAVEE
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]