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The map label for this gene is mtbC1 [H]

Identifier: 150005447

GI number: 150005447

Start: 3719948

End: 3721750

Strand: Reverse

Name: mtbC1 [H]

Synonym: BVU_2930

Alternate gene names: 150005447

Gene position: 3721750-3719948 (Counterclockwise)

Preceding gene: 150005448

Following gene: 150005446

Centisome position: 72.08

GC content: 48.92

Gene sequence:

>1803_bases
ATGGAAACTTGGAAAACGAATTTAGACGAGACAAAGAAACGATATATAGATTGGTGGAACCATAAAGGAATCATACTGAA
CATGTGGGAGCACTTTCAGGAAGGTGTGAAACCTCATGCGGATATCCCTGCACCCTCCCCGGCAAAAGACCTGAATCAGA
AATGGTTCGATCCGCAATGGCGTGCAGAATACCTGGACTGGTACGTGGCACACAGCAGTCTGAAAGCAGATATCCTGCCG
GTAGCCAATACCCAGTTAGGCCCCGGTTCATTGGCAGCCATCTTGGGCGGCGTGTTCGAAGGAGGGGAAGATACCATTTG
GATTCATCCCGATCCCGATTTCAACGATGAGATTGTTTTCAATCCCGAGCATCCCAACTGGATCTTGCATAAAGAATTGC
TGAAGGCATGCAAGGCGAAAGCAAACGGTCACTATTACGTAGGAATGCCCGATTTGATGGAAGGGCTGGACGTACTGGCC
GCCTTGAAAGGTACAGACAAAGTTTTGCTGGATACCGTGATGCAGCCCGAAGTGCTGGAACAGCAAATGCAACAAATCAA
TGATATCTATTTCAAAGTCTTTGATGAACTTTACGACATCATCCGTGAAGGGGACGAAATGGCTTTCTGCTATTTTTCTT
CATGGGCTCCCGGTAAAATGAGCAAATTACAAAGCGATATTTCTACCATGATCAGCGAAGATGATTATCGTCGCTTCGTG
CAACCGTTCATCCGCGAACAATGCCAAAAGATAGACTACACCCTGTATCATTTGGACGGTGTGGGCGCCATGCATCACCT
GCCCGCCCTTCTTGAAATTGAGGAACTGAATGCCATTCAATGGACTCCGGGCGTAGGTGAGCCACAAGGAGGATCACCCA
AATGGTACGACCTTTACAAGAAGATCCTTGCCGGAGGCAAAAGTATCATGGCTTGTTGGGTAACCTTGGACGAACTGAAA
CCCTTGCTGGACAATATCGGTGCGGACGGTGTGCATCTGGAAATGGACTTCCATAACGAGAGAGAAGTGGAACAAGCGAT
GCGCATTGTTGAGGAATACTCCTCTTCCTCCTCTGACGTATCCAAAGAAGACCTTTTGAAAGAAGAAGCCGCTGCAACAA
AACAAGAAACAATCGTCGTCAAAGACTCTGCGGCAGCCGGGAATGAGGCCTTGAAACCTTTGTTCAATGCCATTGTGGAC
GGAAAGCTGGAACCGGCCATAGAAGTAACCAAGAAAGCCATCGCCGAAGGAGTACAGCCCCAGGAAATTATCAACAACTA
CATGATAAAGGCTATGGGCGAAGTGGGACAACGCTTCCAGGACGGAAAAGCGTTCGTGCCCCAACTGCTGATGGCAGGAC
GCGCCATGAAGGGAGCTTTGGAACTTCTGAAGCCTTTATTGCAAGGGAGTGCCTCGACCACCATAGGCAAGGTGGTCATC
GGGACCGTGAAAGGCGACTTGCATGATATCGGCAAGAACCTAGTAGCTTCCATGCTGGAAGGCTGCGGCTTTGAGGTGAT
CAACATAGGCATAGATGTATCTTGCGACAAATTTGTGGAAGAGGTCAAGAAAAACAAGGCAGACATCCTGTGCATGAGCG
CCTTGCTGACAACAACCATGACTTATATGCAGGAGGTGATCAACGCTTTGGAAGCAGCCGGTATCCGTCATCAGGTAAAA
GTGATGATAGGCGGCGCTCCGGTAAGCCAGGGATTTGCCGATGAAATAGGTGCAGACGGGTATAGCGACAATGCCAATAC
GGCCGTAGCGGTAGCCAAGGAACTGATGGGGCTTCATAAATAA

Upstream 100 bases:

>100_bases
ATCCGGATGAAGTGGAATGCACGGACGATATGGATTTAAGCAAAAGCATCGGTCTACACATAGACCCTCATTTTAAAGGA
ATATTTAAGAACTGACGAAT

Downstream 100 bases:

>100_bases
ACGGAATATTCATCTTAATCTGATACATGAATTATGCACGCTAATTATTTAGATACGTTAGATTGGGGCATTCTTATTGC
CTACTTTCTGATTCTTATAG

Product: trimethylamine corrinoid protein 2

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
METWKTNLDETKKRYIDWWNHKGIILNMWEHFQEGVKPHADIPAPSPAKDLNQKWFDPQWRAEYLDWYVAHSSLKADILP
VANTQLGPGSLAAILGGVFEGGEDTIWIHPDPDFNDEIVFNPEHPNWILHKELLKACKAKANGHYYVGMPDLMEGLDVLA
ALKGTDKVLLDTVMQPEVLEQQMQQINDIYFKVFDELYDIIREGDEMAFCYFSSWAPGKMSKLQSDISTMISEDDYRRFV
QPFIREQCQKIDYTLYHLDGVGAMHHLPALLEIEELNAIQWTPGVGEPQGGSPKWYDLYKKILAGGKSIMACWVTLDELK
PLLDNIGADGVHLEMDFHNEREVEQAMRIVEEYSSSSSDVSKEDLLKEEAAATKQETIVVKDSAAAGNEALKPLFNAIVD
GKLEPAIEVTKKAIAEGVQPQEIINNYMIKAMGEVGQRFQDGKAFVPQLLMAGRAMKGALELLKPLLQGSASTTIGKVVI
GTVKGDLHDIGKNLVASMLEGCGFEVINIGIDVSCDKFVEEVKKNKADILCMSALLTTTMTYMQEVINALEAAGIRHQVK
VMIGGAPVSQGFADEIGADGYSDNANTAVAVAKELMGLHK

Sequences:

>Translated_600_residues
METWKTNLDETKKRYIDWWNHKGIILNMWEHFQEGVKPHADIPAPSPAKDLNQKWFDPQWRAEYLDWYVAHSSLKADILP
VANTQLGPGSLAAILGGVFEGGEDTIWIHPDPDFNDEIVFNPEHPNWILHKELLKACKAKANGHYYVGMPDLMEGLDVLA
ALKGTDKVLLDTVMQPEVLEQQMQQINDIYFKVFDELYDIIREGDEMAFCYFSSWAPGKMSKLQSDISTMISEDDYRRFV
QPFIREQCQKIDYTLYHLDGVGAMHHLPALLEIEELNAIQWTPGVGEPQGGSPKWYDLYKKILAGGKSIMACWVTLDELK
PLLDNIGADGVHLEMDFHNEREVEQAMRIVEEYSSSSSDVSKEDLLKEEAAATKQETIVVKDSAAAGNEALKPLFNAIVD
GKLEPAIEVTKKAIAEGVQPQEIINNYMIKAMGEVGQRFQDGKAFVPQLLMAGRAMKGALELLKPLLQGSASTTIGKVVI
GTVKGDLHDIGKNLVASMLEGCGFEVINIGIDVSCDKFVEEVKKNKADILCMSALLTTTMTYMQEVINALEAAGIRHQVK
VMIGGAPVSQGFADEIGADGYSDNANTAVAVAKELMGLHK
>Mature_600_residues
METWKTNLDETKKRYIDWWNHKGIILNMWEHFQEGVKPHADIPAPSPAKDLNQKWFDPQWRAEYLDWYVAHSSLKADILP
VANTQLGPGSLAAILGGVFEGGEDTIWIHPDPDFNDEIVFNPEHPNWILHKELLKACKAKANGHYYVGMPDLMEGLDVLA
ALKGTDKVLLDTVMQPEVLEQQMQQINDIYFKVFDELYDIIREGDEMAFCYFSSWAPGKMSKLQSDISTMISEDDYRRFV
QPFIREQCQKIDYTLYHLDGVGAMHHLPALLEIEELNAIQWTPGVGEPQGGSPKWYDLYKKILAGGKSIMACWVTLDELK
PLLDNIGADGVHLEMDFHNEREVEQAMRIVEEYSSSSSDVSKEDLLKEEAAATKQETIVVKDSAAAGNEALKPLFNAIVD
GKLEPAIEVTKKAIAEGVQPQEIINNYMIKAMGEVGQRFQDGKAFVPQLLMAGRAMKGALELLKPLLQGSASTTIGKVVI
GTVKGDLHDIGKNLVASMLEGCGFEVINIGIDVSCDKFVEEVKKNKADILCMSALLTTTMTYMQEVINALEAAGIRHQVK
VMIGGAPVSQGFADEIGADGYSDNANTAVAVAKELMGLHK

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=197, Percent_Identity=32.994923857868, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1790450, Length=216, Percent_Identity=32.8703703703704, Blast_Score=115, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17535405, Length=176, Percent_Identity=31.25, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 66673; Mature: 66673

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METWKTNLDETKKRYIDWWNHKGIILNMWEHFQEGVKPHADIPAPSPAKDLNQKWFDPQW
CCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHH
RAEYLDWYVAHSSLKADILPVANTQLGPGSLAAILGGVFEGGEDTIWIHPDPDFNDEIVF
HHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEE
NPEHPNWILHKELLKACKAKANGHYYVGMPDLMEGLDVLAALKGTDKVLLDTVMQPEVLE
CCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
QQMQQINDIYFKVFDELYDIIREGDEMAFCYFSSWAPGKMSKLQSDISTMISEDDYRRFV
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHH
QPFIREQCQKIDYTLYHLDGVGAMHHLPALLEIEELNAIQWTPGVGEPQGGSPKWYDLYK
HHHHHHHHHHCCCEEEEECCCCHHHHCCHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHH
KILAGGKSIMACWVTLDELKPLLDNIGADGVHLEMDFHNEREVEQAMRIVEEYSSSSSDV
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
SKEDLLKEEAAATKQETIVVKDSAAAGNEALKPLFNAIVDGKLEPAIEVTKKAIAEGVQP
CHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCH
QEIINNYMIKAMGEVGQRFQDGKAFVPQLLMAGRAMKGALELLKPLLQGSASTTIGKVVI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHEE
GTVKGDLHDIGKNLVASMLEGCGFEVINIGIDVSCDKFVEEVKKNKADILCMSALLTTTM
EHHCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
TYMQEVINALEAAGIRHQVKVMIGGAPVSQGFADEIGADGYSDNANTAVAVAKELMGLHK
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
METWKTNLDETKKRYIDWWNHKGIILNMWEHFQEGVKPHADIPAPSPAKDLNQKWFDPQW
CCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHH
RAEYLDWYVAHSSLKADILPVANTQLGPGSLAAILGGVFEGGEDTIWIHPDPDFNDEIVF
HHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEE
NPEHPNWILHKELLKACKAKANGHYYVGMPDLMEGLDVLAALKGTDKVLLDTVMQPEVLE
CCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
QQMQQINDIYFKVFDELYDIIREGDEMAFCYFSSWAPGKMSKLQSDISTMISEDDYRRFV
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHH
QPFIREQCQKIDYTLYHLDGVGAMHHLPALLEIEELNAIQWTPGVGEPQGGSPKWYDLYK
HHHHHHHHHHCCCEEEEECCCCHHHHCCHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHH
KILAGGKSIMACWVTLDELKPLLDNIGADGVHLEMDFHNEREVEQAMRIVEEYSSSSSDV
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
SKEDLLKEEAAATKQETIVVKDSAAAGNEALKPLFNAIVDGKLEPAIEVTKKAIAEGVQP
CHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCH
QEIINNYMIKAMGEVGQRFQDGKAFVPQLLMAGRAMKGALELLKPLLQGSASTTIGKVVI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHEE
GTVKGDLHDIGKNLVASMLEGCGFEVINIGIDVSCDKFVEEVKKNKADILCMSALLTTTM
EHHCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
TYMQEVINALEAAGIRHQVKVMIGGAPVSQGFADEIGADGYSDNANTAVAVAKELMGLHK
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]