BacMap

Definition	Mycobacterium tuberculosis F11, complete genome.
Accession	NC_009565
Length	4,424,435

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The map label for this gene is ephD [H]

Identifier: 148824249

GI number: 148824249

Start: 3429495

End: 3430358

Strand: Reverse

Name: ephD [H]

Synonym: TBFG_13074

Alternate gene names: 148824249

Gene position: 3430358-3429495 (Counterclockwise)

Preceding gene: 148824250

Following gene: 148824246

Centisome position: 77.53

GC content: 64.7

Gene sequence:

>864_bases
ATGTTGCAGAGGGGAGCCGGACAGTATTTCGCGGGTAAGCGGTGCTTTGTCACCGGCGCGGCCAGTGGCATCGGTCGCGC
GACCGCGTTGCGCCTCGCGGCGCAAGGTGCCGAGCTGTATCTGACCGACCGCGACCGTGATGGCTTGGCGCAAACCGTGT
GTGACGCCCGTGCACTCGGCGCTCAGGTGCCCGAGCATCGAGTTCTGGACGTCTCCGACTACCAGGACGTGGCGGCATTC
GCGGCCGATATCCACGCCCGTCATCCCAGCATGGACGTGGTACTAAACATCGCCGGTGTGTCGGCCTGGGGCACCGTTGA
CCAGCTCACGCATGCTCAGTGGAGCAGGATGGTCGCGATCAATCTCATGGGCCCAATCCACGTCATCGAGACCTTGGTCC
CACCGATGGTCGCCGCCGGTCGGGGCGGGCACCTGGTCAATGTGTCCTCGGCGGCCGGGCTGGTTGGCTTGCCGTGGCAT
GCGGCCTATAGCGCTAGCAAGTACGGGTTGCGGGGACTTTCTGAGGTGCTGCGCTTCGATCTGGCCCGGCACGGCATCGG
GGTGTCGGTCGTGGTGCCTGGCGCCGTCAAGACCCCGCTGGTCAATACGGTCGAGATCGCCGGAGTGGATCGCGACGACC
CGAGGGTCAACCGCTGGGTCGAACGGTTCAGTGGTCACGCCGTGACGCCGGAGAAGGCGGCCGACAAAATTTTGGCCGGG
GTCACAAGGAACAGATACCTGGTCTATACGTCGGCGGACATCCGGGCGCTGTATGCGTTCAAGCGATATGCGTGGTGGCC
ATACACCCTGGTGATGCGACGAGTCAACGTGTTCTTCACGCGCGCGCTTCGGCCCGGGCCATAG

Upstream 100 bases:

>100_bases
GCTCAACGCGCTTTGGGGTGGCGGCAAGGCCCCATAGCTATCGGGGCGCATCGATCGTCCTACCGGAGGGGGTGTCGGTA
CCGCCGGTATCGTCACGACC

Downstream 100 bases:

>100_bases
CGGCCGGTGCGGTCAAGACTTCGCGCCGCCCAGGTGCGGTGTGCCCGCCGGTTCTAGGCCAGTTCTAACCGCACTCCCAG
CAACCGGACGGGCCGATCCA

Product: short chain dehydrogenase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MLQRGAGQYFAGKRCFVTGAASGIGRATALRLAAQGAELYLTDRDRDGLAQTVCDARALGAQVPEHRVLDVSDYQDVAAF
AADIHARHPSMDVVLNIAGVSAWGTVDQLTHAQWSRMVAINLMGPIHVIETLVPPMVAAGRGGHLVNVSSAAGLVGLPWH
AAYSASKYGLRGLSEVLRFDLARHGIGVSVVVPGAVKTPLVNTVEIAGVDRDDPRVNRWVERFSGHAVTPEKAADKILAG
VTRNRYLVYTSADIRALYAFKRYAWWPYTLVMRRVNVFFTRALRPGP

Sequences:

>Translated_287_residues
MLQRGAGQYFAGKRCFVTGAASGIGRATALRLAAQGAELYLTDRDRDGLAQTVCDARALGAQVPEHRVLDVSDYQDVAAF
AADIHARHPSMDVVLNIAGVSAWGTVDQLTHAQWSRMVAINLMGPIHVIETLVPPMVAAGRGGHLVNVSSAAGLVGLPWH
AAYSASKYGLRGLSEVLRFDLARHGIGVSVVVPGAVKTPLVNTVEIAGVDRDDPRVNRWVERFSGHAVTPEKAADKILAG
VTRNRYLVYTSADIRALYAFKRYAWWPYTLVMRRVNVFFTRALRPGP
>Mature_287_residues
MLQRGAGQYFAGKRCFVTGAASGIGRATALRLAAQGAELYLTDRDRDGLAQTVCDARALGAQVPEHRVLDVSDYQDVAAF
AADIHARHPSMDVVLNIAGVSAWGTVDQLTHAQWSRMVAINLMGPIHVIETLVPPMVAAGRGGHLVNVSSAAGLVGLPWH
AAYSASKYGLRGLSEVLRFDLARHGIGVSVVVPGAVKTPLVNTVEIAGVDRDDPRVNRWVERFSGHAVTPEKAADKILAG
VTRNRYLVYTSADIRALYAFKRYAWWPYTLVMRRVNVFFTRALRPGP

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=252, Percent_Identity=31.7460317460317, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI20149619, Length=234, Percent_Identity=29.0598290598291, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4503817, Length=239, Percent_Identity=28.8702928870293, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI142976729, Length=266, Percent_Identity=24.812030075188, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI40807363, Length=256, Percent_Identity=26.171875, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI32483357, Length=190, Percent_Identity=31.5789473684211, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4758504, Length=200, Percent_Identity=34, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI210032110, Length=257, Percent_Identity=22.568093385214, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI31542939, Length=202, Percent_Identity=31.6831683168317, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI223718074, Length=194, Percent_Identity=32.9896907216495, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI50726952, Length=190, Percent_Identity=32.1052631578947, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI7657478, Length=214, Percent_Identity=31.3084112149533, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI7706318, Length=194, Percent_Identity=28.3505154639175, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI40254992, Length=219, Percent_Identity=30.1369863013699, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI157739938, Length=243, Percent_Identity=24.2798353909465, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI150247226, Length=257, Percent_Identity=29.9610894941634, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI66933014, Length=189, Percent_Identity=32.2751322751323, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI32455239, Length=196, Percent_Identity=29.5918367346939, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI5031765, Length=196, Percent_Identity=29.5918367346939, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI33667109, Length=202, Percent_Identity=30.1980198019802, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI19743808, Length=206, Percent_Identity=28.6407766990291, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI7705925, Length=192, Percent_Identity=28.125, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI109715829, Length=254, Percent_Identity=26.3779527559055, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI5031737, Length=194, Percent_Identity=30.4123711340206, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI83715985, Length=182, Percent_Identity=33.5164835164835, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI209862901, Length=261, Percent_Identity=27.2030651340996, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI19923983, Length=261, Percent_Identity=27.2030651340996, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI126723191, Length=183, Percent_Identity=29.5081967213115, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI224922801, Length=172, Percent_Identity=31.9767441860465, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI126723750, Length=195, Percent_Identity=29.2307692307692, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI44680136, Length=195, Percent_Identity=25.6410256410256, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI44680133, Length=195, Percent_Identity=25.6410256410256, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI17738292, Length=195, Percent_Identity=25.6410256410256, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI40548397, Length=200, Percent_Identity=28.5, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI214010158, Length=200, Percent_Identity=28.5, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI214010156, Length=200, Percent_Identity=28.5, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI22758144, Length=200, Percent_Identity=28.5, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787335, Length=206, Percent_Identity=31.5533980582524, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786812, Length=235, Percent_Identity=30.2127659574468, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786701, Length=228, Percent_Identity=28.5087719298246, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788459, Length=186, Percent_Identity=29.5698924731183, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082100, Length=202, Percent_Identity=31.1881188118812, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082160, Length=210, Percent_Identity=25.2380952380952, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI2367175, Length=189, Percent_Identity=31.7460317460317, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790717, Length=193, Percent_Identity=26.4248704663212, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789057, Length=193, Percent_Identity=24.8704663212435, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787820, Length=223, Percent_Identity=25.5605381165919, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787905, Length=193, Percent_Identity=27.4611398963731, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789208, Length=189, Percent_Identity=23.8095238095238, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=196, Percent_Identity=31.1224489795918, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17564282, Length=196, Percent_Identity=27.0408163265306, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17560676, Length=222, Percent_Identity=27.4774774774775, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17567345, Length=193, Percent_Identity=26.9430051813472, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17570069, Length=264, Percent_Identity=27.6515151515151, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17507613, Length=202, Percent_Identity=31.1881188118812, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32563809, Length=202, Percent_Identity=31.1881188118812, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193203117, Length=237, Percent_Identity=24.0506329113924, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193203115, Length=236, Percent_Identity=24.5762711864407, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25147288, Length=195, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71997402, Length=273, Percent_Identity=27.1062271062271, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17562906, Length=200, Percent_Identity=32.5, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI115534694, Length=191, Percent_Identity=30.3664921465969, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17562910, Length=226, Percent_Identity=26.9911504424779, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=206, Percent_Identity=30.0970873786408, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560150, Length=245, Percent_Identity=27.3469387755102, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=201, Percent_Identity=29.8507462686567, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=200, Percent_Identity=34, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17538486, Length=197, Percent_Identity=30.4568527918782, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=201, Percent_Identity=28.3582089552239, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562990, Length=197, Percent_Identity=27.9187817258883, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=202, Percent_Identity=31.1881188118812, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=196, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=198, Percent_Identity=29.7979797979798, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=193, Percent_Identity=23.3160621761658, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=199, Percent_Identity=29.1457286432161, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=196, Percent_Identity=26.0204081632653, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=206, Percent_Identity=27.6699029126214, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322861, Length=193, Percent_Identity=27.979274611399, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322067, Length=188, Percent_Identity=27.1276595744681, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24644339, Length=191, Percent_Identity=31.9371727748691, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21358495, Length=266, Percent_Identity=28.1954887218045, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21356301, Length=239, Percent_Identity=26.3598326359833, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357041, Length=191, Percent_Identity=29.8429319371728, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571526, Length=191, Percent_Identity=29.8429319371728, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355631, Length=246, Percent_Identity=28.4552845528455, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24639444, Length=197, Percent_Identity=30.9644670050761, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17737361, Length=197, Percent_Identity=33.502538071066, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281364892, Length=220, Percent_Identity=29.5454545454545, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640492, Length=193, Percent_Identity=26.9430051813472, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI23397609, Length=191, Percent_Identity=31.413612565445, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24643142, Length=194, Percent_Identity=32.4742268041237, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24649181, Length=197, Percent_Identity=27.4111675126904, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355319, Length=197, Percent_Identity=27.4111675126904, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221510726, Length=239, Percent_Identity=25.9414225941423, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24581726, Length=255, Percent_Identity=25.4901960784314, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221331218, Length=199, Percent_Identity=30.6532663316583, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24665243, Length=199, Percent_Identity=30.6532663316583, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 31110; Mature: 31110

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQRGAGQYFAGKRCFVTGAASGIGRATALRLAAQGAELYLTDRDRDGLAQTVCDARALG
CCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
AQVPEHRVLDVSDYQDVAAFAADIHARHPSMDVVLNIAGVSAWGTVDQLTHAQWSRMVAI
CCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHEEEE
NLMGPIHVIETLVPPMVAAGRGGHLVNVSSAAGLVGLPWHAAYSASKYGLRGLSEVLRFD
EECCHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCHHHHCCCHHHHHHHHHHHHHH
LARHGIGVSVVVPGAVKTPLVNTVEIAGVDRDDPRVNRWVERFSGHAVTPEKAADKILAG
HHHCCCCEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
VTRNRYLVYTSADIRALYAFKRYAWWPYTLVMRRVNVFFTRALRPGP
CCCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLQRGAGQYFAGKRCFVTGAASGIGRATALRLAAQGAELYLTDRDRDGLAQTVCDARALG
CCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
AQVPEHRVLDVSDYQDVAAFAADIHARHPSMDVVLNIAGVSAWGTVDQLTHAQWSRMVAI
CCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHEEEE
NLMGPIHVIETLVPPMVAAGRGGHLVNVSSAAGLVGLPWHAAYSASKYGLRGLSEVLRFD
EECCHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCHHHHCCCHHHHHHHHHHHHHH
LARHGIGVSVVVPGAVKTPLVNTVEIAGVDRDDPRVNRWVERFSGHAVTPEKAADKILAG
HHHCCCCEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
VTRNRYLVYTSADIRALYAFKRYAWWPYTLVMRRVNVFFTRALRPGP
CCCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]